简介:【摘要】目的:本研究观察不同类型非去极化肌松药对米库氯铵药效学的影响,探讨适合声带息肉摘除术等短小手术中的肌松药物的联合作用。方法:选择120 例美国麻醉师协会( ASA) Ⅰ ~ Ⅱ级耳鼻喉科患者择期行声带息肉摘除手术 ,使患者或者亲属签署麻醉知情同意书,随机分为 4 组( C、V、R、M组):C组为空白对照组,预注 5ml 生理盐水;V组为预注维库溴铵组,预注量为0.0086mg/kg;R组为预注罗库溴铵组,预注量为0.061mg/kg;M组为预注米库氯铵组,预注量为0.0134mg/kg,3min后给予插管剂量米库氯铵 0.1206mg/kg。结果:对四组各个时间点的SBP、DBP和HR进行比较,四组患者的肌松效果和恢复情况显示与C组相比较,V组、R组和M组的插管时间和起效时间均缩短;与R组相比,V组和M组的插管时间、起效时间均缩短;与V组相比,M组的插管时间和起效时间均缩短。差异均具有统计意义(P<0.05)。与C组相比,V组、R组和M组的无反应期和临床作用时间延长(
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简介:ToobservetheeffectofGardeniaextractZGontheadsorptionquantityofherpessimplexvirustype1(HSV-1)soastoexplorethemechanismofitsantiviralactivity,fluoresceinisothiocyanate(FITC)wasusedasthefluorescentprobetolabelvirusesandheparinsodiumwasusedascontrol.Meanwhile,theeffectofGardeniaextractZGontheadsorptionquantityonthesurfaceofHep-2cellswasdeterminedbyflowcytometry.ItwasdemonstratedthatadsorptionofHSV-1onthesurfaceofHep-2cellsexhibitedthecharacterofsaturationandspecificityandheparinsodiumcouldpreventattachmentofvirusesonthesecells.Theseresultsareinaccordwiththosereportedpreviously.Itwasalsoprovedthatthemannerofdrug-usepriortoadsorptionorsimultaneoususeofdrugandadsorptionwasbetterthanadsorptionpriortodrug-use,andtheinhibitionratesoftheformerandlattermannerwere84.76%and82.92%respectively.Threemannersofdrug-usewithGardeniaextractZGwerealleffectivetoreducetheadsorptionquantityofviruses,especiallythemannerofsimultaneoususeofdrugandadsorptionwithanadsorptioninhibitionrateof68.46%.Fromtheaboveobservation,itisapparentthatthemechanismofanti-viralactivityofGardeniaextractZGmaybeviaseveralstepsinvolvedintheHSV-1adsorption.
简介:WeareracingwithHIV-1,theetiologicagentforAIDSinhumanbeings[1,2],withtwopossibleendconsequences:ifwewin,HIV-1willbeunderourcontrolbyimmunologicortherapeuticmeasures;ifHIV-1wins,theSIVAfricanmonkeys'storywouldrepeatinhumans,i.e.,onlythefewindividualsthatarenotkilledbythevirus
简介:Wehavepreviouslydemonstratedtheabilityofmalariaparasitestointerferewithspecificimmuneresponses.CD4Tcellsspecifictoparasiteantigens,butnotCD4Tcellsspecifictoanirrelevantantigen,ovalbumin(OVA),aredeletedviaapoptosisduringmalariainfection.Itisofinterest,therefore,toinvestigatetheimmuneresponsesthatdevelopedfollowingvaccinationwiththe19kDacarboxylterminusofthemerozoitesurfaceprotein1(MSP119)inmicethathadpreviouslyexperiencedmalariainfection.Inthisstudy,pre-exposureofmicetoPlasmodiumyoeliielicitednativeanti-MSP119antibodyresponses,whichcouldbeboostedbyvaccinationwithrecombinantMSP119,Likewise,infectionofMSP119-primedmicewithPlasmodiumyoelii(P.yoelii)ledtoanincreaseofanti-MSP119antibodies.MSP119vaccinationofmalariapreexposedmiceorimmunizationbyinfection/cureofMSP119-primedmiceenabledthemicetosurvivechallengeinfection,withtheformergrouphavingslightlylowerparasitaemia.Thedatasuggestthatexposuretomalariainfectionprimesanaturalimmuneresponsewhichcanbeboostedbyvaccination.Thisinformationisrelevanttothedevelopmentofavaccineforuseinindividualslivinginmalaria-endemicareas.
