简介：

简介：Theadsorptionofproteinonnanoparticleswasstudiedbyusingdynamiclightscatteringtomeasurethehydrodynamicsizeofbothpureproteinandnanoparticlesadsorbedwithdifferentamountsofprotein.Thethicknessoftheadsorbedproteinlayerincreasesasproteinconcentration,butdecreasesastheinitialsizeofnanoparticles.Afterproperlyscalingthethicknesswiththeinitialdiameter,weareabletofitallexperimentaldatawithasinglemastercurve.Ourexperimentalresultssuggestthattheadsorbedproteinsformamonolayeronthenanoparticlesurfaceandtheadsorbedproteinmoleculesareattachedtotheparticlesurfaceatmanypointsthroughapossiblehydrogen-bonding.Ourresultsalsoindicatethatasproteinconcentrationincreases,theoverallshapeoftheadsorbedproteinmoleculecontinuouslychangesfromaflatlayerontheparticlesurfacetoastretchedcoilextendedintowater.Duringthechange,thehydrodynamicvolumeoftheadsorbedproteinincreaseslinearlywithproteinconcentration.

简介：AstatisticalthermodynamictheoryoflinearproteinsolutionswasproposedwiththeaidofalatticemodelandappliedtotypeⅠantifreezeprotein(AFPI)solutions.ThenumericalresultsforseveralAFPIsolutionsshowthattheGibbsfunctionofthesolutionhasaminimumatacertainproteinconcentration,buttheproteinchemicalpotentialincreaseswithincreasingtheconcentration.TheinfluencesoftemperatureandproteinchainlengthontheAFPIchemicalpotentialwerealsodiscussed.Theevaluationforthecolligativedepressionofthefreezingpointconfirmsthattheantifreezeactionshouldberecognizedasnon-colligative.Thetheoreticaldeductionfortheconcentrationdependenceofthethermalhysteresisactivitycoincidesqualitativelywiththepreviousexperimentalandtheoreticalresults.

简介：Azurins,awildtypeandageneticallymutantK27alteredone,wereimmobilizedonannealedgoldsurfaceandinvestigatedbymeansofatomicforcemicroscopy.Itwasfoundthatthesurfacecoverageandheightdistributionoftheadsorbedproteinmoleculesaredifferentfromeachother,whichispossiblytheresultofthedifferentorientationonthesurface.Itisbelievedthatthewildtypeazurinisconnectedtogoldsurfacebythedisulphidebridge;whilethemutant,K27C,mightbethroughthethiolgroupsofthecysteineresiduesontheirsurface.

简介：正弦电场驾驶的一个蛋白质马达系统被调查。Thesignal-to-noise比率(SNR)在断热的限制被导出。随机的回声的现象被作出对有利的裁决这个蛋白质马达系统。

简介：Inathree-dimensionaloff-latticemodel,themethodofShakhnovichandGutinforminimizingtheHamiltonianisappliedtothedesignofaprotein-modelchain.Theeffectofthenumberofhydrophobicandhydrophilicmonomertypesonthedesignabilityofaprotein-modelchainisinvestigated.Thesimulationresultsrevealthatthenumberofhydrophobicmonomertypesisamuchmoreimportantfactorthanthatofthepolarmonomertypesinthedesignofaprotein-modelchain.

简介：Inthisarticle,asphericalchitosangelcrosslinkedbyepichlorohydrinwasprepared.Itwasthenloadedwithcopperionstoproduceametalchelateaffinityadsorbentforprotein.Theuptakeofbovineserumalbumin(BSA)bytheaffinityadsorbentwasinvestigated,andtheadsorptioncapacityforBSAashighas40mg/g-wetbeadswasobserved.TheadsorptionequilibriumdatawaswellcorrelatedbytheLangmuirequation.TheadsorptionwasconsiderablyaffectedbypH.Inaddition,theamountofBSAadsorbedontothebeadsdecreasedwiththeincreasingofaqueousphaseionicstrength,soadsorbedBSAcanbedesorbedbyadjustingpHorionicstrengthofthesolution.

