简介：Objective:Tomakeaclinicalanalysisonthebasisof36casesofatypicalgenitalherpes(GH)patients.Methods:Thirty-sixcasesofatypicalGHwerediagnosedclinically,andtheircasehistories,symptomsandsignswererecordedindetailandfollowedup.Polymerasechainreaction(PCR)wasadoptedfortestingHSV2-DNAwithcotton-tippedswabs.Enzyme-linkedimmunosorbentassay(ELISA)forserumanti-HSV2-IgMwasdonetoestablishadefinfiivediagnosis.Otherdiagnoseswereexcludedatthesametimebytestingforrelatedpathogensincludingfungi,Chlamydia,Mycoplasma,Treponemapallidum,gonococci,Trichomonas,etc.Results:ThemainclinicalmanifestationsofatypicalGHwere:(1)smallgenitalulcers;(2)inflammationofurethralmeatus;(3)nonspecificgenitalerythema;(4)papuloidnodulesontheglands;(5)nonspecificvaginitis.Twenty-threecases(64%)testedbyPCRwereHSV2-DNAsera-positive,and36cases(100%)anti-HSV2-IgMsera-positivebyELISA.Conclusion:atypicalHSVisdifficulttobediagnosed.ButthecombinationofPCRandELIASwillbehelpfultothediagnosisofatypicalHSV.

简介：Objective:Todetectandquantitategenitalherpessimplexvirus(HSV)DNAinspecimensfrom100patientsclinicallydiagnosedwithgenitalherpes.Methods:PolymeraseChainReaction(PCR)andenzyme-linkedimmunosorbentassay(ELISA)wereusedwithastandardcurveofDNAcopiesofHSVasquantitativecontrast.Results:Ninety-threecaseswereconfirmedHSVpositiveand7caseswerefoundtobenegative.Therewere58casesofHSV-2(62.4%)and35casesofHSV-1(37.6%)amongthe93positivecases.ThenumberofDNAplasmidsrangedfrom115to1.1×l0^5per250pLamongthe93positivesamples(mean=7.1×10^4/250μL).ThenumberofHSVDNAplasmidsrangedfrom136to1.1×l0^5copiesper250pL(mean=7.6×10^4)amongthosewithHSV-2,and115to9.4×10^4per250pL(mean=6.3×10^4)amongthosewithHSV-1.Meanwhile10μLofextractedanddissolvedDNArandomlytakenfrom8eachofHSV-2andHSV-1samplesweretested.ThenumberofHSV-2DNAplasmidsrangedfrom35copiesto2.7×10^4(Mean=l.8×10^4)andthenumberofHSV-1DNArangedfrom29to2.5×10^4(Mean=1.6×10^4).Inthe7negativecases,thequantityofHSVplasmidswaszero.Conclusion:ThesensitivityofELISAquantitation(93%)isequaltothatofSouthernblot.ThesensitivityofPCRfordiagnosisis91%,and88%forPCRtyping.

简介：Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.

简介：AbstractIntroduction:Genital herpes is one of the most common sexually transmitted infections worldwide. Most genital herpes occur on the genitalia, perineum, buttocks, upper thighs, or perianal areas. However, there were reports of rare cases of genital herpes on urethral orifice.Herein, we report a case of a patient who was diagnosed with urethral orifice genital herpes, a place likely to be ignored.Case presentation:A 53-year-old woman presented with vagina pruritus at first, with subsequent burning sensation around urethra. Mycostatin and mycospor were administered for the treatment of the pruritus. Then levofloxacin was prescribed. Finally, the patient was given treatment with acyclovir. Different from other cases, the investigation showed herpes simplex virus type 1 DNA positive and herpes simplex virus type 2 DNA negative by PCR. The patient was therefore diagnosed with genital herpes.Discussion:Most genital herpes happen on the genitalia, perineum, buttocks, upper thighs, or perianal areas. Few cases have been reported that genital herpes occurred on urethral orifice till now.Conclusion:If there is no typical symptoms that patient present with herpes simplex virus type 1 or 2 positive, urethral orifice genital herpes should be considered.

简介：Objective:ThisstudyanalyzedtheTlymphocytesandThl/Th2typecytokineprofileshiftintheperipheralbloodofpatientswithrecurrentgenitalherpes(RGH).Methods:Immunofluorescentstainingofcellsurfaceantigenandintracellularcytokines(IL-2,IL-4,IL-12,IFN-r)inperipheralbloodfrom20RGHpatientsand10controlswereanalyzedusingflowcytometrictechniques.Results:RGHpatientshadsignificantlylowerlevelsofCD3^+Tcells,CD4^+TcellsandCD4^+T/CD8^+Tcellsratiocomparedtocontrollevels(P<0.001),andIL-2-producing,IFN-r-producingandIL-12-producingTcellswereincreasedinRGHpatients(CD4^+T:P<0.001,CD8^+T:P<0.05respectively),whereasIL-4-producingTcellswereincreasedinRGHpatientscomparedtocontrols(CD4+T:P<0.05;CDS^+T:P<0.001respectively).Conclusions:RGHpatientshaveTlymphocytesubsetvariationsandThl/Th2cytokinechanges.TheincreaseinTh2cellsThl/Th2imbalancemayhaveimportantimplicationsforRGHpathogenesis.

