简介:摘要目的对猪链球菌2型菌株98HAH33基因0267进行功能研究。方法比较猪链球菌2型野生株98HAH33、突变株Δ0267及回复株CΔ0267在不同生长时期的生长速率,细菌黏附、全血杀伤及巨噬细胞吞噬试验比较对宿主的黏附和抗吞噬能力,仔猪试验比较在毒力方面的差异。结果野生株、突变株及回复株生长速率一致;野生株对宿主细胞A549、Hep2、HBMEC的黏附率分别是突变株的2.59、4.87和3.08倍,回复株对宿主细胞A549、Hep2、HBMEC的黏附率分别是突变株的2.65、4.65和2.86倍;野生株、突变株和回复株1~3 h在全血中的存活率分别是36.91%、28.12%和41.61%,58.44%、44.06%和58.26%,93.02%、70.08%和95.85%;小鼠巨噬细胞RAW264.7吞噬试验结果显示RAW264.7吞噬野生株、突变株及回复株的菌量分别为2 767、5 322、2 567;仔猪竞争感染试验证明基因0267与猪链球菌2型98HAH33的毒力相关。结论猪链球菌2型98HAH33基因0267与细菌黏附和抗吞噬相关,是一个新毒力因子。
简介:摘 要 :本文以石头河区域鹦鸽站为例,对 SSXT-SQ-02型土壤水分监测仪与人工烘干法的同步监测资料进行应用分析,结果表明:土层深度 10cm绝对误差的平均值 δ为 0.753%, 20cm绝对误差的平均值 δ为 0.878%, 10cm绝对误差的平均值 δ为 0.753%,平均绝对误差的平均值 δ为 0.795%均小于实验室条件下为 δ≤2%,野外条件 δ≤4%,符合规范要求。表明 SSXT-SQ-02型土壤水分监测仪可为土壤墒情监测工作提供有效的技术支撑,可在生产中的到应用。
简介:摘要目的探讨亚砷酸钠(NaAsO2)通过活性氧(ROS)蓄积及线粒体损伤诱导人肝细胞L-02凋亡的作用,为砷中毒的作用机制研究提供实验依据。方法体外培养L-02细胞,分为对照组、NaAsO2组(10 μmol/L NaAsO2)、N-乙酰半胱氨酸(NAC)组(5 mmol/L NAC)、NaAsO2 + NAC组(10 μmol/L NaAsO2、5 mmol/L NAC),培养24 h。分别用二氯二氢荧光素-乙酰乙酸酯(DCFH-DA)荧光探针法、JC-1染色法、硫氰酸荧光素/碘化丙啶(Annexin V-FITC/PI)双染法检测细胞内ROS水平、线粒体膜电位去极化比例和细胞凋亡率;用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹法(Western blot)检测半胱氨酸天冬氨酸蛋白酶3(Caspase3)、细胞色素C蛋白(Cyt-C)、线粒体DNA编码的细胞色素C氧化酶Ⅳ(COXⅣ)mRNA、蛋白表达水平。结果各组间细胞内ROS水平(3 857 392.33 ± 44 928.39、4 515 288.00 ± 32 660.64、3 670 150.67 ± 101 987.69、4 035 235.67 ± 99 995.30),线粒体膜电位去极化比例(2.16 ± 0.54、7.95 ± 0.52、2.70 ± 0.29、1.01 ± 0.23),细胞总凋亡率(1.45 ± 0.03、4.27 ± 0.17、1.87 ± 0.12、2.52 ± 0.35)比较差异有统计学意义(F = 62.62、159.81、112.70,P均< 0.05);各组间Caspase3、Cyt-C、COXⅣ mRNA表达水平比较差异有统计学意义(F = 9.20、7.33、14.87,P均< 0.05);各组间活化Caspase3(cleaved-Caspase3)、Cyt-C、COXⅣ蛋白表达水平比较差异有统计学意义(F = 31.42、8.01、83.30,P均< 0.05)。与对照组比较,NaAsO2组L-02细胞内ROS水平、线粒体膜电位去极化比例、细胞总凋亡率明显增高(P均< 0.05);Caspase3、Cyt-C mRNA和cleaved-Caspase3、Cyt-C蛋白表达水平明显增高(P均< 0.05),COXⅣ mRNA和蛋白表达水平则显著降低(P均< 0.05)。与NaAsO2组比较,NaAsO2 + NAC组细胞内ROS水平、线粒体膜电位去极化比例、细胞总凋亡率明显下降(P均< 0.05);Caspase3 mRNA和cleaved-Caspase3蛋白表达水平明显降低(P均< 0.05),Cyt-C mRNA和蛋白表达水平明显降低(P均< 0.05),COXⅣ mRNA和蛋白表达水平明显增高(P均< 0.05)。结论NaAsO2可刺激L-02细胞产生大量ROS,诱发线粒体去极化,引发线粒体损伤,使Cyt-C向线粒体外释放增加,激活线粒体凋亡途径Caspase3蛋白酶而引起L-02细胞发生凋亡,这有可能是砷致肝损伤的主要作用机制之一。
简介:[摘要]在设计工作中有时会遇到建筑物基础不在同一标高,存在高差,且工程要求先施工较浅基础,经过计算对建筑物之间的距离提出最小要求成为方案设计时结构工程师的必要工作,本文结合工程实例提出最小距离的确定过程。
