简介:随着我国经济社会的发展和桥梁科学技术的进步,大跨度钢拱桥得到飞速发展。作为钢拱桥的关键构件,H型刚性吊杆的稳定性对钢拱桥的安全运行发挥着至关重要的作用,因其长细比大、阻尼低的特点,在风荷载作用下易产生大幅振动。风致扭转振动作用下H型刚性吊杆的疲劳问题受众多因素影响,且现有振动控制目标不够精确,疲劳评估方法有待完善。因此,开展H型刚性吊杆的疲劳评估研究并建立优化设计流程具有重要的现实指导意义。
简介:BackgroundTaxifolin(Tax)isanessentialnaturalantioxidant.MultiplestudieshaveshownthatTaxcanprotectcardiomyocytesfromischemia-reperfusioninjury.However,theunderlyingmechanismisstillunclear.MethodsH9C2cellswererandomlydividedintocontrol,H_2O_2group,Taxpretreatmentgroup(Tax+H_2O_2);Taxeffectgroup.CellactivitywasdetectedbyCCK-8andtheintracellularstructurewasobservedbytransmissionelectronmicroscopy.AutophagywasdeterminebyWesternblottinganalysisofBeclin-1,Bcl-2andPKC.ResultsTaxpretreatmentsignificantlyincreasedanti-apoptoticproteinBcl-2andautophagyproteinBeclin-1.ExpressionofPKCwasinhibitedbyTax.ConclusionsTaxpretreatmentcouldprotectH9C2cellsagainstH_2O_2-induceddamagethroughtheBcl-2andautophagypathways.