简介:针对手动挡车辆在运行过程中无挡位传感器的情况下挡位信号无法识别获取的问题,通过对多次整车转鼓试验进行分析,发现读取CAN总线里面的速比信息,然后基于直方图计算各速比范围信号出现的频率,并以此确定挡位数目和速比的大小范围,再利用Parzen窗函数的方法获取各挡位的实际速比,可以实现对挡位信号精准的识别。试验结果表明,采用Parzen窗的挡位识别方法能够识别出挡位信号。对比Parzen窗获取的挡位识别信息与挡位信号传感器直接获取的挡位信息,发现两者的相似度很高,这也验证了该方法的可行性和准确性。该方法提供了整车运行过程中一种挡位识别的新方式。
简介:新的钻井工艺或钻井液体系的使用在保证钻井施工顺利进行的同时,也给录井岩屑的岩性识别带来了极大的挑战,其中以膏盐岩地层尤为突出。以塔里木油田大北X井为例,提出了主成分分析(PCA)与径向基函数(RBF)神经网络相结合的膏盐岩地层岩性识别方法,利用主成分分析法去除指标变量间的相关性,将原始指标变量重新线性组合为4项综合变量作为RBF神经网络的输入向量;最后建立适于识别膏盐岩地层岩性的RBF神经网络模型。实际识别结果表明,该PCA-RBF神经网络模型对于膏盐岩地层岩性的识别具有较高的准确性,完全可以满足实际应用的要求,具有进一步推广的价值。
简介:目的:分析物种间miRNA序列的共同点和差异点,为后续miRNA研究奠定基础。方法:从miRBase数据库下载8种模式生物,即智人、小鼠、大鼠、果蝇、线虫、拟南芥、水稻、玉米的全部miRNA,通过生物信息学相关软件及方法对其进行分析。结果:各物种成熟miRNA序列长度均约为22nt,植物miRNA长度分布范围较动物更集中;而pre-miRNA则相反,植物pre-miRNA的长度变异远大于动物;各物种miRNA序列第一个碱基倾向于U,而其他位点的碱基在不同物种间变异较大;miRNA的保守性有一定的范围,不存在在所有物种中均保守的miRNA。结论:找到了miRNA间的一些共同点及差异点,可为后续miRNA鉴定注释提供借鉴。
简介:BackgroundAlthoughmiRNAshavebeenshowntoassociatewithavarietyofdiseases,whethermiRNAsinmonocyteassociateheartfailure(HF)hasbeennotwellstudied.MethodsEightpatientswithischemicHF(IHF),8patientswithnon-ischemicHF(NIHF)and8healthyvolunteerswererecruited.Clinicalcharacteristicsofallparticipantswerecollected.PeripheralbloodsamplesweredrawnforanalysisofmiRNAexpressioninmonocytes.ResultsAllparticipantsweremaleandtheparticipantsinIHFgroupwereolderandhadhigherpercentageofsmokeranddiabetesmellitusthanintheothertwogroups(P<0.05).SerumlevelsofcreatinineandNT-proBNPweresignificantlyhigherinIHFpatientscomparedtotheothertwogroups(P<0.05).MoreparticipantsinIHFgroupweretreatedwithACEI/ARB,beta-blockerandstatins.ParticipantswithNYHAgradeIIIaccountedfor62.5%inIHFgroup,whileparticipantswithNYHAgradeIVaccountedfor87.5%inNIHFgroup.Thelevelsof11miRNAsinmonocytesweresignificantlyhigherintheIHFgroup,andthelevelsof7miRNAsweresignificantlyincreasedintheNIHFgroup.OtherdifferencesinmiRNAslevelsbetweenIHFandNIHFgroupswerealsoobserved.ConclusionourpresentstudyrevealedthattherearesubstantialdifferencesinmiR-NAsbetweenHFpatientsandhealthyvolunteer.
简介:BackgroundStudieshaveshownthatmiRNA-155playedanimportantroleintheprocessofdevelopmentofhypertension.However,thereisnodateaboutmiRNA-155andbloodpressuremonitoringparameters.Therefore,weexaminedwhetherinhypertensivepatientstheexpressionlevelofplasmamiRNA-155relatedto24-hambulatorybloodpressuremonitoring(ABPM)parameters.MethodsAcohortofadultpatientsscheduledtoreceivephysicalexamination,officeandambulatorybloodpressuremonitoring.Quantitativereversetranscriptasepolymerasechainreaction(qRT-PCR)wasusedtoevaluatetheexpressionofselectedmiRNA-155.ThemiRNA-155expressionlevelcorrelationbetweenbloodpressureparameterswasassessedusingtheSpearmancorrelationcoefficient.ResultsFiftyfouressentialhypertensionpatients(25men;meanage,53.28±9.52years)andthirtyhealthyvolunteers(15men;meanage,53.03±5.87years)wereincluded.WeobservedhigherexpressionlevelofmiRNA-155(32.31±2.85vs27.21±1.59,P<0.001)inhypertensivepatientscomparedtohealthycontrolindividuals.MiRNA-155expressionlevelshowedsignificantpositivecorrelationwith24hDaytimeSBP(r=0.681,P<0.001),24hDaytimeDBP(r=0.473,P<0.001),24hDaytimePP(r=0.565,P<0.001)anddipping(r=0.257,P=0.018),respectively.ConclusionsOurstudyshowedthatmiRNA-155expressionlevelwasassociatedpositivelywithdaytimebloodpressuremonitoringparameters,aswellasbloodpressurevariability,indicatingapossibleimplicationofmiRNA-155inthepathogenesisofhypertension.