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109 个结果
  • 简介:【 摘要 】 目的 :分析医院集中招标采购医用耗材的实践状况及优化对策。 方法 :归纳、整理相关文献,探讨医用耗材集中招标采购的实践情况,基于此提出相应的优化对策并于我院进行试验,对比试验前后 1 年内 4 个季度招标采购中医用耗材费用占比。 结果 :我院于 2019 年 1 月开始试用医用耗材集中招标采购优化对策,试验后,招标采购中医用耗材费用占比逐渐降低,而第 3 季度、第 4 季度招标采购中医用耗材费用均低于试验前,数据差异有统计学意义 ( P < 0.05 ) 。 结论 : 招标评审问题、采购问题是阻碍医用耗材集中招标采购效率的关键因素,为此,就需要建立供应商评价体系,构建信息化审查系统,才能 推动医用耗材集中招标采购制度的健康发展,提高医院的服务质量。

  • 标签: 医用耗材 集中招标采购 实践 优化对策
  • 简介:摘要:医院医疗作为社会发展过程中不可或缺的重要组成部分,医疗设备更是影响我国医院、医疗发展的重要因素,在我国多年医疗设备采购发展的背景下,医疗设备招标采购出现在人们面前,并逐步成为医院运行中备受人们关注的焦点问题。由此,本文拟从当下医院医疗设备招标采购中的典型问题入手进行阐述、分析,从中思考应对医院医疗设备招标采购中典型问题的对策,希望能对后续医院工作提供帮助。

  • 标签: 医院 医疗设备 招标采购 问题 对策
  • 简介:ToobservetheeffectofGardeniaextractZGontheadsorptionquantityofherpessimplexvirustype1(HSV-1)soastoexplorethemechanismofitsantiviralactivity,fluoresceinisothiocyanate(FITC)wasusedasthefluorescentprobetolabelvirusesandheparinsodiumwasusedascontrol.Meanwhile,theeffectofGardeniaextractZGontheadsorptionquantityonthesurfaceofHep-2cellswasdeterminedbyflowcytometry.ItwasdemonstratedthatadsorptionofHSV-1onthesurfaceofHep-2cellsexhibitedthecharacterofsaturationandspecificityandheparinsodiumcouldpreventattachmentofvirusesonthesecells.Theseresultsareinaccordwiththosereportedpreviously.Itwasalsoprovedthatthemannerofdrug-usepriortoadsorptionorsimultaneoususeofdrugandadsorptionwasbetterthanadsorptionpriortodrug-use,andtheinhibitionratesoftheformerandlattermannerwere84.76%and82.92%respectively.Threemannersofdrug-usewithGardeniaextractZGwerealleffectivetoreducetheadsorptionquantityofviruses,especiallythemannerofsimultaneoususeofdrugandadsorptionwithanadsorptioninhibitionrateof68.46%.Fromtheaboveobservation,itisapparentthatthemechanismofanti-viralactivityofGardeniaextractZGmaybeviaseveralstepsinvolvedintheHSV-1adsorption.

  • 标签: 栀子提取物ZG 单纯疱疹病毒1型 吸附量 流动细胞计数
  • 简介:WeareracingwithHIV-1,theetiologicagentforAIDSinhumanbeings[1,2],withtwopossibleendconsequences:ifwewin,HIV-1willbeunderourcontrolbyimmunologicortherapeuticmeasures;ifHIV-1wins,theSIVAfricanmonkeys'storywouldrepeatinhumans,i.e.,onlythefewindividualsthatarenotkilledbythevirus

  • 标签: HIV-1 AIDS 治疗作用 传染病
  • 简介:Wehavepreviouslydemonstratedtheabilityofmalariaparasitestointerferewithspecificimmuneresponses.CD4Tcellsspecifictoparasiteantigens,butnotCD4Tcellsspecifictoanirrelevantantigen,ovalbumin(OVA),aredeletedviaapoptosisduringmalariainfection.Itisofinterest,therefore,toinvestigatetheimmuneresponsesthatdevelopedfollowingvaccinationwiththe19kDacarboxylterminusofthemerozoitesurfaceprotein1(MSP119)inmicethathadpreviouslyexperiencedmalariainfection.Inthisstudy,pre-exposureofmicetoPlasmodiumyoeliielicitednativeanti-MSP119antibodyresponses,whichcouldbeboostedbyvaccinationwithrecombinantMSP119,Likewise,infectionofMSP119-primedmicewithPlasmodiumyoelii(P.yoelii)ledtoanincreaseofanti-MSP119antibodies.MSP119vaccinationofmalariapreexposedmiceorimmunizationbyinfection/cureofMSP119-primedmiceenabledthemicetosurvivechallengeinfection,withtheformergrouphavingslightlylowerparasitaemia.Thedatasuggestthatexposuretomalariainfectionprimesanaturalimmuneresponsewhichcanbeboostedbyvaccination.Thisinformationisrelevanttothedevelopmentofavaccineforuseinindividualslivinginmalaria-endemicareas.

