简介：Herpeszosterisanacutedermatosiscausedbychlckenpoxvirusinflammationandmarkedmainlybyviolentpainandcord-likeherpesontheskinsurface.IntraditionalChinesemedicine,itistermedas"SheChuanChuang"(蛇串疮).Theintolerablepainexitsinthewholeprocessofthediseaseevenafewweeksormonthsafterdisappearanceoftheherpes,causingthepatienttosufferalot.FromAprilof1997toJuneof2002,theauthoradoptedacupoint-injectionandacupuncturetherapiestotreat19casesofherpeszosterandachievedsatisfactoryresults,hereisthereport.

简介：无

简介：Toinvestigatetheetiologyofgenitalulcerdisease(GUD)amongpatientsattendingsexuallytransmitteddisease(STD)clinicsinGuangzhou,China.BetweenSeptember8,1998,andAugust9,assessed.ClinicaletiologyofGUDwasdependentonincludingthefollowing:darkfieldmicroscopyandserologyforTreponemapallidum(TP),swabsofgenitalulcerforsimultaneousdetectionofHSV,TP,Haemophiusducreyi(HD),Humanpapillomavirus(HPV),andserologyforHIVinfection.Twohundredthirtymenandthirty-sevenwomenwithamedianageof33.4(range16-74years)wereanalyzed.TheetiologyofGUDwassyphilis(26.59%)(71/267),genitalherpes(17.60%)(47/267),condylomataacuminata(4.87%)(13/267),candidiasis(3.37%)(10/267),andmultipeinfection(6.74%)(18/267).TheseroprevalenceofHIVwas0.07%(2/267).Noetiologywasidentifiedin50.56%(135/267).TheetiologyofGUDamongSTDpatientsinourareawasmultifactorialwithapredominanceofsyphilisandgenitalherpes.BasedonthislimiteddataobtainedatSTDclinics,HIVinfectionwasnotcommon.

简介：在影响男繁殖的道的开发的基因的单个核苷酸多型性(SNP)与男泌尿生殖器的畸形被联系了。然而，没有研究测试了在象阴肛部的距离(AGD)那样的SNP和中间的显型之间的关系，anoscrotal距离(ASD)和阴茎宽度(PW)。我们测试了在影响男生殖器的开发的八基因的24普通SNP是否为未来家庭从学习在106个健康男婴儿与中间的显型被联系。我们从口腔的油迹和线性回归模型使用了DNA为covariates与调整估计在阴肛部的大小和SNP遗传型之间的关系。我们发现rs2077647G等位基因，在雌激素受体alpha(ESR1)的编码区域定位了，与更短的AGD被联系(P=0.02；−；7.3公里，95%；信心间隔(CI)：−；11.6到−；3.1)，并且rs10475T等位基因，在3′定位了；激活抄写因素3的untranslated区域(ATF3)，与更短的ASD被联系(−；4.3 ；公里，95%；CI：−；7.2到−；1.4)，尽管这结果不是重要的(P=0.07)在为多重比较控制以后。我们没观察在测试的PW和SNP之间的协会。为在调整在男生殖器的开发期间发信号的雌激素的基因的二SNP的次要的等位基因与AGD和ASD被联系，尽管协会的意义是边缘的。我们的调查结果suggest ；那AGD和ASD被可继承的factors 影响；在知道与象hypospadias和cryptorchidism那样的坦率的男生殖器的畸形被联系的基因。

简介：Thetherapeuticeffectofherpessimplexvirusthymidinekinase/ganciclovir(HSV-tk/GCV)systemonhepatocellularcarcinomawasstudiedinthisexperiment.Thetk-containingretroviralrecombinantswereusedtoinfecthepatomacells(BEL-7402)andthecellsweretreatedwithganciclovir(0-100μg/ml).TheresultsshowedthatHSV-tkgenecouldbeefficientlytransferredinvitrointohepatomacellsandstablyexpressed.Thegrowthpotentialofthetk-containingcellswassignificantlyinhibitedbyGCV(P<0.01)ascomparedtothenon-tk-containingcells.TheantitumoreffectofHSV-tk/GCVsystemwasalsoproducedexvivointk-containingtumorofnudemiceascharacterizedbyamarkeddecreaseintumorgrowthafterGCVtreatmentcontrarytoaprogressiveenlargementofnon-tk-containingtumors.Althoughthehistologicalexaminationdemonstratedthattheefficiencyofthegenetransferwaslessthan30%,thekillingeffectofHSV-tk/GCVsystemonhepatocellularcarcinomawasstillsignificantlygenerated.ThegropermechanismofHSV-tkgenetherapyonhepatictumorreferredas“bystandereffect”intherapeuticapproachhasnotbeenfoundinthisstudyandrequiredtobeexploredfurther.

