简介：Objective:　Tocomparethedynamicchangesofinterleukin-1(IL-1),interleukin-6(IL-6),andtumornecrosisfactor(TNF)inintermingledskingraftwiththoseinothertypesofskingraftsinrats.　　Methods:　A10%-15%third-degreeburnwascreatedin180Spregue-Dawley(SD)rats.Afterremovingthescar,skingraftswereperformedontheopenwoundsimmediatelywithautoskin(aus,n=54),alloskin(als,n=54)andintermingledskin(n=36).Thatistosay,intheintermingledskingraft,abigpieceofalloskin(mals)wasgraftedfirst,and3dayslater,smallpiecesofautoskin(maus)wereembeddedinthealloskin.Therest36ratsweretakenasthecontrols.AndthebiologicalactivitiesofIL-1,IL-6andTNFingraftsheetsineachgroupweredetectedafterskingraft.　　Results:　ThelevelsofIL-1,IL-6andTNFintheausgroupdecreasedsteadilyaftertheirinitialelevations,whereasinthealsgrouptheyincreasedsignificantlyandkeptonthepeaklevelinthelaterphases.Intheintermingledgroup,thereappearedalowestIL-1levelinthemalsandahighestoneinthemaussimultaneouslyat7(4)days(Thenumberoutofparenthesisisthedaysaftertransplantingwithalloskinsheets,andthenumberinparenthesisisthedaysafterembeddingautoskinsheetsintheintermingledskingraft.Similarlyhereinafter.)afterskingraft(P<0.01),andthehighlevelinthemausabruptlydecreasedat14(11)daysafterskingraft.Atexactlythesamephaseonday7(4),aprominentpeakedIL-6inthemalsoccurred.Inthelaterphases,thelevelsofTNFremainedrelativelylowbothinthemalsandinthemaus.Fromday7(4)on,eachcytokinefluctuationinthemalssynchronizedwiththatinthemaus.Thelongertheposttransplantationperiodlasted,themorethepositivecytokinecorrelatedbetweenthemalsandthemaus.　　Conclusions:　ThelowlevelsofIL-1andTNFmaybeimportantfactorstolightentheintensityoflocalrejectionintheintermingledskingraft.Thetempo

简介：Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.

简介：TheinvivoeffectsofPhytolaccaacinosapoly-saccharidesI(PEP-I)onimmunologiccytotoxicityofmouseperitonealmacrophagesanditsproductionoftumornecrosisfactor(TNF)andinterleukin1(IL-1)werestudied.PEP-I80or160mgkgwasgiveniptwiceevery4day.BothdoseswerefoundtohavesignificantenhancingactivityonmacrophagescytotoxicityagainstS180sarcomacellsandmalignanttransformedfibroblastL929cells.PeritonealactivatedmacrophageswereincubatedwithLPSfor2and24hrstoinduceTNFandIL-1,respectively.TheTNFandIL-1activitiesweretestedfromcytotoxicityagainstL929cellsinanabsorbenceassayofenzymaticreactionandproliferationofthymocytesco-stimulatedassayseparately.TheoptimaltimeforTNFproductionwasfoundonday8.SignificantincreasesinTNFandIL-1wereobserved.IncomparisonoftheeffectofPEP-IonTNFwiththatofknownprimingagentBCG,therewasnodifferencebetweenthem,butPEP-IhadahigheffectonIL-1.Theseresultssug

简介：Objective:Toinvestigatetherolesofinterleukin-2(IL-2)andinterleukin-10(IL-10)inpathogenesisofearlysyphilis.Methods:TheserumlevelsofIL-2andIL-10in48patientswithearlysyphilisweredetectedbyABC-ELISA.Results:(1)ThelevelofIL-2inthepatientswithearlysyphiliswassignificantlyhigherthanthatinhealthycontrols,whilethatofIL-10waslower(P<0.001andP<0.001).(2)ThelevelsofIL-2andIL-10werealmostidenticalinpatientswithprimaryandsecondarysyphilis(P>0.05),aswellasbetweendif-ferentRPRtiters(P>0.05).(3)Aftertherapy,thelevelofIL-2decreasedmarkedly(P<0.05),whilethatofIL-10increase(p>0.05).(4)AsignificantcorrelationwasfoundbetweentheserumlevelsofIL-2andIL-10(r=0.5385P<0.05).Conclusions:Th1up-regulationoccursinpatientswithearlysyphilis,andplaysanactiveroleinfightingagainstTPinfection.

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简介：ToexploretheeffectofrhlL-15onCB-CD34^+stemcellscommittingtoNKcells,CD34^+stemcellswereobtainedfromcordblood(CB)bymagnetic-assistedcellsorting(MACS)method.CD3,CD16andCD56moleculesexpressedoncellsurfaceweredetectedbyflowcytometer.MTFmethodwasusedtotestthecytotoxicityofNKcells.Theresultswerethatstemcellfactor(SCF)alonehasnoeffectonCD34^+stemcells.IL-15stimulatedCD34^+stemcellscommittoNKcells,andSCFshowedstrongsynergisticeffectwithIL-15.ItwasconcludedthatIL-15andSCFplayeddifferentrolesduringNKcelldevelopment,llr15promotedCD34^+stemcellsdifferentiatetoNKcellprecursorandSCFimprovedtheeffectsofIL-15onNKcelldifferentiation.

