简介:Inthispaper,awavelengthdivisionmultiplexing(WDM)transmissionsystemderivedfromthecoherentopticalorthogonalfrequencydivisionmultiplexing(CO-OFDM)withpolarizationdivisionmultiplexing(PDM)and16-orderquadratureamplitudemodulation(QAM)isstudied.Asimulationof80-channelWDMtransmissionsystemwithdatarateof200Gbit/sisbuilt,andthetransmissionperformanceofthesystemisanalyzed.ThesimulationresultsshowthatthesystemQvalueoftheWDMchannelsat16Tbit/swithaspectralefficiencyof7.14bit/s/Hzispotentiallyover10.0dBforalonghaultransmissionupto1800kminastandardsingle-modefiber.
简介:摘要目的探讨腹腔镜改良Bacon,s手术治疗低位直肠癌围术期护理方法。方法术前1日口服20%甘露醇250ml做肠道清洁。术前做好皮肤准备,尤其是脐部用棉签醮肥皂水清洗干净。术后进行肛周护理,定期扩肛、便后坐浴,保持各种引流管通畅,观察并记录引流量及引流液性质。结果42例手术均获成功,无中转开腹及副创伤。手术时间220~315min,术中出血20~280ml,术中肠管断缘病理切片无癌残留,本组病例保肛率100%,术后胃肠功能恢复时间3d,术后并发尿潴留1例,保留导尿管5d后恢复自行排尿。无其他并发症发生,术后住院7~9d。本组获随访,随访时间1~5a,局部无复发,腹壁Trocar的锥孔无肿瘤种植。术后3~6个月自主排便功能恢复。结论加强低位直肠癌腹腔镜改良Bacon,s手术围术期护理,能有效降低低位直肠癌术后并发症,促进排便功能恢复。
简介:基金项目国家自然科学青年基金(项目编号81102482)摘要目的构建人钙联蛋白S100A8的原核表达载体并进行蛋白表达及纯化,以便进一步研究。方法从人单核巨噬细胞THP-1中提取总RNA,逆转录后进行PCR,得到目的基因,与TA克隆载体pMD-T进行连接,得到pMD-S100A8质粒,经BamHⅠ和XhoI双酶切产物或者PCR产物双酶切后,与大肠杆菌pGEX-6P-1表达载体进行连接,得到pGEX-S100A8重组质粒。重组质粒转入E.coliBL21(DE3)表达菌进行蛋白表达。融合GST蛋白用GST柱纯化,冷冻干燥,进行下一步研究。结果成功构建了TA克隆质粒pMD-S100A8及阳性pGEX-S100A8重组质粒,成功诱导蛋白的原核表达。结论纯化得到的蛋白为进一步研究S100A8钙联蛋白的结构和功能提供了参考。
简介:江苏高邮、宝应Ms4.9级地震在极震区造成了数10间农村民居不同程度和不同方式的破坏,出现人员伤亡。震后对地震灾害现场进行震害调查与破坏原因研究,典型的震害特征包括砖砌体、砖木和砖混结构房屋局部倾倒、墙体贯通开裂、房顶瓦片掀翻和烟囱倒塌等。农村民居遭到破坏的主要因素包括:选址不当、施工质量较差、砂浆强度不够、缺少圈梁和构造柱等。同时,分析了竖向地震作用和鞭梢效应等地震效应,讨论了砖砌体和砖混结构抗震设计的加固方法和改善抗震性能的构造措施等,为农村民居的抗震设防和减轻震害损失等提供参考。
简介:Theacquisitionofsecondarychromosomalaberrationsinchronicmyeloidleukemia(CML)patientswithPhiladelphiachromosome-positive(Ph+)karyotypesignifiesclonalevolutionassociatedwiththeprogressionofthediseasetoitsacceleratedorblasticphase.Therefore,theseaberrationshaveclinicalandbiologicalsignificance.T(3;12)(q26;p13),whichisarecurrentchromosomalaberrationobservedinmyeloidmalignancies,istypicallyassociatedwithdysplasiaofmegakaryocytes,multilineageinvolvement,shortdurationofanyblasticphase,andextremelypoorprognosis.Wehaveidentifiedarecurrentreciprocaltranslocationbetweenchromosomes3and12withdifferentbreakpointatbands3q21inthemalignantcellsfroma28-year-oldman.ThepatientwasinitiallydiagnosedashavingPh+CMLinthechronicphase.Thet(3;12)(q21;p13)translocationoccurred4yearsafterthepatientwasfirstdiagnosedwithCMLwhileundergoingtyrosinekinaseinhibitortherapy.Weconfirmedthet(3;12)(q21;p13)translocationviafluorescenceinsituhybridizationassaybyusingwhole-chromosomepaintprobesforchromosomes3and12.Ourfindingsdemonstratethat,similartootherrecurrenttranslocationsinvolving3q26suchast(3;3)andt(3;21),thet(3;12)(q21;p13)translocationisimplicatednotonlyinmyelodysplasticsyndromeandacutemyeloidleukemiabutalsointheprogressionofCML.Thesefindingsextendthediseasespectrumofthiscytogeneticaberration.