简介:摘要目的探讨葫芦岛市巨大儿发生率与母亲孕期体重增长的关系。方法对2009年1月-2010年12月间于我院产检并分娩的足月单胎初产孕妇587例进行调查。专人询问孕妇孕前身高、体重,计算孕前体重指数,分娩前一周内测量体重,计算整个孕期体重增长,并记录新生儿出生体重。按照孕前体重指数将研究对象分为低体重组、正常体重组、超重及肥胖组三组,分析各组巨大儿发生率与母亲孕期体重增长之间的关系。结果孕前超重及肥胖组的巨大儿发生率高于孕前正常体重组,随着孕期体重增长的升高,三组的巨大儿发生率均呈升高趋势。结论随着孕期体重增长的升高,巨大儿的发生率呈升高趋势。
简介:摘要目的通过分析孕期不同阶段体重增长情况对新生儿出生体重的影响,指导孕妇在妊娠的不同阶段通过合理调整饮食和运动控制新生儿出生体重,从而降低围产期母儿患病率和死亡率。方法本文对五个不同新生儿体重组共251例孕妇的孕前、产前体重及同孕期体重增加情况的统计及其与新生儿体重的相关性分析。结果孕妇孕前及产前体重对新生儿体重影响不明显;孕20周前体重增长缓慢者出生低体重儿的几率增大;孕中晚期体重增加过多的孕妇,出生巨大儿几率增大;孕20-34周的体重变化对新生儿出生体重影响最大。结论认真观察和和合理的饮食指导,控制妊娠20-34周体重变化,对控制孕妇和新生儿体重有指导意义。
简介:AIM:Toinvestigatewhether15-Lipoxygenase-1(15-LOX-1)playsanimportantroleintheregulationofangiogenesis,inhibitinghypoxia-inducedproliferationofretinalmicrovascularendothelialcells(RMVECs)andtheunderlyingmechanism.METHODS:PrimaryRMVECswereisolatedfromtheretinasofC57/BL6JmiceandidentifiedbyanevaluationforFITC-markedCD31.ThehypoxiamodelswereestablishedwiththeBio-bagandevaluatedwithablood-gasanalyzer.ExperimentswereperformedusingRMVECstreatedwithandwithouttransferAd-15-LOX-1orAd-vectorbothunderhypoxiaandnormoxiaconditionat12,24,48,72hours.Theefficacyofthegenetransferwasassessedbyimmunofluorescencestaining.CellsproliferationwasevaluatedbytheCCK-8method.RNAandproteinexpressionsof15-LOX-1,VEGF-A,VEGFR-2,eNOsandPPAR-rwereanalyzedbyreal-timereversetranscriptionpolymerasechainreaction(RT-PCR)andWesternblot.RESULTS:RoutineevaluationforFITC-markedCD31showedthatcellswerepure.Theresultsofblood-gasanalysisshowedthatwhenthecultureswereexposedtohypoxiaformorethan2hours,thePo2was4.5to5.4Kpa.WeverifiedRMVECscouldbeinfectedwithAd-15-LOX-1orAd-vectorviaFluorescencemicroscopy.CCK-8analysisrevealedthattheproliferativecapacitiesofRMVECsinhypoxicgroupweresignificantlyhigherateachtimepointthantheywereinnormoxicgroup(P<0.05).Inahypoxiccondition,theproliferativecapacitiesofRMVECsin15-LOX-1groupweresignificantlyinhibited(P<0.05).Real-timeRT-PCRanalysisrevealedthattheexpressionsofVEGF-A,VEGF-R2andeNOsmRNAincreasedinhypoxiagroupcomparedwithnormoxiagroup(P<0.01).However,theexpressionsof15-LOX-1,PPAR-rmRNAdecreasedinhypoxiagroupcomparedwithnormoxiagroup(P<0.01).Italsoshowedthatinahypoxiccondition,theexpressionsofVEGF-A,VEGF-R2andeNOsmRNAdecreasedsignificantlyin15-LOX-1groupcomparedwithhypoxiagroup(P<0.01).However,15-LOX-1andPPAR-rmRNAincreasedsigni