简介：AIM:Toconstructfunctionalhumanfull-thicknesscornealreplacements.METHODS:Acellularporcinecornealmatrix(APCM)wasdevelopedfromporcinecorneabydecellulariztion.Thebiomechanicalpropertiesofanterior-APCM(AAPCM)andposterior-APCM(PAPCM)werecheckedusinguniaxialtensiletesting.Humancornealcellswereobtainedbycellculture.Suspendingringwasdesignedbydeformationofanacupunctureneedle.MTTcytotoxicityassaywasusedtocheckthecytotoxicityofsuspendingringsoakingsolutions.Anewthree-dimensionalorganculturesystemwasestablishedbycombinationofsuspendingring,48-wellplateandmediumtogether.Ahumanfull-thicknesscornealsubstitutewasconstructedfromhumancornealcellswithAAPCMinanorgancoculturesystem.Biochemicalmarkerexpressionoftheconstructwasmeasuredbyimmunofluorescentstainingandmorphologicalstructureswereobservedusingscanningelectronmicroscopy.Pumpfunctionandbiophysicalpropertieswereexaminedbypenetratingkeratoplastyandfollow-upclinicalobservations.RESULTS:TherewerenocellsintheAAPCMorPAPCM,whereascollagenfibers,Bowman’smembrane,andDescemet’smembranewereretained.ThebiomechanicalpropertyofAAPCMwasbetterthanPAPCM.HumancornealcellsgrewbetterontheAAPCMthanonthePAPCM.Therewasnocytotoxicityforthesuspendingringsoakingsolutions.Fortheconstructedfull-depthhumancornealreplacementskeratocytesscattereduniformlythroughouttheAAPCMandexpressedvimentin.TheepitheliallayerwaslocatedonthesurfaceofBowman’smembraneandcomposedofthreeorfourlayersofepithelialcellsexpressingcytokeratin3.OnelayerofendothelialcellscoveredthestromalsurfaceofAAPCM,expressedNa+/K+ATPaseandformedtheendotheliallayer.Theconstructwassimilartonormalhumancorneas,withmanymicrovilliontheepithelialcellsurface,stromalcellswithalongshuttleshape,andzonulaoccludensontheinterfaceofendothelialcells.Theconstructwithstoodsurgicalprocedu

简介：AIMTo与人的角膜的上皮的房间和成纤维细胞调查角膜的前面的薄片状的重建的可行性，并且在vitro.METHODSThe支架的一个非细胞组成的猪的角膜矩阵(APCM)从与0.5%钠dodecyl硫酸盐(SDS)被对待的新鲜猪的角膜被准备答案和角膜的房间的完全的移动被hematoxylin曙红证实(他)染色并且4′；，染色的6-diamidino-2-phenylindole(DAPI)。人的角膜的成纤维细胞和上皮的房间与沥滤是有教养的从APCM提取的液体，然后房间proliferative能力被MTT评估试金。构造人的角膜的前面的薄片状的代替，角膜的成纤维细胞被注入APCM并且为3d有教养，由culturing列在后面在基质构造的角膜的上皮的房间为另一10d出现。角膜的代替被分析由他染色，并且immunofluorescencestaining.RESULTSHistological检查显示在APCM由没有房间他染色，并且染色的DAPI没检测任何剩余DNA。从APCM的沥滤的液体几乎没在人的角膜的成纤维细胞和上皮的房间的增长能力上有小影响。在10d，一连续盖住APCM的表面的人的角膜的上皮的房间的3～5层被观察，并且注射角膜的成纤维细胞在支架以内散布了。构造的显型类似于正常人的角膜，与cytokeratin的高表示12在上皮的房间层和在stroma.CONCLUSIONCorneal的vimentin的高表示，前面的薄片状的代替能被与一个非细胞组成的猪的角膜矩阵栽培人的角膜的上皮的房间和成纤维细胞在vitro重建。这在vivo为进一步的移植打了基础。

