学科分类
/ 25
500 个结果
  • 简介:Inthepastdecade,usesofantisepticsanddisinfectantsinhospitalsandotherhealthcarecentersarerathercommon,butthechancetodevelopresistancetoantisepticsanddisinfectantsisalsoincreased.Acinetobacterbaumanniiisoneoftheopportunisticbacteriainvolvinginthenosocomialinfection.Inthepresentstudy,thecorrelationoftheantisepticresistanceinA.baumanniiandtheantisepticresistancegeneqacEΔ1wasinvestigatedbymeansofdeterminationofMICs.Meanwhile,theMICsofglutaraldehyde,chlorhexidine,benzalkoniumbromide,iodophorandtrichloroisocyanurateto80clinicalisolatesofA.baumanniiweredetectedbytubedilutionassayandtheresistancegenesintI1andqacEΔ1intheseisolateswereamplifiedbyPCRandverifiedbyDNAsequencer.ItwasfoundthattheMIC50forthese5antisepticstestedwere32,8,8,4and1μg/mlrespectively,andthedetectionratesofintI1andqacEΔ1genewere60.0%and77.6%respectively.Inaddition,55%ofthe80isolatessimultaneouslypossessedbothintllandqacEΔ1gene,andthepercentageofantisepticresistanceofA.baumanniicarringbothgenestobenzalkoniumbromidewerehigherthanthatwithoutthesetwogenes,however,therewasnosignificantdifferencebetweenintllandqacEΔ1gene.Theresultinbactericidalefficiencyassayindicatedthatchlorhexidinecouldstillproducerapidandstrongbactericidaleffectatconcentrationof1MICafter10minexposure.TheseresultssuggestthattheantisepticresistanceofA.baumanniitovariousantisepticsiscorrelatedwiththepresenceoftheantisepticresistancegenesqacEΔ1inbacteria,thuswarningthattheincreaseoftheantisepticresistanceshouldnotbeignoredandtherelativehighconcentrationorprolongedapplicationtimeisrequiredtoachieveasufficientbactericidaleffect.

  • 标签: 鲍氏不动杆菌 消毒剂抗药性 耐药基因qacEΔ1 整合子 相关性
  • 简介:【摘要】:目的:研析在急诊内科的治疗中,给与不同的药物治疗对老年重症心力衰竭患者的临床参考价值。方法:选取老年重症心力衰竭患者80,均为我院收治,随机原则均分为A组及B组,对A组患者行常规治疗,对B组行美托洛尔联用厄贝沙坦氢氯噻嗪治疗,对两组患者的临床治疗治疗前后的心功能指标进行分析。结果:治疗前两组患者心功能并无较大差异(P>0.05);治疗后,B组患者的心功能改善效果较A组更为显著(P<0.05)。结论:对于急诊内科的老年重症心力衰竭患者采用美托洛尔联用厄贝沙坦氢氯噻嗪治疗,患者症状显著缓解,疗效可观。

  • 标签: 老年重症心力衰竭 美托洛尔 厄贝沙坦氢氯噻嗪
  • 简介:InordertorevealvariationandrevolutionofNPgenesofhumanavianH_5N_1influenzavirusstrains,theNPgeneofahumanavianH_5N_1influenzavirusstraininGuangdongwassequencedandtheglobalNPgenesofstrainswereretrieved.ThesequenceswereanalyzedbyDNAStar5.0,andtheevolu-tionaryspeedwasstudiedwithreferencetotheepidemiologicaldata.ItwasfoundthatNPgenesof45strainsduring1997-2006werehomologicallyclassifiedintothreegroups:strainsin1997-1998,strainsin2004-2005andstrainsfrom2003to2006.Therewere35substitutionsinNPsinallstrainsaccountingforaratioof7.03%(35/498).Anadditionalglycoproteindomain(NGT_(430-432))wasfoundinNPgenesinthestrainsof2003-2006,themutationofN_(370)SinGD-01-06resultedinoccurrenceofonemoreglyco-proteindomain(NES_(368-370)).Inthesynonymousvariation,K_svaluesinNPwere2.03×10~(-5)-2.55×10~(-5)Nt/dandK_avaluesinNPwere1.58×10(-6)-3.10×10~(-6)Nt/d.Theredidn′texistobviouslyselectivepres-sure.Anadditionalglycoproteindomainineverystrainof2003-2006andonemoreinstrainGD-01-06mightchangetheantigenicityofhumanavianH_6N_1influenzavirus.ThevariationonhumanavianH_5N_1influenzastrainsoccurredfrequentlyinthenaturalworld,whichwouldresultinhighprobabilityofhu-man-humantransmissionalongwiththenaturalevolutionofthevirus.

