简介:摘要目的探索清喉消炎颗粒的最佳提取条件,为清喉消炎颗粒的制备、质量控制和临床应用提供理论依据.方法以出膏率为指标,采用正交试验法对清喉消炎颗粒提取进行优选,实验选择浸泡时间、煎煮时间、含醇量三个因素,每因素三个水平,用L9(33)正交表进行实验设计.结果清喉消炎颗粒的最佳提取工艺是加水煎煮两次,浸泡时间1??5h、煎煮时间1h和2/3h、含醇量55%.结论在优选条件下,清喉消炎颗粒出膏率高,以此条件制备清喉消炎颗粒,并对处方中主药板蓝根和岗梅根进行定性鉴定,进一步证明此提取工艺的合理可行.关键词清喉消炎颗粒;正交试验法;定性鉴定TheOrthogonalTestofXiaoYanQingHouGranulesextractionWUYue-hong,HUANGLe-shan,HUANGHan-hui,WULi-min(Dept??ofPharmaGcy,TheThirdaffiliatedHospitalofGuangzhouMedicaluniversity,Guangzhou510150,China;GuangzhouMedicalUniversity,Guangzhou510180,China)AbstractObjectiveThroughexploretheextractionprocessofXiaoYanQingHougranulestoprovideatheoreticalbasisfortheXiaoYanQingHougranulespreparation,thequalitycontrolandtheclinicalapplication??MethodsUsingtherateofextractionasindexandbasingontheorthogonaltestmethodtooptimizeXiaoYanQingHougranules’extractionprocess??TherewerethreemainfactorsintheExperimentsoakingtime,decoctingtime,alcoholcontent,andeachfacGtorhadthreelevels??TheexperimentaldesignusedL9(33)orthogonaltable??ResultsTheoptimizedextractiontechnologyfollowedasdecoctingtwotimes,soaGkingtime1??5hourdecoctingtime1hourand2/3houralcoholcontent55%??Conclusion,Undertheoptimizationconditions,XiaoYanQingHougranules’rateofextractionwashigh,toproduceXiaoYanQingHougranules,sowemakesomequalitativeidentificationofmaindrugsintheprescriptionofRadixIsatidisandGangKMeeyigweonrtdosprovethisprocessarerationalityandfeasibility??XiaoYanQingHougranules;Orthogonaltest;Qualitativeidentification中图分类号R291文献标识码A文章编号1008-6315(2015)12-0612-02