  • 标签: 疟疾 寄生虫感染 特异性免疫反应 CD4 T细胞 疫苗接种
  • 简介:ToexplorethemechanismoftheinhibitionofHIV-1byMycoplasmafermerttans,culturesupernatantsandthallodicproteinsfromM.fermerttansPG18werepreparedandtheproteincomponentsofthesupernatantswerepurifiedwithhighperformanceliquidchromatography(HPLC).Theinhibitoryactivitiestoreversetranscriptase(RT)andthenucleaseactivitiesweredetected;theinfluenceofM.fermerttansonIL-10secretionbybothnormalandH1V-1infectedhumanPBMCweredetermined,andtheinhibitoryeffectofrhIL-10onH1V-1replicationwasdetectedwithEI,ISAmethod.Theresultsshowedthatthepurifiedproteinswithamolecularweightof67-100kDaor10-25kDashoweda36%or34%inhibitoryac-tivitytoRTandpartialnucleaseactivity.ThethallodicproteincouldinducebothnormalandH1V-1infectedPBMCtosecretIL-10remarkably,andtothelatter,thiseffectwasmoreapparent.WhilerhIL-10couldinhibitreplicationofH1V-1inPB-MCinvitroinadose-dependantmanner.ItconcludesthattheinhibitoryeffectoftheM.fermentansPG18culturesupernatantsonRTandthepromotingeffectofPG18thallodicproteinonIL-10secretioninPBMCexplainthemechanismsofinhibitiontoHIV-1byM.fermentansPG18.

  • 标签: 抑制作用 HIV-1 支原体发酵 IL-10 HPLC 分泌物
  • 简介:摘要:目的 通过建立骨关节炎模型探讨整合素pI和GIT 1对软骨细胞的影响,及二者之间的调控关系。对临床上骨性关节炎的治疗起指导作用。 方法 采用约一周龄的年乳大鼠20只,用定量PCR和Western Blotting检测对照组、pcDNA3.1空载体转染组、pcDNA3.1-GIT 1过表达组、pcDNA3 . I -integrin-p1过表达组、integrin-p 1 siRNA组和NC siRNA组中,integrin-p1和GIT1的mRNA和蛋白质的表达水平,来判断integrin-p1和GIT1之间的调控关系。结论 integrin-p1可以调控GIT1的表达,GTI1可能是位于integrin-pI下游的一个关键分子。

  • 标签: 骨关节炎 关节软骨细胞 整合素阳 GITI 细胞增殖与凋亡
  • 简介:ToinvestigatethephenotypicknockoutofHIV-1chemokinecoreceptorCXCR4andCCR5byintrakinesanditsinhibitoryeffectonHIV-1infection.PrimaryhumanPBLsweretransducedwiththerecombinantvectorpLNCX-R-K-S-K(△NGFR),followedbyanti-NGFR/anti-IgG-magneticbeadmethodselectionandFCMdetection.ThetransducedPBLswereinfectedwithDP1HIV-1virusthereafterenvelope-mediatedsyncytiumformationandp24detectionwerecarriedouttostudytheblockageofHIV-1infectionbyco-inactivationofCCR5andCXCR4.pLNCX-R-K-S-K(△NGFR)-transducedPBILswereisolatedwithananti-NGFR/anti-IgG-magneticbeadmethod.Afterisolation,about70%ofthePBLswerepositivefortheNGFRmarker.WhenthetransducedPBLswereinfectedwithDP1HIV-1virus,envelop-mediatedsyncytiumformationwasalmostcompletelyinhibitedbypLNCX-R-K-S-K(△NGFR)transfection.Also,p24antigenwasverylowintheculturesofpLNCX-R-K-S-K(△NGFR)transducedPBLs.pLNCX-R-K-S-K(△NGFR)transductioninhibitedtheproductionofDP1p24antigenby15%,43%and19%ondays4,7and10respectively.ThelymphocyteswiththephenotypicknockoutofCCR5andCXCR4couldprotectprimaryhumanPBLsfromDP1HIV-1virusinfection.

