简介：AbstractObjective:Second-generation antipsychotics are widely used in mental illness, but the treatment effects and side effects are affected by single nucleotide polymorphisms (SNPs) of related genes. Quetiapine and aripiprazole are two frequently used secondgeneration antipsychotic drugs. The aim of this study was to develop two different SNP detection methods for four SNP alleles associated with the pharmacokinetics of quetiapine and aripiprazole, based on high-resolution melting (HRM) and multicolor melting curve assay (MMCA) respectively.Methods:Whole genome DNA samples were obtained from 240 healthy people (107 females and 133 males) without genetic diseases. HRM methods were established using four kinds of specific primers and a saturated fluorescent dye. Each SNP allele with their own primers was detected in a single reaction. In the MMCA method, a multiplex polymerase chain reaction with 4 different-colored fluorescent probes was established to detect four SNP alleles in a single reaction. All experimental protocols were approved by the Ethics Committee of the Shanghai Children’s Medical Center, China (SCMC-201015) on November 22, 2010.Results:Two detection methods for the pharmacogenomics of quetiapine and aripiprazole, based on HRM and MMCA respectively, were established in this study. The single-target HRM method can be completed in 96 minutes, whereas the quadruplex MMCA method takes 133 minutes. It was found that the results of HRM and MMCA for the four SNP alleles had 100% coincidence with Sanger sequencing in the 240 samples.Conclusion:This study developed two methods for the detection of four pharmacogenomic SNP alleles that correlated with quetiapine and aripiprazole. Both methods are rapid, cost-saving, highly accurate and potentially facilitate rational use of second-generation antipsychotics for clinical medication.

简介：ImagegeometrydistortionisanimportanttechnicalstandardinB-modeultra-sounddiagnosissystem.ThispaperinvestigatesthecauseofimagedistortioninphasedarrayB-modeultrasounddiagnosissystem.Thefirstdistortioncauseisthedelayerrorofcompoundwavebind.Phasedarrayultrasoundtranduceriscomposedofmanyrectangleunits.Withcontrollinginspiringplustime,ultrasoundwave

简介：AbstractAlbumin solutions derived from human plasma have demonstrated clinical benefits as intravenous fluid therapy in clinical settings such as liver disease, sepsis, intensive care, and surgery. For all plasma-derived medicinal products, there is a potential risk from pathogens, including relevant blood-borne viruses, emerging viruses, and prion proteins. To minimize the risk of transmissible infections, the production of human albumin solutions includes rigorous donor selection and plasma testing, and effective pathogen removal and inactivation methods such as fractionation and pasteurization. Compliance with international pharmacopeial standards for purity and prekallikrein activator and aluminum content is crucial, as is post-marketing pharmacovigilance for the continuous monitoring of adverse events. This review focuses on the effectiveness of manufacturing methods in the production of plasma-derived albumin, to ensure the safety of hyperoncotic solutions for volume expansion. We evaluated evidence identified through online database (PubMed) searches and from unpublished sources, on the manufacturing and pathogen safety of plasma-derived albumin solutions. The results confirmed the already established and evolving pathogen reduction capacity of the reviewed manufacturing methods. Up-to-date post-marketing pharmacovigilance data and log10 reduction factors for known and emerging pathogens during albumin production are included. Towards the goal of ever-increasing clinical safety, potential areas of improvement, such as compliance rates for the completion of donor health questionnaires, are also discussed. Taken together, the current manufacturing and pathogen reduction steps result in albumin products of greater purity than previous-generation products, with a high margin of pathogen safety against known and emerging pathogens, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