简介：Thecorneahasuniquefeaturesthatmakeitausefulmodelforregenerativemedicinestudies.Itisanavascular,transparent,denselyinnervatedtissueandanypathologicalchangescanbeeasilydetectedbyslitlampexamination.Cornealsensitivityisprovidedbytheophthalmicbranchofthetrigeminalnervethatelicitsprotectivereflexessuchasblinkingandtearingandexertstrophicsupportbyreleasingneuromediatorsandgrowthfactors.Cornealnervesareeasilyevaluatedforbothfunctionandmorphologyusingstandardinstrumentssuchascornealesthesiometerandinvivoconfocalmicroscope.Alllocalandsystemicconditionsthatareassociatedwithdamageofthetrigeminalnervecausethedevelopmentofneurotrophickeratitis,araredegenerativedisease.Neurotrophickeratitisischaracterizedbyimpairmentofcornealsensitivityassociatedwithdevelopmentofpersistentepithelialdefectsthatmayprogresstocornealulcer,meltingandperforation.Currentneurotrophickeratitistreatmentsaimatsupportingcornealhealingandpreventingprogressionofcornealdamage.Novelcompoundsabletostimulatecornealnerverecoveryareinadvanceddevelopmentstage.Amongthem,nervegrowthfactoreyedropsshowedtobesafeandeffectiveinstimulatingcornealhealingandimprovingcornealsensitivityinpatientswithneurotrophickeratitis.Neurotrophickeratitisrepresentsanusefulmodeltoevaluateinclinicalpracticenovelneuro-regenerativedrugs.

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简介：·AIM:Toevaluateandcompareaspherictoricintraocularlens(IOL)implantationandasphericmonofocalIOLimplantationwithlimbalrelaxingincisions(LRI)tomanagelowcornealastigmatism(1.0-2.0D)incataractsurgery.·METHODS:Aprospectiverandomizedcomparativeclinicalstudywasperformed.Therewererandomlyrecruited102eyes(102patients)withcataractsassociatedwithcornealastigmatismanddividedintotwogroups.ThefirstgroupreceivedtoricIOLimplantationandthesecondonemonofocalIOLimplantationwithperipheralcornealrelaxingincisions.Outcomesconsideredwere:visualacuity,postoperativeresidualastigmatism,endothelialcellcount,theneedforspectacles,andpatientsatisfaction.Todeterminethepostoperativetoricaxis,allpatientswhounderwentthetoricIOLimplantationwerefurtherevaluatedusinganOPDScanIII(NidekCo,Japan).Follow-uplasted6mo.·RESULTS:Themeanuncorrecteddistancevisualacuity(UCVA)andthebestcorrectedvisualacuity(BCVA)demonstratedstatisticallysignificantimprovementaftersurgeryinbothgroups.Attheendofthefollow-uptheUCVAwasstatisticallybetterinthepatientswithtoricIOLimplantscomparedtothosepatientswhounderwentimplantationofmonofocalIOLplusLRI.Themeanresidualrefractiveastigmatismwasof0.4DforthetoricIOLgroupand1.1DfortheLRIgroup(P<0.01).Nodifferencewasobservedinthepostoperativeendothelialcellcountbetweenthetwogroups.·CONCLUSION:Thetwosurgicalproceduresdemonstratedasignificantdecreaseinrefractiveastigmatism.ToricIOLimplantationwasmoreeffectiveandpredictablecomparedtothelimbalrelaxingincision.

