简介：Objective:Tomeasuremovementsofmarkersovertheprimarysiteandassociatedmimicmusclesincertainfacialexpressions,forevaluatingfacialparesisandsynkinesis.Methods:Participantsincluded22normalsubjectsaged45e66years.Maximumshift(Smax)andvelocity(Vmax)weremeasuredusingacustom-designed3-Ddynamicquantitativeanalysissystemoffacialmotion(3-DASFM)basedonmotioncapturetechnology.Measuresweretakenfromperi-oralmusclesduringforcefulbrowraisingandtighteyeclosure,andfrommusclesaroundtheeyeduringgrinning,right/left/bilateralmouthcornerraisingandsmiling.Results:1)Duringforcefulbrowraising,Smaxwas3.65e4.46mmformarkersoverperioralmuscles,withthemarkeroverthenasolabialfoldshowingaVmaxgreaterthanothers(60.60mm/sonleftand62.70mm/sonright).2)Intighteyeclosure,Smaxofperioralmusclemarkerswas1.58e1.92mm,withVmaxbeing11.40e14.76mm/s.3)Ingrinning,thelargesteyemusclemarkerSmaxwasseenatthelowerlid(3.93mmonleftand4.15mmonright)andthesmallestattheinnercanthus(1.59mmonleftand1.53mmonright),withthelargestVmaxseenattheupperlidandsmallestalsoattheinnercanthus(11.71mm/sonleftand11.09mm/sonright).4)Insmiling,thelargestnon-oralSmaxandVmaxwereseenattheupperlid(3.05mmand36.14mm/sonleftand2.53mmand28.90mm/sonright)andthesmallestalsoattheinnercanthus(0.69mmand7.22mm/sonleftand0.77mmand7.80mm/sonright).5)Inrightmouthcornerraising,SmaxandVmaxatlateralandmedialcanthusandatlowerlidweregreateronrightthanleft,whilethoseatupperlidandbrowwereslightlygreateronleftthanright.6)Inleftmouthcornerraising,SmaxandVmaxatlateralcanthusandupperandlowerlidsweregreateronleftthanright.Conclusions:Therearenoabsoluteimmobilepointsonthefacewhenmakingfacialexpressions.Inadditiontotheprimarymovementsite,thereareassociatedmovementsatotherpointsonthefacewithconsistent

简介：Object:ToidentifytranscriptvariantsandexpressionpatternsofporcineMitf.Materialsandmethods:ApairwiseBLASTsearchatNCBIdatabasewasperformedtodeducethestructureofporcineMitfgene.Subsequently,50RACEandfluorescentquantitativeRT-PCRwereusedtoanalyzetheexpressionpatternofporcineMitfindifferenttissues.Results:FourtranscriptvariantsofporcineMitf,MITF-A,MITF-H,MITF-MandMITF-SUSwereidentified,allsharinghighhomologywiththoseinhumans,exceptMitf-SUS.Conclusion:ThesequenceofporcineMitfappearhighlyhomologoustohumanMITF.However,only4transcriptvariantsofporcineMitfwereidentifiedintheseminipigs,lessthanthe9transcriptvariantsinhumanMITF.

