简介：TheTK-selectedchromosome-mediategenetransferlineswereanalysedusingDNAdotblotmethod,G-11bandingandinsituhybridization.TheresultsshowedthatCMGTcanprovideawidevarietyofintermediatesizeofthetransgenomefromgreaterthan80,000kbtolessthan2,000kb.SomeoftransfectantsareintergratedintomousechromosomewhichcanbedetectedbyG-11bandingandinsituhybridization

简介：Inthelightofφ-mappingmethodandtopologicalcurrenttheory,thecontributionofdisclinationlinestofreeenergydensityofliquidcrystalsisstudiedinthesingle-elasticconstantapproximation.Itispointedoutthatthetotalfreeenergydensitycanbedividedintotwoparts.Oneistheusualdistortedenergydensityofdirectorfieldaroundthedisclinationlines.Theotheristhefreeenergydensityofdisclinationlinesthemselves,whichisshowntobecentralizedatthedisclinationlinesandtobetopologicallyquantizedintheunitofkπ/2.ThetopologicalquantumnumbersaredeterminedbytheHopfindicesandBrouwerdegreesofthedirectorfieldatthedisclinationlines,i.e.thedisclinationstrengths.FromtheLagrange'smethodofmultipliers,theequilibriumequationandthemolecularfieldofliquidcrystalsarealsoobtained.ThephysicalmeaningoftheLagrangianmultiplierisjustthedistortedenergydensity.

简介：Fieldperformanceofwhitebackedplanthopper(WBPH)-resistanceoffourphenotypeswasevaluatedinChunjiang06(CJ-06)/TN1DHricelines,whichwereexpressedbydifferentcombinationsofsuckinginhibitoryandovicidaltraitsinheritedindependentlyfromCJ-06.WBPHestablishedthehighestpopulationsinsusceptibleDHlinesthathadneithersuckinginhibitory-norovicidalresistance.Bothimmigrationandsubsequentpopulationlevelswerekeptbelowthedamage-causingdensityinthesuckinginhibitoryDHlinesevenunderaWBPHoutbreak.WBPHcouldnotbuilduppopulationsintheDHlineshavingboththesuckinginhibitoryandovicidalresistance.AlthoughWBPHimmigratedpreferentiallytonon-suckinginhibitoryDHlineswithovicidalresistance,subsequentpopulationbuildupwassignificantlysuppressed.ItwasconcludedthatthedifferentialperformancetoWBPH-resistanceinCJ-06/TN1DHlineswasprimarilyduetothesuckinginhibitorytrait,andcomplementarilytotheovicidaltrait.

简介：ObjectiveTopresentanexperimentalmethodthatallowsisolationofgreaterepithelialridge(GER)andlesserepithelialridge(LER)cellsfrompostnatalratcochleaeusingacombinatorialapproachofenzymaticdigestionandmechanicalseparationandtoinvestigatearetrovirus-mediatedgenetransfertechniqueforitspossibleutilityinimmortalizationoftheGERandLERcelllines,inanefforttoestablishaninvitromodelsystemofhaircelldifferentiation.MethodsGERandLERcellsweredissectedfrompostnatalratcochleaeandimmortalizedbytransferringtheSV40largeTantigenusingaretrovirus.Theestablishedcelllineswereconfirmedthroughmor-phologyobservation,immunnocytochemicalstainingandRT-PCRanalysis.TheHath1genewastransferredintothecelllinesusingadenovirus-mediatedtechniquestoexploretheirpotentialtodifferentiateintohaircells.ResultsTheestablishedcelllineswerestablymaintainedformorethan20passagesanddisplayedmanyfeaturessimilartoprimaryGERandLERcells.Theygrewinpatchesandassumedapolygonalmorphology.ImmunostainingshowedlabelingbySV40largeTantigenandIslet1(aspecificmarkerforGERandLER).AllpassagesofthecelllinesexpressedSV40largeTantigenonRT-PCRanalysis.Thecellsalsoshowedthecapabilitytodifferentiateintohaircell-likecellswhenforcedtoexpressHath1.ConclusionRetrovirus-mediatedgenetransfercanbeusedinestablishingimmortalizedprogenitorhaircelllinesinnewbornrat,whichmayprovideaninvaluablesystemforstudyinghaircelldifferentiationandregenerationfornewtreatmentofsensoryhearinglosscausedbyhaircellloss.

