简介：Theplasmamembrane(PM)isacomplexenvironmentconsistingof>700speciesoflipidsandmanydifferenttypesofmembrane-associatingproteins.Theselipidsandmembraneproteinsaredistributedheterogeneouslyintonanometer-sizeddomains,callednanoclusters.ThelateralspatialsegregationinthePMgivesrisetodifferentcurvatureandlipidcomposition,whichdeterminestheefficiencyofeffectorbindingandsignaltransmission.Here,wedescribeanelectronmicroscopy(EM)-spatialmappingtechniquetoquantifytheextentofnanoclustersformationinthePM.Thenano-assembliesinthePMarequantifiedviaexpressingtheGFP-taggedproteinsorlipid-bindingdomainsinthecells,whicharethenimmunolabeledwiththegoldnanoparticlespre-coupledtotheanti-GFPantibody.ThegoldnanoparticlesarevisualizedviathetransmissionEMathighmagnification.ThestatisticalanalysisoftheRipley'sK-functioncalculatesthespatialdistributionofthegoldnanoparticles.Importantspatialparameters,suchastheextentofnanoclustering,theclusteredfraction,thenumberofproteinspercluster,theoptimalsizeofananocluster,andthenumberofproteinslocalizedtothePM,canbecalculated.Furtherdetailedaggregationpattern,suchasthepopulationsofmonomers,dimers,trimers,andhigherorderedoligomers,canalsobeextractedfromthespatialanalysis.TheEM-bivariateanalysisquantifiestheextentofco-localizationbetweentwodifferentcomponentsinthePMandprovideskeyinformationontheprotein-proteinandtheprotein-lipidinteractionsoveralong-distancescalefrom8to240nm.

简介：Objective:Laryngealreconstructionisneededtopreservelaryngealfunctioninpatientswhohaveundergoneextensiveverticalorfrontalpartiallaryngectomy.However,theprocedureremainsadifficultchallenge.Severalreconstructiontechniqueshavebeendescribed,butthesetechniquesposerisksofcomplicationssuchaslaryngealstenosis.Thisstudyaimedtoevaluatethepostoperativecourseandfunctionaloutcomesofanewtechniquethatcombinedamuscle-pediclehyoidboneandathyrohyoidflapduringlaryngealreconstructionaftertumorresection.Methods:Fourpatientsunderwentextensiveverticalpartialorfrontalpartiallaryngectomyforcancer.Aftertumorresection,laryngealreconstructionwasperformedusingtheproposedtechnique.Postoperativerecoverytime,complications,andoncologicresultswereevaluated.Results:Thefourpatientsweresuccessfullytreatedwiththeproposedtechnique.Nodyspnea,dysphagia,ordeathoccurredduringthepostoperativecourse.Decannulationwasperformedafteramedianof3days.Theaveragepostoperativehospitalstaywas7days.Short-termpostoperativefunctionalrecoverywasnormal.Nolaryngealstenosisortumorrecurrencewasobservedinanyofthefourpatientsafterafollow-upperiodofmorethan24months.Conclusion:Thecombinationofthemuscle-pediclehyoidboneandthethyrohyoidflapisareliableprocedureforlaryngealreconstructionafterextensiveverticalpartialorfrontalpartiallaryngectomy.

简介：Linearpolysiloxaneswithpendantbenzo-15-crown-5moietieshavebeensynthesizedfrom3′-or4′-allylbenzo-15-C-5viahydrosilylationofmethyldichlorosilane,followedbypolycondensationwithsilanol-terminatedpolydimethylsiloxane.Itwasfoundthatthepolysiloxanescouldbeusedascarriersinbulkliquidmembranetotransportions.Factorsinfluencingtransportratehavebeeninvestigated.