  • 标签: NP基因 禽流感 H5N1病毒 基因表达
  • 简介:Wereportedanovelmammalianreovirus,designedBYD1,isolatedfromthroatswabsofpatientswithsevereacuterespiratorysyndrome(SARS),in2003.Inthepresentstudy,wefirstlycomparedthegenomeelectrophoreticmigrationpatternsofreovirusBYD1with3prototypereovirusstrainsbypolyacrylamidegelelectrophoresis(PAGE)anddeterminedthecompletenucleotidesequenceoftheS1genesegmentofBYD1bysingleprimeramplificationtechnique.TheelectropherogramofBYD1wasdifferentfromthoseofthe3prototypestrainsandanyotherreovirusisolatesreportedbefore.TheentireS1segmentsequenceofBYD1is1437bplongwithtwomeaningfulopenreadingframes(ORFs).ThelongestORFencodesσ1,thecellattachmentprotein,andthesecondlongestORFsupposedlyencodesσ1s,animportantnonstructuralvirulencefactor.TheterminalsequencesofS1segmentare5'GCUAand3'UCAUC,whichareconsistentwiththoseofothermammalianreoviruses.Thehighesthomologyofdeducedσ1aminoacidsequenceis64%identitywithknownmammalianreoviruses.PhylogeneticanalysisofbothS1nucleotidesequenceandσ1aminoacidsequenceindicatedtheBYD1isolatebelongedtoanewcladeofserotype2group.TheresultsofthisstudyshowedthattheBYD1S1segmentwasmarkedlydifferentfromthoseofisolatesreportedbeforeandBYD1wasanovelhumanreovirusisolate.

  • 标签: SARS病人 呼肠孤病毒 分离 分子特征 S1片段
  • 简介:

  • 标签:
  • 简介:

  • 标签:
  • 简介:摘要:目的:观察PDCA循环应用于口腔门诊护理管理的临床效果。方法:随机分组,对照组使用常规护理管理方法,观察组使用PCDA循环护理管理方法。结果:观察组对医院环境评分为(92.36±1.58)分、医疗器械评分为(94.15±1.69)分、安全意识评分为(97.00±2.33)分、护理态度评分为(90.41±2.30)分,观察组满意度为97.7%(42/43),与对照组差异显著(P<0.05)。结论:将PCDA循环应用于口腔门诊护理中,具有明显的效果,可以为患者提供良好的服务环境,使患者在就诊中感到舒适,利于医院可持续发展,应在护理过程中广泛推广。

  • 标签: PDCA循环 口腔门诊 护理管理
  • 简介:

  • 标签:
  • 简介:

  • 标签:
  • 简介:ToconstructanexpressionvectorcontainingtheE1glycoproteingeneofrubellavirusforthestudyontheeffectofmutationoftheE1geneglycoproteinandtheanalysisofphylogeneticdifferencesofsequences,thegeneencodingtheE1envelopeglycoproteinwasamplifiedfromrubellavirus,JinanstrainJR23,byRT-PCRandligatedintoPMD-18Tvector.TheclonesthatcarriedtheE1genewereidentifiedafteramprselectionandanalysisofrestrictionenzymedigestion.AftersequencingthisgenewasanalyzedbyDanstarandWinstarprograms,andthemapofphylogenetictreewasdrawn.ThecloneofE1glycoproteinwasthusconstructed.ItwasfoundthatthesequencedifferencesbetweenJR23strainandtheTCRBstrainfromJapanandthosebetweenJR23strainandThomasstrainofEnglandwererathersmallwithdifferencevaluesof0.9%and1.2%respectively.YetthosebetweenJR23strainandBRD2strainfromBeijingandthosebetweenJR23strainandXG379strainfromHongKongwerecomparativelylargerwithdifferencevaluesof7.6%and7.3%respectively.ThesequenceofJR23strainwithotherstrainswaslessthan3%excepttheNCstrain(3.7%).ItconcludesthattheconstructionofE1glycoproteingeneoffersanapproachtostudytherelationshipbetweenstructuresandfunctionsofE1geneanditsgeneproducts.Inthephylogenetictree,itshowsthattherearesignificantdifferencesinthesequencesofrubellavirusisolatedinChina,andthismightbehelpfultodevelopaneffectivesubunitvaccine.

  • 标签: 无性繁殖 序列分析 糖蛋白包膜 E1基因 风疹病毒 JR23株
  • 简介:Theimmuneefficiencyofarecombinantadenovirustype5withtype35fibercontainingHIV-1gaggene(rAd5/F35-mod.gag)wasinvestigatedinBALB/cmice,inwhichtherAd5/F35-mod.gagwasfirstlyidentifiedwithPCR,thentransfectedto293cellsandtheinvitroexpressionlevelofGagproteinwasdeterminedbyWesternblottingandindirectimmuno-fluorescentassay.MicewereimmunizedwithintramuscularinjectionsofrAd5/F35-mod.gag,rAd5-mod.gagorDNAandwereboostedafter3weeks.Totesttheeffectofpre-existinganti-viralimmunityonimmunization,micewerealsoinjectedwithAd5-GFPvectorandthenimmunized4and7weekslaterwithAd5/F35-mod.gagvector.TheP24-specificIgGantibodyinseraofimmunizedmicewasdeterminedbyELISAandthespecificcytotoxicTlymphocyte(CTL)responsewasassayedbyintracellularcytokinestaining.ItwasdemonstratedthattherAd5/F35-mod.gagvectorcouldexpressefficientlytheHIVGagproteinin293cellsinvitroandinducestrongHIV-specificimmuneresponsesinvivo.ThestrongestCTLandserumIgGresponseoccurredwhenmicewereimmunizedtwicewithinjectionofrAd5/F35alone,buttheanti-Ad5antibodyafterprimaryinfectionwithadenoviruscouldinhibitthespecificimmuneresponsesinducedbyrAd5/F35vector.ItisconcludedthatsingleimmunizationwithrecombinantadenovirusrAd5/F35-mod.gagcaninducespecificCTLandserumIgGantibodyresponsesinmice,buttheimmunogenicityofrAd5/F35iscomparablyweakerthanthatofrAd5.

