简介：Cancerisamajorhealthconcernandleadingburdenoneconomyworldwide.Anincreasingeffortisdevotedtoisolationanddevelopmentofplant-deriveddietarycomponentsaseffectivechemo-preventiveproducts.Phytochemicalcompoundsfromnaturalresourcessuchasfruitsandvegetablesareresponsiblefordecreasingtheriskofcertaincancersamongtheconsumingpopulations.Apigenin,aflavonoidphytochemicalfoundinmanykindsoffruitsandvegetables,hasbeenshowntoexertsignificantbiologicaleffects,suchasanti-oxidant,anti-inflammatoryandmostparticularlyanti-neoplasticproperties.Thisreviewisintendedtosummarizethemostrecentadvancesintheanti-proliferativeandchemo-preventiveeffectsofapigeninindifferentcancermodels.Analysisofthedatafromthestudiedcancermodelshasrevealedthatapigeninexertsitsanti-proliferativeeffectsthroughmultipleandcomplexpathways.Thisguidedustodiscoversomecontroversialresultsabouttheexactroleofcertainmolecularpathwayssuchasautophagyintheanticancereffectsofapigenin.Further,therewerecumulativepositiveevidencessupportingtheinvolvementofcertainpathwayssuchasapoptosis,ROSandDNAdamageandrepair.Apigeninpossessesahighpotentialtobeusedasachemosensitizingagentthroughtheup-regulationofDR5pathway.Accordingtothesepreclinicalfindingswerecommendthatfurtherrobustunbiasedstudiesshouldconsiderthepossibleinteractionsbetweendifferentmolecularpathways.

简介：Thepresentstudywasdesignedtosynthesize2-Cyano-3,12-dioxooleana-1,9(11)-en-28-oate-13β,28-olide(1),alactonederivativeofoleanolicacid(OA)andevaluateitsanti-inflammatoryactivity.Compound1significantlydiminishednitricoxide(NO)productionanddown-regulatedthemRNAexpressionofiNOS,COX-2,IL-6,IL-1β,andTNF-αinlipopolysaccharide(LPS)-stimulatedRAW264.7cells.FurtherinvivostudiesinmurinemodelofLPS-inducedacutelunginjury(ALI)showedthat1possessedmorepotentprotectiveeffectsthanthewell-knownanti-inflammatorydrugdexamethasonebyinhibitingmyeloperoxidase(MPO)activity,reducingtotalcellsandneutrophils,andsuppressinginflammatorycytokinesexpression,andthusamelioratingthehistopathologicalconditionsoftheinjuredlungtissue.Inconclusion,compound1couldbedevelopedasapromisinganti-inflammatoryagentforinterventionofLPS-inducedALI.

简介：AIM:ToevaluatetheeffectofOcimumsanctumleafextractonthedietarysupplementationinthetransgenicDrosophilamodelofParkinson’sdisease.METHOD:TheeffectofOcimumsanctumleafextractwasstudiedonthetransgenicDrosophilamodeloffliesexpressingnormalhumanalphasynuclein(h-αs)intheneurons.O.sanctumextractatfinalconcentrationsof0.0428×10-4,0.87×10-4,and1.85×10-4g·mL-1ofdietwereestablishedandtheflieswereallowedtofeedfor21days.Theclimbingassayandlipidperoxidationweretakenasparametersforthestudy.RESULTS:ThesupplementationofO.sanctumextractshowedadose-dependentsignificantdelayinthelossofclimbingabilityandreductioninoxidativestressinthebrainofPDmodelflies.CONCLUSION:TheresultsofthepresentstudyshowedthattheO.sanctumextractispotentinreducingthePDsymptomsintransgenicDrosophilamodel.

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简介：目的：观察脑维康（NWK）对D-半乳糖致脑老化大鼠模型学习记忆障碍的影响。方法：大鼠背颈部皮下连续注射D-半乳糖生理盐水（80mg/kg）8周建立脑老化大鼠模型,灌胃给予NWK8周。以Morris水迷宫试验检测模型大鼠的空间学习记忆能力,以分光光度法检测乙酰胆碱酯酶（acetylcholinesterase,AChE）、黄嘌呤氧化酶法测定过氧化物歧化酶（superoxidedismutase,SOD）、硫代巴比妥酸法测定丙二醛（maleicdialdehyde,MDA）的变化,以HE染色进行脑组织形态学观察。结果：D半-乳糖致脑老化大鼠在水迷宫中的游泳持续时间、路径长度明显延长。给药8周后,NWK23mg/kg、46mg/kg组大鼠游泳持续时间、路径长度较模型组明显缩短,搜索策略均有改进。与空白组比较,模型组大鼠全脑AChE活力、MDA含量均明显升高,SOD活力明显降低。给药8周后,与模型组大鼠相比,NWK各给药组大鼠全脑AChE活力明显降低,NWK46mg/kg组大鼠全脑MDA含量明显降低,脑组织形态结构有所改善。结论：NWK可改善D-半乳糖致脑老化大鼠模型学习记忆能力。

简介：AnovelPleurotusnebrodensispolysaccharide(PN-S)waspurifiedandcharacterized,anditsimmune-stimulatingactivitywasevaluatedinRAW264.7macrophages.PN-SinducedtheproliferationofRAW264.7cellsinadose-dependentmanner,asdeterminedbytheMTTassay.AfterexposuretoPN-S,thephagocytosisofthemacrophageswassignificantlyimproved,withremarkablechangesinmorphologybeingobserved.FlowcytometricanalysisdemonstratedthatPN-SpromotedRAW264.7cellstoprogressthroughSandG2/Mphases.PN-Streatmentenhancedtheproductionsofinterleukin-6(IL-6),nitricoxide(NO),interferongamma(INF-γ),andtumornecrosisfactor-α(TNF-α)inthemacrophages,withup-regulationofmRNAexpressionsofinterleukin-6(IL-6),induciblenitricoxidesynthase(iNOS),interferongamma(INF-γ)andtumornecrosisfactor-α(TNF-α)beingobservedinadose-dependentmanner,asmeasuredbyqRT-PCR.Inconclusion,theseresultssuggestthatthepurifiedPN-Scanimproveimmunitybyactivatingmacrophages.

简介：枸杞乙醇提取物100mg／kg、500mg·d×50d对D-半乳糖所致衰老小鼠学习记忆能力的下降有明显提高作用，并可减少心、肝、脑组织脂褐质浓度，提高RBCSOD活力，表明枸杞改善记忆的作用与其促进体内自由基的清除有关。

简介：小鼠scD-半乳糖40mg/kg·d×40d或在臭氧(0.53±0.12mg/m~3)环境中动式染毒8h/d×15d造成亚急性衰老模型。与此同时ig水解珍珠粉溶液50、100或200mg/kg·d。结果表明,与D-半乳糖组相比,水解珍珠粉100、200mg/kg可显著提高全血GSH-Px的活性,200mg/kg可显著降低血清MDA的含量,100、200mg/kg可以显著降低心脏脂褐素的含量。3个剂量均可显著降低脑脂褐素的含量。与臭氧组相比,珍珠粉3个剂量组均可显著提高全血GSH-Px的活性,100、200mg/kg可显著降低血清MDA的含量,100mg/kg组能显著降低肝脂褐素含量,心脑脂褐素含量的变化不显著。