简介：假设在在分子的水平的生活进程的主要变量是生物大分子和他们的边疆电子的符合构造符合构造电子系统上的量子论的一个形式体系被建议。基于符合构造电子系统的量子论，当在扭转之间的量转变在多肽上声明链，和合拢的率由nonadiabatic是计算的，合拢的蛋白质被考虑操作员方法。率计算在合拢被概括到频率变化的盒子。合拢率公式的蛋白质的一种分析形式被获得，它能为蛋白质合拢的进一步的学习作为一个有用工具被服务。率理论的申请简短被总结解释合拢实验的蛋白质。它包括合拢率的惯性的时刻依赖，对的统一描述二状态并且合拢的multistate蛋白质，合拢并且展开率对变性剂集中的关系，在放出能、吸能的合拢之间的区别，ultrafast并且下坡的合拢从量合拢看了理论，并且，最后，合拢率和它的non-Arrhenius行为的解释的温度依赖。所有这些研究支持合拢的蛋白质是的看法实质上，在conformational之间的量转变说。

简介：Thispresentstudyinvestigatedtheabilityofvarioussoyproteinhydrolysates(SPHs)inbindingcalcium.ItwasdemonstratedthattheamountofCa-bounddependedgreatlyontheSPHsobtainedusingdifferentproteases,whichincluded:neutrase,flavourzyme,proteaseMandpepsin.ThemaximumlevelofCa-bound(66.9mg/g)occurredwhenproteaseMwasusedtohydrolyzesoyprotein.PeptidefragmentsexhibitinghighCa-bindingcapacityhadmolecularweightsofeither14.4or8-9kDa.ThelevelofCa-boundincreasedlinearlywiththeincrementofcarboxylcontentinSPHs,andfurtherdeamidationonSPHsfromproteaseMimprovedCa-bindingofthehydrolysate.

简介：Theinteractionofbromothymolblue(BB)withhumanserumalbumin(HSA)wasstudiedbyelectrochemicaltechniquesandasensitivemethodforproteinsassaywasdeveloped.WhenBBinteractedwithHSA,thevoltammetricpeakcurrentvalueofBBdecreasedlinearlywiththeconcentrationofHSAinarangeof1.0―40.0mg/L,andthepeakpotentialshiftednegatively.Basedontheresults,asensitiveassaymethodforproteins,suchasHSA,bovineserumalbumin(BSA),andeggalbuminetc.wasestablished.ThismethodwasfurtherappliedtodeterminingtheHSAinhealthyhumanbloodsamples,andtheresultsarenotsignificantlydifferentfromthoseobtainedbytheclassicCoomassieBrilliantBlueG-250spectrophotometicmethod.Thedetectingconditionsofthismethodwereoptimizedandtheinteractionmechanismwasdiscussed.Theresultsshowthattheelectrochemicalparameters(formalpotentialE0,standardrateconstantoftheelectrodereactionks,parameterofkineticnα)ofBBhavenoobviouschangesbeforeandaftertheinteraction,whichindicatethatBBcaninteractwithHSA,forminganelectrochemicalnon-activecomplex.Theequilibriumconstant(βs)andthebindingratio(m)forthiscomplexwerecalculated.Themis4andβsis1.41×1019.Thismethodisfast,simple,highlysensitive,andhasgoodselectivity,whichcanbeusedinclinicalmeasurements.

