简介：摘要随着汽车电子技术的不断发展，汽车上各种电子控制单元的数目不断增加，传统汽车电子控制单元(ElectronicControlunit)间采用点对点的通信方式，导致车内连接导线显著增加并且布线复杂，所以提高各个控制单元间通讯可靠性和降低导线成本就成为迫切需要解决的问题。为此德国BOSCH公司开发了CAN总线协议，并使其成为国际标准(ISO11898)。

简介：BackgroundApulmonary-infectedpatienthadseverehyponatremiathatlastedtwodaysduringhospitalization.Wecouldnotfindanyhistorysuchasgluttony,liverandgalldisease.Theexaminationofliverfunctionreportedthatthehepaticenzymologyisnormal.InICU,wequicklyfoundhemodiastasereachedatextremelyhighlevel.TheberryCTindicatedthattheheadofpancreasswellingandtheboundarywasobscureandeffusive.Thepatientwasdiagnosedasacutepancreatitis.Thiscaseraisesaquestiontous:Doesseverehyponatremiacauseacutepancreatitis?Ithasnotbeenreported.MethodsWeestablishedacuteanimalmodelofhyponatremiathatdidnotincludeanyotherelectrolytechaos.Aftertheanimalmodelofhyponatraemiacompleted,serumamylaselevelsandpathologicalexaminationwereanalyzed.ResultsThehyponatremiamodels,outof16rats,onlyfourrats'serumsodiumwerelessthan120mmol/L.Inthesefourrats,besidesserumsodium,theremainingelectrolyteswerenormalandserumlipidswereinnormalrange,onlyonerat'sserumamylaseincreasedsignificantlymorethanfourtimesofthenormalcontrolgroupanditspathologicalfindingshintedpancreaticedema.ConclusionsTheexperimentalmodelsuccessfullyduplicatedtheclinicalandpathologicfeaturesofpancreatitisinducedbyhyponatraemia.Throughthiscasereport,wehopethatattentioncanbepaidtochangesinserumamylasewhenhyponatremiaappeared.

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简介：Anesthesiaisanimportantpartofmedicine:theabilitytopreventpainand/orunwantedmovementduringsurgeryiscriticaltomakingtherequiredincisionsandinjectionsortoemployotherpotentiallypainfulprocedureswithoutcausingthepatientunduedistress.Patientswithchronicpain,especiallythosewithneuralgia(amalfunctioningnervethatconstantlysignalspain),alsorelyon

简介：随着循证实践的蓬勃发展,医疗各个领域的临床实践指南日益增多,在规范临床实践、改善患者结局和优化资源配置等方面发挥了重要作用。目前,构建临床实践指南的方法主要有制定新指南和整合现有指南。针对某个健康问题,如果目前已经有相关的指南,则可以考虑进行指南整合。指南整合是指基于系统的、透明的方法,使用和/或修改现有的指南,以便将指南应用于其他临床环境。

简介：ItwasreportedthatProf.YUandhiscolleaguesfromtheFirstAttachedHospitalofHarbinMedicalUniversityhadachievedanewdevelopmentinelectroacupuncture(EA)treatmentofurinarylithiasisinclinic.Bycombiningthemeridian-collateraltheoryoftraditionalChinesemedicine(TCM)andthesegmentaldistributionofthespinalnerveroots

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简介：Objective:Toobservetheeffectsofthermalstressonproliferationofhumanvascularendothelialcells(VECs)andexploreitssignificance.Methods:ChangesofVECsproliferationwereinvestigatedwith3H-TdRincorporationmethodafterECV304wastreatedat43℃for2hours,whileexpressionsofintercellularadhesionmolecule-1(ICAM-1),inhibitorofdifferentiation-1(ID1),andP16andP21proteinsweredeterminedbyWesternBlotting.Results:TheeffectofinhibitionofVECsgrowthafterthermalstresswasdetectedby3H-TdRincorporationexperiment.WesternblottingshowedICAM-1,amarkerofactivatedendothelialcells,wasincreasedmarkedlyafterthermalstress.ExpressionofID1proteindeclinedgraduallywithincreasingexpressionsofitsdownstreamgenes,P16andP21followingthethermalstress.Conclusions:ThermalstresscouldstronglyactivateVECsandinhibitproliferationofVECsthroughID1,thusdownregulatingcyclin-dependentkinaseinhibitors,P16andP21,whichmightbeanessentialpathwayforrecoveryofVECsafterthermalstress.