简介：AIM:Tostudytheeffectsofdifferentflapsizesonvisualacuity,refractiveoutcomes,andaberrationsafterfemtosecondlaserforlaserkeratomileusis(LASIK).·METHODS:Ineachofthefortypatientsenrolled,1eyewasrandomlyassignedtoreceivetreatmentwitha8.1mmdiametercornealflap,definedasthesmallflap,whiletheothereyewastreatedwitha8.6mmdiametercornealflap,definedasthebigflap.Refractiveerrors,visualacuity,andhigher-orderaberrationswerecomparedbetweenthetwogroupsatweek1,month1and3postoperatively.·RESULTS:Thepostoperativerefractiveerrorsandvisualacuityallconformedtotheintendedgoal.Postoperativehigher-orderaberrationswereincreased,especiallyinsphericalaberration(Z12)andverticalcoma(Z7).Therewerenostatisticallysignificantdifferencesbetweenthetwogroupsintermsofpostoperativerefractiveerrors,visualacuity,rootmeansquareoftotalHOAs(HO-RMS),trefoil30°(Z6),verticalcoma(Z7),horizontalcoma(Z8),trefoil0°(Z9),andsphericalaberration(Z12)atanypointduringthepostoperativefollow-up.·CONCLUSION:Boththesmallandbigflapsaresafeandeffectiveprocedurestocorrectmyopia,providedtheexposurestromameetstheexcimerlaserablations.Thepersonalizedsizecornealflapisfeasible,aswecandesignthesizeofcornealflapbasedontheprinciplethatthecornealflapdiametershouldbeequaltoorgreaterthanthesumofthemaximumablationdiameterandapparatuserror.

简介：Rabbitlimbalcornealepithelialcells,cornealendothelialcellsandkeratocyteswereculturedonamnioticmembrane.Phasecontrastmicroscopeexaminationwasperformeddaily.Histologicalandscanelectronmicroscopicexaminationswerecarriedouttoobservethegrowth,arrangementandadhesionofcultivatedcells.Resultsshowedthatthreecornealcelltypesseededonamnioticmembranegrewwellandhadnormalcellmorphology.Culturedcellsattachedfirmlyonthesurfaceofamnioticmembrane.Cornealepithelialcellsshowedsingularlayerorstratification.Cellboundarieswereformedandtightlyopposed.Cornealendothelialcellsshowedcobblestoneorpolygonalmorphologiccharacteristicsthatappeareduniforminsize.Thecellulararrangementwascompact.Keratocyteselongatedandshowedtriangleordendriticmorphologywithmanyintercellularjointswhichcouldformnetworks.Inconclusion,amnioticmembranehasgoodscaffoldproperty,diffusioneffectandcompatibilitywithcornealcells.Thebasementmembranesideofamnioticmembranefacilitatedthegrowthofcornealepithelialcellsandendothelialcellsandcelljunctionsweretightlydeveloped.Thespongylayerofamnioticmembranefacilitatedthegrowthofkeratocytesandintercellularjointswererich.Amnioticmembraneisanidealbiomaterialforlayeringtissueengineeredcornea.

简介：在所有成年体的干细胞之中，角膜的上皮的那些在他们在定义limbal结构的独占的地点是唯一的沃格特的称为的栅。作为结果，limbal的外科的嫁接上皮的干细胞与或没有前vivo扩大长被练习了在给予limbal的病人恢复风景干细胞缺乏。与另外的干细胞例子相比，不过，相对，很少对limbalniche被知道，它被相信在调整自强和命运决定oflimbal起一个枢轴的作用上皮的干细胞。这评论总结相关文学并且提出几个关键问题由集中于limbal壁龛指导未来研究进limbal干细胞缺乏和角膜的上皮的织物工程的进一步的改进的致病的更好的理解。

简介：AIMTo在角膜的endothelial房间(CEC)并且到的增长上探索调节媒介的效果比较不同调节媒介(厘米)的效率.METHODSRatCEC，角膜的stromal房间(CSC)，骨头导出髓的endothelial祖先房间(BEPC)，并且骨头导出髓的间充质的干细胞(BMSC)被孤立并且在vitro有教养。厘米从CSC，BEPC，和BMSC被收集。CEC在不同文化媒介被栽培。房间形态学被记录，并且基因和蛋白质表示是为5d在厘米种的analyzed.RESULTSAfter，在每个试验性的组的CEC仍然保持多角形，在像鹅卵石的单层安排。Immunocytofluorescence揭示了Na+/K+-ATP,aquaporin的积极表示1(AQP1)，并且zonulaoccludens1(ZO-1)。把分析基于量的聚合酶链反应(qPCR)，在CSC厘米的Na+/K+-ATP表示是尤其是由1.3褶层的upregulated(±；0.036)(P<；0.05，n=3)。ZO-1的表示层次，神经原特定的enolase(NSE)，Vimentin，配对的homebox6(PAX6)，并且procollagen类型VIII(COL8A1)是尤其是在每个试验性的组的upregulated。每厘米在CEC增长上有积极效果，并且CSC厘米在proliferation.CONCLUSIONCSC厘米，BEPC厘米，和BMSC厘米上有最强壮的效果不仅刺激了CEC的增长，而且坚持说特征区分了为endothelial功能必要的显型。CSC厘米在CEC增长上有最著名的效果。