简介：Introduction:Middleearvolume(MEV)isaclinicallyrelevantparameteracrossmiddleeardiseases.MEVvaluesbetweenthesetechniqueshaveneverbeforebeentestedforagreementinearswithperforatedtympanicmembranes(TMs).Methods:Middleearswereidentifiedfrom36patientsranging18e89yearsofagewithTMperforationswhounderwenttympanometryandtemporalbonecomputedtomography(CT)between2005and2015.MEVscalculatedbybothtympanometryandthree-dimensionalvolumereconstruction(3DVR)wereanalyzedforagreementusingBlandAltmanplots.Thedifferencesbetweentympanometricand3DVRMEVvaluesforeachgivenmiddleearwerecharacterizedacrossMEVquartiles(1?smallest;4?largest)andacrossincreasingstatesofmiddleeardiseaseusingKruskaleWallisandWilcoxontestingwithBonferronicorrection.Results:BlandAltmanplotsdemonstratedsignificantdisagreementbetweenMEVmeasurementtechniques.Differencesbetweentympanometric(T)and3DVRMEVvaluesweresignificantlygreaterwithincreasingaverage(i.e.(Tt3DVR)/2))MEVperlinearregression(p<0.0001).SignificancewasdemonstratedbetweenfourthandfirstaverageMEVquartiles(p?0.0024),fourthandsecondquartiles(p?0.0024),thirdandfirstquartiles(p?0.0048),andthirdandsecondquartiles(p?0.048).AbsoluteMEVdifferencewasnotsignificantlydifferentacrossvaryingstatesofmiddleeardisease(p?0.44).Conclusion:Statisticallyandclinicallysignificantdisagreementwasdemonstratedbetweentympanometricand3DVRMEVvalues.StudiesthatvaryinMEVestimationtechniquesmaybeexpectedtodemonstratesignificantlydifferentresults.ThesepreliminaryresultssuggestthatcliniciansshouldendeavortoseekfurtherconfirmationwheninterpretinghightympanometricMEVvalues.

简介：TocloneandexpresstherecombinantoutermembraneproteinTp0453ofTreponemapallidumandtoanalyzetheimmuno-reactivityandimmunogenicityoftheexpressedprotein,theimmuno-dominantepitopeoftheTp0453wasamplifiedfromthecompletegenomeofT.pallidumbyPCR,subclonedintoexpressionvectorpQE32togeneratetherecombinantplasmidpQE32/Tp0453,thenexpressedinE.coliM15andanalyzedbySDS/PAGEandWesternblotting.ThefusionproteinexpressedwaspurifiedwithNi-NTAaffinitychromatography.Itsimmuno-reactivitywasassayedbyindirectELISA,andtheimmunogenicitywasdeterminedbyimmunizationwiththisfusionproteininNewZealandrabbits.Inthepresentstudy,afusionproteinofmolecularweightabout32kDawasobtained.AsdemonstratedbyWesternblotting,therecombinantproteincouldreactspecificallywithpositiveIgGseraofpatientswithsyphilis,andtheantibodiesagainstT.palliduminhumanseraweresuccessfullydetectedbyindirectELISA.BoththesensitivityandspecificityofELISAbasedontheTp0453fusionproteinaswere100%(30/30)whendetectedwithcontrolsera.IncomparisonwiththeresultsofIgGELISAwiththoseofTPPA.ItwasfoundthatthesensitivityofELISAwas96.8%andthespecificitywas100%.ThedifferenceofELISAandTPPAwasnotsignificant,andtheconcordanceofresultsbetweenELISAandTPPAwas98.2%.Inaddition,specifichumoralresponsescouldbeelicitedbyimmunizationwiththerecombinantfusionproteininNewZealandrabbitswithaspecificantibodytiterof1:1280after3successivedosesofimmunization.Theseresultsdemonstratethattheexpressedrecombinantfusionproteinshowsexcellentimmuno-competenceandprovidefoundationtodevelopaquickdiagnostickidappliedtodetectthepresenceofT.palliduminfections.

简介：Surfacemodificationofpolypropylenemicroporousmembrane(PPMM)wasperformedbyatmosphericpressuredielectricbarrierdischargeplasmaimmobilizationofN,Ndimethylaminoethylmethacrylate(DMAEMA).Structuralandmorphologicalchangesonthemembranesurfacewerecharacterizedbyattenuatedtotalreflection-Fouriertransforminfraredspectroscopy(FT-IR/ATR),X-rayphotoelectronspectroscope(XPS)andfieldemissionscanningelectronmicroscopy(FE-SEM).Watercontactanglesofthemembranesurfaceswerealsomeasuredbythesessiledropmethod.Resultsrevealthatboththeplasma-treatingconditionsandtheadsorbedDMAEMAamounthaveremarkableeffectsontheimmobilizationdegreeofDMAEMA.Peroxidedeterminationby1,1-diphenyl-2-picrvlhydrazyl(DPPH)methodverifiestheexsistenceofradicalsinducedbyplasma,whichactivizetheimmobilizationreaction.PurewatercontactangleonthemembranesurfacedecreasedwiththeincreaseofDMAEMAimmobilizationdegree,whichindicatesanenhancedhydrophilicityforthemodifiedmembranes.Theeffectsofimmobilizationdegreesonpurewaterfluxeswerealsomeasured.Itisshownthatpurewaterfluxesfirstincreasedwithimmobilizationdegreeandthendecreased.Finally,permeationofbovineserumalbumin(BSA)andlysozymesolutionweremeasuredtoevaluatetheantifoulingpropertyoftheDMAEMA-modifiedmembranes,fromwhichitisshownthatbothhydrophilicityandelectrostaticrepulsionarebeneficialformembraneantifouling.