简介：BackgroundPregnancycomplicatingpulmonaryarterialhypertension(PAH)isalife-threateningobstetricalcomplication.FewstudieshavereportedtheclinicalcharacteristicsandoutcomesofpregnantwomenwithPAH.MethodsRetrospectivelyanalysiswasconductedon86casesofpregnantwomenwithPAHattheDepartmentofObstetricinGuangdongGeneralHospitalbetween2009and2014.Dataincludedpatients'age,theetiologiesofPAH,clinicalclassificationoftheNewYorkHeartAssociation(NYHA),gestationalweeks,deliverymode,maternalandfetaloutcomes.Results1Therewere2casesofidiopathicpulmonaryarterialhypertension(IPAH)and84casesofsecondarypulmonaryarterialhypertension.Thelatterwascomposedby40(47.61%)casesofcongenitalheartdisease(CHD),38(45.23%)casesofrheumaticheartdisease(RHD),3(3.57%)casesofsystemiclupuserythematosus(SLE)and1(1.19%)caseofpartialhydatidiformmole.ThenumberofpatientsclassifiedinNYHAI~IVwere8(9.30%),41(47.67%),26(30.23%)and11(12.7%),respectively.Andthemortalitywas3.48%(3/86).2Amongthe86patients,31(36.04%)womengavebirthattermand36(41.86%)hadprematuredelivery.Inaddition,16(18.6%)womenhadanabortionlessthan28weeks.Therewereatotalof65(75.58%)livebirths,61(93.84%)ofwhomweredeliveredviacesareansection,whiletheother4(6.15%)werevaginaldeliveries.3Patientsweredividedinto3groups,12ofmild(<40mmHg),35ofmoderate(40~70mmHg)and39ofsevere(≥70mmHg)regardingthepulmonaryarterysystolicpressure(sPAP)measuredbyultrasound.WedefinedtheformertwoasgroupAandthelatterasgroupB.Thesignificancewasseenstatisticallyingestationalweeks(32.86±8.86gvs28.76±9.84),therateoftransferringtoICU(19.14%vs53.84%),neonatalbirth-weight(2725.69±467.43vs2044.82±665.75g),APGARscoring(9.97±0.16vs8.68±2.08),maternalstatusofheartfunctionandadversepregnantoutcomes,whencomparedgroupAwithB.AlltheP-valuesmentionedabovew

简介：AIM:Toreviewpublishedclinicalstudiesexaminingtheeffectofnatamycininthetreatmentoffungalkeratitis.METHODS:WeselectedthepublicationsinCENTRAL,MEDLINE,EMBASE,CNKI,andCBM.Thisstudysystematicallyreviewedpublishedrandomizedcontrolledtrials(RCTs)thatcomparednatamycintootherantifungalagents,andconductedfeasibleMeta-analysisofefficacyresultsusingRevman5.2software.RESULTS:WeincludedseventrialswhichweremainlycarriedoutindevelopingcountriesofAsia,withfivetrialsconductedinIndia,oneeachinChinaandBangladesh.Atotalof804participantswererandomizedtofollowingcomparisons:2%econazoleversus5%natamycinshowedlittledifferenceintheeffectsoftreatmentoffungalkeratitis[RR=0.99,95%confidenceinterval(CI),0.8to1.21];chlorhexidinegluconateversus5%natamycinindicatedthattheresultsonhealingoftheulcerat21dwaslessconclusive(RR=0.77,95%CI,0.55to1.08;I2=0%);1%voriconazoleversus5%natamycinsuggestedthatnatamycintreatmentappearedtobesignificantlybetteroutcomesthanvoriconazole(regressioncoefficient=-0.18logMAR;95%CI,-0.30to-0.05;P=0.006),especiallyinFusariumcases(regressioncoefficient=-0.41logMAR;95%CI,-0.61to-0.20;P<0.001);natamycinversusfluconazoleshowedasignificantdifferenceincurerate(χ2=5.048,P<0.05)andnatamycingroupwasmoreeffectivethanfluconazoleinaverageperiodoftherapy(t=7.94,P<0.01).CONCLUSION:Natamycinwasapreferablechoiceinthetreatmentoffungalkeratitis,especiallyintheearlyperiodofFusariumcases.

简介：

简介：PlatycodinD(PD)，从Platycodonis根值孤立的triterpenoidsaponin，是被显示了在几根癌症房间线有anti-proliferative效果的一副著名中草药。这研究的目的是用proteomics途径与PD在hepatocellular癌HepG2房间的治疗以后在细胞的蛋白质决定变化。房间生存能力用MTT试金被决定。proteome被二维的差别胶化电气泳动和帮助矩阵的激光解吸附作用/电离time-of-flight团spectrometry分析。西方的污点分析被用来证实改变的蛋白质的表示。我们的结果证明PD在集中依赖者和时间依赖者礼貌禁止了HepG2房间的增长。十六蛋白质被识别在对待PD的HepG2房间起来调整包括ATP5HOXCT1，KRT9，CCDC40，ERP29，RCN1，ZNF175，HNRNPH1，HSP27，PA2G4，哲学学士，BANF1，TPM3，ECH1，LGALS1，和MYL6三蛋白质(即，RPS12，EMG1，和KRT1)与PD在治疗以后在HepG2房间减少了。在HSP27和哲学学士的变化被西方的弄污进一步证实。在结论，我们的结果为PD的反癌症活动在行动的机制上打开新灯。

