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简介：Objective:ToanalyzemoleculartrendsoftheHIVepidemicinShenzhen.Methods:SerumcollectedfromShenzhenAIDSpatientsbetween1992-1999wasanalyzedusingmoleculartechniques.DNAfragmentsoftheHIV-1EnvgenewereamplifiedbynestedPCRfromunculturedperipheralbloodmononuclearcells(PBMCs)fromtheseserumsamples.TheC2-C3regionoftheEnvgenewassequencedandanalyzed.Specifichigh-riskbehaviorswerealsoanalyzed.Results:WefoundthatthetransmissionofHIVinthecitywasmainlythroughsexualbehaviors(46.0%).TherewerefourHIV-1subtypes:B′,B,CandEwith6.31%,7.95%,3.09%and8.92%genedivergenceinsideeachsubtypeinShenzhen.Theseresultssuggestedthatepidemictimeswere6,8,3and9respectively.ThemainepidemicsubtypeswereEandBstrains.AIDSpatient''''''''santigenicvariationwasslightlyhigherthanthatofHIVinfectedindividuals.Conclusion:SurveillancedatareflecttrendsandtheepidemictimeofHIV,whichwillbeusefulforpolicymakerstoformulateeffectivestrategiesofHIV/AIDSpreventionandcontrolinShenzhen.

简介：Objective:TomakeasummaryofthetrendsofHIV/AIDSepidemicandrouteoftransmissioninShenzhenfrom1992to2000toprovidescientificevidenceforthepolicy-makertoformulatestrategiesofHIV/AIDSpreventionandcontrolinShenzhen.Method:ToanalyzeHIV/AIDSsurveillancedatainShenzhenfrom1992to2000bysoftwareEpi-Info.Results:OnehundredandtwoHIVpositivecasesincluding18AIDScases(threecasesdeceased)wereidentifiedinShenzhenfrom1992to2000.Amongthe102cases,thoseinfectedviasexwere46.0%,intravenousdruguse42.9%.Blooddonation7.8%,bloodtransfusionrecipient3.9%,andindeterminate2%.Conclusion:InShenzhen,HIV/AIDSinfectionisspreadingandincreasingrapidly.EffectivepreventionmeasuresmustbetakentoreducetheHIVprevalenceassoonaspossible.

简介：Objective:Toclone,sequenceandexpresstheprimateβ-chemokineRANTESgenes,hRANTESfromH.sapiensandmRANTESfromM.Mulatta,inordertoexplorethepossibilityofAIDSgenetherapy.Methods:hRANTESandmRANTESwereamplifiedbyreversetranscription-polymerasechainreaction(RT-PCR)fromRNAsextractedfromphytoagglutinin(PHA)-activatedperipheralbloodlymphocytes,hRANTESwascloned,sequencedandexpressedinvitro,andmRANTESwasdirectlysequencedforhomologycomparison.Results:Anexpected276bpfragmentwasobtainedinbothamplifications,andsequencedatademonstratedarelativelyhighhomologyamongdifferentcopiesofhRANTES(97%),andhRANTESwasupto95.6%homologoustomRANTES.WhencomparedwithRANTESfromothermammals,hRANTESgaverisetoahomologyrangingfrom77%to86%.TheclonedhRANTESwasexpressedinvitroandapositivesignalofRANTESwasdetectedbydotblotting.Conclusion:Thefull-lengthofhRANTESsequencewassubmittedtoGenBankandhadbeenreleased.OurmRANTESsequenceisfirstreportedandnotyetappearedinGenBank.ThesuccessfulcloningandexpressionofhRANTESwillprovideabasisforAIDSgenetherapyinthefuture.

简介：Epidemicsofsexuallytransmitteddiseaseshavebecomeworldwideseriousproblems.TheInstituteofDermatologyandVeneologyofWenshanAutonomousPrefectureinYunnanisacenterofpolitical,economical,culturalandsocialactivitiesintheprefecture.ItisalsothelocationoftheCommitteeofWenshananditsgovernment.WenshandataindicatesthatSTDshavebeenspreadingfromtownsofthisprefecturetothecountryside.

