NucleosideisthemainclassofactivecomponentsinCordycepssinensis.Thin-layerchromatography(TLC)isoneofthemostcommonlyusedmethodsinpharmacopoeiasforanalyzingchemicalcomponentsofherbalmedicine.SincetheisocraticelutionmethodcannotbeappliedsuccessfullyinTLCanalysisforseparatingallthenucleosidecomponents,thestepwisegradientelutionhasbeendevelopedinthisworktoseparateeightnucleosidestandardswithsuccess.Inthisway,quantitativeanalysesofthesamplesofCordycepssinensiswereachievedviatheproposedTLCprocedurecoupledwiththescanningdensitometrictechniquesofCAMAGandTLCQAmethodsforqualitativeandquantitativeanalysis.