简介：Objective:TostudytheeffectoftraditionalChinesemedicineantiviralcapsulesinthetreatmentofgenitalherpes.Methods:Usingfemaleguineapiggenitalherpesastheanimalmodel,thisstudyusedoraladministrationoftwoformulationsofantiviralcapsules(AC)andobservedtheeffectonvaginalHSV-2titersandvulvarsymptoms.CellcultureswerealsousedtoexaminethedirectinactivationofHSV-2bytheantiviralcapsulesandthesuppressionofHSV-2viathreedrugadministrationmethods.Results:Therewasnosignificantdifferenceofmeanvaginalvirustitersbetweentheantiviralcapsulegroupsandthatofthepositiveacyclovir(ACV)control(P>0.05).Meanvulvarsymptomscoresofthetwoantiviralcapsulegroupswerealsosignificantlylowerthanthatofthesalinenegativecontrolgroupondays2,3,5,7and8(P<0.05)andsimilartothatoftheACVcontrol(P>0.05).CellcultureshowedtheminimuminhibitoryconcentrationsofantiviralcapsulesNo.1andNo.2were0.390625mg/mland1.5625mg/ml,respectively.Conclusion:ThetraditionalChinesemedicineantiviralcapsuleshadsuppressiveeffectsonHSV-2inbothanimalmodelGHandinvitrocellculture.

简介：TostudytheroleofTh1/Th2cytokinesinthepathogenesisofrecurrentgenitalherpes(RGH),andtobetterunderstandtherelationshipbetweenthem.Methods:Atwo-colorimmunofluorescentstainingofcellsurfaceantigenandintracellularcytokinesforflowcytometricanalysiswasusedforCD3,IL-2,IL-10,IL-12,IFN-yandTNF-ainCD3^+T-lymphocytesinactivatedperipheralbloodmononuclearcellsofpatientswithRGH.Results:Comparedtocontrols,patientswithRGHshowedfewerCD3^+Tcells(P<0.05)andIL-2producingandIFN-7producingTcells(P<0.02andP<0.001,respectively)afterinvitrostimulationwithPMAandionomycininthepresenceofaproteintransportinhibitor.MoreIL-10producingandIL-12producingTcellswerefoundinpatientswithRGH(P<0.01).TherewasnosignificantdifferenceinthenumberofTNY-αproducingcellsbetweenRGHpatientsandcontrols(P<0.05).Conclusion:RGHpatientsshowedrelativelymoreTh2cytokines.TheimbalancebetweenThlandTh2cytokinesresultsininhibitoryeffectsonaseriesofcell-immuneresponses,whichmayplayanimportantroleinthepathogenesisofRGH.

简介：Objective:Tostudytheroleofmonocytesinthepathogenesisofgenitalherpes.Methods:TNF-aandIL-6levelsin27casesofgenitalherpesweredetectedbyenzymelinkedimmunosorbantassay(ELISA).HLAclassⅡantigenexpressiononmonocytesweredetectedbyanalkalinephosphataseanti-alkalinephosphatasemethod.Results:Comparedwithnormalcontrols,levelsofTNF-aandIL-6secretedbymonocytesrespondingtoLPSmitogeninvitroweresignificantlydecreased[(3.13±0.44ng/ml)vs(4.68±0.54ng/ml),P<0.05and(3.32±1.06ng/ml)vs(6.46±1.94ng/ml),P<0.05,respectively].HLAclassⅡantigenexpressiononmonocytesinthegenitalherpesgroupwasalsosignificantlydecreased[HLA-DR(67.48%±1.51%)vs(81.03%±1.32%),P<0.01andHLA-DQ(29.54%±1.15%)vs(37.63%±1.79%),P<0.01respectively].Conclusion:Thesefindingssuggestthatthedecreasedmonocytefunctionmaycontributetothepathogenesisofgenitalherpes.Augmentingorinducingmonocytefunctionmaybeimportantintheprevention,treatment,andreductionofgenitalherpescases.

