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简介：TheaimofthisstudyistoexplorethegenomicmolecularorganizationandgenogroupofhumannorovirusfrominfectedinfantsinGuangzhouofChina.PrimersweredesignedaccordingtothegenomicsequenceofnorovirusintheGenBank,andthenorovirusgenomewasamplifiedbyRT-PCR.ThePCR-productswereclonedintoTvectorandsequenced,andthegenomicnucleotidesequenceswereanalyzedwiththeprogramsCLUSTALW/X,DNASTARandRAT(RecombinationAnalysisTool).TheNVgz01straingenomeis7558bpinlengthandencodesthreeopenreadingframes(GenBankaccessionNo.isDQ369797).ThegenomicsequencesofNVgz01werecomparedwiththoseofnomvirusinGenBank,whichrevealedthatthehomologywithgenogroupⅡrangesbetween76%-90%,andgenogroupⅠbe-tween43%-44%.TheORF1regionshared94%and88%identitywithMc37andFarmingtonstrains,respectively;thecapsidregion(ORF2)shared65%and94%identitywithMc37andFarmingtonstrains,respectively.Phylogenetictreeswerereconstructedbytheneighbor-joiningmethod.ComparativecompletesequenceanalysisoftheNVgz01withreportedhumannorovimsgenomicsequencesrevealedthatthisisolatebelongstogenogmupⅡ.TheORF1andORF2regionsshareddifferentidentitywithMc37andFarmingtonstrains,suggestingNVgz01couldbearecombinantvires.

简介：ToanalyzethegenomicmolecularstructureandgenotypeofhumanastrovirusisolatedfrominfantinGuangzhouofChina,theprimersweredesignedbasedonthegenomicsequenceofastrovirusfromtheGenBankandthetargetsequencewereamplifiedbyRT-PCR.ThenthePCR-productswereclonedtoTvectorandsequenced.ThegenomicnucleotidesequenceswereanalyzedbytheprogramsCLUSTALWandDNASTAR.ItwasfoundthatthefullgenomiclengthofHASTVgz01strainwas6721bpandtheORFswere6558bp.The5'and3'UTRwere82and81nucleotides.Thegenomeincluded3openreadingframes(ORFs):ORF1a,ORF1bandORF2.The5'-terminalORF1astartedatnucleotide83andextendedtonucleotide2845.ORFlb(nt2785tont4332)overlapedORFlaby61nucleotides.The3'-terminalORF2beganatnucleotide4325andterminatedatnucleotide6640.ORF2had2316nucleotides.ComparedwithotherastrovirussequencesinGenBank,thehomologyoftheaminoacidsequenceofORF2ofHASTVgz01strainwiththatofserotype4was93%.Homologywithotherserotypesrangedfrom61%to70%.ThecompletenucleotidesequenceofastrovirusHASTVgz01strainisolatedfromGuangzhouinChinawas6721bpinlength,GenBankaccessionNO.DQ344027.ComparingtheORF2ofastrovirusHASTVgz01withtheknownsequencesoftypes1-8thehighesthomologywasserotype4(93%).ComparativesequenceanalysisoftheHASTVgz01ORF2withthereportedhumanastrovirussequencesrevealedthattheisolatedastrovirusbelongstogenotype(serotype)4.

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简介：【摘要】目的 分析肥东县梅毒的流行病学特征，为制定梅毒防控措施提供重要的科学依据。 方法 流行病学“三间”分析的研究方法。 结果  2015-2020年肥东县共报告3239例梅毒，梅毒发病率总体呈上升趋势，六年发病率升幅为99.86%，六年各年度间报告发病率差异有统计学意义（χ2=213.904，P0.001）；六年报告数居前六位的职业依次为农民、家务及待业、商业服务、离退人员、工人、其他，六种职业共报告病例3125例，占梅毒报告总数的96.48%；六年的地区分布中，居前十位的依次为店埠镇、撮镇镇、梁园镇、白龙镇、八斗镇、古城镇、石塘镇、长临河镇、牌坊乡、众兴乡，前十位总数为2531例，占六年发病总数的78.14%。 结论 2015-2020年肥东县梅毒发病率上升明显，升幅高达99.86%，说明我县梅毒疫情形势在较长时间内依然十分严峻，必需继续加大加强我县梅毒的防治工作，尤其要加强对高危人群的健康教育重点干预工作，即加强对梅毒性病防治知识的宣传，从而有效遏制梅毒的高流行状态。