简介:AbstractBackground:Epithelial to mesenchymal transition (EMT) is a key process in determining distant metastasis and intra-hepatic dissemination of hepatocellular carcinoma (HCC). Follistatin (FST) family members are considered to be an attractive therapeutic targets and prognostic indicators in cancers. As a derivative of FST, Follistatin Like 5 (FSTL5) may play a similar role in HCC cells. This study aimed to investigate the expression and function of FSTL5 in HCC and its role in EMT.Methods:FSTL5, E-cadherin and vimentin in HCC, and paracancerous tissues were detected by immunohistochemistry. Correlation of FSTL5 expression with overall survival was assessed. The proliferation and invasion of HCC cell lines SK-Hep1 and MHCC-LM3 were analyzed by cell counting kit-8 and Transwell assays. The expression of FSTL5, E-cadherin, and vimentin in HCC cells was examined by polymerase chain reaction and Western blot analysis. T-test was used to analyze the difference in proliferation and invasion ability between groups. The Spearman rank correlation test was used to detect the correlation between the expression of FSTL5 and E-cadherin or vimentin.Results:The expression of FSTL5 in HCC was lower than that in paracancerous tissues (9.97% vs. 82.55%, χ2 = 340.15, P < 0.001). Patients with high FSTL5 expression had a better prognosis (χ2= 8.22, P= 0.004) and smaller tumor diameter (χ2 = 45.52, P < 0.001), less lymph node metastasis (χ2 = 5.58, P= 0.02), earlier tumor node metastasis stage (χ2 = 11.29, P= 0.001), a reduced number of tumors (χ2 = 5.05, P= 0.02), lower alpha-fetoprotein value (χ2 = 24.36, P < 0.001), more probability of hepatitis carrying (χ2 = 40.9, P < 0.001), and better liver function grade (χ2 = 5.21, P = 0.02). Immunohistochemistry showed that FSTL5 expression in HCC tissues was positively correlated with E-cadherin expression (r = 0.38, P < 0.001) and negatively correlated with vimentin expression (r = -0.385, P < 0.001). Furthermore, over-expression of FSTL5 up-regulated the expression of E-cadherin and down-regulated the expression of vimentin in SK-Hep1 (negative control [NC] vs. FSTL5-interfering group [Lv-FSTL5]: E-cadherin [t= 45.03, P < 0.001], vimentin [t= 67, P < 0.001]) and MHCC-LM3 (NC vs. Lv-FSTL5: E-cadherin [t = 50, P < 0.001], vimentin [t = 72.75, P < 0.001]) cells at mRNA level. The same as protein level. In addition, the over-expression of FSTL5 inhibited the proliferation (NC vs. Lv-FSTL5: SK-Hep1, 3 d [t = 7.324, P = 0.018], 4 d [t = 6.23, P = 0.021], 5 d [t= 10.21, P= 0.003]; MHCC-LM3, 3 d [t= 4.32, P= 0.037], 4 d [t= 7.49, P= 0.012], 5 d [t= 9.3661, P = 0.009]) and invasion (NC vs. Lv-FSTL5: SK-Hep1, t= 21.57, P < 0.001; MHCC-LM3, t= 18.04, P < 0.001) of HCC cells.Conclusions:Down-regulation of FSTL5 may contribute to EMT of HCC, and FSTL5 is a potential target in the treatment of HCC.
