摘要
Objective:Toobservetheeffectsofthermalstressonproliferationofhumanvascularendothelialcells(VECs)andexploreitssignificance.Methods:ChangesofVECsproliferationwereinvestigatedwith3H-TdRincorporationmethodafterECV304wastreatedat43℃for2hours,whileexpressionsofintercellularadhesionmolecule-1(ICAM-1),inhibitorofdifferentiation-1(ID1),andP16andP21proteinsweredeterminedbyWesternBlotting.Results:TheeffectofinhibitionofVECsgrowthafterthermalstresswasdetectedby3H-TdRincorporationexperiment.WesternblottingshowedICAM-1,amarkerofactivatedendothelialcells,wasincreasedmarkedlyafterthermalstress.ExpressionofID1proteindeclinedgraduallywithincreasingexpressionsofitsdownstreamgenes,P16andP21followingthethermalstress.Conclusions:ThermalstresscouldstronglyactivateVECsandinhibitproliferationofVECsthroughID1,thusdownregulatingcyclin-dependentkinaseinhibitors,P16andP21,whichmightbeanessentialpathwayforrecoveryofVECsafterthermalstress.
出版日期
2003年01月11日（中国期刊网平台首次上网日期，不代表论文的发表时间）