简介：AIMTodescribeathree-dimensionalmodel（3DM）toaccuratelyreconstructanatomicrelationshipsofcentrallylocatedhepatocellularcarcinomas（HCCs）.METHODS：FromMarch2013toJuly2014,reconstructionsandvisualsimulationsofcentrallylocatedHCCswereperformedin39patientsusinga3Dsubject-basedcomputedtomography（CT）modelwithcustomdevelopedsoftware.CTimageswereusedforthe3DreconstructionofCouinaud＇spediclesandhepaticveins,andthecalculationofcorrespondingtumorterritoriesandhepaticsegmentswasperformedusingYorktalDMITsoftware.Therespectivevolume,surgicalmargin,andsimulatedvirtualresectionoftumorswerealsoestimatedbythismodelpreoperatively.Allpatientsweretreatedsurgicallyandtheresultswereretrospectivelyassessed.Clinicalcharacteristics,imagingdata,procedurevariables,pathologicfeatures,andpostoperativedatawererecordedandcomparedtodeterminethereliabilityofthemodel.RESULTS：3Dreconstructionallowedstereoscopicidentificationofthespatialrelationshipsbetweenphysiologicandpathologicstructures,andofferedquantifiableliverresectionproposalsbasedonindividualizedliveranatomy.Thepredictedvalueswereconsistentwiththeactualvaluesfortumormassvolume（82.4±109.1mLvs84.1±108.9mL,P=0.910）,surgicalmargin（10.1±6.2mmvs9.1±5.9mm,P=0.488）,andmaximumtumordiameterthenumberandextentofportalvenousramifications,aswellastheirrelationtohepaticveins,werevisualized.Preoperativeplanningbasedonsimulatedresectionfacilitatedcompleteresectionoflargetumorslocatedintheconfluenceofmajorvessels.Andmostofthepredicteddatawerecorrelatedwithintraoperativefindings.CONCLUSION：This3DMprovidesquantitativemorphometryoftumormassesandastereo-relationshipwithadjacentstructures,thusprovidingapromisingtechniqueforthemanagementofcentrallylocatedHCCs.

简介：Redbloodcells(RBC)’flickeringpresentthedynamicpropertiesofthecytomembrane.Itscomplexitycouldbeusedforaginganalysisortheevaluationforthestoragequality.Theflickeringactivityisakindofreversibleperpendicularmotionofthespecifiedpixel.Therefore,thecomplexityanalysisdependsonthereliabledetectionoftemporalvariationforthegray-scalevaluesfromeachpixelofthecells.Inthispaper,weimprovedourpreviousworkonthescreeningofthehorizontaldriftedcellswithasurfacebasedoncellregistrationmethodandtheeffectofGSMexposuretothedynamicpropertiesoftheRBCsintermsofmulti-scalesampleentropywaspresentedinthepaper.