简介：微卫星标记484/485和484/W2R被用来在米饭细菌原生质在Wx地点识别复等位基因。十五等位基因被使用微卫星班和G-T多型性在278accessions识别。在这些等位基因之中，(CT)_(12)-G,(CT)_(15)-G,(CT)_(16)-G,(CT)_(17)-G,(CT)_(18)-G并且(CT)_(21)-G没被报导。在Wx地点带不同等位基因的72根单个片断的替换线(SSSL)被使用Huajingxian74与开发(CT)是的_(11)-G等位基因作为施主在Wx地点包含12不同等位基因的一个接受者和20就职。代替的片断的估计的长度与17.4厘米的一般水准从2.2～77.3厘米。

简介：TounderstandthewildOryzagenomeeffectonphotosynthesisanditsrelationtototaldrymatteraccumulationinanelitericevariety,asetof40stableintrogressionlines(ILs)BC3F8derivedfromacrossofOryzasativa(KMR3)×Oryzarufipogon(WR120)weregrownunderwellwateredconditions.Leafgasexchangemeasurementsandleafchlorophyllestimateswereconductedatthefloweringstage.Theresultsrevealedsignificantvariationsinnetphotosyntheticrate(Pn),transpirationrate(E),transpirationefficiency(Pn/E)andcarboxylationefficiency(Pn/Ci).PnshowedsignificantpositivecorrelationwithE,stomatalconductance(gs),Pn/Ciandtotalcanopydrymatter.SpecificleafareaandleafthicknesswerenotsignificantlycorrelatedwithPn.Thirty-sevenoutof40ILsshowedhigherPnthanKMR3[11.28μmol/(m2·s)],and20ILsshowedhigherPnthanWR120[15.08μmol/(m2·s)].ThelineIL194showedthehighestPn[21.62μmol/(m2·s)]withincreasedtotalcanopydrymatterfollowedbylinesIL381,IL106,IL363-12,IL198,IL86-18andIL50,whichexhibitedPnabove18.0μmol/(m2·s).TheILswithenhancedPnareapotentialsourcefordevelopingricevarietiesandhybridswithhigherbiomassandyield.

简介：Objective:Oxidativestressislinkedtoincreasedriskofgastriccancerandmatrixmetalloproteinases(MMPs)areimportantintheinvasionandmetastasisofgastriccancer.WeaimedtoanalyzetheeffectoftheaccumulationofoxidativestressinthegastriccancerMKN-45and23132/87cellsfollowinghydrogenperoxide(H2O2)exposureontheexpressionpatternsofMMP-1,MMP-3,MMP-7,MMP-9,MMP-10,MMP-11,MMP-12,MMP-14,MMP-15,MMP-17,MMP-23,MMP-28,andβ-cateningenes.Methods:ThemRNAtranscriptsinthecellsweredeterminedbyRT-PCR.FollowingH2O2exposure,oxidativestressintheviablecellswasanalyzedby2’,7’-dichlorofluoresceindiacetate(DCFH-DA).Caffeicacidphenethylester(CAPE)wasusedtoeliminateoxidativestressandtheconsequenceofH2O2exposureanditsremovalontheexpressionsofthegeneswereevaluatedbyquantitativereal-timePCR.Results:TheexpressionsofMMP-1,MMP-7,MMP-14,MMP-15,MMP-17andβ-catenininMKN-45cellsandonlytheexpressionofMMP-15in23132/87cellswereincreased.RemovaloftheoxidativestressresultedindecreaseintheexpressionsofMMPgenesofwhichtheexpressionswereincreasedafterH2O2exposure.β-catenin,atranscriptionfactorformanygenesincludingMMPs,alsodisplayeddecreasedlevelsofexpressioninbothofthecelllinesfollowingCAPEtreatment.Conclusions:OurdatasuggestthatthereisaremarkablelinkbetweentheaccumulationofoxidativestressandtheincreasedexpressionsofMMPgenesinthegastriccancercellsandMMPsshouldbeconsideredaspotentialtargetsoftherapyingastriccancersduetoitscontinuousexposuretooxidativestress.

简介：ThecrookedseismiclinesalongvalleyswereirregularpreviouslyinFuxianofShanbei,showinganirregularbranchinplane,andhardtocompleteclosegrids.Therefore,it'sdifficulttoconductreservoirinversionof2Dseismicdata.In2001,ZhongyuanOilfieldCompanycarriedoutthestudyonfieldacquisitionmethodsandseismicprocessingtechnologyinFuxian.Straightlineswerepassingthroughplateausandformedseismicgridsbyusingflexiblegeometrywithvariablelinearbins.Dataprocessinginvolvedmodel-inversionbasedrefractionstaticcorrection,surfaceconsistentamplitudecompensation,deconvolution,andpre-stacknoiseattenuation.Astheresult,seismicdatawithahighfidelitywasprovidedforthesubsequentreservoirpredictions,small-amplitudestructureinterpretationandintegrativegeologicstudy.Becausealllineswerejointedtoformgrids,comprehensiveinterpretationofreservoirinversioncouldbefinallyimplementedbyusingthepseudologgingmethodtocontrollineswithoutwells.

