简介:Inathree-dimensionaloff-latticemodel,themethodofShakhnovichandGutinforminimizingtheHamiltonianisappliedtothedesignofaprotein-modelchain.Theeffectofthenumberofhydrophobicandhydrophilicmonomertypesonthedesignabilityofaprotein-modelchainisinvestigated.Thesimulationresultsrevealthatthenumberofhydrophobicmonomertypesisamuchmoreimportantfactorthanthatofthepolarmonomertypesinthedesignofaprotein-modelchain.
简介:假设在在分子的水平的生活进程的主要变量是生物大分子和他们的边疆电子的符合构造符合构造电子系统上的量子论的一个形式体系被建议。基于符合构造电子系统的量子论,当在扭转之间的量转变在多肽上声明链,和合拢的率由nonadiabatic是计算的,合拢的蛋白质被考虑操作员方法。率计算在合拢被概括到频率变化的盒子。合拢率公式的蛋白质的一种分析形式被获得,它能为蛋白质合拢的进一步的学习作为一个有用工具被服务。率理论的申请简短被总结解释合拢实验的蛋白质。它包括合拢率的惯性的时刻依赖,对的统一描述二状态并且合拢的multistate蛋白质,合拢并且展开率对变性剂集中的关系,在放出能、吸能的合拢之间的区别,ultrafast并且下坡的合拢从量合拢看了理论,并且,最后,合拢率和它的non-Arrhenius行为的解释的温度依赖。所有这些研究支持合拢的蛋白质是的看法实质上,在conformational之间的量转变说。
简介:Proteomicsisthestudyofproteinsandtheirinteractionsinacell.WiththesuccessfulcompletionoftheHumanGenomeProject,itcomesthepostgenomeerawhentheproteomicstechnologyisemerging.Thispaperstudiesproteinmoleculefromthealgebraicpointofview.Thealgebraicsystem(Σ,+,)isintroduced,whereΣisthesetof64codons.Accordingtothecharacteristicsof(Σ,+,),anovelquasi-aminoacidscodeclassificationmethodisintroducedandthecorrespondingalgebraicoperationtableoverthesetZUofthe16kindsofquasi-aminoacidsisestablished.Theinternalrelationisrevealedaboutquasi-aminoacids.Theresultsshowthatthereexistsomeveryclosecorrelationsbetweenthepropertiesofthequasi-aminoacidsandthecodon.Allthesecorrelationrelationshipsmayplayanimportantpartinestablishingthelogicrelationshipbetweencodonsandthequasi-aminoacidsduringthecourseoflifeorigination.AccordingtoMaFetal(2003J.AnhuiAgriculturalUniversity30439),thecorrespondingrelationandtheexcellentpropertiesaboutaminoacidscodeareverydifficulttoobserve.Thepresentpapershowsthat(ZU,⊕,)isafield.Furthermore,theoperationalresultsdisplaythatthecodontgahasdifferentpropertyfromotherstopcodons.Infact,inthemitochondrionfromhumanandoxgenomiccodon,tgaisjusttryptophane,isnotthestopcodonlikeinothergeneticcode,itisthecaseoftheChenWCetal(2002ActaBiophysicaSinica18(1)87).Thepresenttheoryavoidssomeinexplicableeventsofthe20kindsofaminoacidscode,inotherwordsitsolvestheproblemof'the64codonassignmentsofmRNAtoaminoacidsisprobablycompletelywrong'proposedbyYang(2006ProgressinModernBiomedicine63).
简介:Direct-methodphaseextensionhasbeenappliedtotwo-dimensionalelectrondiffractiondataoftheproteinstreptavidin.Structure-factoramplitudesfromelectrondiffractionwerecombinedwithphasesfromthecorrespondingelectronmicrographs.Maximum-entropydiscriminationandclusteranalysiswereusedtoderiveasolutionfromalargenumberofrandomtrials.Thephaseextensionfrom0.3to0.25nmledtosubstantialimprovementofthereconstructedprojectionimagequality.
简介:BecauseERstressisoneofthesignalingpathwaysinvolvedintheregulationofautophagy,wehypothesizedthatERstressmightalsoplayanimportantroleinradiation-inducedautophagy.SHG44andHeLacellswereirradiatedwithX-raysandcarbonionsof30and75keV/mat2Gy.TheexpressionlevelsofBip,amajorindicatorofUPR,at4and24hpost-irradiationareshowninFig.1(a)and(b)forSHG44andHeLacells,respectively.