简介:摘要原发性肝癌是目前世界上致死率最高的恶性肿瘤之一,在全世界范围内的发病率有逐年上升的趋势。早期诊断对肝癌患者的预后至关重要。对于肝癌的高危人群,现在最常用的监测手段是定期检测血浆中甲胎蛋白(AFP)水平和影像学检查如超声等。然而,甲胎蛋白的敏感性和特异性都不令人满意。近年来,蛋白组学技术的发展使得筛选新的肿瘤标志物成为可能,各种有希望的新的肿瘤标志物被相继发现,其中高尔基体蛋白73(GP73)最有可能成为更好的诊断肝癌,尤其是早期肝癌的血清标志物。在仅有的少数报道中,其敏感性可达69%,特异性可达90%,而其异构体敏感性和特异性可达90%和100%。除此之外,新发现的肝癌患者血清中高表达的标志物有甲胎蛋白异质体(AFP2L3)、异常凝血酶原(DCP)、α2L2岩藻糖苷酶(AFU)、磷脂酰基醇蛋白聚糖23(GPC23)、肝细胞生长因子(HGF)、转化生长因子β1(TGFβ1)、血管内皮生长因子(VEGF)、粘液素1(MUC21,KL21)等。这些新的血清标志物,正在被各种临床研究进行检测,有希望改变肝癌早期诊断和治疗的现状。我们正在进行GP73的大样本临床研究,取得初步结果。
简介:Tapwaterisoneofthemostcommonlyusedwaterresourcesinourdailylife.However,theincreasingwatercontaminationandthehealthriskcausedbypathogenicbacteria,suchasStaphylococcusaureusandEscherichiacolihaveattractedmoreattention.Themutualismofdifferentpathogenicbacteriamaydiminishantibacterialeffectofantibacterialagents.Itwasfoundthatmaterialsusedformakingpipeandtapplayedoneofthemostimportantrolesinpromotingbacterialgrowth.Thispaperistoreporttheperformanceofaninnovativetype304Cu-bearingstainlesssteel(304CuSS)againstmicrobesintapwater.Theinvestigationmethodologiesinvolvedweremeansofheterotrophicplatecount,contactanglemeasurements,scanningelectronmicroscopyforobservingthecellandsubtractsurfacemorphology,atomicabsorptionspectrometryforcopperionsreleasestudy,andconfocallaserscanningmicroscopyusedforexamininglive/deadbacteriaonnormal304stainlesssteeland304CuSS.ItwasfoundthatthesurfacefreeenergyvariedafterbeingimmersedintapwaterwithpolarcomponentandCuionsrelease.Theresultsshowed304CuSScouldeffectivelykillmostoftheplanktonicbacteria(max95.9%antibacterialrate),andconsequentlyinhibitbacterialbiofilmsformationonthesurface,contributingtothereductionofpathogenicrisktothesurroundingenvironments.