简介：ObjectivesToinvestigatethechangeandclinicalsignificanceofclopidogrelonplateletmembraneCD40Lincoronaryarterydiseasepatientsbeforeandafterpercutaneouscoronaryintervention(PCI).Methods30caseswhowerediagnosiscoronaryarterydiseases(CAD)bycoronaryangiography,meanage56±9yearsold.Allthepatientswhohadnoantiplateletaggregationcontraindication,weretreatedwithstandardantianginapectorisdrugs.BeforePCI,allthepatientstookclopidogrel75mgperday.ActivatedplateletmembraneCD40LexpressratewasmeasuredbyflowcytometrybeforeandafterPCI6hours.ResultsActivatedplateletmembraneCD40Lexpressratewere3.73±2.15and2.46±0.90,respectivelyin30patientsbeforeandafterPCI6hours.ActivatedplateletmembraneCD40LexpressratewassignificantlydecreaseafterPCI6hoursthanthatbeforePCI(P<0.01).ConclusionsClopidogrelhassignificanceeffectonplateletmembraneCD40LincoronaryarterydiseasepatientsundergoingPCI.Clopidogrelcansuppressionplateletactivationandpreventthromboembolismeventoccurrence.

简介：Carbondioxidetransformationtofuelsorchemicalsprovidesanattractiveapproachforitsutilizationasfeedstockanditsemissionreduction.Herein,wereportagas-phaseelectrocatalyticreductionofCO_2inanelectrolyticcell,constructedusingphosphoricacid-dopedpolybenzimidazole(PBI)membrane,whichallowedoperationat170°C.Pt/CandPtMo/CwithvariableratioofPt/Mowerestudiedasthecathodecatalysts.TheresultsshowedthatPtMo/CcatalystssignificantlyenhancedCOformationandinhibitedCH_4formationcomparedwithPt/Ccatalyst.CharacterizationbyX-raydiffraction,X-rayphotoelectronspectroscopyandtransmissionelectronmicroscopyrevealedthatmostMospeciesexistedasMoO_3inPtMo/CcatalystsandtheinteractionbetweenPtandMoO_xwaslikelyresponsiblefortheenhancedCOformationratealthoughthesebicomponentcatalystsingeneralhadalargerparticlesizethanPt/Ccatalyst.

简介：多功能的cytokineinterleukin-1(IL-1)的生物活动被它的受体调停。这研究的目的是决定一个协会是否在1和2受体基因(IL1R1和IL1R2)和表示在mononuclear房间的subpopulations或可溶的IL-1受体的浆液层次上膜界限IL1Rs铺平的IL-1类型在单个核苷酸多型性(SNP)之间存在。这被观察有在SNPrs2234650的遗传型TT的健康个人:C>；T有在他们的表面上表示IL1R1的未经触动的CD14+单核白血球的一个更低的百分比。SNPrs4141134:T>；在IL1R2的C也与表示IL1R2的未经触动的CD3+T房间的百分比被联系了。而且，带SNPrs4141134的CC等位基因的个人:T>；C和SNPrs2071008的TT等位基因:T>；在IL1R2的G在刺激的lipopolysaccharide(LPS)在CD14+单核白血球的表面上有IL1R2s的更低的密度PBMC文化。在摘要，这研究证明IL-1受体基因多型性能是在免疫能力的房间上影响膜界限IL-1受体(IL1R)的表示的因素之一。

简介：AbstractHolmium laser lithotripsy (HLL) is one of the common surgical methods for urolithiasis. It causes minor surgical trauma, but complications are not rare. Extracorporeal membrane oxygenation (ECMO) treatment of sepsis is common, but venoarterial (VA)-ECMO treatment of urosepsis has not been reported yet. In this article, we reported a 67-year-old female patient with refractory septic shock caused by HLL under percutaneous nephroscope, involving breathing, heart, kidney and other organs, and organs support treatment was ineffective for the patient. Finally, we successfully treated the patient under VA-ECMO with continuous renal replacement therapy (CRRT). Combined ECMO and CRRT may provide a solution for addressing refractory sepsis. Here we present the case and review relevant literature, so as to provide a treatment strategy for patients with refractory urogenic sepsis and to reduce the mortality rate.