简介：Objective:Cancercellradioresistanceisastumblingblockinradiationtherapy.TheactivityinthenuclearfactorkappaB(NFκB)pathwaycorrelateswithanti-apoptoticmechanismsandincreasedradioresistance.TheIKKcomplexplaysamajorroleinNFκBactivationuponnumeroussignals.Inthisstudy,weexaminedtheinteractionbetweenionizingradiation(IR)anddifferentmembersoftheIKK-NFκBpathway,aswellasupstreamactivators,RAF1,ERK,andAKT1.Methods:Theeffectof4GyofIRontheexpressionoftheRAF1-ERK-IKK-NFκBpathwaywasexaminedinA549andH1299lungcancercelllinesusingWesternblotanalysisandconfocalmicroscopy.WeexaminedchangesinradiationsensitivityusinggenesilencingorpharmacologicalinhibitorsofERKandIKKβ.Results:IKKα,IKKγ,andIκBαincreaseduponexposuretoIR,therebyaffectingnuclearlevelsofNFκB(phospho-p65).ERKinhibitionorsiRNA-mediateddown-regulationofRAF1suppressedthepost-irradiationsurvivaloftheexaminedlungcancercelllines.AsimilareffectwasdetectedonsurvivaluponsilencingIKKα/IKKγorinhibitingIKKβ.Conclusions:ExposureoflungcancercellstoIRresultsinNFκBactivationviaIKK.ThegeneticorpharmacologicalblockageoftheRAF1-ERK-IKK-NFκBpathwaysensitizescellstotherapeuticdosesofradiation.Therefore,theIKKpathwayisapromisingtargetfortherapeuticinterventionincombinationwithradiotherapy.

简介：Thispaperpresentstheanalysisofopenmicrostripstructuresbyusingdiakopticmethodoflines(ML)combinedwithperiodicboundaryconditions(PBC).Theparametersofmicrostrippatchareobtainedfrompatchcurrentexcitedbyplanewave.ImpedancematrixelementsarecomputedbyusingfastFouriertransform(FFT),andreducedequationissolvedbyusingdiakoptictechnique.Consequently,thecomputingtimeisreducedsignificantly.TheconvergencepropertyofsimulatingopenstructurebyusingPBCandthecomparisonofthecomputertimebetweenusingPBCandusualabsorbingboundarycondition(ABC)showthevalidityofthemethodproposedinthispaper.Finally,theresonantfrequencyofamicrostrippatchiscomputed.Thenumericalresultsobtainedareingoodagreementwiththosepublished.

简介：DuringtwoyearsoddperiodfromDec.1987toFeb.1990,therehadbeenrepeatedlypollution-flashoverfailuresonour500kVtransmissionlines,resultinginawidescopeblackout.Therewereeleven500kVlinesaltogetherinNorth-east,EastandNorthChinapowergrids,wherepollution-flashoverfailureshappenedoneafteranotheron36transmissionlinetowers(poles).Since500kVtransmissionlinesarethemaintrunksofhighvoltage

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简介：Netemissioncoefficientsofradiationwerecalculatedforisothermalplasmaofmethaneasafunctionoftheplasmatemperature5,000～30,000Kandthearcradius0mmto10mmatatmosphericpressure.Calculationstakeintoaccountcontinuumandlineradiations,specialattentionhasalsobeengiventotheinfluenceofoverlappingspectrallines.ThelineshapesinourcalculationsaregivenbyconvolutionofDopplerandLorentzprofiles,resultinginasimplifiedVoigtprofile.Inthecaseofahydrogenprofile,weusedtheVidaltables,andthefourfirstLymanlinesandthefourfirstBalmerlineswereconsidered.Thiscalculationwascarriedoutontheassumptionoflocalthermodynamicequilibriumandinanondiffusiveenvironment.ThenetemissioncoefficientcalculationforpureargonplasmawascomparedwiththeexperimentalresultsofEvansinourpreviouswork.Weusedtheescapefactor,andourresultswerehigherthanthoseoftheexperiment.Inthispaper,weexplainwhy,usingtheescapefactor,theradiationisoverestimated.ThenetemissioncoefficientobtainedfromarealspectrumwascomparedwithEssoltaniwork’swhichusedtheline-by-linemethodforpureargonplasma.

