简介：螺旋体serovars引起的威胁生活的感染为设计反细螺旋体病药要求需要。现在的学习包含对螺旋体的phosphoheptoseisomerase(GmhA)探索禁止者，它为lipopolysaccharide(LPS)是重要的生合成并且通过减少性的genomic途径作为一个普通的药目标被识别。GmhA模型在Modeller9v7被造。预言的模型的结构的精炼和精力最小化用艺术大师9.0被执行。精制模型可靠性通过Procheck，ProSA，ProQ和侧面3D被估计。基于底层虚拟高产量的屏蔽(VHTS)在Ligand。信息元数据库工具产生了354底层的一个内部图书馆结构的类似物。而且，从有每ligand的不同符合构造的内部图书馆的基于结构的VHTS提供了14个新奇竞争禁止者。和从VHTS获得的卓见的模型将是为开发反细螺旋体病的一个有希望的起点竞争禁止者指向LPS生合成小径。

简介：ObjectiveToscreenformutationsinmyosin-7Agene(MYO7A)inpatientswithprelingualnonsyndromichearingimpairment.Methods31sporadicpatientswithcongenitalhearingimpairmentand65patientsfrom34familieswithprelingualhereditaryhearingimpairmentinChinaweretestedinthisstudy,and100hearingnormalindividualswereusedascontrol.GenomicDNAisolatedfromwholebloodofallsubjectswassubjectedtopolymerasechainreaction(PCR)toamplifyselectedexonsofMYO7Agene.ThePCRproductsweresubsequentlyscreenedusingsinglestrandconformationalpolymorphismanalysis(SSCP)anddirectsequencingwhenthefragmentsshowedanabnormalelectrophoreticpattern.ResultsGgAtransitionatposition617inexon7,whichwouldproduceanA206Gaminoacidsubstitution,wasdetectedintwopatientsbutinnoneoftheunaffectedmembersinthefamilies.ThisheterozygousmissensemutationhappenedwithinahighlyconservedheptapeptidesequenceofMYO7Aprotein,andiscloselyrelevanttopreligualnonsyndromicdeafness.ConclusionsTheA206Gsubstitutionispossiblyanewmutationtocausepreligualnonsyndromichearingimpairment.Ourresultsprovideevidencethatexon7ofMYO7Aisamutationalhotspotingenetichearingimpairment.

简介：表示顺序标注的Porphyrayezoensis(EST)的genomic资源被利用识别简单顺序重复(SSR)，或微卫星。这个方法拿了为基因身份的建立或为高多型性的获得使用EST和微卫星的优点。当基因标记whenmicrosatellites被附在基因，微卫星能被使用。由生物信息学分析揭示了，1162从21954颗ESTscontained微卫星和聚合分析显示984这些EST掉进112contigs，当另外的178EST是单条时。290唯一的包含SSR基因的一个总数被识别。AACSSR是微卫星的最人口多的类型。在所有微卫星之中占优势的GC富有的microsatelliteswere。

简介：Objective:Hepatocellularcarcinoma(HCC)isaleadingcauseofcancer-relateddeaths.NovelserumbiomarkersarerequiredtoincreasethesensitivityandspecificityofserumscreeningforearlyHCCdiagnosis.ThisstudyemployedaquantitativeproteomicstrategytoanalyzethedifferentialexpressionofserumglycoproteinsbetweenHCCandnormalcontrolserumsamples.Methods:Lectinaffinitychromatography(LAC)wasusedtoenrichglycoproteinsfromtheserumsamples.Quantitativemassspectrometricanalysiscombinedwithstableisotopedimethyllabelingand2Dliquidchromatography(LC)separationswereperformedtoexaminethedifferentiallevelsofthedetectedproteinsbetweenHCCandcontrolserumsamples.Westernblotwasusedtoanalyzethedifferentialexpressionlevelsofthethreeserumproteins.Results:Atotalof2,280proteingroupswereidentifiedintheserumsamplesfromHCCpatientsbyusingthe2DLC-MS/MSmethod.Upto36proteinswereup-regulatedintheHCCserum,whereas19proteinsweredown-regulated.Threedifferentialglycoproteins,namely,fibrinogengammachain(FGG),FOS-likeantigen2(FOSL2),andα-1,6-mannosylglycoprotein6-β-N-acetylglucosaminyltransferaseB(MGAT5B)werevalidatedbyWesternblot.Allthesethreeproteinswereup-regulatedintheHCCserumsamples.Conclusion:AquantitativeglycoproteomicmethodwasestablishedandprovenusefultodeterminepotentialnovelbiomarkersforHCC.