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简介：Objectives:TounderstandtrendsandepidemiologicalfeaturesofsyphilisovertherecentyearsinChinaandprovideascientificbasisfordevelopingpreventionstrategies.Methods:From1985to2000,syphiliscase-reportingdatacollectedfromallprovinces,autonomousregionsandmunicipalitieswereanalyzedbyapplyingepidemiologicalmethods.Results:(1)Epidemictrends:syphilisincidencehassteadilyriseninChinafrom1985to2000,especiallyafter1993,whenitassumedanexponentialgrowthpattern.80,406casesofsyphiliswerereportedinthecountryin1999,whichwasalmost40timesthenumberreportedin1993.Duringtheperiodof1993-1999,theannualaveragegrowthofthesyphilisincidenceratewas83.55%.Perhapsduetoarecentnationallawenforeementcampaign,thenumberofreportedsyphiliscasesdroppedslightlyin2000.(2)Geographicaldistribution:Syphilisspreadfromcoastal,""open""cities(especiallysomecitiesinFujianprovince)toinlandurbanareas,thentoruralareas.RegionswithahighincidencerateofsyphilisinChinaweretheMinjiang,Yangtze,andZhujiangRiverDeltas,BeijingandTianjinmunicipalities,andNortheastChina.Therewasasignificantdifferenceofsyphilisincidenceratesandgrowthratesbetweentheseareas.Aseriousepidemicoccurredinsomeareas,withanincidenceratereachingover200casesoutof100,000.(3)Populationdistribution:theratioofmaleandfemalecasesgraduallychangedfrom1.57:1in1993to1.02:1in2000.Theratewasthehighestinthe20-29agegroupandthelowestin10-14agegroup.Agreatdifferenceexistedinprevalencebetweendifferentpopulationgroupsanddifferentareas,andsomeareaswithseriousepidemicshadhighprevalenceratesevenamoungthegeneralpopulation.(4)Clinicalstageofsyphilis:Primaryandsecondarysyphiliscaseshavebeendominatingwithapercentageof90%ofallsyphiliscases.Primarysyphiliswasthemostcommoninmalesandsecondarysyphilisinfemales.Secondaryandlatentsyphilisc

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简介：ChinesepopulationsinfectedwithHIV-1.Methods:GenomeDNAfromperipheralbloodmononuclearcells(PBMCs)of78HIV-1infectorswasamplifiedbypolymerasechainreaction(PCR).CCR5,CCR2bandSDF1genefragmentswereobtainedfromrestrictivefragmentlengthpolymorphism(RFLP)and/orCCR△32,CCR5m303,CCR2b-64IandSDF1-3'Aallelicgenes'mutationalfrequenciesweresequenceddirectlyfromPCRproducts.Results:NoneofCCR5△32,CCR5m303genemutationwerefoundin78subjectswithHIV-1infection.TheallelicgenemutationfrequenciesofCCR2b-64IandSDF1-3'Acorrespondingto14.9-34.0%and17.6-38.2%of95%CI,were22.79%and26.92%respectively.TheircolonydistributionconformedtotheHardy-Weinbergequilibrium.Conclusion:TheHIV-1infectionsfoundatpresentareallsusceptiblepopulationofCCR5△32andCCR5m303.ThepolymorphismandfrequenciesofCCR5△32,CCR5m303,CCR2b-64IandSDF1-3'AallelesfromChineseHIV-1infectedpopulationweredisclosedinthisstudyforthefirsttime,whichisofsignificanceforstudyingthegeneticresistancetosusceptibilitytoHIV-1infectionaswellasAIDSdiseaseprogression.