简介：ObjectiveTostudyexpressionofadenoviral-mediatedHath1-EGFPgeneintheguineapigcochleaaftertransferthroughintactroundwindowmembrane(RWM),andtoassessitseffectsonhearing.MethodsTwentyadultguineapigswereused,ofwhich:12weresurgicallyinoculatedwithAd-Hath1-EGFPinthebonygrooveofroundwindowniche,and8withartificialperilymph.Auditorybrainstemresponse(ABR)thresholdsweredeterminedinallanimalsbeforeand5daysaftersurgery.Onpost-surgeryday5andday14,animalsweresacrificedandwholemountsofcochleaandfrozensectionswereexamined.ResultsABRtestsshowednosignificantchangeofhearingafterthesurgery.StrongfluorescencestaininginthecochleaewasseeninAd-Hath1-EGFPgroups.Thehighestlevelsofgeneexpressionwereseeninthepost-surgeryday5groupwithlittledecreaseonpost-surgeryday14.Thecontralateralcochleaandthoseinthecontrolgroupswerefreeoffluorescencestaining.ConclusionThetransgenicHath1-EGFPcanbeeffectivelydeliveredintotheinnerearthroughintactRWM,inanatraumaticmanner.

简介：AbstractBackground:Intracerebral hemorrhage (ICH) is one of the most severe complications during veno-venous extracorporeal membrane oxygenation (VV-ECMO). This study aimed to determine the risk factors for ICH and mortality in such patients.Methods:We analyzed the clinical data of 77 patients who received VV-ECMO due to severe respiratory failure from July 2013 to May 2019 at China-Japan Friendship Hospital. Demographical data, laboratory indices, imaging characteristics, and other clinical information were collected. Multivariable logistic regression analyses were performed to identify risk factors for ICH and mortality.Results:Of 77 patients, 11 (14.3%) suffered from ICH, and 36 (46.8%) survived. The survival rate was significantly lower (18.2% [2/11] vs. 51.5% [34/66], P = 0.040) in patients with ICH than in those without ICH. Multivariable analysis revealed that factors independently associated with ICH were diabetes mellitus (adjusted odds ratio [aOR]: 12.848, 95% confidence interval [CI]: 1.129-146.188, P = 0.040) and minimum fibrinogen during ECMO (aOR: 2.557, 95% CI: 1.244-5.252, P = 0.011). Multivariable analysis showed that factors independently associated with mortality were acute hepatic failure during ECMO (aOR: 9.205, 95% CI: 1.375-61.604, P = 0.022), CO2 retention before ECMO (aOR: 7.602, 95% CI: 1.514-38.188, P = 0.014), and minimum platelet concentration during ECMO (aOR: 0.130, 95% CI: 0.029-0.577, P = 0.007).Conclusions:Diabetes mellitus and minimum fibrinogen concentration during ECMO are risk factors for ICH in patients with severe respiratory failure managed using VV-ECMO. This indicated that anticoagulants use and nervous system monitoring should be performed more carefully in patients with diabetes when treated with VV-ECMO due to severe respiratory failure.

简介：Pt/WO3/CnanocompositeswithparallelWO3nanorodsweresynthesizedandappliedasthecathodecatalystforprotonexchangemembranefuelcells(PEMFCs).Electrochemicalresultsandsinglecelltestsshowthatanenhancedactivityfortheoxygenreductionreaction(ORR)isobtainedforthePt/WO3/CcatalystcomparedwithPt/C.ThehighercatalyticactivitymightbeascribedtotheimprovedPtdispersionwithsmallerparticlesizes.ThePt/WO3/Ccatalystalsoexhibitsagoodelectrochemicalstabilityunderpotentialcycling.Thus,thePt/WO3/CcatalystcanbeusedasapotentialPEMFCcathodecatalyst.