简介：由202根线组成的一张回交重组体近交系人口从XieqingzaoB//XieqingzaoB/Dongxiang野米饭被开发。人口在植物hopper抵抗和收益特点上是assayedwithDNA标记和phenotyped。A连锁图在于of119脱氧核糖核酸标记并且跨越了因为在12个米饭染色体上的1188厘米被构造。Thirty-twochromosomal片断替换线在标记loci基于XieqingzaoB等位基因的百分比被选择。这些线具有为基因印射和相异的基因基因渗入的大潜力。

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简介：105根转基因的米饭线的一个总数独立地与豌豆含铁听基因(Fer)转变了以前被获得。在selfing和贝它-glucuronidase(GUS)的七代帮助了选择以后，有稳定的农学的特点的82根转基因的线被得到。在82根转基因的线，二高铁的转基因的米饭线Fer34和Fer65之中，与在milled的铁内容，是4.82和3.46野生型Xiushui11的预定，分别地被识别。在二根转基因的线，每基因外长高度被表示，并且继承为一个单个地点。transgene没在米饭植物的农学的特点上有否定效果，另外的矿物质营养的部件，外观质量和milled的吃的质量，显示这二根线是精英高铁的繁殖线。而且，实际申请和引起线的二精英的进一步的研究便于利用被讨论。

简介：Anewapproach,basedonthewaveformrelaxationtechniqueandfastWalshtrans-form,ispresentedtoanalyzethecoupledloosytransmissionlines(CLTL)witharbitraryterminalnetworks.Thesimulationaccuracyofthenewmethodcanbegreatlyimproved,thedisadvantagewhichalwaysexistsinpreviousmethodscanbeavoidedandaconsiderablesavingintimeandmemoryofCPUisobtained.

简介：-Thispapercarriesouttheanalysisofmechanicsofagripsystemofthree-key-boardhydraulictongsdevelopedforoffshoreoilpipelineswhichhasbeensuccessfullyusedinoilfieldsinChina.Themainimprovementofthissystemisthataleverframestructureisusedinthestructuraldesign,whichreducesgreatlythestressesofthemajorcomponentsoftheoilpipetongs.Theoreticalanalysisandnumericalcalcu-lationbasedonthirteenbasicequationsdevelopedshowthattheteethboardofthetongsisnoteasytoslipasfrequentlyhappenstoothersystemsandisofhigherreliability.

简介：在检验开拓殖民地于耳朵的害虫抵抗以后，从玉米(宝石)的USDA艺术Germplasm改进的20根玉米线编程序为喂锭盘的秋天粟蚕(FAW)(Spodopterafrugiperda)被评估用4玉米的抵抗是的生来的线抵抗、易受影响的控制。在锭盘舞台(V6-V8)的在在群袭以后的7-d和14-d的FAW损害级别，和食肉动物丰富和差异在2009和2010被记录。在每个试验性的阴谋的差异和许多食肉动物的调查被进行在FAW群袭以后的7d。检验的20根germplasm线，他们中的3个(即，条目9，15，和19那从热带玉米germplasm线被导出从乌拉圭，古巴，和泰国被发源，分别地)作为使用叶损害级别和食肉动物调查数据的最好的FAW抵抗的germplasm线被识别。另外，食肉动物的丰富和差异比在2009在2010是更大的，它可能在在2010检验的所有线上引起了FAW损害级别的底层。2年的数据证明FAW损害级别否定地被相关到食肉动物丰富和差异，它也是由genotype×的影响环境相互作用。调查结果建议热带germplasm是到FAW和玉米一种有害幼虫的本国的抵抗的重要来源。同时，玉米genotype×环境相互作用(例如，食肉动物优美，和变化天气条件)应该在地条件下面在昆虫的多重年的评估和玉米germplasm线的疾病抵抗被包括。

简介：Roll-leaf-1(rl-1)andspot-leaf-1(spl-1)weretwonear-isogeniclines,whichwereobtainedafter3to4backcrosseswithearlyseasonindicariceZhefu802asrecurrentparent.Hennamacro-lesions,referredasphysiologicalormorphologicalmarkers,begantoappearonleavesat4.5-to6.0-leafstage.Thericeseedlingswereinoculatedat3.5-,5.0-and7.0-leafstageswithhighpathogenicracesZhongA1andZhongB1ofMagnaporthegrisea,respectively.Theresistanceofrl-1,spl-1andZhefu802againstblastwassignificantlydifferent.TheseedlingsofZhefu802at3.5-to7.0-leafstageweresusceptibletoracesZhongA1andZhongB1ofM.grisea,whereasthoseofrl-1andspl-1at3.5-,5.0-and7.0-leafstagesweresusceptible,moderatelyresistantandresistant,respectively.Theseresultssuggestedthattheenhancedresistanceofri-1andspl-1relatedtotheappearanceoftheirmorphologicalmarkerlesions.Theexperimentprovidedabasisforstudyinglesionmimicandhypersensitiveresponseinassociationwithdiseaseresistance.