简介：Whitespotsyndromevirus(WSSV)-resistantmolecularmarkerswerescreenedfromtheselectivelybrednewvariety‘HuanghaiNo.2’ofFenneropenaeuschinensisusingunlabeled-probehigh-resolutionmelting(HRM)technique.AftertheartificialinfectionwithWSSV,thefirst96deadshrimpsandthelast96survivingshrimpswerecollected,representingWSSV-susceptibleand-resistantpopulations,respectively.Thegenotypesatwell-developed39singlenucleotidepolymorphisms(SNPs)lociwereobtained.AsrevealedintheChi-squaretest,3SNPs,genotypeA/AofcontigC364-89AT,genotypeA/AofC2635-527CAandgenotypeC/TofcontigC12355-592CT,werepositivelycorrelatedwithdisease-resistancetraits.Other2SNPs,genotypeG/GofcontigC283-145AGandgenotypeC/CofcontigC12355-592CT,werenegativelycorrelated.Moreover,analysiswithBlastXprogramfordisease-resistantSNPsindicatedthat3contigs,Contig283,Contig364andContig12355,matchedtothefunctionalgenesofeffectorcaspaseofPenaeusmonodon,peptidetransporterfamily1-likeprotein,and40SribosomalproteinS2ofPercaflavescenswithhighsequencesimilarity.Theresultswillbehelpfultoprovidetheoreticalandtechnicalsupportsformolecularmarker-assistedselectivebreedingofF.chinensis.

简介：Objective:Toevaluatetheefficacyandfeasibilityofscreeningprocedureforuppergastrointestinalcancerinbothhigh-riskandnon-high-riskareasinChina.Setting:Sevencities/counties,representingthreeeconomical-geographicalregions(Eastern,CentralandWestern)inChina,wereselectedasscreeningcenters:threeinhigh-riskareasandfourinnon-high-riskareas.Participants:Villages/communitiesinthesesevencentersregardedasclusterswererandomlyassignedtoeitherinterventiongroup(screeningbyendoscopicexamination)orcontrolgroup(withnormalcommunitycare)ina1:1ratiostratifiedbyeachcenter.Eligibleparticipantsarelocalresidentsaged40–69yearsintheselectedvillages/communitieswithnohistoryofcancerorendoscopicexaminationinthelatest3yearswhoarementallyandphysicallycompetent.Thosewhoarenotwillingtotakeendoscopicexaminationorareunwillingtosigntheconsentformareexcludedfromthestudy.Totally140,000participantswillbeenrolled.Interventions:Inhigh-riskareasofuppergastrointestinalcancer,allsubjectsinscreeninggroupwillbescreenedbyendoscopy.Innon-high-riskareas,30%ofthesubjectsinscreeninggroup,identifiedthroughasurvey,willbescreenedbyendoscopy.Primaryandsecondaryoutcomemeasures:Theprimaryoutcomeisthemortalitycausedbyuppergastrointestinalcancer.Thesecondaryoutcomesincludedetectionrate,incidencerate,survivalrate,andclinicalstagedistribution.Additionaldataonqualityoflifeandcost-effectivenesswillalsobecollectedtoanswerimportantquestionsregardingscreeningeffects.Conclusions:ScreeningstrategyevaluatedinthoseareaswithpositivefindingsmaybepromotednationallyandappliedtothemajorityofChinesepeople.Ontheotherhand,negativefindingswillprovidescientificevidenceforabandoningatestandshiftingresourceselsewhere.Trialregistration:ThestudyhasbeenregisteredwiththeProtocolRegistrationSysteminChineseClinicalTrialRegistry.

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简介：AbstractBackground:Previous studies have revealed that diabetes mellitus (DM) promotes disease progress of gastric cancer (GC). This study aimed to further investigating whether DM advanced lymph nodes (LNs) metastasis in GC.Methods:The clinicopathologic data of GC patients with >15 examined LN (ELN) between October 2004 and December 2019 from a prospectively maintained database were included. The observational outcomes included the number (N3b status) and anatomical distribution (N3 stations) of metastatic LN (MLN).Results:A total of 2142 eligible patients were included in the study between October 2004 and December 2019. N3 stations metastasis (26.8% in DM vs. 19.3% in non-DM, P = 0.026) and N3b status (18.8% in DM vs. 12.8% in non-DM, P = 0.039) were more advanced in the DM group, and multivariate logistic regression analyses confirmed that DM was an independent factor of developing N3 stations metastasis (odds ratio [OR] = 1.771, P= 0.011) and N3b status (OR= 1.752, P= 0.028). Also, multivariate analyses determined DM was independently associated with more MLN (β = 1.424, P = 0.047). The preponderance of N3 stations metastasis (DM vs. non-DM, T1-2: 2.2% vs. 4.9%, T3: 29.0% vs. 20.3%, T4a: 38.9% vs. 25.8%, T4b: 50.0% vs. 36.6%; ELN16-29: 8.6% vs. 10.4%, ELN30-44: 27.9% vs. 20.5%, ELN ≥ 45: 37.7% vs. 25.3%), N3b status (DM vs. non-DM, T1-2: 0% vs. 1.7%, T3: 16.1% vs. 5.1%, T4a: 27.8% vs. 19.1%, T4b: 44.0% vs. 28.0%; ELN16-29: 8.6% vs. 7.9%, ELN30-44: 18.0% vs. 11.8%, ELN ≥ 45: 26.4% vs. 17.3%), and the number of MLN (DM vs. non-DM, T1-2: 0.4 vs. 1.1, T3: 8.6 vs. 5.2, T4a: 9.7 vs. 8.6, T4b: 17.0 vs. 12.8; ELN16-29: 3.6 vs. 4.6, ELN30-44: 5.8 vs. 5.5, ELN ≥ 45: 12.0 vs. 7.7) of DM group increased with the advancement of primary tumor depth stage and raising of ELN.Conclusions:DM was an independent risk factor for promoting LN metastasis. The preponderance of LN involvement in the DM group was aggravated with the advancement of tumor depth.

简介：BackgroundPatientswithunstableanginapectorisanddiabetesmellitusareverycommon.Whentheyreceiveinterventionaltherapy,contrastagentscancauserenalinjuryandplateletactivation.Alprostadilhasbeenshowntodecreaseinflammationandmicrovascularresistance.Thereisnoanyreportontheprotectioneffectsofalprostadilonrenalinjuryandtheplateletactivationduringcardiacinterventionaltherapy.MethodsAtotalof57patientsweredividedintotwogroups,alprostadilgroup(n=35)andnormalgroupn=22).BUN(bloodureanitrogen)andSCr(serumcreatine)weremeasuredastherenalfunctionparameters.GFR(glomerularfiltrationrate)wascalculatedbytheMDRDformula.Plateletparameters:PLT(plateletcount,109),PDW(plateletdistributionwidth,fL),MPV(meanplateletvolume,fL),PLCR(largeplateletscaleratio,%)weremeasuredastheplateletactivationindex.TtestandChi-squaretestwereusedasstatisticalmethods,andP<0.05wasconsideredstatisticallysignificant.ResultsInthealprostadilgroup,SCrwas71.1±13.8μmol/Land65.9±12.6μmol/L,beforeandafterinterventionaltherapy(P<0.05).BUNwas5.68±1.79mmol/Land3.86±1.19mmol/L(P<0.001),beforeandafterinterventionaltherapy.Intheplatelettests,MPVwasseemedtobetheonlyusefulplateletparameter,beforeandafterinterventionaltherapy(11.8±1.8fLand11.1±1.2fL,P<0.05),whilethePLT(109/L),PDW(fl),PLCR(%)didn'tshowanysignificantdifference.Inthenormalgroup,allthesetests'resultsdidn'tsignificantlychangeafterinterventionaltherapy.ConclusionsThepatientswiththeunstableanginapectorisanddiabetesmellitushaveontheriskofrenalfunctiondamageandplateletactivationwhenundertakingcardiacinterventionaltherapy.Alprostadilprotectsrenalfunctionandalleviatesplateletactivation.

简介：ObjectiveTostudytheefficacyandsafetyoftheJiangTangFangLongJiaoNang(HypoglycemicAnti-DeafnessCapsule)intreatingnon-insulindependentdiabetesmellituswithhearingloss.MethodsTwohundredninetysixpatientswithnon-insulindependentdiabetesmellitusandhearinglosswererandomlyassignedtoatreatmentgroup(n=164,208ears)andacontrolgroup(n=132,184ears).PatientsinthetreatmentgroupweretreatedwiththeJiangTangFangLongJiaoNangandsupplementherbalpreparationsasindicatedbytraditionalChinesemedicinedialecticalassessment,whilecontrolpatientsreceivedglibenclamideandconventionaltreatmentsfordeafness.Hearing,fastingbloodglucose(FBG),post-prandialbloodglucose(PBG),24hoururinesugar,plateletfunctionindices,bloodsuperoxidedismutase(SOD)andlipidperoxides(LPO)levels,andsymptomimprovementwerecomparedbetweenthetwogroups.ResultsTherateofhearingimprovementwas56.7%forthetreatmentgroupand26.6%forthecontrol.FBG,PBGand24hoururinesugarimprovedinbothgroups,butthelasttwoweresuperiorinthetreatmentgroupcomparedtothecontrol.Symptomsimprovementwasalsosuperiorinthetreatmentgroupcomparedtothecontrol.InpatientsreceivingJiangTangFangLongJiaoNangtreatment,plateletfunctionindices,SODandLPOwereallimproved,whileonlyLOPimprovementwasnoticedincontrolpatients.Noacuteorlong-termtoxicitywasdemonstratedfortheJiangTangFangLongJiaoNanginanimaltests.TheJiangTangFangLongJiaoNangloweredbloodglucoseandserumtriglyceridesinaratmodelofalloxan-induceddiabetes.ConclusionTheJiangTangFangLongJiaoNangiseffectiveinimprovinghearinganddiabeticindicesindiabeticpatientswithdeafness,withoutsignificantsideeffects.

简介：Therearefewpopulation-baseddataininvestigatingtheimpactofdiabetesonchemotherapyadverseeffectsandtreatmentoutcomesofnon-metastaticbreastcancer.Thepurposeofthisstudyistoevaluatewhetherdiabetesaffectsthepatternsofuseinchemotherapy,toxiceffectsofchemotherapy,andtreatmentoutcomesfornon-metastaticbreastcancerinTaiwan.Thestudyresultscanprovidephysiciansformakingadecisionwhetherornottousechemotherapybasedontheindividualpatients’condition.

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简介：During2004,atotalof124batchesofHIVantibodyELISAsfromdomesticandoverseasmanufacturers,comprisingapproximately60milliontests,weretestedforqualityandreleasedforscreeningbloodinChina.Theinter-andintra-batchvariation,specificity,andsensitivitywereevaluatedusingalaboratorypanelandclinicalsamples.Theinter-batchvariationwaslessthan15%andonly2of12assayshadintra-batchvariationoflessthan20%for4dilutionsofacontrolspecimen.257samplesconfirmedpositiveforHIVantibodyand4826negativesamplesfromdifferentregionsinChinawereusedtoevaluatethesensitivityandspecificityoftheassays.Theresultsshowedthatthesensitivityisintherangefrom93.7%to100%forassayssampleddirectlyfromthemanufacturers,and91.4%-99.6%forthoseretrievedfromtheconsumers;thespecificitywasintherangefrom97.88%to99.97%.ThetestingenvironmentmayvaryindifferentregionsofChina.Therefore,manufacturersshouldproviderobustassaystosatisfytherequirementsofthesediverseenvironments,andespeciallyreducetheintra-assayvariationandimprovethestabilityofthekits.

简介：Intheterahertz(THz)band,theinherentshakeofthehumanbodymaystronglyimpairtheimagequalityofabeamscanningsinglefrequencyholographysystemforpersonnelscreening.Torealizeaccurateshakecompensationinimagingprocessing,itisquitenecessarytodevelopahigh-precisionmeasuresystem.However,inmanycases,differentpartsofahumanbodymayshaketodifferentextents,resultingingreatlyincreasingthedifficultyinconductingareasonablemeasurementofbodyshakeerrorsforimagereconstruction.Inthispaper,abodyshakeerrorcompensationalgorithmbasedontherawdataisproposed.Toanalyzetheeffectofthebodyshakeontherawdata,amodelofechoedsignalisrebuiltwithconsideringboththebeamscanningmodeandthebodyshake.Accordingtotherebuiltsignalmodel,wederivethebodyshakeerrorestimatedmethodtocompensateforthephaseerror.Simulationonthereconstructionofpointtargetswithshakeerrorsandproof-of-principleexperimentsonthehumanbodyinthe0.2-THzbandarebothperformedtoconfirmtheeffectivenessofthebodyshakecompensationalgorithmproposed.

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简介：ObjectiveChronictinnitusisahighlyprevalentconditionandhasbeenhypothesizedtoresultfromaninnatedisturbanceincentralnervousserotonergictransmission.Giventhefrequentcomorbiditywithmajordepressionandanxiety,wearguethatcandidategenesforthesedisordersarelikelytooverlap.Thepresentstudyaddressesthegeneencodingforthe5-HT1Areceptorasaputativeriskfactorfortinnitus.MethodsIn88subjectswithadiagnosisofchronicsubjectivetinnituswhounderwentadetailedneurootologicalexamination,theentire5-HT1AgenewasamplifiedusingoverlappingPCRproducts.Ampliconswerecustomsequencedbidirectionallyandwerescreenedforvariantsinmultiplealignmentsagainstthehumangenomereference.ResultsWeidentifiedasynonymousC>Texchangeatresidue184(Pro)in7/88subjects,butdetectednomissensevariantsinthepopulationunderstudy.Specifically,thefollowingresidueswerefullyconserved:16(Pro),22(Gly),28(Ile),98(Val),220(Arg),267(Val),273(Gly),and418(Asn).DiscussionThepresentdatacountagainstthecausationofchronictinnitusbyachangeinthe5-HT1Areceptor'saminoacidsequence.However,theallelefrequencyforthe184Prominorallele(0.04)reachedtwicethefrequencyreportedincontrolcohortsfromthesameethnicity.Additionalinvestigationsareinvitedtoclarifytheroleofthe5-HT1Apolymorphisminlargersamples,andtocontrolforcomorbidaffectivedisorders.