简介：Objective:Hepatocellularcarcinoma(HCC)isaleadingcauseofcancer-relateddeaths.NovelserumbiomarkersarerequiredtoincreasethesensitivityandspecificityofserumscreeningforearlyHCCdiagnosis.ThisstudyemployedaquantitativeproteomicstrategytoanalyzethedifferentialexpressionofserumglycoproteinsbetweenHCCandnormalcontrolserumsamples.Methods:Lectinaffinitychromatography(LAC)wasusedtoenrichglycoproteinsfromtheserumsamples.Quantitativemassspectrometricanalysiscombinedwithstableisotopedimethyllabelingand2Dliquidchromatography(LC)separationswereperformedtoexaminethedifferentiallevelsofthedetectedproteinsbetweenHCCandcontrolserumsamples.Westernblotwasusedtoanalyzethedifferentialexpressionlevelsofthethreeserumproteins.Results:Atotalof2,280proteingroupswereidentifiedintheserumsamplesfromHCCpatientsbyusingthe2DLC-MS/MSmethod.Upto36proteinswereup-regulatedintheHCCserum,whereas19proteinsweredown-regulated.Threedifferentialglycoproteins,namely,fibrinogengammachain(FGG),FOS-likeantigen2(FOSL2),andα-1,6-mannosylglycoprotein6-β-N-acetylglucosaminyltransferaseB(MGAT5B)werevalidatedbyWesternblot.Allthesethreeproteinswereup-regulatedintheHCCserumsamples.Conclusion:AquantitativeglycoproteomicmethodwasestablishedandprovenusefultodeterminepotentialnovelbiomarkersforHCC.

简介：Xingxiong注射(XXI)是叶片白果树摘录和ligustrazine为心血管、脑血管的疾病的治疗作好准备的一个广泛地使用的中国草药的公式。与药理学研究相比，这个公式上的化学分析和质量控制研究是相对有限的。在现在的学习，高效液体层析结合了四极time-of-flight团spectrometry(HPLC-QTOFMS)方法在XXI被用于成分的全面分析。根据破碎规则和以前的报告，三十白果树flavonoids，四白果树萜烯内酯，和一碱被识别。高效液体层析结合了三倍的四极团spectrometry(HPLC-QQQMS)方法然后被使用确定在XXI的十主要成分。方法确认结果显示发达方法有理想的特性，线性，精确和精确性。白果树flavonoids的全部的内容关于22.05-25.51g·mL−1，白果树萜烯内酯数量分别地关于在XXI样品的六批的4.41-8.70g·mL−1。而且，余弦比率算法和距离大小被采用评估XXI样品的类似，并且结果表明了高质量的一致性。这个工作能在Xingxiong注射的质量控制上提供全面信息，它在一个合理质量控制标准的建立是有用的。

简介：BackgroundPrematureventricularcontractions(PVCs)arefrequentlyseeninchildren.However,therearelesssystematicandlongerfollowing-upstudiesexaminingtheprognosisofPVCsinchildren.TheaimofthisstudywastoevaluatethemediumtolongtermprognosisofPVCsinchildhoodandwhetherthereisadifferentialprognosisindifferentprimarydiseasesofPVCs.MethodsThisstudyreviewedthedataof106pediatricpatients(49F/57M,7.5±3.8years)seenattheAffiliatedHospitalofQingdaoUniversitywiththediagnosisofPVCsbetween1999and2005.Dataondemographics,clinicalpresentation,laboratorytests,andechocardiogramsofpatientswereextractedfromtheavailableclinicalrecords.ResultsAtotalof35(33.0%)childrenpresentedwithPVCsduetomyocarditis,7(6.6%)duetocardiomyopathies,7(6.6%)duetomitralvalveprolapse(MVP),10(9.4%)duetooperationforcongenitalheartdisease(O-CHD),16(15.2%)duetoleftventricularfalsetendons(LVFT),and31(29.2%)duetounknowncause.HolterdidnotshowPVCsduringfollow-upperiodin100%ofmyocarditispatients,57%ofcardiomyopathypatients,71%ofMVPpatients,60%ofO-CHDpatients,88%ofLVFTpatients,87%ofunknowncausepatients.ThePVCsdisappearedin93%ofpatientswhodidnotuseanti-arrhythmicdrugsandin76%ofpatientswhousedantiarrhythmicdrugs.Therewasnoasignificantdifferenceinprognosisbetweenmyocardialnutritioncombinedwithintravenousinjectionofimmunoglobulin(IVIG)groupandpropafenonegroup.ConclusionsPVCscausedbydifferentprimarydiseaseshasafavorableprognosisinchildren.Usually,thePVCswillreduceevendisappearduringfollow-up.ThepatientswithPVCsduetomyocarditisshouldbepreferredusemyocardialnutrientcombinedwithIVIG.