简介：AIM:TodescribetheprevalenceanddemographiccharacteristicsofcornealblindnessinanurbanandruralregionofNingxia,locatedinthenorthwestpartofChina.METHODS:Astratified,randomizedsamplingprocedurewasemployedinthestudy,includingurbanandruralareaofallagegroup.Visualacuity,anteriorsegmentandocularfunduswerechecked.Relatedfactorofcornealdisease,includingage,gender,educationstatus,ethnicgroup,locationandoccupation,wereidentifiedaccordingtouniformcustomizedprotocol.Aneyewasdefinedtobecornealblindnessifthevisualacuitywas<20/400duetoacornealdisease.RESULTS:Threethousandindividuals(1290fromurbanareaand1710fromruralarea)participatedintheinvestigation,witharesponserateof80.380%.Theprevalenceofcornealblindnesswas0.023%inbotheyesand0.733%inatleastoneeye.Theblindnessinatleastoneeyewithvariedcauseswaspresentin106participants(3.533%)andinbilateraleyesin34participants(1.133%).Thecornealdiseasesaccountedfor20.754%ofblindnessinatleastoneeyeand20.588%ofbilateralblindness.TheprevalenceofcornealdiseasewashigherinolderandHanethnicgroup,especiallythosewhooccupiedinagricultureandoutdoorwork.Peoplewithcornealblindnessweremorelikelytobeolderandlowereducation.Ruralpopulationweremorelikelytosufferfrombilateralcornealblindnessthantheurbanpopulationin≥59-yeargroup(χ2=6.716,P=0.019).Infectious,traumaandimmunecornealdiseasewerethethreeleadingcausesofcornealdisease.Traumacornealdiseasewasmorelikelyleadingtoblindnessinoneeye.However,infectiousandimmunecornealdiseasesmakemorecontributiontothebilateralcornealblindness.CONCLUSION:CornealblindnessisasignificantburdenofinNingxiapopulation,encompassingavarietyofcornealinfectionsandtrauma;themajorityofthosewereavoidable.Healthpromotionstrategiesandgoodhygienicconditionshavetobedeveloped.

简介：这研究试图评估结果并且描述了cryopreservedlimbal的恢复过程为角膜的外设和limbal疾病的薄片状的keratoplasty(CLLK)。有一个平均数的12个病人的13只眼睛41变老

简介：AIM:Totransfectthecatcornealendothelialcells(CECs)withrecombinanthumanβ-nervegrowthfactorgeneadeno-associatedvirus(AAV-β-NGF)andtoobservetheeffectoftheexpressedβ-NGFproteinontheproliferationactivityofcatCECs.METHODS:TheendotheliumofcatcorneawastornunderthemicroscopeandrapidlycultivatedinDulbecco’smodifiedEagle’smedium(DMEM)toformsinglelayerCECsandthepassage2endothelialcellswereusedinthisexperiment.TherecombinanthumanAAV-β-NGFwasconstructed.TherecombinanthumanAAV-β-NGFwastransferredintocatCECsdirectly.Threegroupswereasfollowing:normalCECcontrolgroup,CEC-AAVcontrolgroupandrecombinantCECAAV-β-NGFgroup.Forty-eighthoursaftertransfection,thetotalRNAwasextractedfromtheCECbyTrizol.Theexpressionoftheβ-NGFtargetgenedetectedbyfluorescencequantitativepolymerasechainreaction;proliferationactivityofthetransfectedCECdetectedat48hbyMTTassay;thepercentageofG1cellsamongCECsaftertransfectwasdetectedbyflowcytometrymethod(FCM);cellmorphologywasobservedunderinvertedphasecontrastmicroscope.RESULTS:Thetornendotheliumculturetechniquerapidlycultivatedsinglelayercatcornealendothelialcells.Theself-designedprimersforthetargetgeneandreferencegenewereefficientandspecialconfirmedthroughelectrophoresisanalysisandDNAsequencing.Forty-eighthoursaftertransfect,thehumanβ-NGFgenemRNAdetectedbyfluorescencequantitativepolymerasechainreactionshowedthattherewasnosignificantdifferencebetweennormalCECcontrolgroupandCECAAVcontrolgroup(P>0.05);therewassignificantdifferencebetweentwocontrolgroupsandrecombinantCEC-AAV-β-NGFgroup(P<0.05).MTTassayshowedthattransfectofrecombinantAAV-β-NGFpromotedtheproliferationactivityofcatCEC,whiletherewasnosignificantdifferencebetweennormalCECcontrolgroupandCEC-AAVcontrolgroup(P>0.05).FCMresultshowedthatthepercentageofG1cellsinCECAAV-NGFgroupwas76.8%whi

