简介：SulfatecanbeactivatedbyATPsulfurylaseandadenosine5-phosphosulfatekinase(APSK)invivo.RecentstudiessuggestedthatAPSKinArabidopsisthalianaregulatedthepartitionbetweenAPSreductionandphosphorylationanditsactivitycanbemodulatedbycellularredoxstatus.InordertostudyregulationofAPSKinrice(OsAPSK),OsAPSKIgenewasclonedanditsactivitywasanalyzed.OsAPSKIC36andC69werefoundtobetheconservedcounterpartsofC86andC119,whichinvolvedindisulfideformationinAtAPSK.C36A/C69AOsAPSK1doublemutationwasmadebysitedirectedmutagenesis.OsAPSKanditsmutantwereprokaryoticallyover-expressedandpurified,andthenassayedforAPSphosphorylationactivity.OsAPSK1activitywasdepressedbyoxidizedglutathione,whiletheactivityofitsmutantwasnot.Furtherstudiesinthecasethatoxidativestresswillfluctuateinvivo3'-phosphoadenosine-5'-phosphosulfatecontent,andallAPSKisoenzymeshavesimilarregulationpatternsarenecessarytobeperformed.

简介：一个水耕法的文化实验被做在谷胱甘肽内容(GSH)和谷胱甘肽S-transferase上调查Cd应力的效果(GST，EC2.5.1.18)在稻秧的活动。当在答案的Cd水平比10mg/L高时，米饭生长严重地被禁止。在米饭射击，，GSH内容和GST活动在根与增加的Cd水平增加了，GST被Cd处理显然禁止。与射击相比，米饭根有更高的GSH内容和GST活动，显示Cd解毒的能力在比在射击的根是高得多的。在Cd水平和GSH内容或GST活动之间有重要关联，建议两个参数可以被用作在米饭的Cd应力的简历标记。

简介：Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.

简介：四十个SSR标记被用来在在1950年代并且在种的151个中国主要米饭变化比较基因差异变化最近十年。40SSRloci，39被发现当时，多态一地点(RM479)monomorphic。213等位基因的一个总数从39多态的loci被识别。等位基因的平均数字每地点(Na)具有5.5，从2～11。Nei的基因差异索引(他)在RM418从0.309atRM174在loci之中急速地变化了到0.869，与0.649的平均值。在在indica和装饰用的梨树亚种之间的SSRallelic差异的在那里存在的重要差别，和indica在Na两个都比装饰用的梨树有更多的变化并且他。由有在Na的基因变化的比较并且他，在1950年代种的变化有更多的等位基因，这被揭示并且更高他比那些在里面最近为indica的十年和装饰用的梨树米饭。为Na的二亚种之间的差别随着时间的过去在一个趋势是重要的(indica：z=2.677，P=0.007；装饰用的梨树：z=3.441，P=0.001)，然而并非为重要他(indica：z=1.471，P=0.141；装饰用的梨树：z=1.932，P=0.053)。分子的变化(AMOVA)的分析显示了那在那里存在的重要差别(P<0.05)在在二个时期，更多的遗传变异被indica(Fst=0.050)和装饰用的梨树贡献之间的遗传变异(Fst=0.082)子集。用locus-by-locusAMOVA过程，重要基因区别为indica变化和11loci在13loci(RM21，RM128，RM147，RM169，RM190，RM221，RM231，RM251，RM253，RM317，RM341，RM418，和RM478)被观察(RM101，RM135，RM152，RM159，RM169RM190RM251RM253RM311，RM418，和RM478)为在二时期之间的装饰用的梨树一。Itwas发现一些等位基因作为与那些in1950s作比较沉醉于当前的主要米饭变化。因此，利用更多的相异的精英应该是必要的为在当前的米饭繁殖的遗传背景的扩展的基因资源编程序。

简介：Twonewlybredhybridricecombinations,superhigh-yieldingLiangyoupeijiu(Pei'ai64S×9311)andPei'ai64S/E32(Pei'ai64S×E32)wereusedtoinvestigatethephotosyntheticcharacteristicsunderhightemperatureincomparisonwithhybridriceShangyou63.Hightemperaturecausedadecreasedphotosyntheticefficiencyandaggravatedphotoinhibition.TheoptimumtemperatureforphotosyntheticelectrontransportationandphotosyntheticCO2fixationwereabout28℃and35-40℃respectively.Linearelectrontransportationismoresensitivetohightemperaturethanthephotochemicalprocess.Themechanismofhightemperatureadaptationwaspossiblyasfollows:superhigh-yieldingricehasquicklyincreasingcarotenoid,whichactedasamorefavorableantioxidantsystemtoreducetheactiveoxygenproductionandavoiddamagetothephotosynthesissystem;superhigh-yieldingricehasahigherefficiencyofxanthophyllscycletodissipateexcessheatenergy;superhigh-yieldingricehasamorestablephotosyntheticfunction,higherphotosyntheticefficiencyandmoreheatstableproteincontent.