简介：Objective:ToevaluatetheclinicalapplicationofmultiplexPCRinthedetectionofTreponemapallidum,Herpessimplexvirus(HSV),andHaemophilusducreyi.Method:ThreestandardstrainswereusedtosetupamultiplexPCR(MPCR)fordetectingsyphilis,herpesgenitalis,andchancroidsimultaneously.Samplesfrom122patientswithgenitalulcerdisease(GUD)weresubjectedtoMPCRandtheresultswerecomparedwiththeseofdark-fiddmicroscopyandTPserology,HSVanligenELISA,andH.ducreyiculture,Result:Inthe122patientswithGUD,MPCRidentified34casesofT.palliduminfection,40casesofHSVinfection,and2casesofmixedinfectionofT.pallidumandherpes.NopositiveresultsofH.ducreyiwerefound.ThesensitivityofMPCRtoT.pallidumandherpeswas100%and93.3%,respectivdy.Thesensitivitiesofdark-fieldmicroscopyandTPserology,HSVantigenELISA,andH.ducreyiculturewas35.3%,50%and100%,respectively.Conclusion:MPCRshowedarelativelyhighersensitivityforT.pallidumascomparedwiththeroutinetechniques.AlthoughitssensitivityforHSVwasnotasgoodasthatofantigenELISA,italsoyieldedahighdetectionrate.MPCRcandetectmorethanonepathogen.Itissimple,quick,sensitive,andsuitableforclinicaluseorepidemiologicalinvestigation.

简介：Althoughmanytraditionaltherapiesareavailableforgenitalwarts(GW)，oneofthemajorproblemsinthetreatmentofGWisahighrecurrenencerate．Inordertoresolvetheproblemofrecurrence，newtheraputicapproacheshavebeeninvestigated．Newapproachesbeingexploredinclude5-fluorouracil／epinephrineinjectablecollagengel,5％imiguimodcreamandhumanpapillomavirusvaccine．Fluorouracil／epinephrineinjectablecollagengelachieveshighconcentrationoffluorouracilatthesiteofinjection．Imiguimodisanovelimmune-responesmodifier,givinggoodclearanceratesandtolerablesideeffects．AlthoughtheuseofanHPVvaccineisfaraway，preliminarystudiesshowthatitmaybepossibletodevelopsuitableprophylacticandtherapeuticvaccinestogenitalwarts．

简介：AbstractObjective:Genital chlamydial infection is a globally common sexually transmitted infection caused by Chlamydia trachomatis. This study aimed to describe the epidemiological characteristics of the disease reported in China in 2019.Methods:Genital chlamydial infection cases were identified in 105 national sexually transmitted disease surveillance sites from the National Notifiable Infectious Disease Reporting System, which covers 31 provinces, autonomous regions, and municipalities in Chinese mainland. Data were collected and used to calculate the overall incidence, and the incidences per surveillance site, province, sex, and age group.Results:In 2019, there were 50,874 new cases of genital chlamydial infection reported in China, corresponding to an incidence of 55.32 cases per 100,000 population, and comprising a 9.98% increase from 2018 (50.30 cases per 100,000 population). The highest incidences of genital chlamydial infection occurred in Zhejiang, Guangdong province, Guangxi autonomous region, and Hainan province (average 137.49-233.37 cases per 100,000 population). The incidence of genital chlamydial infection was much higher in females than males, with a female-to-male ratio of 3.09 (84.55 per 100,000 population vs 27.35 per 100,000 population). The highest incidence of genital chlamydial infection was reported in the 20-24-year age group (184.70 cases per 100,000 population), followed by the 25-29-year age group (180.79 cases per 100,000) and 30-34-year age group (151.53 cases per 100,000 population). The highest proportion (68.30%) of genital chlamydial infection was reported from general hospitals, followed by maternal and child health hospitals (25.59%).Conclusion:The incidence rate of genital chlamydial infection in 2019 has an increase trend from 2018 and be a common sexually transmitted infection in China. The incidence is much higher in females than in males. Sexually active young adults are the highest risk population. There is substantial geographic diversity in incidence, with the highest incidence occurring in the southeast coastal areas with a developed economy. Epidemic of genital chlamydial infection in China should be concerned, and its surveillance needs to be improved.