简介：AIM:Tocomparethecornealparametersofchildrenwithcongenitalisolatedgrowthhormonedeficiencyandhealthysubjects.METHODS:Inthiscross-sectional,prospectivestudy,50caseswithgrowthhormone(GH)deficiencytreatedwithrecombinantGHand71healthychildrenunderwentacompleteophthalmicexamination.Thecornealhysteresis(CH),cornealresistancefactor(CRF),Goldmann-correlatedintraocularpressure(IOPg)andcorneal-compensatedintraocularpressure(IOPcc)weremeasuredwiththeOcularResponseAnalyzer(ORA).Centralcornealthickness(CCT)wasmeasuredbyaultrasonicpachymeter.RESULTS:Themeanagewas13.0±3.0yearsintheGHdeficiencygroupconsistingof21femalesand29malesand13.4±2.4yearsinthehealthychildrengroupconsistingof41femalesand30males.Therewasnostatisticallysignificantdifferencebetweenthegroupsforgenderorage(Chi-squaretest,P=0.09;independentttest,P=0.28,respectively).ThemeandurationofrecombinantGHtherapywas3.8±2.4yinthestudygroup.ThemeanCH,CRF,IOPgandIOPccvalueswere11.0±2.0,10.9±1.9,15.1±3.3,and15.1±3.2mmHgrespectivelyinthestudygroup.Thesamevalueswere10.7±1.7,10.5±1.7,15.2±3.3,and15.3±3.4mmHgrespectivelyinthecontrolgroup.ThemeanCCTvalueswere555.7±40.6,545.1±32.5μminthestudyandcontrolgroupsrespectively.TherewasnostatisticallysignificantdifferencebetweenthetwogroupsforCH,CRF,IOPg,IOPccmeasurementsorCCTvalues(independentt-test,P=0.315,0.286,0.145,0.747,0.13respectively).CONCLUSION:OurstudysuggeststhatGHdeficiencydoesnothaveaneffectonthecornealparametersandCCTvalues.ThisobservationcouldbebecauseofthedurationbetweenthebeginningofdiseaseandthediagnosisandbeginningofGHtherapy.

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简介：AIMTo算术地代表在角膜的几何学和intracorneal戒指的飞机植入的ectatic(ICRS)之间的交叉以便在keratoconus(KC)决定角膜的反应到ICRS外科。此后，角膜的戒指飞机交叉被建模介绍这个概念和keratectasia.METHODSThe的治疗的ICRS外科的一张最新导出的指导地形学的诺模图的早结果到圆锥形的节。戒指效果是结果：戒指尺寸，位置(陡峭对扁平)，地点(从角膜的几何中心的距离)，和在戒指弯曲和隧道弯曲之间的差异。Femtosecond激光被用来创造隧道，切口地点根据诺模图被选择以便不管圆锥形的节有的refraction.RESULTSThe的散光轴把最厚的戒指放在角膜的最陡峭的部分在角膜的陡峭的区域的更延长的形状比在里面扁平的区域，取决于角膜的sagittal弯曲。相等的戒指尺寸比在扁平的区域在陡峭的区域有更多的变平的效果。厚片断应该不管折射的柱体轴在角膜的陡峭的部分下面被植入。在陡峭的区域的单个片断在KC的早、中等的盒子中是足够的。新诺模图比常规nomogram.CONCLUSIONThe最新发源的在未改正的视觉尖酸(UCVA)和改正最好的视觉尖酸(BCVA)向更多的地志的整齐提供了散光和更好的改进的重要减小诺模图能比常规诺模图生产更好的结果。而且基于这个概念，一张新诺模图能集成于与允许的修改焦点的asphericity创造指导地形学的、设定的、椭圆的隧道的激光软件设定不规则地变得陡峭的ectatic角膜的焦点的变平的femtosecond。