简介：布朗planthopper(Nilaparvatalugens圣?l)大多数损坏害虫之一在米饭正在引起hopper灼伤，并且从而减少生产率并且另外产品的质量。控制这个害虫的有效管理策略是到本地米饭栽培变种的理想的基因的鉴定和转移。为开发抵抗栽培变种的最重要的途径是标记的鉴定，它能在更持久的抵抗遗传型的帮助标记的选择帮助。易受影响的父母IR50和抵抗父母Ptb33，和他们的F2人口是为有随机的放大多态的DNA的抵抗基因的鉴定的使用的inbulkedsegregant分析标记(RAPD)教材。教材OPC7和OPAG14证明主导、易受影响的特定的banding模式那么叫了co主导的标记。而且，OPC7697和OPAG14680给抵抗特定的乐队看了并且因此在联合分阶段执行，而OPC7846和OPAG14650给易受影响的特定的genotypic乐队看了inbulkedsegregant分析。因此，联合阶段标记，OPC7697和OPAG14680，被认为在在庄稼改进的米饭遗传型的帮助标记的选择更有用。

简介：Precisebaseeditingishighlydesiredinplantfunctionalgenomicresearchandcropmolecularbreeding.Inthisstudy,weconstructedarice-codonoptimizedadeninebaseeditor(ABE)-nCas9toolthatinducedtargetedA·TtoG·Cpointmutationofakeysinglenucleotidepolymorphismsiteinanimportantagriculturalgene.Combinedwiththemodifiedsingle-guideRNAvariant,ourplantABEtoolcanefficientlyachieveadeninebaseeditinginthericegenome.

简介：Homeoboxtranscriptionfactorsparticipateinthegrowthanddevelopmentofplantsbyregulatingcelldifferentiation,morphogenesisandenvironmentalsignalresponse.Torevealthefunctionsofthesetranscriptionfactorsinrice,weconstructedtheRNAivectorsofOsHox9,amemberofhomeoboxfamily,andanalyzedthefunctionofOsHox9usingreversegenetics.TheplantheightandtilleringnumberofRNAitransgenicplantsdecreasedcomparedwiththoseofwild-typeplants.Reversetranscription-polymerasechainreactionanalysisshowedthatOsHox9expressionreducedinthetransgenicplantswithphenotypicvariance,whereasthatinthetransgenicplantswithoutphenotypicvariancewassimilartothatinthewild-typeplants.ThisresultsuggeststhatthephenotypesofthetransgenicplantswerecausedbyRNAieffects.Thetissue-specificityofOsHox9expressionindicatedthatitwasexpressedindifferentorgans,withhighexpressioninstemapicalmeristemandyoungpanicles.SubcellularlocationofOsHox9demonstratedthatitwaslocalizedonthecellmembrane.

简介：EliminationoftheCRISPR/Cas9constructsineditedplantsisaprerequisiteforassessinggeneticstability,conductingphenotypiccharacterization,andapplyingforcommercializationoftheplants.However,removaloftheCRISPR/Cas9transgenesbygeneticsegregationandbybackcrossislaboriousandtimeconsuming.WepreviouslyreportedthedevelopmentofthetransgenekillerCRISPR(TKC)technologythatusesapairofsuicidegenestotriggerself-eliminationofthetransgeneswithoutcompromisinggeneeditingefficiency.TheTKCtechnologyenablesisolationoftransgene-freeCRISPR-editedplantswithinasinglegeneration,greatlyacceleratingcropimprovements.Here,wepresentedtwonewTKCvectorsthatshowgreatefficiencyinbotheditingthetargetgeneandinundergoingself-eliminationofthetransgenes.ThenewvectorsreplacedtheCaMV35SpromoterusedinourpreviousTKCvectorwithtworicepromoterstodriveoneofthesuicidegenes,providingadvantagesoverourpreviousTKCvectorundercertainconditions.Thevectorsreportedhereofferedmoreoptionsandflexibilitytoconductgeneeditingexperimentsinrice.

简介：RiceisanimportantfoodcropinChina,andthedevelopmentofhybridriceisacrucialwaytoincreasegrainyield.Thecreationofdual-purposenuclear-sterilelinesfortwo-linehybridbreedinghasbecomevitalforcommercialricebreeding.WeconstructedthepC1300-2x35S::Cas9-sgRNAPTGMS2-1expressionvectorforeditingthemalefertilitygenePTGMS2-1intwowidelycompatiblericevarieties,93-11andHuazhan,byusingtheCRISPR/Cas9system.Weobtainedthemarker-freephotoperiod-/thermo-sensitivegenicmale-sterile(P/TGMS)linesinT1generation.Accordingtotheexperimentsinphytotronwithfourtemperatureandphotoperiodtreatments,wefoundthetemperatureisthemainfactorforrestoringthepollenfertilityofptgms2-1mutantsin93-11andHuazhan,andthephotoperiodalsohassomeeffectsonpollenfertilityintwodifferentricebackgrounds.Theapplicationofcultivatingnewmale-sterilelinesbygenomeeditingsystemwillsignificantlyacceleratethericebreedingprocess.