简介：无

简介：

简介：

简介：ToobservetheeffectofGardeniaextractZGontheadsorptionquantityofherpessimplexvirustype1(HSV-1)soastoexplorethemechanismofitsantiviralactivity,fluoresceinisothiocyanate(FITC)wasusedasthefluorescentprobetolabelvirusesandheparinsodiumwasusedascontrol.Meanwhile,theeffectofGardeniaextractZGontheadsorptionquantityonthesurfaceofHep-2cellswasdeterminedbyflowcytometry.ItwasdemonstratedthatadsorptionofHSV-1onthesurfaceofHep-2cellsexhibitedthecharacterofsaturationandspecificityandheparinsodiumcouldpreventattachmentofvirusesonthesecells.Theseresultsareinaccordwiththosereportedpreviously.Itwasalsoprovedthatthemannerofdrug-usepriortoadsorptionorsimultaneoususeofdrugandadsorptionwasbetterthanadsorptionpriortodrug-use,andtheinhibitionratesoftheformerandlattermannerwere84.76%and82.92%respectively.Threemannersofdrug-usewithGardeniaextractZGwerealleffectivetoreducetheadsorptionquantityofviruses,especiallythemannerofsimultaneoususeofdrugandadsorptionwithanadsorptioninhibitionrateof68.46%.Fromtheaboveobservation,itisapparentthatthemechanismofanti-viralactivityofGardeniaextractZGmaybeviaseveralstepsinvolvedintheHSV-1adsorption.

简介：Sixty-fivecasesofherpeszosterwererandomlydividedintotheHe-Nelasergroup(33cases)andpolyinosinicacidgroup(32cases).TypeJIHe-NelaseruniversaltreatmentapparatuswasusedtoirradiateinjuredcutaneousregionsandtoradiateHegu(LI4)andZusanli(ST36)pointswithphotoconductivefibersonceeverydayintheHe-Nelasergroup;2mlofpolyinosinicacidwasin-tramuscularlyinjectedonceeveryotherdayintheothergroup.Ofthe65cases63werecured,andthetwocaseswithsequelaofneuralgiawereallinthelattergroup.Painwasdisappearedandscabswereformedrespectivelyafter1.48and5.76daysoftreatmentwithHe-Nelasertherapy,andafter10.5and10.4dayswithpolyinosinicacidtreatment.Thedifferencesoftherapeuticeffectsbetweenthetwogroupswerenoticeablysignificant(P<0.01).

简介：无

简介：TherearesomeIndicationsthttherpesaimplexvirus(HSV)maybemutagenic.SpecificchromosomalchangeshavealsobeendemonstratedInculturedcellsinfectedwithHSV.TofurtherInvestigatethemutagenicactivityofHSVtype2(HSV-2)weusedmouseskinasamodelsystemforcardnogenetls.Inoculationofthebackskinof4-week-oldSencarmkewithlivevirustwiceperweekforoneweekorwithInactivatedvirustwiceperweekfortwoweekswasusedtoInitiatethemouseakin.AfterInitiationwithHSV-2,12-O-tetradecanoylphorbol-13-acetale(TPA)wasappliedtwiceweeklyfor50weeksasapromoter.Duringaperiodof52weeks,noskincarcinomawasfoundIntheexperimentalgroups,whereas55%ofcontrolmicetreatedwith9,10-dlmethy1-1,2-benzanthracene(DMBA)andthenwithTPA-developedskincarcinoma.TheresultsdemonstratethatHSV-2couldnotsubstituteforDMBAinthisanimalmodeloftwo-stageskincarcinogenesis.