简介：Sumoylationisanimportantproteinmodificationdiscoveredrecently.SUMO(smallubiquitin-relatedmodifier)pathwayregulatestheproteinstabilityandtranscriptionalactivitywitha12-kDasmallmolecularprotein,SUMO,ligatedtothetargetprotein.ThepurificationofSUMOproteinsisakeysteptorevealtheirfunction.ThepurposeofthisstudywastoconstructtherecombinantSUMO1geneclonedtoapGEX-4T-1vectortoexpressandpurifytheSUMO1-GSTfusionproteininEscherichiacoli.First,thefulllengthDNAsequenceofSUMO1genewasamplifiedbyPCRandwasligatedtopMD18-Tvector.ThentheSUMO1genewassubclonedtopGEX-4T-1prokaryoticexpressionvectorbetweenBamHIandXhoIsites,andtransformedinEscherichiacoliDH5αcells.Therightcolonieswereidentifiedbyrestrictiveenzymedigestionandsequencing.ThecorrectrebombinantplasmidofpGEX-4T-1-SUMO1wastransformedinEscherichiacoliBL21cellsandtheninducedbyIPTG(isopropyl-β-D-1-thiogalacto-pyranoside)toexpresstheSUMO1-GSTfusionprotein.ThehighlypurifiedSUMO1-GST(glutathioneS-transferase)fusionproteinwasobtainedbyaffinitychromatography.Finally,thepropertiesofSUMO1-GSTfusionproteinwereconfirmedbyCoomassiebrilliantbluestrainandWesternblotanalysis.TherecombinantplasmidofpGEX-4T-1-SUMO1wassuccessfullyconstructed,andSUMO1-GSTfusionproteinsweresuccessfullyexpressed.

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简介：Preparationandcharacterizationofthehapten-proteinconjugatesarefundamentaltodevelopingenvironmentalimmunoassays.Asahapten,1-pyrenebutyricacid(PBA)wasconjugatedtothecarrierproteinofbovineserumalbumin(BSA)orovalbumin(OVA)byactiveestermethod.Infraredspectra(IR)showedthatPBA-BSAandPBA-OVAconjugatesweresuccessfullyprepared.Thenumberofthehaptensconjugatedtothecarrierproteinwasdeterminedbyultravioletspectra(UV)andmatrix-assistedlaserdesorptionionizationtime-of-flightmassspectrometry(MALDI-TOF-MS).ThecalculatedaveragebindingratiosofPBA/BSAandPBA/OVAwere18:1and10:1byUV,and31:1and22:1byMALDI-TOF-MS,respectively.Althoughtherewasadiscrepancybetweentheresultsdeterminedbythetwomethods,bothofthemwereusefulforthecharacterizationofthehapten-proteinconjugates.TheantibodywasproducedagainsttheantigenofPBA-BSA,andtheaffinitywastestedbythedoubleagardiffusionmethod.Theconjugatesandtheantibodycouldbeusedfordevelopingasensitiveandselectiveimmunoassayofpolycyclicaromatichydrocarbons(PAHs).

简介：

简介：Proteomicsisthestudyofproteinsandtheirinteractionsinacell.WiththesuccessfulcompletionoftheHumanGenomeProject,itcomesthepostgenomeerawhentheproteomicstechnologyisemerging.Thispaperstudiesproteinmoleculefromthealgebraicpointofview.Thealgebraicsystem(Σ,+,)isintroduced,whereΣisthesetof64codons.Accordingtothecharacteristicsof(Σ,+,),anovelquasi-aminoacidscodeclassificationmethodisintroducedandthecorrespondingalgebraicoperationtableoverthesetZUofthe16kindsofquasi-aminoacidsisestablished.Theinternalrelationisrevealedaboutquasi-aminoacids.Theresultsshowthatthereexistsomeveryclosecorrelationsbetweenthepropertiesofthequasi-aminoacidsandthecodon.Allthesecorrelationrelationshipsmayplayanimportantpartinestablishingthelogicrelationshipbetweencodonsandthequasi-aminoacidsduringthecourseoflifeorigination.AccordingtoMaFetal(2003J.AnhuiAgriculturalUniversity30439),thecorrespondingrelationandtheexcellentpropertiesaboutaminoacidscodeareverydifficulttoobserve.Thepresentpapershowsthat(ZU,⊕,)isafield.Furthermore,theoperationalresultsdisplaythatthecodontgahasdifferentpropertyfromotherstopcodons.Infact,inthemitochondrionfromhumanandoxgenomiccodon,tgaisjusttryptophane,isnotthestopcodonlikeinothergeneticcode,itisthecaseoftheChenWCetal(2002ActaBiophysicaSinica18(1)87).Thepresenttheoryavoidssomeinexplicableeventsofthe20kindsofaminoacidscode,inotherwordsitsolvestheproblemof'the64codonassignmentsofmRNAtoaminoacidsisprobablycompletelywrong'proposedbyYang(2006ProgressinModernBiomedicine63).

