简介:Thecalcareousfluvo-aquicsoilwascollectedandamicrocosmstudywascarriedoutwithroot-matandfrozenslicingmethodinlaboratory.ThepHintheroot-soilinterfacewiththecontroltreatmentwasjustslightlylowerthaninthebulksoil.However,theadditionofNH4--NsignificantlydecreasedthepHvalueintheroot-soilinterfaceandtheadditionofNo3-NslightlyincreasedthepHvalueintheroot-soilinterface.ThemagnitudeofpHchangesintheroot-soilinterfacedependedupontheconcentrationsofthenitrogensourcesadded.ThecontentsofCa2-P,Fe-PandAl-Pintheroot-soilinterfaceweremuchloweraftertreatedwithNH4-NandslightlyhigheraftertreatedwithNo3-Ncomparedwithcontroltreatment.Aftertreatedwith100,200and400mg·kg-1NH4+-N,thedeficiencyratesofCa2-Pinthearea0-1mmfromtherootplanewere37.1%,45.9%and57.7%,respectively,thedeficiencyratesofFe-Pwere23.4%,29.1%and38.2%,respectively,andthedeficiencyratesofAl-Pwere25.1%,28.0%and33.2%,respectively.ComparedwiththecontrolthedeficiencyratesofCa8-PinNH4+-NandNo3-Ntreatmentsdecreasedandincreased,respectively,butthedifferenceswerenotobvious.ThecontentsofCa10-PandO-Pintheroot-soilinterfacedidnotsignificantlychangeaftertreatedwithNH4+-NorNo3-N,suggestingthatCa10-PandO-PwereremarkablydifficulttobemobilizedevenatthepresenceofhighconcentrationofNH4+-N.TheloweredpHintheroot-soilinterfaceinducedbytheadditionoftheNH4-Npromotedthetransformationofphosphatesintheroot-soilinterface,enhancedthemobilizationandbioavailabilityofphosphates,andtherebyremarkablyincreasedtheabsorptionofphosphorusbyroots.
简介:热带降雨测量的每日、每周的海表面温度数据扫描观察系统传感器的辐射计地球的使命(TRMM)微波成像器和先进微波被用作在mesoscale内在的海表面强迫模仿越过华南海搬到2003的台风Dujuan的数字模型。数字结果在大气的风地里处于差别在台风中心结果附近显示出那不同SST,显示模型有对SST的快、明显的回答。不同SST在某种程度上影响Dujuan的紧张和轨道并且在眼睛附近在它的降水和潜伏的热流动上有重要影响。对Dujuan的SST影响被改变在海洋表面和空气之间的潜伏的热流动在上面主要完成。
简介:Objective:Toidentifydifferentiallyexpressedlongnon-codingRNAs(lncRNAs)involvedinthemetastasisofepithelialovariancancer.Methods:AninvitroinvasionassaywasperformedtovalidatetheinvasivecapabilityofSKOV3andSKOV3.ip1celllines.TotalRNAwasthenextracted,andmicroarrayanalysiswasperformed.Moreover,ninelncRNAswereselectedforvalidationusingRT-qPCR.Results:ComparedwiththeSKOV3cells,theSKOV3.ip1cellssignificantlyimprovedintheinvitroinvasiveactivity.Ofthe4,956lncRNAsdetectedinthemicroarray,583and578lncRNAswereupregulatedanddownregulated,respectively,inSKOV3.ip1cells,comparedwiththeparentalSKOV3cells.SevenoftheanalyzedlncRNAs(MALAT1,H19,UCA1,CCAT1,LOC645249,LOC100128881,andLOC100292680)confirmedthederegulationfoundbymicroarrayanalysis.Conclusion:LncRNAsclustersweredifferentiallyexpressedinovariancancercellswithvaryingmetastaticpotentials.ThisresultindicatesthatsomelncRNAsmightexertapartialorkeyroleinepithelialovariancancermetastasis.FurtherstudiesshouldbeconductedtodeterminetherolesoftheselncRNAsinovariancancermetastasis.
