简介：IntroductionSomewritingisgoodinsentencestructurebuteitherpoorincoherenceorincohesion.Theproblemofcoherenceisthatthesequenceofinformationisnotalwaysclearandtheproblemofcohesionisthatthesequenceofinformationisacceptable,whiletheconnectionbetweenitisnotsogood.Thequestionishowtoanalyzeandimprovethesedifferentweaknessinwriting.Wehavetwomethods.Onetofocusoncoherence,theothertofocusoncohesion.Inthisessay,themethodusedisbasedontheprinciplethatcoherenceandcohesionshouldworktogether.Iusethisapproachasatooltoeditanddisplaytheanalysisofcoherenceandcohesioninthepassageentitled’Father&Son".ThispassageispresentedasaguidedwritingexerciseinCollegeEnglishCourse,IntensiveReading,Band3,p271.

简介：Wepresentamethodtorepresentmulti-resolutionvectorgraphicssuchasroadnetworksorrailwaynetworksinvirtualenvironment.Thesevectordatacanbeinteractivelyeditedandthelandscapeandbeexploredinrealtimeatanyaltitudefromflightviewtocarview.Wedesignacontext-focusedvectordescriptionoflinearcanarealfeatures,withassociatedcustomizeddefinitionpaintertospecifytheirappearance(colorandmaterial)andtheirdisplaymode(detailedmodeorsimplifiedmode).Therearesomespecialproblemsindrawingvectorgraphicsinvirtualenvironment.Floating-pointround-offerrorappearwhenweusealowviewpointtoobservethescene,anditleadstoscenejittering.Drawing3Dwidelinesturnsintoaproblemon3Dterrain.Wedesignaview-basedself-adaptiveinterpolationalgorithmandanoffsetlinegeneratingalgorithmtosolveit.Ourresultsshowhighperformancewithgoodvisualquality.

简介：GesarisagreatheroicepiccreatedcollectivelybyTibetans.Forthousandsofyears,ithasbeencirculatingfarandwidewithinTibetanareasandhasbeenlovedbyTibetans.GesarrepresentsanhistoricalpinnacleofancientTibetanculture.AsnotonlyacrystallizationofTibetan'sintelligence,butalsoaculturaltreasurehouse,Gesarisofhighacademicandaestheticvalue.

简介：AbstractThe development of applications for the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) system has increased greatly in recent years, especially in the area of gene therapy by efficient in vivo genome editing. Although great success has been achieved in repairing and rewriting genomes through homology-directed repair coupled with Cas9 nuclease cleavage, its in vivo efficiency is insufficient for gene therapy. Base editing is a next-generation genome-editing tool that does not involve double-stranded DNA breaks and uses components of the CRISPR system together with other enzymes to make point mutations directly in cellular DNA or RNA. Base editors, composed of an engineered deaminase and a catalytically impaired CRISPR/Cas9 variant, are powerful tools for targeted base editing in cells and organisms. In non-dividing cells, base editors can directly transform one base or base pair into another, efficiently installing a point mutation. Undesired by-products of editing are seldom generated during this procedure. Herein we review the different base-editing platforms, including their deaminase recruitment strategies and editing outcomes, and the in vivo delivery of base editors. Additionally, we summarize therapeutic applications of base editing in disorders of the inner ear.

简介：Thewaxygene(Wx)inrice,whichencodesthegranuleboundstarchsynthaseenzyme,isresponsibleforamylosesynthesis.Glutinous(sticky)ricehaslittleornoamylosethatcanbeusedinvariousapplications,suchasbrewing.Inthisstudy,knockoutoftheWxgenewithCRISPR/Cas9technologywasconductedintwoelitejaponicaricelines,Huaidao5(HD5)andSuken118(SK118),aimingtodevelopelitestickyricevarieties.WeachievedsixhomozygousT0plantswithmorethan200bpdeletionintheWxgene,aswellas36wx-HD5and18wx-SK118homozygoustransgene-freeplantsintheT1generation.Theseedsofallthemutantswerewhiteandopaque,similartothoseofstickyrice,andcontainedonly2.6%–3.2%amylose.Resultsofscanningelectronmicroscopyshowedthatthequalityofricedidnotchange.Inconclusion,wesuccessfullydevelopedtwoelitestickyricevarieties.

简介：Precisebaseeditingishighlydesiredinplantfunctionalgenomicresearchandcropmolecularbreeding.Inthisstudy,weconstructedarice-codonoptimizedadeninebaseeditor(ABE)-nCas9toolthatinducedtargetedA·TtoG·Cpointmutationofakeysinglenucleotidepolymorphismsiteinanimportantagriculturalgene.Combinedwiththemodifiedsingle-guideRNAvariant,ourplantABEtoolcanefficientlyachieveadeninebaseeditinginthericegenome.

简介：这篇论文为基于的自我类似论述一个便宜方法由使用高度和反照率地图真实世界的3D表面质地编辑。编辑的基于的2Dtexture接近仅仅做变化到象素颜色或紧张的不同自我类似珍视，这种技术也允许表面几何学和捕获的3D表面质地的反射到用原版不同于那些的照明条件和视场角是ited和relit。在一个给定的地点的单个编辑操作影响所有类似的区域并且在在不同条件下面显示的样品的所有图象上生产变化。后来，表面高度和反照率地图能被用来描述海床地形学和地质的特征，它在许多海洋的进程起重要作用，建议方法能有效地关于海洋的现象的可视化和模拟在应用程序被采用。