简介：Thehydrophilicityofsiliconehydrogelsusedassoftcornealcontactlensplaysanimportantroleinwearingcomfort.Inordertoenhancehydrophilicityandproteinresistance,siliconehydrogelmembranesweremodifiedbyatmosphericpressureglowdischargeplasma(APGDP)inducedsurfacegraftpolymerizationofN-vinylpyrrolidone(NVP)andpoly(oligoethyleneglycolmethylethermethacrylate)(PEGMA)inthispaper.XPSanalysisdemonstratedthesuccessofgraftpolymerizationofNVPandPEGMAontothesurfaceofsiliconehydrogelmembranes.Thehydrophilicityofsiliconehydrogelswascharacterizedbythemeasurementofwatercontactangle(WCA).TheresultshowedthatNVPgraftedsiliconehydrogelhastheWCAofabout68°andPEGMAgraftedsiliconehydrogelhasthelowestWCAofabout62°,whilethepristinesiliconehydrogelishydrophobicwiththeWCAofabout103°.Proteinresistanceofsiliconehydrogelswasinvestigatedbythemethodofbicinchoninicacidassayusingbovineserumalbumin(BSA)asamodel.It'sfoundthatthegraftedsiliconehydrogelhasasignificantimprovementofproteinresistance,andPEGMAgraftingismoreefficientforthereductionofproteinadsorptionthanNVPgrafting.ThesiliconehydrogelmembranesgraftedwithNVPandPEGMAaregoodcandidatesofsoftcornealcontactlenses.

简介：

简介：新pH应答的糖类hydrogels被设计并且准备了使用curdlan衍生物(curdlan-Boc-histidine，杂种狗--他的)。杂种狗--他的hydrogels拥有了高度多孔的结构。杂种狗的胀大的比率--他与Boc-histidine的替换的度增加的hydrogels组织。并且0.5mol/LNaCl的增加挑起了杂种狗的胀大的比率的锋利的减小--他的hydrogels。牛的浆液白朊(BSA)能高效地被包含进杂种狗--他的hydrogels。而且，在从杂种狗的不同pH价值的BSA的版本侧面--他的hydrogels是显著地不同的。这些hydrogels在cytotoxicity试金显示出好biocompatibility。杂种狗--他的hydrogels具有在象蛋白质交货系统那样的生物医学的应用的大潜力。

简介：我们演示兆兆赫时间域光谱学的应用程序区分guanidine水疗院氯化物的处理导致的叶绿素蛋白质CP43和CP47的符合构造变化，轻照耀并且加热。THz传播光谱学能被用于监视蛋白质变性作用并且以一个可行、有效的方法联系符合构造变化过程，这被显示。

简介：Direct-methodphaseextensionhasbeenappliedtotwo-dimensionalelectrondiffractiondataoftheproteinstreptavidin.Structure-factoramplitudesfromelectrondiffractionwerecombinedwithphasesfromthecorrespondingelectronmicrographs.Maximum-entropydiscriminationandclusteranalysiswereusedtoderiveasolutionfromalargenumberofrandomtrials.Thephaseextensionfrom0.3to0.25nmledtosubstantialimprovementofthereconstructedprojectionimagequality.