  • 标签: 爱滋病病毒 基因类型 HIV-1 CXCR4 CCR5 基因表达
  • 简介:TodeterminewhetherthepossessionofcertainHLA-DQA1alleleswasassociatedwiththeriskofdevelopingidiopathicdilatedcardiomyopathy(IDC)andtosubstantiatetheroleofanautoimmunologicpathogenesisinIDC.TypetheallelesofHLA-DQA1bypolymerasechainreactionwithsequence-specificprimers(PCR-SSP)techniquein38patientsofidiopathicdilatedcardiomyopathy(7womenand31men),agedfrom17to56yearsoldwithdiagnosisbeingaccordingtoWorldHealthOrganizationcriteria(IDCgroup),in50patientsofend-stageheartfailureofknownetiology(18womenand32men),withagesrangingfrom34to72(HFgroup),andinthecontrolgroupconsistingofpresumably100healthysubjects(39womenand61men)fromthehealthsurvey,agedfrom30to59yearsold.ThefrequencyofHLA-DQA1*0501intheDCMpatientswassignificantlyelevatedthanthatintheHFandthecontrolgroup.MolecularanalysisoftheDQA1genepolymorphismperformedinthethreesubgroupsshowsanincreasedfrequencyofDQA1*0501amongpatientswithlessEF.TheHFgroupcarriesahighfrequencyofHLA-DQA1*0301.AnincreasedfrequencyofDQA1*0201andDQA1*0103wasfoundinthecontrolgroup.HLA-DQA1*0501isanassociatedgeneofidiopathicdilatedcardiomyopathyandthepossessionofDQA1*0301maybeindicativeoftheknownetiologicheartfailure,suggestingthatthemechanismsinvolvedinthepathogenesisofIDCandotherwiseheartfailurearedifferent.ImmunologicabnormalitiesmaybeamajorcontributortothesusceptibilityofdevelopingofIDC.

  • 标签: HLA-DQA1 等位基因 基因多态性 扩张型心肌病 免疫学
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  • 简介:TofindouttheprotectivepolypeptideepitopesofHCVHVR1,theantigenieityofthesyntheticpepfidewaspredictedbycomputermodelingandverifiedbyELISAandlymphocytetransformationtest.Itwasfoundthattheoutcomeofthecomputermodelingwasinaccordwiththeexperimentalresults.Themethodbyusingcomputermodelingwouldbeaeconomicapproachbywhichtheprotectivepeptidescouldbeidentifiedquickly.

  • 标签: 抗原性 HCV HVR1 缩氨酸 计算机模型 淋巴细胞
  • 简介:摘要 目的 通过随机对照试验,客观评价院内自拟方肛肠坐浴1号方治疗痔疮术后的临床疗效,并考察其安全性。方法 400个病例通过随机分组的方法将病例随机分为试验组和对照组,试验组和对照组各200例,试验组术后用肛肠坐浴1号方坐浴熏洗2周,对照组高锰酸钾坐浴熏洗2周,观察分析术后伤口疼痛水肿情况。结果 试验组有效率为100%,明显高于对照组的87.3%(P<0.05)。结论 肛肠坐浴1号方能有效缓解痔疮术后患者的肛缘水肿、疼痛,减少出血,减少并发症发生,突显出中医外治法的优势与特色,值得临床进一步推广。

  • 标签: 痔疮 混合痔 肛肠坐浴1号方 疼痛
  • 简介:摘要:甲真菌病是指由皮肤癣菌、酵母菌和非皮肤癣菌性霉菌(简称其他霉菌)侵犯甲板和/或甲床所致的疾病。甲真菌病的发病与年龄、性别及部位均有关。男性发病率高于女性,趾甲发病多于指甲,足拇趾甲罹患率高于其他甲。本文将1例以脑出血后偏瘫侧严重甲真菌感染患者的资料进行了整理、分析,并复习了相关文献。。

  • 标签: 脑出血,意识障碍 肺部感染 机械通气 偏瘫侧甲真菌感染
  • 简介:【摘要】爆发性心肌炎起病急骤、病程凶险,患者可于数日内出现急性心力衰竭、心源性休克、严重心律失常甚至猝死,预后极差。当药物治疗无效时,通常需给予积极的机械循环辅助,体外膜肺氧和(ECMO)、主动脉内球襄反搏(IABP)为主要的辅助方法。横纹肌溶解综合征是指由于横纹肌损伤引起细胞的溶解,释放大量肌红蛋白、肌酸磷酸肌酶、乳酸脱氢酶进入外周血液造成的临床综合征,爆发性心肌炎合并恶性心率失常时,患者病情已处于严重阶段,如再合并横纹肌溶解,则病情更为复杂,临床护理难度大。

  • 标签: 爆发性心肌炎 横纹肌溶解 护理干预