简介：AIM:Tocomparetheeffectsofintravitrealinjectionofbevacizumab(IVB)withintravitrealtriamcinoloneacetonide(IVTA)onthetreatmentofcystoidmacularedema(CME)secondarytoretinalveinocclusion(RVO).·METHODS:AliteraturesearchwasconductedusingPubMed,theCochraneCentralRegisterofControlledTrials,WebofScienceandtheChineseBiomedicalDatabase.Thecomparisonwasdividedintotwogroups,group1conductedcomparisoninbranchRVO(BRVO)orcentralRVO(CRVO),group2conductedcomparisoninischemic-RVOornonischemic-RVO.Pooledmeandifferences(MDs)forchangesinvisualacuity(VA),centralmacularthickness(CMT)andintraocularpressure(IOP)werecalculatedingroupsat4,12and24wkaftertreatmentrespectively.·RESULTS:EightstudiescomparingtheefficacyofIVBwithIVTAwereincludedintheMeta-analysis.Ingroup1,inBRVO,significantdifferencewasshownonthecomparisonofCMTat24wk(MD,-45.66;95%CI,-76.03to-15.28;P=0.003),IVBwaseffectiveonBRVOforatleast24wk;nosignificantdifferenceswerefoundinthecomparisonofVAateachtimepoints(P>0.05respectively).InCRVO,nosignificantdifferenceswerefoundinthecomparisonofVAorCMTbetweenIVBandIVTAateachtimepoints(P>0.05,respectively).Ingroup2,inischemic-RVO,significantdifferenceswereshowninthecomparisonofVA(MD,-0.28;95%CI,-0.42to-0.14;P<0.0001)andCMT(MD,-86.50;95%CI,-151.18to-22.43;P=0.008)at24wk;Innonischemic-RVO,nosignificantdifferencesweredemonstratedinthecomparisonofVAorCMTbetweenIVBandIVTAateachtimepoints(P>0.05,respectively).TheoccurrenceofhighIOPwasmuchlowerinIVBgroup.·CONCLUSION:ThisMeta-analysissuggestedthatIVBwaseffectiveindecreasingCMTinBRVOforatleast24wk,IVBismoreeffectiveonimprovingVAandreducingCMTinischemic-RVO.IVBismorepromisingonRVOthanIVTA.

简介：维生素D受体(VDR)多型性作为潜在的贡献者被学习了到多重硬化(MS)。然而，出版了研究关于学习设计和检测的效果的意义不同。这研究的目的是确定与在用元分析的MS的VDR多型性接近的TaqI，BsmI，ApaI和FokI联系的风险的大小。为系统的评论和元分析指南跟随比较喜欢的报导项目，我们进行了文学的系统的搜索和元分析。亚群分析被执行从选择学习特征检测异质的潜在的来源。概括风险的稳定性用敏感分析被评估。元分析从13盒子控制研究包括了3300个案例和3194控制的一个总数。没有重要协会，在TaqI和BsmI多型性和MS风险之间发现。在ApaI多型性和MS风险之间的协会是重要的在同型结合并且codominant模型(P=；0.013并且P=；0.031，分别地)，建议AAApaI遗传型可能是一个重要MS风险因素。出版年和年龄显著地影响了在TaqI多型性和MS之间的协会(P=；0.014并且P=；0.010，分别地)，它显示主要T等位基因的保护的效果。AAApaI和FFFokI遗传型是为MS的重要风险因素。在TaqI多型性和MS风险之间的协会被学习特征显著地影响。