  • 标签: 重组腺病毒5型载体 35型纤突 HIV-1 GAG基因 爱滋病疫苗 免疫原性
  • 简介:【摘要】目的:探究预警式护理对于老年冠心病心律失常患者的护理效果。方法:以科室内2019年1月-2020年1月期间收治的老年冠心病心律失常患者为对象,遴选出60进行研究。利用随机数字表法分组,其中30组成对照组,实施常规护理措施;另30组成观察组,实施预警式护理。结果:观察组患者心律失常控制有效率高达93.33%,患者满意度为90.00%,两项数据均高于对照组,检验值(P<0.05)。结论:预警式护理能够有效控制老年冠心病患者心律失常的发生,且患者满意度较高,具有实施价值。

  • 标签: 老年冠心病 心律失常 预警式护理
  • 简介:【摘要】目的:研究探讨急诊治疗重症哮喘合并呼吸衰竭患者的临床疗效。方法:从2019年3月-2020年3月于我院进行治疗的患者中随机选取60作为研究对象,按患者的意愿将其分为研究组及对照组,对照组采取常规治疗,研究组在其基础上加无创机械通气治疗,对比观察两组治疗前后的通气效果及ACT评分。结果:治疗前,患者组间ACT评分差异不大(P>0.05);治疗后,研究组患者ACT评分及通气效果显著优于对照组患者(P<0.05)。结论:对重症哮喘合并呼吸衰竭患者采取常规治疗联合无创机械通气治疗可以显著减轻患者病死率,临床应用价值可观。

  • 标签: 重症哮喘 呼吸衰竭 急诊治疗
  • 简介:目的:基于重组流感病毒载体的呼吸道合胞病毒(RSV)疫苗的构建及在小鼠体内的免疫保护效果评价。方法:构建并拯救表达RSV A2型G蛋白胞外结构域(Gecto)的重组甲型流感病毒,将其命名为PR8NAGecto/WSN。体外验证重组病毒G蛋白表达与病毒生长动力学后,单剂滴鼻免疫BALB/c小鼠,并评价体液免疫、黏膜免疫与细胞免疫。免疫4周后,分别用RSV A2与RSV B9320进行攻毒,通过小鼠体重变化、肺组织病毒滴度及病理评价免疫保护效果。结果:单剂滴鼻免疫PR8NAGecto/WSN能在小鼠体内产生较强的体液免疫、黏膜免疫及细胞免疫。与对照组比较,免疫组小鼠经RSV A2或B9320两个亚型病毒攻毒后肺病毒载量与肺组织病理均明显改善。结论:单剂滴鼻免疫重组PR8NAGecto/WSN疫苗可在小鼠体内诱导较强的RSV特异免疫应答与攻毒保护。本研究为新型RSV黏膜疫...

  • 标签: 呼吸道合胞病毒流感病毒病毒载体G蛋白
  • 简介:目的:基于重组流感病毒载体的呼吸道合胞病毒(RSV)疫苗的构建及在小鼠体内的免疫保护效果评价。方法:构建并拯救表达RSV A2型G蛋白胞外结构域(Gecto)的重组甲型流感病毒,将其命名为PR8NAGecto/WSN。体外验证重组病毒G蛋白表达与病毒生长动力学后,单剂滴鼻免疫BALB/c小鼠,并评价体液免疫、黏膜免疫与细胞免疫。免疫4周后,分别用RSV A2与RSV B9320进行攻毒,通过小鼠体重变化、肺组织病毒滴度及病理评价免疫保护效果。结果:单剂滴鼻免疫PR8NAGecto/WSN能在小鼠体内产生较强的体液免疫、黏膜免疫及细胞免疫。与对照组比较,免疫组小鼠经RSV A2或B9320两个亚型病毒攻毒后肺病毒载量与肺组织病理均明显改善。结论:单剂滴鼻免疫重组PR8NAGecto/WSN疫苗可在小鼠体内诱导较强的RSV特异免疫应答与攻毒保护。本研究为新型RSV黏膜疫

  • 标签: 呼吸道合胞病毒流感病毒病毒载体G蛋白
  • 简介:

  • 标签:
  • 简介:

  • 标签: