简介：BackgroundAlthoughmiRNAshavebeenshowntoassociatewithavarietyofdiseases,whethermiRNAsinmonocyteassociateheartfailure(HF)hasbeennotwellstudied.MethodsEightpatientswithischemicHF(IHF),8patientswithnon-ischemicHF(NIHF)and8healthyvolunteerswererecruited.Clinicalcharacteristicsofallparticipantswerecollected.PeripheralbloodsamplesweredrawnforanalysisofmiRNAexpressioninmonocytes.ResultsAllparticipantsweremaleandtheparticipantsinIHFgroupwereolderandhadhigherpercentageofsmokeranddiabetesmellitusthanintheothertwogroups(P<0.05).SerumlevelsofcreatinineandNT-proBNPweresignificantlyhigherinIHFpatientscomparedtotheothertwogroups(P<0.05).MoreparticipantsinIHFgroupweretreatedwithACEI/ARB,beta-blockerandstatins.ParticipantswithNYHAgradeIIIaccountedfor62.5%inIHFgroup,whileparticipantswithNYHAgradeIVaccountedfor87.5%inNIHFgroup.Thelevelsof11miRNAsinmonocytesweresignificantlyhigherintheIHFgroup,andthelevelsof7miRNAsweresignificantlyincreasedintheNIHFgroup.OtherdifferencesinmiRNAslevelsbetweenIHFandNIHFgroupswerealsoobserved.ConclusionourpresentstudyrevealedthattherearesubstantialdifferencesinmiR-NAsbetweenHFpatientsandhealthyvolunteer.

简介：BackgroundStudieshaveshownthatmiRNA-155playedanimportantroleintheprocessofdevelopmentofhypertension.However,thereisnodateaboutmiRNA-155andbloodpressuremonitoringparameters.Therefore,weexaminedwhetherinhypertensivepatientstheexpressionlevelofplasmamiRNA-155relatedto24-hambulatorybloodpressuremonitoring(ABPM)parameters.MethodsAcohortofadultpatientsscheduledtoreceivephysicalexamination,officeandambulatorybloodpressuremonitoring.Quantitativereversetranscriptasepolymerasechainreaction(qRT-PCR)wasusedtoevaluatetheexpressionofselectedmiRNA-155.ThemiRNA-155expressionlevelcorrelationbetweenbloodpressureparameterswasassessedusingtheSpearmancorrelationcoefficient.ResultsFiftyfouressentialhypertensionpatients(25men;meanage,53.28±9.52years)andthirtyhealthyvolunteers(15men;meanage,53.03±5.87years)wereincluded.WeobservedhigherexpressionlevelofmiRNA-155(32.31±2.85vs27.21±1.59,P<0.001)inhypertensivepatientscomparedtohealthycontrolindividuals.MiRNA-155expressionlevelshowedsignificantpositivecorrelationwith24hDaytimeSBP(r=0.681,P<0.001),24hDaytimeDBP(r=0.473,P<0.001),24hDaytimePP(r=0.565,P<0.001)anddipping(r=0.257,P=0.018),respectively.ConclusionsOurstudyshowedthatmiRNA-155expressionlevelwasassociatedpositivelywithdaytimebloodpressuremonitoringparameters,aswellasbloodpressurevariability,indicatingapossibleimplicationofmiRNA-155inthepathogenesisofhypertension.

简介：扩张型心肌病（DCM）是非缺血性心肌病中最普遍的类型,预后差,死亡率高。近年来的研究发现,非编码小分子RNA（miRNA）与DCM的病程密切相关。本文就miRNA的表达模式与DCM发展的相关研究进行综述。

简介：目的探讨X综合征患者QT离散度(QTd)改变意义.方法对21例(A组)X综合征患者QTd与20例(B组)正常健康人进行对比.结果A组QTmax、QTmax与B组比较差异有显著性(P＜0.05),而QTd、QTcd无明显差异(P＞0.05).结论X综合征有慢性交感神经系统张力增高,临床选用肾上腺素能受体阻滞剂治疗有一定帮助.

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简介：BackgroundTheremaybedysregulationofcirculatingmicroRNAsinacutemyocardialinfarction(AMI),whichisanaging-relatedprocess.However,thedifferencebetweenyoungandelderlypeopleinexpressionlevelofcirculatingmiR-21inAMIpatientshasnotbeeninvestigated.MethodsThestudyincluded72consecutivepatientswithAMI.ThegroupIconsistedof43patientsagedequaltoorabove65yearsandthegroupIIconsistedof29patientsagedequaltoorbelow45years.Real-timeRT-PCRwasappliedtodetectserummiR-21expressionlevelsatthetimeofmechanicalreperfusionand12h,D1,D3andD7afterPCI,respectively.ResultsTheexpressionlevelofmiR-21inAMIpatientsincreasedmarkedly12hafterPCIandreachedthepeakatD1afterPCIinbothgroups.TherewasnodifferenceofmiR-21expressionbetweenGroupⅠandⅡatthetimeofmechanicalreperfusion(5.12±0.73vs.4.98±0.87)andD7afterPCI(1.28±0.75vs.1.94±0.89),However,groupⅠpatientsexhibitedhighermiR-21expressionlevelthangroupⅡat12h(7.96±0.78vs.4.23±0.77,P<0.05),D1(9.32±0.89vs.6.12±0.92,P<0.05)andD3(4.78±0.91vs.2.97±0.77,P<0.05)afterPCI,respectively.ConclusionOurdatarevealanincreaseofmiR-21inpatientswithAMImaybeamechanismofmyocardialischemiareperfusioninjury.TheexpressionofmiR-21wasrelatedtothedevelopmentandprogressionofAMI,andthereisanage-relatedchangeintheexpressionofmiR-21inacutemyocardialinfarctionpatients.

简介：目的探讨中心分流术在治疗重症法洛四联症(tetralogyofFallot,TOF)中的应用,评价其手术效果.方法回顾性分析21例应用Gore-Tex管(膨体聚四氟乙烯人工血管)行升主动脉到主肺动脉中心分流手术的TOF患者的临床资料.着重分析手术方法、手术结果及术后随访.结果手术死亡2例,术后3d内分别死于低心排血量综合征和肺水肿,病死率9.5%(2/21).12例于术后6个月～24个月内行二期手术根治,无死亡患者.根治术中无发现Gore-Tex管堵塞者.术后动脉血氧饱和度及动脉血氧分压比术前显著改善[90.0%±4.0%比60.0%±5.05%,P<0.01;(53.4±14.1)mmHg比(42.2±8.8)mmHg,P<0.01].12例患者二期根治术时与中心分流术时比较,左心室及肺动脉发育均明显改善.结论中心分流术是改善重症法洛四联症症状、体征可供选择的手术方式,是重症法洛四联症根治术的一种良好的分期手术方式.

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简介：BackgroundH9c2celllineismononucleatedmyoblastderivedfromembryonicrathearttissue.ActivitiesofTGF-β1,MMP-2andMMP-9increaseinH9c2cellsaftertreatmentwithfibrosisstimuli.MicroRNA(miRNA),akindofendogenoussmallnon-codingRNA,participatesincardiacfibrosis.Inthepresentstudy,expressionsoffibrosis-relatedgenesandmicroRNAsinTGF-β1treatedH9c2cellswereinvestigated.MethodsExpressionsoffibrosis-associatedgenes,includingCol3a1,α-SMA,FN1,CTGFandTSP-1,weremeasuredinTGF-β1treatedH9c2cellsbyquantitativereversetranscriptionandPCR(qRTPCR).Levelofα-SMAinH9c2cellswasdemonstratedbyfluorescenceimmunohistochemistry(FIHC)assay.ExpressionsofmaturemiR-16,-21a,-29binH9c2cellsweredeterminedbyqRT-PCRassay.ActivationsofSmad3andNF-kBsignalinginTGF-β1-treatedH9c2cellswerestudiedbydualluciferaseassay.ExpressionsofCol3a1,α-SMA,FN1,CTGFandTSP-1weredetectedinH9c2cellswithadenovirus-mediatedoverexpressionofmiR-21a.ResultsqRT-PCRassayshowedthatα-SMA,FN1,CTGF,TSP-1,butnotCol3a1,wereup-regulatedinTGF-β1treatedH9c2cells.FIHCresultalsorevealedthatα-SMAwasincreasedinTGF-β1-treatedH9c2cells.Consistently,dualluciferaseassayshowedthatSmad3andNF-kBsignalingproteinswereactivatedinTGF-β1-treatedH9c2cells.miR-21a,butnotmiR-16and-29b,wassignificantlyup-regulated.Additionally,over-expressionofmiR-21asignificantlyincreasesmRNAexpressionsofα-SMA,FN1,CTGFandTSP-1inH9c2cells.ConclusionsMiR-21aisup-regulatedinTGF-β1treatedH9c2cells,andmaycontributetoup-regulationsoffibrosis-associatedgenes.