简介：Objective:Toobservetheocularhistopathologicalchangesaftereyeballenucleationinducedbycornealtrauma.Methods:Lightmicroscopicexaminationwasdoneon117eyeballspecimensenucleatedaftercornealtrauma(18withcornealfissureand99withcornealperforatingtrauma).Results:Acute,subacuteorchronicinflammatorychanges,andfibrousmembraneformationwereobservedinwell-closedcornealwounds,whereasinflammation,atrophyandscarwereobservedinthefocaltissues.Butatthelateperiod,secondaryglaucoma,retinaldetachment,endophthalmitisandeyeballatrophyresultedinblindness.Cornealfistulawasobservedinthosewithinadequatecureofwoundscausedbyingrowthofcornealepithelium,embedmentofirisandvitreousbody,andlargeareaofcentrallylocatedtissuedeficiencyofthecorneal.Ahighincidenceofendophthalmitiswasnotedduetothepresenceofcornealfistula.Severeinflammationwasobservedintheanteriorsegmentaltissueswithfibrousinfiltrationintheanteriorchamber,whichmightresultinrapiddestructionoftheeyeballs.Conclusions:Ocularpathologyvarieswiththedifferenceoftheposition,form,sizeandclosingconditionsofthecorneallacerationaftertrauma.

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简介：AIM:Toevaluatetheinterchangeabilityofkeratometricandasphericitymeasurementsprovidedbythreemeasurementsystemsbasedondifferentopticalprinciples.METHODS:Atotalof40eyesof40patientswithameanageof34.1ywereincluded.Inallcases,acornealcurvatureanalysiswasperformedwithIOL-Master(IOLM),iDesign2(ID2),andSiriussystems(SIR).Differencesbetweeninstrumentsforflattest(K1)andsteepest(K2)keratometricreadings,aswellasformagnitudeandaxisofcornealastigmatismwereanalyzed.Likewise,differencesinasphericity(Q)betweenSIRandID2werealsoevaluated.RESULTS:MeandifferencesbetweendevicesforK1were0.20±0.21(P<0.001),-0.12±0.36(P=0.046)and-0.32±0.36D(P<0.001)forthecomparisonsIOLM-SIR,IOLM-ID2andSIR-ID2,respectively.Therangesofagreementforthesecomparisonsbetweeninstrumentswere0.41,0.70,and0.70D.ForK2,meandifferenceswere0.31±0.33(P<0.001),-0.08±0.43(P=0.265)and-0.39±0.38D(P<0.001),withrangesofagreementof0.65,0.84,and0.74D.Concerningmagnitudeofastigmatism,rangesofagreementwereinthelimitofclinicalrelevance(0.49D,P=0.011;0.55D,P=0.386;0.43D,P=0.05).Incontrast,rangesofagreementwereclinicallyrelevantforastigmaticaxis(26.68o,33.83oand18.37o,P≥0.121)andforQbetweenSIRandID2(0.16,P<0.001).CONCLUSION:Thekeratometriccornealpower,astigmaticaxisandasphericitymeasurementsprovidebythethreesystemsevaluatedcannotbeconsideredasinterchangeable,whereasmeasurementsofcornealastigmatismobtainedwithSIRandID2canbeconsideredasinterchangeableforclinicalpurposes.

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