简介:摘要:目的 探讨运用了自创3M编带的固定法在改善胸外科管道护理安全管理中的实际应用效果。方法:选取广州市番禺区中心医院心胸外科2020年7月-2021年6月收治的100例患者作为研究对象,按照就诊时间分为对照组(n=48)和研究组(n=52),对照组为2020年7月-12月间使用弹力胶布高举平台法固定管道的患者,研究组为2021年1月-6月间使用自创3M编带固定法固定管道的患者,对比两组患者管道不良事件发生率以及患者护理满意度。2021年1月起,针对胸外科患者管道护理的安全性,统一运用了自创3M编带的固定法固定管道。结果:研究组管道不良事件发生率5.77%较对照组(20.83%)更低,差异有统计学意义(P
简介:ToexplorethemechanismoftheinhibitionofHIV-1byMycoplasmafermerttans,culturesupernatantsandthallodicproteinsfromM.fermerttansPG18werepreparedandtheproteincomponentsofthesupernatantswerepurifiedwithhighperformanceliquidchromatography(HPLC).Theinhibitoryactivitiestoreversetranscriptase(RT)andthenucleaseactivitiesweredetected;theinfluenceofM.fermerttansonIL-10secretionbybothnormalandH1V-1infectedhumanPBMCweredetermined,andtheinhibitoryeffectofrhIL-10onH1V-1replicationwasdetectedwithEI,ISAmethod.Theresultsshowedthatthepurifiedproteinswithamolecularweightof67-100kDaor10-25kDashoweda36%or34%inhibitoryac-tivitytoRTandpartialnucleaseactivity.ThethallodicproteincouldinducebothnormalandH1V-1infectedPBMCtosecretIL-10remarkably,andtothelatter,thiseffectwasmoreapparent.WhilerhIL-10couldinhibitreplicationofH1V-1inPB-MCinvitroinadose-dependantmanner.ItconcludesthattheinhibitoryeffectoftheM.fermentansPG18culturesupernatantsonRTandthepromotingeffectofPG18thallodicproteinonIL-10secretioninPBMCexplainthemechanismsofinhibitiontoHIV-1byM.fermentansPG18.
简介:摘要:目的 通过建立骨关节炎模型探讨整合素pI和GIT 1对软骨细胞的影响,及二者之间的调控关系。对临床上骨性关节炎的治疗起指导作用。 方法 采用约一周龄的年乳大鼠20只,用定量PCR和Western Blotting检测对照组、pcDNA3.1空载体转染组、pcDNA3.1-GIT 1过表达组、pcDNA3 . I -integrin-p1过表达组、integrin-p 1 siRNA组和NC siRNA组中,integrin-p1和GIT1的mRNA和蛋白质的表达水平,来判断integrin-p1和GIT1之间的调控关系。结论 integrin-p1可以调控GIT1的表达,GTI1可能是位于integrin-pI下游的一个关键分子。
简介:ToinvestigatethephenotypicknockoutofHIV-1chemokinecoreceptorCXCR4andCCR5byintrakinesanditsinhibitoryeffectonHIV-1infection.PrimaryhumanPBLsweretransducedwiththerecombinantvectorpLNCX-R-K-S-K(△NGFR),followedbyanti-NGFR/anti-IgG-magneticbeadmethodselectionandFCMdetection.ThetransducedPBLswereinfectedwithDP1HIV-1virusthereafterenvelope-mediatedsyncytiumformationandp24detectionwerecarriedouttostudytheblockageofHIV-1infectionbyco-inactivationofCCR5andCXCR4.pLNCX-R-K-S-K(△NGFR)-transducedPBILswereisolatedwithananti-NGFR/anti-IgG-magneticbeadmethod.Afterisolation,about70%ofthePBLswerepositivefortheNGFRmarker.WhenthetransducedPBLswereinfectedwithDP1HIV-1virus,envelop-mediatedsyncytiumformationwasalmostcompletelyinhibitedbypLNCX-R-K-S-K(△NGFR)transfection.Also,p24antigenwasverylowintheculturesofpLNCX-R-K-S-K(△NGFR)transducedPBLs.pLNCX-R-K-S-K(△NGFR)transductioninhibitedtheproductionofDP1p24antigenby15%,43%and19%ondays4,7and10respectively.ThelymphocyteswiththephenotypicknockoutofCCR5andCXCR4couldprotectprimaryhumanPBLsfromDP1HIV-1virusinfection.