简介：AIMDevelopmentaltoxicitytestingtocurrentinternationalguidelinesinvolvesexposureofpregnantanimals,mostlyratsandrabbits,andsubsequentassessmentoftoxiceffectsintheirfetuses.Alternativemethodshavebeendevelopedsince1980s.Themostcomplexassaysinthisareamakedistinctiveandextensiveuseofisolatedpostimplantationrodentembryoswhichareculturedinvitroduringthephaseofmajororganogenesis.Theobjectiveofthisstudyistoestablishaninvitroembryotoxicitytest----basedonpostimplatation9.5dwholeembryoculture(WEC)and3T3celltoxicity.Thepossibilitiesfortheirapplicationasembryotoxicityscreenshavebeeninvestigated.

简介：瞄准：为了由基于人口的盒子控制为前列腺癌症调查可能的关联因素，在中国学习。方法：我们执行了在长春的前列腺癌症的集体屏蔽，中国，使用前列腺特定的抗原由日本国际合作机构帮助了。从1998年6月到2000年12月，超过50岁的3940个人被屏蔽。这些，29个人与前列腺癌症被诊断。我们选择了28个案例并且与低前列腺特定的抗原价值的控制匹配他们(<4.1ng/mL)在1：10根据雇用的年龄和地方。饮食和前列腺癌症的盒子控制研究然后被执行。结果：在为教育的调整以后，身体质量索引(BMI)，吸烟，白酒消费，婚姻和饮食，大豆产品的吸入被发现是相反地与前列腺癌症有关。在不同的天每星期消费了大豆产品多于两次的人有一多变量机会比率(或)0.38(95%信心间隔[CI]，0.13-1.12)。另外，每天消费了大豆产品多于一次的人有一多变量或0.29(95%CI，0.11-0.79)与消费了大豆产品的人相比不到一次每星期。为趋势的P是0.02，它显示出有效差量。在为任何奶店食物的P趋势没有有效差量。甚至当我们由另外的标准匹配盒子和控制时，我们发现大豆食物是与前列腺癌症联系的唯一的预防因素。结论：我们的学习建议大豆的那消费，在亚洲的最流行的食物之一，将减少前列腺癌症的风险。

简介：Themajorityofrice(OryzasativaL.)producedinthesouthernUSAisdrill-seededandgrownunderupland-likeconditionsbecausepermanentfloodingisestablishedafterthefour-leafstage.Therefore,riceduringtheseedlinggrowthstagewillbesubjectedtovariablesoilmoisturecontent.Agreenhouseexperimentwasconductedtoevaluatetheperformanceof15ricecultivarscommonlygrowninMississippiofUSAunderearly-seasonsoilmoisturestress.Twentymorpho-physiologicalparametersofriceseedlingssubjectedtothreedifferentlevels(100%,66%and33%fieldcapacity)ofsoilmoisture,from10to30daftersowing,weremeasured.Significantmoisturestress×treatmentinteraction(P<0.001)wasobservedformostoftheparameters.Further,thetotaldroughtresponseindex(TDRI)wasdevelopedtoscorethecultivarsfordroughttolerancewiththevariationfrom26.88to36.21.Accordingly,thecultivarswereclassifiedintodifferentgroupsoftolerance.ThecultivarsCL152andCL142-ARwereclassifiedastheleastandthemosttoleranttodroughtbasedonTDRIandstandarddeviation,respectively.Eventhoughbothtotalroot(R2=0.98)orshoot(R2=0.76)droughtresponsesindiceswerepositivelycorrelatedwithTDRI,roottraitswereimportantinderivingtheindices.Therefore,TDRIcouldbeusedtoselectcultivarsfordroughttoleranceinagivenenvironmentanddevelopricevarietieswithearly-seasondroughttolerance.However,furtherresearchisneededtoidentifyandcharacterizetoleranceatotherstagestoassistbreedingprogramsinrice.