简介:在声学的logging-while-drilling(ALWD)有限差别及时领域(FDTD)模拟,大练习领子占据,大多数充满液体的地上凿穿并且把地上凿穿液体划分成二薄液体列(半径27公里)。好格子和大计算模型被要求为在工具和形成之间的薄液体区域建模。作为结果,小时间步和更多重复被需要,它增加累积数字错误。而且,在地上凿穿在练习领子和液体之间由于高阻抗形成对照(差别是>30次),完美地匹配的层(PML)的稳定性和效率策划是批评的精确地模仿复杂波浪模式。在这份报纸,我们在一个蹒跚的格子比较了四不同PML实现有限差别及时在ALWD模拟,包括的切开地的PML(SPML),multiaxialPML(MPML),非切开的PML(NPML),和建筑群的领域(FDTD)转移频率的PML(CFS-PML)。比较显示NPML和CFS-PML能比SPML和M-PML更高效地从计算边界吸收指导波浪思考。在大模拟时间,SPML,M-PML,和NPML是数字地不稳定的。然而,M-PML的稳定性能进一步被改进到某程度。基于分析,我们建议CFS-PML方法在FDTD被使用消除数字不稳定性并且为LWD建模在PML层改进吸收的效率。在LWD模拟的CFS-PML参数的最佳的价值基于几千3D模拟被调查。为典型LWD案例,二次的抑制侧面的最好的最大的价值用一d被获得0。为线性转移频率的因素(0)和可伸缩的因素(0)的最大的值的最佳的参数空间取决于PML层的厚度。为典型形成,如果PML厚度是10个格子点,全球错误能被归结为<当PML厚度增加,1%将用最佳的PML参数,和错误减少。
简介:RicecropgrowthandyieldinthenorthIranareaffectedbycropdurationandphenology.ThepurposeofthisstudywastocalibrateandvalidatetheORYZA2000modelunderpotentialproductionbasedonexperimentaldataforsimulatingandquantifyingthephenologicaldevelopment,cropdurationandyieldpredictionofricecropinfluencedbydifferentseedlingages.InordertocalibrateandvalidatethecropparametersofORYZA2000model,atwo-yearfieldexperimentwasconductedunderpotentialgrowthconditio...
简介:开发,生存,蛹的重量,产卵离子,和生命搁置东方烟草芽虫的参数,HelicoverpaassultaGuenée,在人工的节食,辣椒(辣椒frutescensL.),和烟草(NicotianatobacumL.)在实验室被评估。我们发现不成熟的阶段的平均发展时间在烟草(36.2d)上是最长的,在人工的节食(33.5d)在辣椒(34.4d)上中间、最短。到蛹的从鸡蛋的不成熟的幸存在烟草上从31%变化了,43%在辣椒上,并且74%在人工的节食。蛹的重量在人工的节食在烟草,辣椒上的233.1mg/pupa和253.4mg/pupa上从197.4mg/pupa。因为在人工的节食,辣椒,或烟草的幼虫分别地是614,421和334鸡蛋/女性,成年人生的鸡蛋的平均数字高耸了。在女性们在人工的节食,辣椒,或烟草作为幼虫扶养了的成年人的卵巢仍然是鸡蛋的数字分别地是16,26,和42鸡蛋/女性。从在三节食饲养的幼虫开发的成年女性的长寿不是显著地不同的,而从在人工的节食饲养的幼虫的男成年人的长寿更长(16.8d)在烟草(13.8d)和辣椒(13.3d)上为男性比那高耸了。内在,有限,粗野,并且增加的网率为在人工的节食作为幼虫扶养的女性是最高的,为从在辣椒上饲养的幼虫出现的女性为从在烟草上饲养的幼虫出现的女性最低、中间。产生时间和H的双倍工资。assulta最短因为幼虫喂了人工的饮食,从幼虫的中介在辣椒上高耸了,并且最长在烟草上从幼虫高耸了。我们断定人工的食谱是H的最合适的幼虫的食谱。assulta由辣椒,和烟草列在后面。
简介:BACKGROUND:ThedetectionofdifferentialgeneexpressioninbrainispossiblebycDNAmicroarraytechnology,andthescreeningofdifferentiallyexpressedgenesmightprovideabiologicalbasisforgene-targetedtherapyfortumors.OBJECTIVE:TodetectthedifferentialexpressionofgenesamongastrocytomaSHG-44(WHOgradeIV),CHG-5(WHOgradeII),andATRA-treatedSHG-44celllinesbycDNAmicroarray.DESIGN:Laboratoryexperimentsinvitro.SETTING:DepartmentofNeurobiology,theThirdMilitaryMedicalUniversity