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42 个结果
  • 简介:Viaseveraltechniquestomodifysensitivematrixforinhabitingtheleakageoffluorescentindicator,anewstablesensingmaterialformonitoringphosphateshasbeenpreparedandappliedtothemeasurementofphosphatesinartificialseawater.ItisbasedonthereactionofPO3-4withAl(Ⅲ)-Morinthatleadstothefluorescencequenchingofthecompositematrix.AtpHvalue4.0andthesalinityvalue25.0,theresponsetimeofoutputsignalhavingreachedthesteadystateislessthan300seconds.ItscalibrationgraphisgainedintherangeofH2PO-4massconcentrationfrom1.50to7.00μg/mLwiththelimitofdetection(3σ/K)0.02μg/mL.Whenitwasusedtomeasurephosphatesinartificialseawater,therecoveryrangedfrom96.78%to102.09%.Beingstoredunderthepropercondition,themembranesmaintainsensitivefor90-120days,andareabletobeusedfor50-80timeswithindicatorsupplement.

  • 标签: 磷酸盐 荧光材料 荧光淬灭 铝-桑色素 人造海水
  • 简介:Theobjectiveofthestudywastoelucidateopticalcharacteristicsofthechromophorestructuresoffluorescentproteins.RamanspectraofcommonlyusedGFP-likefluorescentproteins(FPs)withdiverseemissionwavelengths(green,yellow,cyanandred),includingtheenhancedhomogenousFPsEGFP,EYFP,andECFP(fromjellyfish)aswellasmNeptune(fromseaanemone)weremeasured.High-qualityRamanspectrawereobtainedandmanymarkerbandsforthechromophoreoftheFPswereidentifiedviaassignmentofRamanspectrabands.WereportthepresenceofapositivelinearcorrelationbetweentheRamanbandshiftofCs=C6andtheexcitationenergyofFPs,demonstratedbyplottingabsorptionmaxima(cm-1)againstthepositionoftheRamanbandCs=C6inEGFP,ECFP,EYFP,theanionicchromophoreandtheneutralchromophore.ThisstudyrevealednewRamanfeaturesinthechromophoresoftheobservedFPs,andmaycontributetoadeeperunderstandingoftheopticalpropertiesofFPs.

  • 标签: RAMAN SPECTRA FLUORESCENT protein CHROMOPHORE
  • 简介:在这个工作,新奇bisbenzimidazolylpyridine-functionalized荧光灯环氧基树脂树脂为organophosphate杀虫剂被综合察觉。环氧基树脂树脂被Fourier变换红外线的光谱学(英尺红外)描绘,质子原子磁性的回声光谱学(1HNMR),胶化浸透层析(GPC),微分扫描热量测定(DSC)和荧光光谱学。在与Eu(III)离子装载以后,环氧基树脂树脂显示出强壮的荧光排出物。当暴露了在答案跟踪diethylchlorophosphate的数量时,荧光排放被观察即刻地被熄灭。熄灭常数K为在617 熄灭的sv;nm决心是0.377 ;× ;103 L/mol。这敏感熄灭排放的功能和聚合支持的容易的处理自然使树脂能是organophosphates的察觉的一个有希望的chemosensor候选人。

  • 标签: 有机磷酸酯 环氧树脂 农药检测 荧光光谱 傅立叶变换红外光谱 质子核磁共振波谱
  • 简介:Afeasiblemethodofcombiningtheconceptoffluorescencehalf-lifeandthepowerdependentphoto-bleachingrateforcharacterizingthepracticalphotostabilityoffluorescentproteins(FPs)wasintroduced.Furthermore,byusingafluorescentphotostabilitystandard,arelativecomparisonofthephotostabiltyofFPsfromdifferentresearchgroupswasproposed,whichwouldbeofgreatbenefitfordevelopingnovelFPswithoptimizedemissionwavelength,betterbrightness,andimprovedphotostability.Weusedrho-damineBasanexampletoverifythismethodandevaluatethepracticalphotostabilityofafar-redFP,mKate-S158C.Experimentalresultsindicatedgoodpotentialofthismethodforfurtherstudy.

