简介：Rabbitlimbalcornealepithelialcells,cornealendothelialcellsandkeratocyteswereculturedonamnioticmembrane.Phasecontrastmicroscopeexaminationwasperformeddaily.Histologicalandscanelectronmicroscopicexaminationswerecarriedouttoobservethegrowth,arrangementandadhesionofcultivatedcells.Resultsshowedthatthreecornealcelltypesseededonamnioticmembranegrewwellandhadnormalcellmorphology.Culturedcellsattachedfirmlyonthesurfaceofamnioticmembrane.Cornealepithelialcellsshowedsingularlayerorstratification.Cellboundarieswereformedandtightlyopposed.Cornealendothelialcellsshowedcobblestoneorpolygonalmorphologiccharacteristicsthatappeareduniforminsize.Thecellulararrangementwascompact.Keratocyteselongatedandshowedtriangleordendriticmorphologywithmanyintercellularjointswhichcouldformnetworks.Inconclusion,amnioticmembranehasgoodscaffoldproperty,diffusioneffectandcompatibilitywithcornealcells.Thebasementmembranesideofamnioticmembranefacilitatedthegrowthofcornealepithelialcellsandendothelialcellsandcelljunctionsweretightlydeveloped.Thespongylayerofamnioticmembranefacilitatedthegrowthofkeratocytesandintercellularjointswererich.Amnioticmembraneisanidealbiomaterialforlayeringtissueengineeredcornea.

简介：Objective:Toobservetheocularhistopathologicalchangesaftereyeballenucleationinducedbycornealtrauma.Methods:Lightmicroscopicexaminationwasdoneon117eyeballspecimensenucleatedaftercornealtrauma(18withcornealfissureand99withcornealperforatingtrauma).Results:Acute,subacuteorchronicinflammatorychanges,andfibrousmembraneformationwereobservedinwell-closedcornealwounds,whereasinflammation,atrophyandscarwereobservedinthefocaltissues.Butatthelateperiod,secondaryglaucoma,retinaldetachment,endophthalmitisandeyeballatrophyresultedinblindness.Cornealfistulawasobservedinthosewithinadequatecureofwoundscausedbyingrowthofcornealepithelium,embedmentofirisandvitreousbody,andlargeareaofcentrallylocatedtissuedeficiencyofthecorneal.Ahighincidenceofendophthalmitiswasnotedduetothepresenceofcornealfistula.Severeinflammationwasobservedintheanteriorsegmentaltissueswithfibrousinfiltrationintheanteriorchamber,whichmightresultinrapiddestructionoftheeyeballs.Conclusions:Ocularpathologyvarieswiththedifferenceoftheposition,form,sizeandclosingconditionsofthecorneallacerationaftertrauma.

简介：Theobjectiveofthisstudywastoproducetheporouscollagen-chitosan/Glycosanminglycans(GAG)forcornealceil-seedimplantasathree-dimensionaltissueengineeringscaffoldtoimprovetheregenerationcorneas.Theeffectofvariouscontentofglycerolasformporousagenttocollagen-chitosan/GAGpreservedaporousdimensionalstructurewasinvestigated.Theheat-dryingwasusedtoprepareporouscollagen-chitosan/GAGscaffold.Theporemorphologyofcollagenchitosan/GAGwascontrolledbychangingtheconcentrationofglycerolsolutionanddryingmethods.TheporousstructuremorphologywasobservedbySEM.Thediameteroftheporesform10to50μm.Thehighlyporousscaffoldhadinterconnectingpores.Thecornealcellmorphologywasobservedunderthelightmicroscope.Theseresultssuggestthatcollagen-chitosan/GAGshowedthatcornealcellhaveformedconfluentlayersandresemblethesurfaceofnormalcornealcellsurface.