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简介：在在甲醇的9-vinyladenine和嘧啶库胸腺嘧啶之间的有约束力的机制与紫外可见的spectrophotometric方法被学习。把学习基于这，用象一个模板分子的胸腺嘧啶，是的9-vinyladenine是的新奇功能的单体和diethylene乙二醇dimethacrylate新cross-linker，特定的diethylene乙二醇基于dimethacrylate的分子地印的聚合的膜在纤维素支持上被准备。然后，结果地聚合的膜形态学与扫描电子显微镜学被设想，它的permselectivity作为底层用胸腺嘧啶，uracil，cytosine，腺嘌和guanine被检验。这结果证明印与diethylene乙二醇dimethacrylate准备的聚合的膜比另外的nucleic酸底为模板分子胸腺嘧啶和它的最佳的模拟uracil展出了更高的运输能力。膜也比作为cross-linker与乙烯乙二醇dimethacrylate做的印的膜呈现了更高的permselectivity。包括五nucleic酸的混合物什么时候基于胸腺嘧啶，uracil，cytosine，腺嘌和guanine，通过了diethylene乙二醇基于dimethacrylate的印胸腺嘧啶的聚合的膜，为模板分子胸腺嘧啶和它的最佳的模拟uracil的膜的识别被表明。因为它的高选择，作为cross-linker与diethylene乙二醇dimethacrylate准备的分子地印的膜可能对DNA的绝对hydrolysates的胸腺嘧啶试金或在生物样品的RNA的绝对hydrolysates的uracil试金适用，这被预言。

简介：Brassinosteroids(BR)被transmembrane受体和戏察觉在植物生长和开发的重要角色，以及响应环境刺激的房间。transmembrane受体BRI1能直接绑在brassinolide(BL)，并且BAK1与BRI1交往提高发信号的调停BRI1的BR。我们的以前的研究显示了那膜类固醇绑定蛋白质(MSBP1)1能在vitro绑在BL并且否定地涉及BR发信号。进一步阐明内在的机制，我们这里证明MSBP1明确地以一种BL独立的方式在vivo与BAK1的细胞外的领域交往。由MSBP1的增加的表示的压制的房间扩大和BR回答能被overexpressingBAK1或它的细胞内部的kinase领域恢复，建议MSBP1可以压制通过与BAK1交往发信号的BR。Subcellular本地化研究表明MSBP1和BAK1对血浆膜和endocytic泡局部性，MSBP1加速BAK1endocytosis，它导致由向内涵体转移BAK1的平衡发信号的压制的BR。确实，提高了MSBP1表示还原剂在在vivo的BRI1和BAK1之间的相互作用，表明那MSBP1在发信号的BR的早步充当一个否定因素小径。

简介：在这研究，一新(乙烯基氯化物)(PVC)poly，为Ho3+离子的膜传感器作为ionophore基于N-phenyl-2-(thiophen-2-ylmethylene)hydrazinecarbothioamide(PHC)被准备。这个传感器与阳离子的变化比较向钬离子表明了好选择和敏感，包括碱，碱的地球，转变和重金属离子。电极的反应性质上的膜作文和pH的效果被调查。详细，建议传感器展出了Nernstian行为(与20.4的一个斜坡??洀????x

简介：Basementmembranedegradationandblood-brainbarrierdamageappearaftercerebralinfarction,severelyimpactingneuronalandbrainfunctioning;however,theunderlyingpathogeneticmechanismsremainpoorlyunderstood.Inthisstudy,weinducedcerebralinfarctioninstrokepronespontaneouslyhypertensiveratsbyintragastricadministrationofhigh-sodiumwater(1.3%NaCl)for7consecutiveweeks.Immunohistochemicalandimmunofluorescenceassaysdemonstratedthat,comparedwiththenon-infarctedcontralateralhemisphere,stroke-pronespontaneouslyhypertensiveratsonnormalsodiumintakeandWistar-Kyotorats,matrixmetalloproteinase-9expression,thenumberofbloodvesselswithdiscontinuouscollagenIVexpressionandmicrovesseldensityweresignificantlyhigher,andthenumberofcontinuouscollagenIV-positivebloodvesselswaslowerintheinfarctborderzonesofstroke-pronespontaneouslyhypertensiveratsgivenhigh-sodiumwater.Linearcorrelationanalysisshowedmatrixmetalloproteinase-9expressionwaspositivelycorrelatedwiththenumberofdiscontinuouslycollagenIV-labeledbloodvesselsandmicrovesseldensityincerebralinfarctsofstroke-pronespontaneouslyhypertensiverats.Theseresultssuggestthatmatrixmetalloproteinase-9upregulationisassociatedwithincreasedregionalangiogenesisanddegradationofcollagenIV,themajorcomponentofthebasallamina,instroke-pronespontaneouslyhypertensiveratswithhigh-sodiumwater-inducedfocalcerebralinfarction.

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