简介：Objectives:TodevelopamethodofsimultaneousPCRdetectionofHaemophilusducreyi,Treponemapallidum,andHerpesSimplexVirusTypes1and2fromgenitalulcersamongpatientsattendingSTDclinicsinGuangzhou,China;andevaluatetheclinicalapplicationofmultiplexPCR(M-PCR)assayfordiagnosingtheetiologyofgenitalulcerdiseases(GUD).Methods:244patientswithagenitalulcerwereevaluated.ClinicaletiologyofGUDwasbasedonphysicalappearanceandmicrobiologicevaluationsthatincludeddarkfieldmicroscopyexamination(D-F)andserologytestforsyphilis(STS).SwabsofeachgenitalulcerweretestedforHSVantigenbyenzymeimmunoassay(EIA)andprocessedinanM-PCRassayforsimultaneousdetectionofT.pallidum,HSVandH.ducreyi.Results:ThestandardstrainsofT.pallidum,HSVandH.ducreyiwereamplifiedbyM-PCR,producingamplifiedproductsof260bp,432bp,170bp,respectively.ThesensitivityofM-PCRis102pgDNA.M-PCRassayforT.pallidum,HSVandH.ducreyishowedgoodagreementwhencomparedwithD-FdetectionforT.pallidum,STS,H.ducreyicultureandEIAforHSVantigen(Kappascoresare0.774,0.704,0.793,0.756,respectively).Conclusions:TheM-PCRisaconvenient,accurateandreliableassayforthedetectionofT.pallidum,HSVandH.ducreyifromgenitalulcers,andcanbeusedasamethodofdiagnosingtheetiologyofGUD.

简介：AIM:TodeterminetheassociationbetweenchlamydialconjunctivitisandgenitalinfectionbyChlamydiatrachomatis,MycoplasmagenitaliumandCandidaalbicans,inadditiontothepossiblerelationshipbetweenculturedbacterialpathogensandoculogenitalchlamydialinfection.METHODS:Thisstudywasperformedon100(50symptomaticand50asymptomatic)womenattendingtheGynecologicalandObstetricoutpatientclinicofAlzahrahospital,AlazharUniversity.Simultaneouslyaconjunctivalswabwastakenfromthesepatients.Polymerasechainreaction(PCR)wasdoneonDNAextractedfrombothvaginalandconjunctivalswabsamples.Cultureforbothvaginalandconjunctivalswabswasalsodone.RESULTS:Candidaalbicanswasthepredominantorganismisolatedbyculturein20%and40%ofconjunctivalandvaginalswabsrespectively.BythePCRmethod,ocularChlamydiatrachomatiswaspresentin60%ofsymptomaticwomen,whilegenitalChlamydiatrachomatisinfectionwaspresentin30%ofsymptomaticwomen.Theresultsofthismethodalsoindicatedthat25/50(50%)vaginalswabswerepositivewithPCRforCandidaalbicansversus15/50(30%)werePCRpositiveinconjunctivalswab.Mycoplasmagenitaliumwaspresentinonly10%ofvaginalswabs.ConcomitantoculogenitalPCRpositiveresultsforChlamydiatrachomatisandCandidaalbicanswere30%and28%respectively.CONCLUSION:OcularChlamydiatrachomatiswasassociatedwithgenitalChlamydiatrachomatisinahighpercentageofwomenfollowedbyCandidaalbicans.CulturedbacterialorganismsdonotplayaroleinenhancementofChlamydiatrachomatisinfection.