简介:摘要目的探究重组人促血小板生成素(rhTPO)对健康人肝细胞株L02的影响。方法以健康人肝细胞株L02为研究对象,通过不同剂量rhTPO[对照组(0 U rhTPO)、低剂量组(150 U rhTPO)及高剂量组(750 U rhTPO)]处理L02细胞,观察各组L02细胞的存活率。采用Western-blotting检测各组细胞[A组(0 U rhTPO)、B组(15 U rhTPO)、C组(150 U rhTPO)及D组(1 500 U rhTPO)]的细胞外信号调节激酶(ERK)及其磷酸化产物(p-ERK)蛋白的表达水平,并采用荧光定量PCR检测ERK信使RNA(mRNA)表达水平。结果各组L02细胞存活率的比较,差异有统计学意义(F = 8.843,P = 0.016)。进一步两两比较发现,低剂量组及高剂量组的存活率均显著高于对照组[(107.9 ± 4.0)%、(112.0± 1.6)%、(100.0 ± 2.5)%],且高剂量组细胞的存活率更高(P均< 0.05)。各组L02细胞间ERK蛋白及ERK mRNA表达水平的比较,差异均无统计学意义(F = 1.140,P = 0.392;F = 0.437,P = 0.733),而各组L02细胞间p-ERK蛋白表达水平的比较差异有统计学意义(F = 31.400,P < 0.001),且B组、C组及D组细胞的p-ERK蛋白表达水平均显著高于A组细胞(P均< 0.05),而B组、C组及D组细胞p-ERK蛋白表达水平两两比较,差异均无统计学意义(P均> 0.05)。结论rhTPO可通过激活ERK促进L02细胞数量增殖。
简介:摘要目的观察亚砷酸钠(NaAsO2)对人正常肝细胞(L-02细胞)脂代谢基因固醇调节元件结合蛋白-1c(SREBP-1c)、过氧化物酶体增殖物激活受体α(PPARα)和脂肪酸合成酶(FAS)表达的影响。方法体外培养L-02细胞,分别以0(对照)、2、4、8、16、32、64、128 μmol/L NaAsO2染砷24 h,采用CCK-8法检测细胞存活率,并制作拟合曲线计算半抑制浓度(IC50),以IC50的0、1/8、1/4、1/2作为染砷剂量进行后续实验。采用甘油磷酸氧化酶-过氧化氢酶(GPO-PAP)法检测细胞甘油三酯(TG)含量;实时荧光定量PCR检测SREBP-1c、PPARα、FAS mRNA表达水平;蛋白质印迹法(Western blot)检测SREBP-1c和PPARα蛋白表达水平。结果8、16、32、64、128 μmol/L NaAsO2组细胞存活率[(92.000 ± 1.414)%、(91.000 ± 0.000)%、(76.500 ± 0.707)%、(53.000 ± 1.412)%、(47.000 ± 1.412)%]明显低于对照组[(100.000 ± 0.000)%,P均< 0.01],IC50为64 μmol/L,以0(对照)、8、16、32 μmol/L NaAsO2进行后续实验。与对照组[(1.000 ± 0.000)mmol/g prot]比较,8、16、32 μmol/L NaAsO2组TG含量[(0.691 ± 0.064)、(0.474 ± 0.162)、(0.184 ± 0.045)mmol/g prot]显著降低(P均< 0.01)。与对照组比较,各染砷组SREBP-1c、PPARα、FAS mRNA表达水平,SREBP-1c、PPARα蛋白表达水平显著降低(P < 0.01或< 0.05)。相关性分析可见,NaAsO2含量与细胞TG含量,SREBP-1c和PPARα蛋白表达水平呈负相关(r =-0.954、- 0.875、- 0.965,P均< 0.01)。结论NaAsO2可引起L-02细胞TG含量减少及脂代谢相关基因SREBP-1c、PPARα和FAS表达降低,提示砷致肝损伤过程中可引起脂代谢障碍发生。
简介:AbstractBackground:Malaria surveillance system strengthening is essential in the progress towards malaria elimination. In Nigeria, more attention is being given to this recently as the country is striving towards achieving elimination. However, the surveillance system performance is fraught with challenges including poor data quality with varying magnitude by state. This study evaluated the operation of the Kano State malaria surveillance system and assessed its key attributes.Methods:An observational study design comprising a survey, record review and secondary data analysis, and mixed methods data collection approach were used. Four key stakeholders' and 35 Roll Back Malaria Focal Persons (RBMs) semi-structured interviews on operation of the system and attributes of the surveillance system, were conducted. We analyzed the abstracted 2013-2016 National Health Management Information System web-based malaria datasets. The surveillance system was evaluated using the "2001 United States Centers for Disease Control's updated guidelines for Evaluating Public Health Surveillance Systems". Data were described using means, standard deviation, frequencies and proportions. Chi-squared for linear trends was used.Results:Overall, 24 RBMs (68.6%) had ≤ 15-year experience on malaria surveillance, 29 (82.9%) had formal training on malaria surveillance; 32 RBMs (91.4%) reported case definitions were easy-to-use, reporting forms were easy-to-fill and data flow channels were clearly defined. Twenty-seven respondents (69.2%) reported data tools could accommodate changes and all RBMs understood malaria case definitions. All respondents (4 stakeholders and 34 RBMs [97.1%]) expressed willingness to continue using the system and 33 (84.6%) reported analyzed data were used for decision-making. Public health facilities constituted the main data source. Overall, 65.0% of funding were from partner agencies. Trend of malaria cases showed significant decline (χ2trend = 7.49; P = 0.0006). Timeliness of reporting was below the target (≥ 80%), except being 82% in 2012.Conclusions:Malaria surveillance system in Kano State was simple, flexible, acceptable, useful and donor-driven but the data were not representative of all health facilities. Timeliness of reporting was suboptimal. We recommended reporting from private health facilities, strengthening human resource capacity for supportive supervision and ensuring adequate government funding to enhance the system's representativeness and improve data quality.