简介：Canprogesteronebeabetteralternativetodexamethasoneforuseinroutinebrainsurgery?Surgicalbraininjury(SBI)isaformofbraintraumacausedbyvariousformsofneurosurgicalinterventionsincludingbraintumorexcision,evacuationofintracerebralhemorrhageandbrainlobectomy(e.g.,inepilepsysurgery).CerebraledemaandbrainswellingtypicallyoccurssoonafterSBIandcommonlypeaksonpost-operativedays3to7.SBImaycause

简介：瞄准：调查怎么在老鼠orthotopic肝移植减少胆汁的复杂并发症的发生。方法：165只男Wistar老鼠的一个总数随机被划分成三个组：组A，有修改二手铐的技术的orthotropic肝移植；组B，没有移植，胆汁管被切并且重建；并且组织C，仅仅剖腹术被执行。基于为胆汁的重建使用的途径，组A被划分成二亚群:A1(n=30)，管管重建，和A2(n=30)，管十二指肠重建。在胆汁管复杂并发症上学习动脉重建的影响，组B被划分成四亚群：B1(n=10)，有肝的动脉结扎的管管重建，B2(n=10)，没有肝的动脉结扎的管管重建，B3(n=10)，有肝的动脉结扎的管十二指肠重建，和B4(n=10)，没有肝的动脉结扎的管十二指肠重建。样品被收获在操作以后或在重要胆汁的复杂并发症被发现的时间的14d。结果：在组A，anhepatic阶段是13.7??€?‥吗？？

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简介：Thefutureofingestiblesensorscouldbeacrossbetweensilicon-basedcircuitryandbiodegradablematerials,withbatteriesmadeofnutrientsandrunningonstomachjuices.Ingestiblesensorscouldprovideagutcheckforearlysignsonbacterialinfection,lookforsymptomsofgastrointestinaldisorderssuchasCrohn'sDisease,monitoruptakeofmedications,andevenstudythe

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简介：ScientistsattheUniversityofPittsburghSchoolofMedicinehavediscoveredanewbiologicalpathwayofinnateimmunitythatrampsupinflammationandthenidentifiedagentsthatcanblockit,leadingtoincreasedsurvivalandimprovedlungfunctioninanimalmodelsofpneumonia.TheyreportedtheirfindingstodayinNatureImmunology.

简介：Inthisstudy,Ifocusedonfindingameanofprotectingagainsthearingloss.Byinfusingthecochleawiththeneurotrophinfactor,NT-3aloneorcombinedtreatmentwithMK801,aNMDAreceptorantagonistIfoundhearinglosswasattenuatedandspiralganglionneuronlosswasnearlytotallyprotectedindicatingthattheimportanceofthecombinedtreatmentofNT-3andNMDAreceptorantagonistsinthetreatmentofhearingdisorders.

简介：AbstractHereditary spastic paraplegia type 56 (SPG56-HSP) is a rare autosomal recessive disorder caused by loss of function mutations in CYP2U1, leading to an early-onset limbs spasticity, often complicated by additional neurological or extra-neurological manifestations. Given its low prevalence, the molecular bases underlying SPG56-HSP are still poorly understood, and effective treatment options are still lacking. Recently, through the generation and characterization of the SPG56-HSP mouse model, we were able to take few important steps forward in expanding our knowledge of the molecular background underlying this complex disease. Leveraging the Cyp2u1-/- mouse model we were able to identify several new diagnostics biomarkers (vitamin B2, coenzyme Q, neopterin, and interferon-alpha), as well as to highlight the key role played by the folate pathway in SPG56-HSP pathogenesis, providing a potential treatment option. In this review, we discuss the major role played by the Cyp2u1-/- model in dissecting clinical and biological aspects of the disease, opening the way to a series of new research paths ranging from clinical trials, biomarker testing, and to the expansion of the underlying genetic and molecular, emphasizing how basic mouse model characterization could contribute to advance research in the context of rare disorders.

简介：Itisestimatedthatmorethanonemillionheartfailurehospitalizationsoccureachyear.Systolicheartfailureandheartfailurewithpreservedejectionfractioncontributeequallytoheartfailurehospitalizations.Heartfailurereadmissionratescontinuetobeabout25%.Strategiestoreduceheartfailurereadmissionarekeytoreducinghospitalizationrates.Thestrategiestoreduceheartfailurehospitalizationareasfollows:(1)Duringhospitalization,diuresistotheeuvolemicstateisessential.Fiftypercentofdischargedheartfailurepatientshaveminimalweightlossduringthehospitalization,representingminimaldiuresis,butstillfluidoverload.(2)Duringhospitalization,interrogatethedefibrillatororbiventricularpacemaker(ifapplicable)toensurethatthereisnorightventricularpacingandthereisappropriatebiventricularpacing.Interrogationofdevicescanidentifyarrhythmiaorsuboptimalbiventricularpacing,whichcancontributetodecompensation.(3)Beforedischarge,identifythereasonfordecompensation,suchasatrialfibrillation,infection,pulmonaryembolism,ornoncompliance.(4)Beforedischargeamultidisciplinaryteamisneededtoeducatethepatientondiet,medications,fluidweightsurveillance,andexercise.(5)Apostdischargevisitshouldoccurwithin10daysandwithemphasisonuptitrationofneurohormonalblockersandcontinuedcongestionmanagement.Suchinterventionsconductedbyamultidisciplinaryteamhavethepotentialtoreduceheartfailurehospitalizationrates.