  • 标签: 荧光 蛋白质 耐光性 稳定性
  • 简介:Anegativeunipolarpulsedvoltageisappliedtostudyinternalelectricalparametersofthexenonfilleddielectricbarrierdischarge(DBD)sources.TheVUVradiationemittedfromthesesourcesischaracterizedbymeansofthephotoluminescenceintensityoftheredphosphorpellet.TheredphosphorconvertstheVUVradiationintovisibleradiationandtheemissionspectraincludeapeakat619.56nm.Theemissioncharacteristicsoftheredphosphorareanalyzedintermsofthepressure-distance(pd),risetimeandfrequencyofthepulsedvoltagewaveform.Theemissionintensitymeasuredatdifferentoperationalconditionsconfirmsthattheformationanddecayofthexenonexcimer,Xe2*,increasewiththeincreaseinreducedelectricfield,E/N.AfterexceedingcertainlimitsofE/N,theintensityofXe2*decreasesrapidly.

  • 标签: 真空紫外辐射 荧光灯 红色荧光粉 表征 介质阻挡放电 脉冲电压
  • 简介:Parameterquantificationprincipleofthree-dimensional(3-D)fluorescentspectrumanditsrealizationtechniquearestudied.Thepotablefiberopticfluorescentmeasurerformineraloilisgiven.Threeparametersoftheexcitinglightwavelength.theeavelengthandtheintensityofthefluorescentlightexcitedbythemeasuredoilareexpressedwith3-Dcoordinatesandareprocessedsoastobesampledagainandtobecurvefitted.

  • 标签: 荧光光谱 指纹图形 检测系统 矿物油
  • 简介:Primordialgermcells(PGCs),asprecursorsofmammaliangermlineage,havebeengainingmoreattentionasanewresourceofpluripotentstemcells,whichbringagreatpossibilitytostudydevelopmentaleventsofgermcellinvitroandatanimallevel.EG4cellsderivedfrom10.5dayspostcoitum(dpc)PGCsof129/svJstrainmousewereestablishedandmaintainedinanundifferentiatedstate.WithanattempttostudythedifferentiationcapabilityofEG4cellswithareporterprotein:greenfluorescenceprotein,andthepossibleapplicationofEG4cellsintheresearchofgermcelldevelopment,wehavegeneratedseveralEG4-GFPcelllinesexpressingenhancedgreenfluorescenceprotein(EGFP)andstillmaintainingtypicalcharacteristicsofpluripotentstemcells.Then,thedifferentiationofEG4-GFPcellsinvitroaswellastheirdevelopmentalfateinchimericembryoswhichwereproducedbyaggregatingEG4-GFPcellsto8-cellstageembryoswerestudied.TheresultsshowedthatEG4cellscarryinggreenfluorescencehaveapotentialuseintheresearchofgermcelldevelopmentandotherrelatedstudies.

  • 标签: 胚胎生殖细胞 嵌合体 发育结局 基因转染 EGFP 绿色荧光蛋白
  • 简介:Wereportonanoveldibenzothiophene-basedtwo-photonfluorescentprobeforselectivenuclearbioimaging,whichcontainsbilaterallysymmetricalpyridineringsconnectedbyacentralconjugated-bridgedibenzothiophene.Thisprobepossessesalargetwo-photonabsorptioncross-sectionof471GM,yieldsa25-foldenhancementofthefluorescencetitration,andastrongerphotostabilityfornucleilabelingthanexistingprobes.Thereal-timeobservationperiodisaminimumof1800sunderafemtosecondlaserexcitation,whichissignificantlylongerthanthatof40,6-diamidino-2-phenylindole.Theaboveresultsconfirmthatthisnovelmoleculeisasuitabletwo-photonfluorescentprobeforapplicationtonuclearbioimagingincells.

  • 标签: 双光子吸收截面 荧光探针 生物成像 应用 细胞 二苯并噻吩
  • 简介:AgreatamountoffoodbornepathogenswereGram-positive(G?)bacteria,athreattopublichealth.Inthisstudy,consideringthebindingabilityofnisintowardsG-bacteriaandthestablefluorescentabilityofEGFPprotein,afluorescentnisin–EGFPproteinprobewasconstructedbyageneengineeringmethod.NisinandEGFPwereusedasthereceptorandfluorophore,respectively,todetectG?bacteria.ThenisinandegfpgenewereamplifiedseparatelyaccordingtothesequencepublishedinGenBankusinguniqueprimers.ThetwogeneswereclonedintoapET-28b(?)vectorresultinginapET-28b(?)–nisin–egfpvector.ThevectorwastransferredintoEscherichiacoli(E.coli)BL21(DE3)forexpression.Theexpressedproteinwasextracted,purifiedbyaNi–NTAcolumn,andthentestedbytheSDS-PAGEmethodtoconfirmitsmolecularweight.Listeriamonocytogenes(L.monocytogenes),Staphylococcusaureus(S.aureus),andMicrococcusluteus(M.luteus)wereusedastherepresentationsofG?bacteria.E.coliO157,representingthegram-negative(G-)bacteria,wasusedasanegativecontrol.Thebindingspecificityoftherecombinantproteinwasperformedontwotypesofbacteriaandthendetectedthroughfluorescentmicroscopy.Theresultsindicatedthatthenisin–EGFPprobecoulddetectG?bacteriaat108CFU/mL.

