学科分类
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2 个结果
  • 简介:Adoublemutantwithstreakedleafandabnormalfloretwasfoundandtemporarilynamedstreakedleafandfloralorgannumbermutant(st-fon).Forthismutant,besideswhitestreakappearedonculm,leavesandpanicles,thenumberoffloralorgansincreasedandfloretscracked.Theextremephenotypewasthatseveralsmallfloretsgrewfromonefloretorbranchrachisinsmallfloretsextendedanddevelopedintopanicles.Byusingtransmissionelectronmicroscopetoobservetheultrastructureofwhitehistocytesofleavesattheseedlingstage,thewhitetissueswhichshowedabnormalplastids,lamellasandthylakoidscouldnotdevelopintonormalchloroplast,andthedevelopmentofchloroplastwasblockedattheearlygrowthstageofplastid.Scanningelectronmicroscopeandparaffinsectionwerealsousedtoobservethedevelopmentoffloralorgans,andtheresultsindicatedthatthedevelopmentoffloralmeristemwasoutoforderandunlimited,whereasinthetwistyleaves,vascularbundlesheathcellsgrewexcessively,orsomebubblycellsincreased.Geneticanalysescarriedoutbymeansofcrossandbackcrosswithfournormal-leaf-colormaterialsrevealedthatthemutantisofcytoplasminheritance.

  • 标签: 遗传分析 突变体 小花 异常 条纹 电子显微镜观察
  • 简介:Apopulationofricebrownplanthopper(BPH)NilaparvatalugenscollectedfromapaddyfieldinHangzhouwassuccessivelyrearedonsusceptiblericeTaichungNative1(TN1)inalaboratoryfreefrominsecticidesformorethan14generations.Thechangesinsusceptibilitytoinsecticidesandecologicalfitnessondifferentresistantricevarietiesweremonitoredineachgeneration.Theresistanceratiotoimidaclopridsharplydeclinedwiththesuccessionofrearinggenerationswithoutinsecticidesfrom359.94-foldatF1to6.50-foldatF14comparedwiththesusceptiblestrain,andtheresistanceratiotochlorpyrifoswasfrom9.90-foldatF1to5.94-foldatF14.Nymphaldurationandweightsofnewlyhatchedfemaleadultsweresignificantlyaffectedbyricevariety,generationandtheirinteractions,butnymphalsurvivalwassignificantlyaffectedbythegenerationonly.Theratioofbrachypterousadultsinmaleswasaffectedbythegenerationandgeneration×varietyinteraction,whereasnodifferencewasfoundinfemales.Nymphaldurationextendedwithincreasinggenerations,andthefemalenymphaldurationwasshorterinthesusceptiblevarietyTN1thanthoseintheresistantvarietiesIR26andIR36.Inaddition,thefemaleadultweightinTN1washigherthanthoseinIR26andIR36.TheseresultsindicatedthattheresistanceoffieldBPHpopulationtoinsecticideswasreversedafterseveralgenerationsofno-exposuretoinsecticides,andtheecologicalfitnessinTN1washigherthanthoseinIR26andIR36.ThesefindingssuggestedtherationalandreduceduseofinsecticidesincombinationwiththemanipulationofresistantricevarietieswouldbeeffectiveforBPHmanagement.

  • 标签: NILAPARVATA LUGENS insecticide-free ECOLOGICAL FITNESS insecticidal