简介：Carbonnanotubescancarryproteinintocellstoinducebiologicaleffects.Amino-functionalizedcarbonnanotubesaresolubleandbiocompatible,havehighreactivityandlowtoxicity,andcanhelppromotenervecellgrowth.Inthisstudy,amino-functionalizedethylenediamine-treatedmulti-walledcarbonnanotubeswereusedtopreparecarbonnanotubes-nervegrowthfactorcomplexesbynon-covalentgrafting.Thephysicochemicalproperties,cytotoxicitytoPC12andchickembryodorsalrootganglion,andbiologicalactivityofthecarbonnanotubes-nervegrowthfactorcomplexeswereinvestigated.Theresultsshowedthataminofunctionalizationimprovedcarbonnanotubes-nervegrowthfactorcomplexdispersibility,reducedtheirtoxicitytoPC12cells,andpromotedPC12celldifferentiationandchickembryodorsalrootganglion.

简介：Todeterminethevalueofdissectingtherecurrentlaryngealnerveduringthyroidsurgerywithrespecttopreventingrecurrentlaryngealnerveinjury,weretrospectivelyanalyzedclinicaldatafrom5344patientsundergoingthyroidectomy.Amongthesecases,548underwentdissectionoftherecurrentlaryngealnerve,while4796didnot.Therewere12casesofrecurrentlaryngealnerveinjuryfollowingrecurrentlaryngealnervedissection(injuryrateof2.2%)and512casesofrecurrentlaryngealnerveinjuryinthosenotundergoingnervedissection(injuryrateof10.7%).Thisdifferenceremainedstatisticallysignificantbetweenthetwogroupsintermsoftypeofthyroiddisease,typeofsurgery,andnumberofsurgeries.Amongthe548casesundergoingrecurrentlaryngealnervedissection,128developedanatomicalvariationsoftherecurrentlaryngealnerve(incidencerateof23.4%),butnorecurrentlaryngealnerveinjurywasfound.Inaddition,theincidenceofrecurrentlaryngealnerveinjurywassignificantlylowerinpatientswiththeinferiorparathyroidglandandmiddlethyroidveinsusedaslandmarksforlocatingtherecurrentlaryngealnervecomparedwiththosewiththeentryoftherecurrentlaryngealnerveintothelarynxasalandmark.Thesefindingsindicatethatanatomicalvariationsoftherecurrentlaryngealnervearecommon,andthatdissectingtherecurrentlaryngealnerveduringthyroidsurgeryisaneffectivemeansofpreventingnerveinjury.

简介：客观CXCR4是为癌症基因治疗的一个潜在的目标。在这研究，指向CXCR4基因的RNA干扰制动火箭病毒向量被构造，并且它对由压抑CXCR4基因表示的前列腺癌症房间的侵略的影响被分析。CXCR4特定的siRNA基因被PCR克隆并且插入了进Pgensil-1原生质标志反对染色U6倡导者和EGFP的方法，然后recombinant碎裂是进PLXSN的亚cloned并且由限制酶测试了并且定序。从transfectedPA317房间获得的病毒是进前列腺癌症房间的transfected。CXCR4mRNA的表示被RT-PCR检测。前列腺癌症房间在试管内的侵略的能力被Transwell实验估计。与recombinantPLXSNtransfection，在前列腺癌症房间PC-3m和LNCaP的CXCR4mRNA的表示以率被减少，这被显示出的结果(84.26±10.20)%并且(88.17±11.23)%分别地。Transwell实验的结果也证明房间在下面的数字微膜分别地，在控制组(P<0.05)与46.9±5.3和66.7±6.0相比在PC-3m和LNCaP的对待的组是14.7±3.1和18.9±4.2。结论PLXSN/EGFP-U6-siCXCR4能显著地压抑表示fCXCR4，前列腺癌症房间在试管内的侵略海角被也禁止。这recombinant碎片将在前列腺癌症的治疗是有用的。