简介：Objective:Europeanlungcancerscreeningstudiesusingcomputedtomography(CT)haveshownthatamanagementprotocolbasedonmeasuringlungnodulevolumeandvolumedoublingtime(VDT)ismorespecificforearlylungcancerdetectionthanadiameter-basedprotocol.However,whetherthisalsoappliestoaChinesepopulationisunclear.Theaimofthisstudyistocomparethediagnosticperformanceofavolume-basedprotocolwithadiameter-basedprotocolforlungcancerdetectionandoptimizethenodulemanagementcriteriaforaChinesepopulation.Methods:Thisstudyhasapopulation-based,prospectivecohortdesignandincludes4000participantsfromtheHexidistrictofTianjin,China.Participantswillundergolow-dosechestCTatbaselineandafter1year.Initially,detectedlungnoduleswillbeevaluatedfordiameterandmanagedaccordingtoaroutinediameter-basedprotocol(ClinicalPracticeGuidelineinOncologyforLungCancerScreening,Version2.2018).Subsequently,lungnoduleswillbeevaluatedforvolumeandmanagementwillbesimulatedaccordingtoavolume-basedprotocolandVDT(aEuropeanlungnodulemanagementprotocol).Participantswillbefollowedupfor4yearstoevaluatelungcancerincidenceandmortality.TheprimaryoutcomeisthediagnosticperformanceoftheEuropeanvolume-basedprotocolcomparedtodiameter-basedmanagementregardinglungnodulesdetectedusinglow-doseCT.Results:Thediagnosticperformanceofvolume-anddiameter-basedmanagementforlungnodulesinaChinesepopulationwillbeestimatedandcompared.Conclusions:Throughthestudy,weexpecttoimprovethemanagementoflungnodulesandearlydetectionoflungcancerinChinesepopulations.

简介：[摘要] 目的：探讨儿科临床实习教学方法。方法：从2019年1月至2020年12月，我院儿科接收的52名实习生为对象。采用随机数表法划分为对照组（采用传统教学方法）、试验组（采用PBL教学法）两个组别，均为26名。比较实习考核成绩和教学满意度。结果：教学后两组实习生的考核成绩均提高，且试验组成绩优于对照组，对教学过程的满意率高于对照组（96.15%vs76.92%），有统计学意义（P＜0.05）。结论：对于儿科临床实习生，采用PBL教学法优于传统教学方法，能提高教学满意度和考核成绩。

简介：[摘要] 目的：探讨血透新护士的带教方法。方法：从2019年1月至2020年12月，我院血透室接收培训的44名新护士为对象。采用随机数表法划分为对照组（采用传统教学模式）、试验组（采用一对一带教模式）两个组别，均为22名。比较考核成绩和教学满意度。结果：试验组护士在理论知识和临床操作上的考核得分均高于对照组，教学满意率（100.00%）比对照组（81.82%）要高，有统计学意义（P＜0.05）。结论：对血透新护士采用一对一带教模式，能提高教学效果和护士的满意度。

简介：Diastemaoftenoccuredinmaxillarycompletedentureresinbaseintheclinic,although"Lucitone199"hadbeenusedwithmoretensilestrength.Mechanicalex-perimentshaveprovedthehightransversetensilestress(σ_x~+)concentratinginanteri-orteethregionandtheirpalatalsurfaceofmaxillarycompletedentureresinbasewhileitwasexposedtoverticalloadingincenterocclusion.Settingstrengtheningnetinresinbasehasachievedbetterresults.Forevaluatingpurpose,twentykinds

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简介：根据古老、现在的中国来源的意见，针灸和罐头的八汇合的Pointsare必需品治疗各种疾病。他们对12个常规顶点施加基本影响，他们的动员能为一个成功的针灸处理被看作必需品。为这，他们应该在针灸起一个主要作用。在Europeanacupuncture教训，也有意见，由太多刺破有机体的qi可能得到的八ConfluentPoints用尽了哪个将败坏病人“s症状并且将最后创造新症状。这里，恶化以及新症状的创造被不在如果症候群区别，点选择和刺激是正确的，在古老的中国建立了的针灸观察禁止徵候由于针灸引起，这将被显示出。在到这的反对，所有八条非凡的隧道将起初被激活然后12个常规顶点将被动员以便完成治疗学的效果。