简介:摘要目的探讨核因子-E2相关因子2(Nrf2)信号通路在亚砷酸钠(NaAsO2)致人正常肝细胞(L-02细胞)氧化损伤过程中的作用,为砷致肝损伤的氧化损伤作用机制研究提供实验依据。方法体外培养L-02细胞,分别以0(对照)、25、50、75、100、125、150 μmol/L NaAsO2处理细胞24 h,采用CCK8法检测细胞存活率;根据细胞存活率计算半抑制浓度(IC50),分别以IC50的0、1/8、1/4、1/2倍剂量NaAsO2处理L-02细胞进行分组实验。采用蛋白免疫印迹法(Western blot)检测L-02细胞中和细胞核内Nrf2信号通路相关因子Nrf2、血红素氧化酶-1(HO-1)、醌氧化还原酶1(NQO1)、谷胱甘肽过氧化物酶1(GPx1)的蛋白表达情况。结果CCK8实验结果显示,25、50、75、100、125、150 μmol/L NaAsO2组的L-02细胞存活率[(69.53 ± 0.06)%、(41.33 ± 0.08)%、(23.65 ± 0.04)%、(26.51 ± 0.04)%、(31.63 ± 0.01)%、(26.24 ± 0.02)%]明显低于对照组[(100.00 ± 0.00)%,P均< 0.05];细胞存活率的IC50为40 μmol/L,分组实验的NaAsO2剂量分别采用0(对照)、5、10、20 μmol/L。Western blot检测结果显示,与对照组比较,5、10、20 μmol/L NaAsO2组L-02细胞中Nrf2、HO-1及L-02细胞核内HO-1蛋白水平显著升高(P均< 0.05);10、20 μmol/L NaAsO2组L-02细胞中GPx1蛋白水平显著降低(P均< 0.05),L-02细胞核内Nrf2蛋白水平显著升高(P均< 0.05);5 μmol/L NaAsO2组L-02细胞核内NQO1蛋白水平显著升高(P < 0.05)。结论NaAsO2对L-02细胞中Nrf2信号通路相关因子的表达有影响,其致L-02细胞氧化损伤的作用机制可能与Nrf2信号通路有关。
简介:摘要:目的: 进一步对我市 市售食品致病菌污染状况进行 了解。 方法: 严格以《 食源性致病菌监测工作手册》开展 检验工作,对受检 食品均给予 沙门氏菌、 金黄色葡萄球菌等 食源性致病菌( 8种) 检测和 鉴定,从而进一步了解我市 市售食品致病菌污染状况。 结果: 此次共 抽检食品四类,仅只有 果蔬类食品没有 检出致病菌;而 致病菌检测率最高的为 生食水产品(以 副溶血性弧菌为主),其次分别为 熟制米面制品(以 蜡样芽孢杆菌为主)和 肉制品(以 单核细胞增生李斯特氏菌为主)。 结论: 我市 食品中, 被致病菌污染最严重的为 食品监督监管方面,需重点对以上 三类视频进行强化管理。
简介:摘要:目的 深入了解昭阳区的居民食用盐碘含量以及孕妇和儿童的碘营养状况,为科学补碘、合理补碘提供有力依据。方法 在 2016-2019年选取昭阳区东西南北中五个乡(镇)、街道,每年在每个乡(镇)、街道选取 20名孕妇和 40名 8-10岁的非寄宿小学生,采集其尿液样本,并且还要采集当前家中的使用盐样,对其进行检测,在此基础上深入分析居民的合格碘盐食用率、碘盐中位数等相关数据。对儿童以及孕妇的尿碘情况以及儿童的甲状腺肿大情况进行深入的分析。结果 2016年 -2019年的碘盐覆盖率均在 94%以上, 8-10岁儿童的甲肿率为 1.30%,儿童的尿碘含量偏高,孕妇处于适宜状态。结论 昭阳区的人群的碘营养情况相对来说处于较为适宜的状态,但是仍然有一部分人群的碘摄入量较高,仍需开展长期的碘缺乏病监测工作,促进健康成长和正常发育。