简介：Objective:TostudythecellularimmunityfunctionofpatientswithearlysyphilisandtheeffectsonimmunemodifiersEsberitoxNorIFN.Methods:T-lymphocytesubpopulationsoftheperipheralbloodin44patientswithsyphilisand40healthycontrolswereexaminedbyflowcytometry.Results:ThenumberofCD4+cellsandtheCD4+/CD8+ratioinpatientswithsyphiliswerefoundtobesignificantlylowerthanthoseinthecontrol(P<0.01),whilethenumberofCD8+cellswashigherthanthatinthecontrol(P<0.01).TheCD4+/CD8+ratiointhosewithactivediseasewaslowerthanthatinthosewhohadbeencured(P<0.05).TheCD4+countandtheCD4+/CD8+ratiointhosetreatedwithantibioticsalone(PenicillinGorCephalosporins)werelowerthanthosetreatedwithbothantibioticsandimmunomodulators(P<0.05).Conclusions:Cellularimmunityinthepatientswithearlysyphiliswasprominentlysuppressed,andtreatmentwithimmunomodulatorsmaybehelpfulfortherecoveryofcellularimmunityofthesepatients.

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简介：Objective:Toinvestigatetheprevalenceofmycoplasmainfectionsandthesensitivitytoantibioticsamongpatientswithnongonococcalurethritisorcervicitis(NGU)inChongqing.Methods:387NGUcaseswithmycoplasma-positiveresultsuponculturewereanalysedretrospectively.RESULTS:Themajorityofpatientswithmycoplasmainfectionswereinthe20-40yearoldagegroup.Nosignificantdifferencewasfoundbetweenmalesandfemales.UreaplasmaurealyticumisthemainpathogenoftheseNGUcasesandnoclearrelationshipbetweenitsconcentrationandpathogenicabilitywasnoted.Drugsensitivitywastestedagainstnineantibiotics;thesensitivityratestojosamycin,minocyclineanddoxycyclinewere94.06%,88.89%and86.82%respectively,whiletheresistanceratestolincomycin,ofloxacin,azithromycinandroxthromycinwere74.94%,42.12%,41.60%and40.31%inturn.Conclusions:Josamycin,minocyclineanddoxycyclinecouldbeusedasthefirstchoicetotreatNGUwithmycoplasmainfectionsinChongqing.ItisimportanttoselectantibioticsforNGUtreatmentwithmycoplasmainfectionsbasedontheresultsofdrugsensitivitytests.

简介：Objective-TocomparetheconsistencyoftheresultsfromdetectingHIV-1antibodyinthepairedurineandserumspecimensfromdrugusersbyELISA.Methods:Thepairedurineandserumspecimensfrom273drugusersdetainedatadetoxificationunitwerecollected,andtheHIV-1antibodiesinthespecimensofthemwerescreenedbyurineandserumELISAkits,respectively.Results:Of273serumspecimens,94onesshowedpositivereactionandamong94counterparturinespecimens,93onesalsoappearedpositivereaction.Takingtheresultstogether,theconsistentrateofHIV-1antibodyscreenedbyurineandserumELISAkitswas99.6%.Conclusion:TheurineELISAkit,whichscreenedHIV-1antibodyofurineshowingalmostthesameresultstestedbyserumELISAkit,isreliable.ItisproposedthaturineELISAbeintroducedinmanyfields.

简介：Objective:TounderstandthechangeofTh1/Th2andTc1/Tc2balanceinpatientswithsecondaryearlysyphilis.Methods:PeripheralbloodThandTccellsubpopulationswereexaminedbythree-colorflowcytometryin30patientswithsecondaryearlysyphilisand20healthysubjects.Results:PercentagesofCD3^+,CD4^+TcellsandtheratioofCD4^+/CD8^+andNKcellsinsyphiliticpatientsweresignificantlylowerthaninthecontrolgroup(P<0.001),butnostatisticaldifferencewasfoundinpercentagesofCD8^+andCD19^+cellsbetweenthetwogroups(P>0.05).ThepercentageofTh1cellsandratiosofThl/Th2andTcl/Tc2insyphiliticpatientswereclearlydecreasedascomparedtothoseincontrois(P<0.001-0.01),whereaspercentagesofTc0andTc2cellsinsyphiliticswashigherthanthoseincontrols(P<0.001).Conclusion:Therecouldbeadisturbanceofcellularimmunityandtherelativelyintactfunctionofhumoralimmunityinsecondarysyphilis.ItispostulatedthatthepolarizationtowarddevelopmentofTh2andTc2cellsandtheirassociatedcytokinesmightbeinvolvedintheincompleteclearanceoftreponemesandthedevelopmentofchronicinfection.