简介：目的将为疱疹带状疱疹观察包围针灸的临床的效果。有疱疹带状疱疹的六十个病人随机被划分成二个组，一个治疗组和控制的方法组织，在各个的30个盒子。在治疗组的盒子与在有斑点的区域上包围针灸，Acyclovir的静脉内的注入和Ne-Ne激光放射被对待。当时，那些与Acyclovir的静脉内的注入和Acyclovir的热门申请在控制组被对待软膏。治疗学的效果在治疗的二堂功课以后在两个组被估计。治疗组显然是的结果更好处于有效的率比控制组织，水泡的地势，水泡枯竭，疼痛的地势和疼痛的完全的地势的时间(P<0.05)。结论包围针灸，与Acyclovir和He-Ne激光放射的静脉内的注入结合了，在为疱疹带状疱疹的治疗学的效果是显著的并且值得临床的申请。

简介：Objective:Toinvestigatetheefficacyofcombina-tionofcircumcisionandmicrowaveongenitalwartsinuncircumcisedmen.Methods:Arandomized,prospectivestudyof109uncircumcisedadultmenwithgenitalwartswascon-ductedinaSTDclinicinZhanjiang,Guangdong.Onegroup(n=54)receivedmicrowavetherapyonly,whiletheothergroup(n=55)wastakenthecombinationofcircumcisionandmicrowavetherapy.Therecurrenceswereobservedattheendofmonths3,6and12,andoperativecomplicationswerealsorecorded.Results:Therewerenosignificantdifferencesinthemeanageanddurationofthediseasebetweentwogroups(P>0.05).Noseriousoperativecomplicationsweredocumented.Therecurrencerateincircumci-sionplusmicrowavegroupwasmarkedlylowerthanthatinmicrowavegroup(12.7%vs29.6%,P<0.05),whilethedifferencesinearlyandlaterecurrencesbetweentwogroupsshowednostatisticalsignificance(P>O.05).Conclusion:Circumcisioncanbesafelyperformedunderlocalanesthesiainanoutpatientsetting.Com-binationofcircumcisionandmicrowavecanproduceexcellenteffectaswellaslesstissuedamage,therefore,itmaybeidealforuncircumcisedpatientswithextensivecondvlomas.

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简介：Objective:ThisstudyexaminedHerpesSimplexVirus(HSV)subclinicalsheddinginthegenitaltractofpatientswithgenitalherpes(GH)ornon-gonoccalurethritis(NGU).Method:SwabswerecollectedafterexposuretorashandgenitaltractduringGHrelapseorremissiononaweeklybasisforfourtosixweeks.NGUpatientswithnegativechlamydiaandmycoplasmatestswerealsoswabbedforasimilarduration.AllswabsunderwentHSVDNAdetectionwithquantitativePCR.Result:TherewasasignificantdifferenceintherateofasymptomaticHSVsheddinginurinarytractscomparingGHandthecontrolgroupandcomparingNGUandthecontrolgroup(P<0.05).TherateofHSVsheddingwas22%,9.8%and3.3%forGH,NGUandcontrolgroupsrespectively.TherateofHSVsheddingwas21.7%(20/92)forpatientswithactiveGHand23%forthoseinremission.TheHSVpositiveratewassignificantlyhigherinthegroupwithpatientswhohadmorethansixrelapseswithinoneyearcomparedtothegroupofpatientswithlessthansixGHrelapses.Conclusion:ThereisHSVsubclinicalsheddingintheirgenitaltractduringactiveGHandremission.SubclinicalHSVsheddingismorecommoninpatientswithmorethansixGHrelapsesperyearcomparedtoGHpatientswithfewerrelapses.Approximately9.9%ofNGUpatientswithnegativechlamydia,mycoplasmatestingwasfoundtohavesubclinicalHSVinfection.

简介：ClinicalDataInthisgroup,totally17caseswereobserved,including10malesand7females,withtheagerangingfrom18to67yearsoldandwiththedurationfrom2daysto1year.

简介：AbstractRecently, death from herpes B virus (Cercopithecine Herpesvirus 1) infection was reported in China, reminding us to be alert to the risk of transmission and infection with herpes B virus. Herpes B virus is a zoonotic pathogen that can cause fatal encephalomyelitis in humans. The virus naturally infects rhesus monkeys, causing diseases like human herpes simplex virus (HSV) infection, which are often asymptomatic or mild in the rhesus macaque. However, herpes B virus infection can be fatal to humans. Without timely treatment, the mortality rate of herpes B virus infection is as high as 70%-80%. To date, approximately 50 cases of human herpes B virus (HBV) infection have been reported worldwide. Most cases are related to direct contact with macaques, such as scratching, biting, or mucosal contact with monkey body fluids or secretions. Although the risk of human-to-human transmission is low, the widespread prevalence of the virus among monkeys, the high mortality of infected persons, and the severe neurological sequelae of survivors render this virus an important zoonotic pathogen that threatens human beings. Biorisk-related training programs for at risk personnel and timely treatment after exposure to the B virus can reduce infection rate and mortality. The early initiation of antiviral therapy prevents severe disease or death after the rapid diagnosis of human B virus disease. Identification of risk factors is essential in controlling the spread of the herpes B virus in the population at risk.

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简介：Theauthortreated16casesofsequelaeofneuralgiaafterherpeszosterwithacupuncture,moxibustionandpointinjectionintheaffectedregion,andallcaseswerecured.