简介:TodeterminewhetherthepossessionofcertainHLA-DQA1alleleswasassociatedwiththeriskofdevelopingidiopathicdilatedcardiomyopathy(IDC)andtosubstantiatetheroleofanautoimmunologicpathogenesisinIDC.TypetheallelesofHLA-DQA1bypolymerasechainreactionwithsequence-specificprimers(PCR-SSP)techniquein38patientsofidiopathicdilatedcardiomyopathy(7womenand31men),agedfrom17to56yearsoldwithdiagnosisbeingaccordingtoWorldHealthOrganizationcriteria(IDCgroup),in50patientsofend-stageheartfailureofknownetiology(18womenand32men),withagesrangingfrom34to72(HFgroup),andinthecontrolgroupconsistingofpresumably100healthysubjects(39womenand61men)fromthehealthsurvey,agedfrom30to59yearsold.ThefrequencyofHLA-DQA1*0501intheDCMpatientswassignificantlyelevatedthanthatintheHFandthecontrolgroup.MolecularanalysisoftheDQA1genepolymorphismperformedinthethreesubgroupsshowsanincreasedfrequencyofDQA1*0501amongpatientswithlessEF.TheHFgroupcarriesahighfrequencyofHLA-DQA1*0301.AnincreasedfrequencyofDQA1*0201andDQA1*0103wasfoundinthecontrolgroup.HLA-DQA1*0501isanassociatedgeneofidiopathicdilatedcardiomyopathyandthepossessionofDQA1*0301maybeindicativeoftheknownetiologicheartfailure,suggestingthatthemechanismsinvolvedinthepathogenesisofIDCandotherwiseheartfailurearedifferent.ImmunologicabnormalitiesmaybeamajorcontributortothesusceptibilityofdevelopingofIDC.
简介:TofindouttheprotectivepolypeptideepitopesofHCVHVR1,theantigenieityofthesyntheticpepfidewaspredictedbycomputermodelingandverifiedbyELISAandlymphocytetransformationtest.Itwasfoundthattheoutcomeofthecomputermodelingwasinaccordwiththeexperimentalresults.Themethodbyusingcomputermodelingwouldbeaeconomicapproachbywhichtheprotectivepeptidescouldbeidentifiedquickly.
简介:摘要 目的 通过随机对照试验,客观评价院内自拟方肛肠坐浴1号方治疗痔疮术后的临床疗效,并考察其安全性。方法 400个病例通过随机分组的方法将病例随机分为试验组和对照组,试验组和对照组各200例,试验组术后用肛肠坐浴1号方坐浴熏洗2周,对照组高锰酸钾坐浴熏洗2周,观察分析术后伤口疼痛水肿情况。结果 试验组有效率为100%,明显高于对照组的87.3%(P<0.05)。结论 肛肠坐浴1号方能有效缓解痔疮术后患者的肛缘水肿、疼痛,减少出血,减少并发症发生,突显出中医外治法的优势与特色,值得临床进一步推广。