简介:我们识别了米饭花的机关发展异种,称为的同样开的壳和男性无菌1(ohms1),从indicarestorer线的子孙,Zhonghui8015与60Co光线照耀对待。ohms1异种展出了开的壳并且像词根并且象palea一样组织在花药和耻辱之间的变换,它导致了显示出‘tridentatelemma’的ohms1异种小穗状花小穗。ohms1异种是完全无菌却有的60%-70%肥沃的花粉。印射的基因分析和基因证明ohms1被单个后退的基因控制,并且变异的基因对在标记KY2和KY29之间的染色体3的短手臂上的42-kb间隔印射罚款。在这个区域的四个开的读物框架的顺序分析表明异种在第五intron的最后底带了单个核苷酸转变(到G的A),它多半被通信到ohms1phynotype,在一种MIKC类型疯盒子的基因OsMADS1(LOC_Os03g11614)。进一步定序的酶消化和cDNA显示可变拼接在MADS领域或氨基酸框架为在ohms1,而是没有结构的变化的第六exon的删除负责移动出现了。另外,即时荧光灯量的PCR分析证明OsMADS1表示水平在ohms1异种显著地减少了。发展相关的基因也显著地改变了的米饭flowering因素和花的颖的表示层次。这些结果证明OsMADS1可以在米饭起一个重要作用花的机关开发,特别地在花的颖开发和小花原基区别。
简介:ThekeyforriceplantsurvivalunderNaClsaltstressismaintainingahighK+/Na+ratioinitscells.Selectionforsalttolerancericegenotypesbasedonphenotypicperformancealonewilldelayinprogressinbreeding.Useofmolecularmarkersintandemwithphysiologicalstudieswillhelpinbetteridentificationofsalttolerantriceaccessions.EightriceaccessionsalongwiththecheckDongjinwerescreenedusing1/2Yoshidasolutionwith50mmol/LNaClattheseedlingstage.TheaccessionsIT001158,IT246674,IT260533andIT291341wereclassifiedassalttolerantbasedontheirK+/Na+ratios.SeventeenSSRmarkersreportedtobeassociatedwithK+/Na+ratiowereusedtoscreentheaccessions.FiveSSRmarkers(RM8053,RM345,RM318,RM253andRM7075)coulddifferentiateaccessionsclassifiedbasedontheirK+/Na+ratios.BandingpatternoftheaccessionswasscoredcomparedtothebandingpatternofDongjin.ThestudydifferentiatedaccessionsbasedontheirassociationofK+/Na+ratiowithmolecularmarkerswhichareveryreliable.Thesemarkerscanplayasignificantroleinscreeninglargesetofricegermplasmsforsalttoleranceandalsohelpinidentificationofhigh-yieldingvarietieswithbettersalttolerance.Thesalttolerantaccessionscanbetakenforwardintodevelopingbettervarietiesbyconventionalbreedingandexploringgenesforsalttolerance.