简介：Thispaperaimstotheresearchoftheimpactoffluidshearstressontheadhesionbetweenvascularendothelialcellsandleukocyteinducedbytumornecrosisfactor-α(TNF-α)bymicrofliudicchiptechnology.Microfluidicchipwasfabricatedbysoftlithograph;Endothelialmicrofluidicchipwasconstructedbyoptimizingtypesoftheextracellularmatrixproteinsmodifiedinthemicrochannelandcellincubationtime;humanumbilicalveinendothelialcellsEA.Hy926linedinthemicrochannelwereexposedtofluidshearstressof1.68dynes/cm~2and8.4dynes/cm~2respectively.Meanwhile,adhesionbetweenEA.Hy926cellsandleukocytewasinducedbyTNF-αunderaflowcondition.EA.Hy926cellculturedinthestaticconditionwasusedascontrolgroup.Thenumbersoffluorescently-labeledleukocyteinmicrochannelwerecountedtoquantizetheadhesionlevelbetweenEA.Hy926cellsandleukocyte;cellimmunofluorescencetechniquewasusedtodetecttheintercellularadhesionmolecule(ICAM-1)expression.TheconstructedendothelialmicrofluidicchipcanaffordtothefluidshearstressandrespondtoexogenousstimulusofTNF-α;comparedwiththeadhesionnumbersofleukocyteincontrolgroup,adhesionbetweenEA.Hy926cellsexposedtolowfluidshearstressandleukocytewasreducedunderthestimulusofTNF-αataconcentrationof10ng/ml(P<0.05);leukocyteadhesionwithEA.Hy926cellsexposedtohighfluidshearstresswasreducedsignificantlythanEA.Hy926cellsincontrolgroupandEA.1Hy926cellsexposedtolowfluidshearstress(P<0.01);theregulationmechanismoffluidshearstresstotheadhesionbetweenEA.Hy926cellsandleukocyteinducedbyTNF-αwasthroughthewayofICAM-1.Theendothelialmicrofluidicchipfabricatedinthispapercouldbeusedtostudythefunctionsofendothelialcellinvitroandprovideanewtechnicalplatformforexploringthepathophysiologyoftherelatedcardiovascularsystemdiseasesunderaflowenvironment.

简介：Objective:Tocomparetheefficacyandadverseeffectsofpaclitaxel-etoposide-carboplatin/cisplatin(TEP/TCE)regimenwiththoseofetoposide-carboplatin/cisplatin(EP/CE)regimenasfirst-linetreatmentforcombinedsmall-celllungcancer(CSCLC).Methods:Aretrospectivestudywasconductedon62CSCLCpatientswhoweretreatedatTianjinMedicalUniversityCancerInstituteandHospitalfromJuly2000toApril2013andadministeredwithTEP/TCEregimen(n=19)orEP/CEregimen(n=43)asfirst-lineCSCLCtreatment.Allpatientsreceivedmorethantwocyclesofchemotherapy,andtheresponsewasevaluatedeverytwocycles.Theprimaryendpointwasoverallsurvival(OS),andthesecondaryendpointswereprogression-freesurvival(PFS),objectiveresponserate(ORR),diseasecontrolrate(DCR),andadverseeffects.Results:ORRbetweentheTEP/TCEandEP/CEgroupsshowedastatisticaldifference(90%vs.53%,P=0.033).BothgroupsfailedtoreachastatisticaldifferenceinDCR(100%vs.86%,P=0.212).ThemedianPFSandOSoftheTEP/TCEgroupwereslightlylongerthanthoseoftheEP/CEgroup,althoughbothgroupsfailedtoreachastatisticaldifference(10.5vs.8.9months,P=0.484;24.0vs.17.5months,P=0.457).However,stratifiedanalysisindicatedthatthePFSofpatientswithstagesIIIandIVCSCLCshowedmarginallysignificantdifferencebetweentheTEP/TCEandEP/CEgroups(19.5vs.7.6months;P=0.071).BothratesofgradeIVbonemarrowdepressionandterminationofchemotherapyintheTEP/TCEgroupweresignificantlyhigherthanthoseintheEP/CEgroup(26.3%vs.7.0%,P=0.036;31.6%vs.14.7%,P=0.004).Conclusion:TheTEP/TCEregimenmaynotbepreferredforCSCLC,andthisthree-drugregimenrequiresfurtherexplorationandresearch.Todate,theEP/CEregimenremainsthestandardtreatmentforCSCLCpatients.