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简介：GenemodificationoftumorcellstoexpressavarietyofcytokinessuchasIL-2ortheco-stimulatorymoleculeB7.1ledtoincreasedimmunogenicityandreducedtumorigenicityoftumorshasseveralmodelsandthisprocessinvolvesTcells.WehavepreviouslyreporteddecreasedtumorigenicityofthemurineplasmacytomaJ558L(MHCclassⅠ~+andclassⅡ~-)expressingIL-2orB7.l.Whensystemicimmunitywasanalyzed,

简介：Objective:Toinvestigatethetreatmentofspontaneousmetastaticlungcancerbytumorantigen-pulsed,interleukin-12(IL-12)gene-modifieddendriticcells(DC).Methods:Thespontaneousmetastaticlungcancermodel,preparedbyinjectionofthe3LLLewislungcancercellsintothefootpadsofC57BL/6mice,wastreatedbysubcutaneousvaccinationwithtumorantigenpeptidemut1-pulsed,IL-12gene-modifieddendriticcells(DC-IL-12/mut1)derivedfromthenormalbonemorrow.Aftertreatment,thelungweight,thenumberoflungmetastaticnodesandthesurvivaltimeofthetumor-bearingmicewereobserved,andtheNKandCTLactivityweredeterminedrespectively.Themiceweredividedinto8groupswith12miceineachgroup.Results:Comparedwithmicetreatedwithmut1-pulsed,controlLacZgenemodifiedDCanduntreatedDC,tumor-bearingmicetreatedwithDC-IL-12/mut1hadthelightestlungweights(P<0.01),theleastlungmetastaticnodenumber(P<0.01),thelongestsurvivaltime(P<0.01),alsowiththeinductionofpotentCTLactivity(P<0.01)andNKactivity(P<0.01).Conclusion:Tumorantigen-pulsed,IL-12gene-modifieddendriticcellshavesignificanttherapeuticeffectsonthespontaneousmetastaticlungcancer,providinganewapproachtotreatmentoflungtumors.

简介：Interleukin-12(IL-12)isacriticalcytokinerepresentingthelinkbetweenthecellularandhumoralbranchesofhostimmunedefenseapparatus.IL-12-inducedcytotoxiclymphocyte(CTL)developmentisacentralmechanisminimmuneresponsesagainstintracellularinfectiousagentsaswellasmalignantgrowth.However,themolecularbasisoftumor-specificCTLresponsesmediatedbyIL-12remainspoorlydefined.Inthisstudy,weaddressedthisissueinacomprehensivemannertoprobeintoIL-12-inducedanti-tumorresponsesbyglobalgeneexpressionprofilingofmRNAexpressioninCD8+TcellsinatransplantablesyngeneicmousemammarycarcinomamodeltreatedornotwithrecombinantIL-12.AstrongtumorregressionwasinducedbytheIL-12treatment.AnintrospectionofdifferentialgeneexpressionatanearlystageoftheIL-12-initiatedCTLactivationrevealsinterestinggenesandmolecularpathwaysthatmayaccountforthemarkedtumorregression,andislikelytoprovidearichsourceofpotentialtargetsforfurtherresearchanddevelopmentofeffectivetherapeuticmodalities.Cellular&MolecularImmunology.2004;1(5):357-366.

简介：１＋１＝？●魏鹏程前不久，县国税局召开征管改革现场会。两位老所长看完我们所税负核定清册后，问我们：“你们月初核定的税款，当月能如数收上来吗？”我们回答：“能”。两位老所长不信的摇摇头，并笑着说：“在农村收税，要想１＋１＝２，哪是不可能”。听了老所长的...

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简介：我们网罗的对象。随想随写,随写随想。所有奇闻趣事,所有奇思妙想,都是

简介：在2005年“国家电网公司农村供电所安全管理年”活动中，江苏省沛县供电公司农电管理所大胆创新，自3月份以来，在全县农村供电所推行了一种新的安全管理运行机制——4个1=1，为已经连续实现安全生产15年的沛县供电公司注入了新的活力。

简介：在数学竞赛中往往碰到形如ab±(a+b)+1=(a±1)(b±1)的式子，我们若巧妙地应用它去解决问题，往往能收到事半功倍的效果，现结合例子说明常用技巧。

简介：不要悲哀,不要糊里糊涂地迷失了自我;世界上没有绝对的事,只要努力,一切都会好起来的。

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简介：Theground-stateandthermodynamicpropertiesofquantummixed-spinchainsof1/2-1/2-1-1and3/2-3/2-1-1areinvestigatedbyaquantumMonteCarlosimulationwiththeloop-clusteralgorithm.For1/2-1/2-1-1chain,wefindithastwophasesseparatedbyanenergy-gapvanishingpointintheground-state.For3/2-3/2-1-1chain,thenumericalresultsshowtwoenergy-gapvanishingpointsisolatedbydifferentphasesinitsground-state.Ourcalculationsindicatethatallthesegroundstatephasescanbeunderstoodbymeansofvalence-bond-solidpicture,andthethermodynamicbehavioratfinitetemperaturesiscontinuousasafunctionofparameterα=J2/J1.

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