.MATERIALS:TheexperimentwasperformedattheDepartmentofNeurobiologyintheThirdMilitaryMedicalUniversityoftheChinesePLAfromJanuarytoOctober2007.TheSHG-44cellline(WHOgradeⅣ)wasestablishedbyProf.ZiweiDu,andtheCHG-5cellline(WHOgradeII)wassetupbyProf.XiuwuBianfromtheThirdMilitaryMedicalUniversityoftheChinesePLA.ThecDNAmicroarraycontaining9182knowngeneswaspreparedandprovidedbyDr.YangZhongattheCityUniversityofHongKong.METHODS:ToscreendifferentiallyexpressedgenesfromthegeneexpressionprofilesdetectedbycDNAmicroarraycomparisonsweremadebetweenCHG-5andSHG-44cellsandbetweenSHG-44cellswithorwithouttreatmentwith10μmol/LATRA.SomedifferentiallyexpressedgeneswereselectedrandomlyforNorthernBlotanalysistoconfirmtheresultsofthemicroarray.Thedeterminationcriteriafordifferentialgeneexpressionwereasfollows.①TheratioofCy5signaltoCy3wasgreaterthan2.0orlessthan0.5.②Theresultsofthetriplicatemicroarrayhybridizationsshowedthesametrendinthreeexperiments.③Ageneappearedatleasttwotimesonthetriplicatemicroarrayhybridizations,andthe3rdvaluedidnotshowacontradictorytrend.AnormalizedratioofCy5intensitytoCy3greaterthan2.0orlessthan0.5wasconsideredtorepresentup-regulatedordown-regulatedgeneexpression,respectively.MAINOUTCOMEMEASURES:Theidentificationofgenesthatweresimilarlyregulated(overlapping)dur
简介:到改善AZ91D镁合金的bioactivity和腐蚀抵抗,有新奇microstructured形态学的hydroxyapatite(幸运)涂层经由一个灵巧的热水的方法在AZ91D底层上成功地被准备。不同chelating代理人包括polyaspartic酸(PASP)和ethylenediaminetetraacetic酸(EDTA)被介绍在形态学和腐蚀上调查他们的效果涂的镁的抵抗合金。结果表明准备与的涂层PASP是许多一致像顽童的microspheres镇静,当与EDTA准备的涂层由许多象花的粒子组成了时。而且,与EDTA准备的涂层的crystallinity比的高是大部分与PASP准备的涂层。电气化学的测试表明底层的腐蚀抵抗显著地在与每涂层是涂的以后被改进。在模仿的身体液体(SBF)的涂的样品的沉浸测试证明涂层能逐渐地是biodegraded并且导致钙磷酸盐粒子的形成。
简介:AIMToinvestigatetheexpressionchangeofα-synucleininthebrainofdifferentageAD-likean-imalmodelandtoexplorethepathologymechanismofα-synucleininneuraldegenerationandprotectiveeffectof2kindsofChinesecomponents.METHODSAgenerallyacceptedanimalmodelofAlzheimer'sdisease-APPV717Itransgenic(Tg)mousewasobservedfrom4to16monthold.TheTgmicewererandomlydividedintomodelgroup(4month,10monthand16monthold),Shen-wucapsulewiththedoseof0.4gand1.2g/kg/d,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside(TSG),oneofeffectivecomponentsinPolygonummuhiflorum,atdoseof0.05,0.1and0.2g/kg/d.