  • 标签: Nisin–EGFP GRAM-POSITIVE bacteria Fluorescent DETECTION PROBE
  • 简介:Genomicresearchhasmadealargenumberofsequencesofnovelgenesorexpressedsequencetagsavailable.Toinvestigatefunctionsofthesegenes,asystemforconditionalcontrolofgeneexpressionwouldbeausefultool.Inducibletransgeneexpressionthatusesgreenfluorescentproteingene(gfp)asareportergenehasbeeninvestigatedintransgeniccelllinesofcotton(COT;GossypiumhirsutumL.),Fraserfir[FRA;Abiesfraseri(Pursh)Poir],Nordmannfir(NOR;AbiesnordmannianaLk.),andrice(RIC;OryzasativaL.Cv.Radon).Transgeniccelllineswereusedtotestthefunctionofthechemicalinducerdexamethasone.Inducibletransgeneexpressionwasobservedwithfluorescenceandconfocalmicroscopy,andwasconfirmedbynorthernblotanalyses.Dexamethasoneat5mg/Linducedgfpexpressiontothenearlyhighestlevel48haftertreatmentinCOT,FRA,NOR,andRIC.Dexamethasoneat10mg/LinhibitedthegrowthoftransgeniccellsinFRAandNOR,butnotCOTandRIC.Theseresultsdemonstratedthatconcentrationsofinducerforoptimuminduciblegeneexpressionsystemvariedamongtransgeniccelllines.Theinduciblegeneexpressionsystemdescribedherewasveryeffectiveandcouldbevaluableinevaluatingthefunctionofnovelgene.

  • 标签: 地塞米松 绿色荧光蛋白 基因 表达 转基因植物 细胞
  • 简介:ZnOnanotubeswerehydrothermallysynthesizedandtheZNTs/CeO2fluorescentcompositewerepreparedbyintroducingnanoCeO2particlesintoZnOnano-tubesviaachemicalsolutionadsorptionandannealingprocess.ThesampleswerecharacterizedbyX-raydiffraction,scanningelectronmicroscopy,Fouriertransformationinfraredspectroscopyandroomtemperaturephotoluminescencemeasurement.DuetotheinteractionbetweenCe4+andthesurfaceatomsofZnO2nano-tubes,aphotoluminescenceenhancementwasobser...

  • 标签:
  • 简介:Novelmolecularmaterial,1-benzothiazoly-3-pheny1-pyrazoline(BTPP)wasfoundtofunctionasbrightbluelightemittingdyeinorganicelectroluminescentdevice,anditsopticalandelectriccharacteristicswereinvestigated.Thisheterovycliccompoundexhibitedgoodcharacteristicsofbluephotoluminescenceandelectroluminescence,whichhadtheemissionpeakat450nm.Thesinglelayerlight-emittingdevicesusingBTPPaslight-emittingmaterialdispersedinpoly(N-vinylcarbazole)(PVK)anddoublelayeronesusingPBDasholeblocklayerabovethelight-emittinglayerwerefabricatedusingconventionalspin-castingandvaccumvapourdepositionmethods.TheintroductionofPBDhasenhancedelectroninjectionandluminanceefficiency,comparedwiththesinglelayerLEDs.

  • 标签: 杂环化合物 蓝光二极管 荧光染料
  • 简介:扩大来源的阶段信息与一个环形的二地区的阶段唯一的过滤器被重建在一荧光灯的共焦的扫描为分辨率的光显微镜改进。它来源尺寸上并且在过滤器的外部环形的地区的阶段传播上的决定的依赖被它的三维的光转移功能(3DOTF)的使用理论上调查。增加的来源尺寸和为光系统的最佳的3DOTF的阶段唯一的过滤器的外部环形的地区的要求的阶段价值被介绍。CLC数字TH742

  • 标签: 成像特性 荧光共焦显微镜 滤光器 相位信息