简介：Toestablishasensitiveandspecificmethodforseroepidermiologicaldetectionofhumanher-pesvirus8(HHV-8)infection,threepotentantigenicproteinsencodedbyopenreadingframes(ORFs)K8.1,65and73CingenomeofHHV-8wereproducedasglutathioneS-transferasefusionproteinintheprokaryoticexpressionsystemandwasusedasantigenfortesting.Therecombinantfusionproteinex-pressedintheprokaryoticexpressionvectorE.coliBL21waspurifiedbyglutathioneSepharose4Baffin-itychromatographyandwasquantitatedwithSDS-PAGE.Allthese3fusionproteinsproducedinthepro-karyoticexpressionsystemshowedgoodimmunogenicityasdemonstratedbyWesternblottingandcouldberecognizedbymixedseraofpatientswithKaposi′ssarcoma(KS).Theimmuno-reactivitiesofthesingleorcompoundfusionproteinweredeterminedbymeansofELISAandcomparedwiththetraditionalimmu-nofluorescenceassay(IFA)todeterminetheirsensitivityandspecificityofthetest.Itwasdemonstratedthatthesensitivityofmixed-antigenELISAmethodwassignificantlyhigherthanthatofIFA(81.8%vs34.4%),whilethespecificityoftheformerwasdemonstratedtobe97.9%.Thecoincidenceofthede-tectionratebetweenthesetwomethodswasconsiderablyhigh,approachingupto90.0%.TheseresultssuggestthatthemixedantigenELISAassayappearstobeasensitiveandspecificmethodforsero-epide-miologicaldetectionofhumanherpesvirus8infection.

简介：尖锐吗啡管理被知道改变疱疹的功课单一的病毒感染。在这研究，潜伏的疱疹的复活上的尖锐吗啡管理的效果在一个老鼠模型被调查。因为cytolyticT淋巴细胞(CTL)的重要角色在疱疹的抑制的活动单一的病毒类型1(HSV-1)复活，CTL回答上的尖锐吗啡管理的效果也被评估。而且，淋巴细胞增长和IFN-生产在CTL反应的正式就职为他们的角色被评估。调查结果证明尖锐吗啡管理显著地减少了CTL回答，淋巴细胞增长，和IFN-生产。而且，尖锐吗啡管理被显示了重新激活潜伏的HSV-1。以前的研究证明了细胞的有免疫力的回答在HSV复活的抑制有重要角色。这些调查结果建议在尖锐吗啡管理以后的细胞的有免疫力的反应的部分的抑制可以组成导致HSV复活的机制的一部分。

简介：AbstractObjective:Human immunodeficiency virus (HIV) p24 antigen and antibody and herpes simplex virus 2 IgM are seromarkers indicating infection with HIV and herpes simplex virus-2 (HSV-2), respectively, whereas tumor necrosis factor α is an inflammatory biomarker that can be triggered by infections. Female children of single parents are faced with many socio-economic challenges that make them vulnerable to sexual influences and prone to sexually transmitted infections. The goal of this work was to determine HIV p24 antigen/antibody, HSV-2 IgM and tumor necrosis factor-α plasma levels in adult female children living in single-parent households.Methods:In this case-control observational study, 100 adult female children living with a single parent (50 living with a single mother and 50 living with a single father; age: 18-22 years) and 100 age-matched women living with both parents were recruited to serve as the test and control groups, respectively. All subjects were negative for acid-fast bacilli, plasmodium, hepatitis C virus, and hepatitis B virus. Human tumor necrosis factor α, HSV-2 IgM, antibody to hepatitis C virus, hepatitis B surface antigen and human immunodeficiency virus p24 antigen and antibody (HIV p24 Ag/Ab) levels were determined by ELISA, while the detection of acid-fast bacilli in sputum and Plasmodium in blood was carried out by optical microscopy. This work was carried out in the Owo/Ose Federal Constituency in Ondo State that shares boundaries with Edo State. The study protocol was approved by the Research and Ethical Committee of the Department of Medical Laboratory Science, Achievers University, Owo, Nigeria (AUO/MLS/2020/127) on August 27, 2020.Results:HIV p24 Ag/Ab was detected in 0 adult female children living with a single mother, 1 (2%) adult female child living with a single father and 1 (1 %) adult female child living with both parents. HSV-2 IgM was detected in 9 (18%) adult female children living with a single mother, 13 (26%) adult female children living with a single father, and 5 (10%) adult female children living with both parents.Conclusion:This work shows that adult female children of single parents are vulnerable to sexual influences, and thereby more prone to HSV-2 and possibly HIV, especially adult female children of single fathers.