简介：Objective:Ameta-analysiswasperformedtoaugmenttheinsufficientdataontheimpactofmutativeEGFRdownstreamphosphatidylinositol-3-kinase(PI3K)andmitogen-activatedproteinkinase(MAPK)pathwaysontheclinicalefficiencyofepidermalgrowthfactorreceptortyrosinekinaseinhibitor(EGFR-TKI)treatmentofnon-smallcelllungcancer(NSCLC)patients.Methods:NetworkdatabaseswereexploredinApril,2015.PapersthatinvestigatedtheclinicaloutcomesofNSCLCpatientstreatedwithEGFR-TKIsaccordingtothestatusofK-rasand/orPIK3CAgenemutationwereincluded.Aquantitativemeta-analysiswasconductedusingstandardstatisticalmethods.Oddsratios(ORs)forobjectiveresponserate(ORR)andhazardratios(HRs)forprogression-freesurvival(PFS)andoverallsurvival(OS)werecalculated.Results:MutationinK-rassignificantlypredictedpoorORR[OR=0.22;95%confidenceinterval(CI),0.13-0.35],shorterPFS(HR=1.56;95%CI,1.27-1.92),andshorterOS(HR=1.59;95%CI,1.33-1.91)inNSCLCpatientstreatedwithEGFR-TKIs.MutantPIK3CAsignificantlypredictedshorterOS(HR=1.83;95%CI,1.05-3.20),showedpoorORR(OR=0.70;95%CI,0.22-2.18),andshorterPFS(HR=1.79;95%CI,0.91-3.53)inNSCLCpatientstreatedwithEGFR-TKIs.Conclusion:K-rasmutationadverselyaffectedtheclinicalresponseandsurvivalofNSCLCpatientstreatedwithEGFRTKIs.PIK3CAmutationshowedsimilartrends.InadditiontoEGFR,addingK-rasandPIK3CAasroutinegenebiomarkersinclinicalgeneticanalysisisvaluabletooptimizetheeffectivenessofEGFR-TKIregimensandidentifyoptimalpatientswhowillbenefitfromEGFR-TKItreatment.

简介：摘要目的分析住院患者取消手术原因，以提高手术床使用率，缩短平均住院日，减少住院费用。方法回顾性分析我院外科系统2014年5月-2015年4月所有住院患者中取消手术发生的频率及原因。结果2729台择期手术中取消153台（5.6%），原因主要涉及疾病因素（66%）、患者因素（24.2%）、手术医生因素（9.8%）。结论所有取消择期手术中，大多数可以通过相应整改措施加以避免，取消手术率有很大下降空间。

简介：摘要目的对于儿科护理中存在的纠纷进行原因分析，进而对于护理进行干预措施分析。方法通过对照组和观察组相结合的方式，选取医院2014年下半年的儿科护理案例100例来进行分析，通过分层的方式，50人为对照组，50人为观察组，其中对照组采取常规性的护理方式，而观察组则采取全面性的护理干预。结果通过两个组的儿科护理治疗效果的对比分析，观察组通过全面性的护理干预方式，其纠纷的发生率明显降低，而其满意度则是不断的提高，都优于对照组。结论通过100例儿科护理的干预措施，通过全面向的护理，对于纠纷发生率降低具有十分重要的作用，对于儿科护理具有十分重要的影响，需要进行不断的推广。

简介：摘要随着社会发展，人们的健康及维权意识增强，儿科护理方面潜在的不安全因素日益增多，给护理人员带来很大的工作压力。为了满足新形势下对于儿科护理方面的更高要求，本文总结了儿科护理工作中存在的缺陷进行分析，据此探求有效的护理管理措施。

简介：摘要目的为了规范护理记录，避免和减少因护理记录缺陷而引起医患纠纷。方法采取随机抽查1120份归档病历和现病历，通过对目前护理记录存在的语言不规范、记录呈流水帐式、记录不全面等进行回顾性分析。结果了解护理记录中易出现的书写缺陷，熟悉避免缺陷的应对措施，有利于护士不断规范自己的行为，提高护理质量，为正确施治、施护提供有力的书面证据。结论应重视护理记录，促进护理工作不断改进。