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简介：Object:ToidentifytranscriptvariantsandexpressionpatternsofporcineMitf.Materialsandmethods:ApairwiseBLASTsearchatNCBIdatabasewasperformedtodeducethestructureofporcineMitfgene.Subsequently,50RACEandfluorescentquantitativeRT-PCRwereusedtoanalyzetheexpressionpatternofporcineMitfindifferenttissues.Results:FourtranscriptvariantsofporcineMitf,MITF-A,MITF-H,MITF-MandMITF-SUSwereidentified,allsharinghighhomologywiththoseinhumans,exceptMitf-SUS.Conclusion:ThesequenceofporcineMitfappearhighlyhomologoustohumanMITF.However,only4transcriptvariantsofporcineMitfwereidentifiedintheseminipigs,lessthanthe9transcriptvariantsinhumanMITF.

简介：Inthispaper,weprovethatforholonomicnonconservativedynamicalsystemthePoincareandPoincaré-Cartanintegralinvariantsdonotexist.Insteadofthem,weintroducetheintegralvariantsofPoincaréCartan’stypeandofPoincaré’stypeforholonomienoneonservativedynamicalsystems,andusethesevariantstosolvetheproblemofnonlinearvibration.Wealsoprovethattheintegralinvariantsintro-ducedinreferences[1]and[2]aremerelythebasicintegralvariantsgivenbythispaper.

简介：Magnificent&GorgeousTheShanghaiCommunication&FinanceMansiondesignedbyWangShiwei,GeneralDesigner,wontheSpecialAwardinthe“2004DongpengInteriorDesigningCompetition”organizedbytheChinaConstruction&DecorationAssociation.

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简介：AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, generating new variants that pose a threat to global health; therefore, it is imperative to obtain safe and broad-spectrum antivirals against SARS-CoV-2 and its variants. To this end, we screened compounds for their ability to inhibit viral entry, which is a critical step in virus infection. Twenty compounds that have been previously reported to inhibit SARS-CoV-2 replication were tested by using pseudoviruses containing the spike protein from the original strain (SARS-CoV-2-WH01). The cytotoxicity of these compounds was determined. Furthermore, we identified six compounds with strong antagonistic activity against the WH01 pseudovirus, and low cytotoxicity was identified. These compounds were then evaluated for their efficacy against pseudoviruses expressing the spike protein from B.1.617.2 (Delta) and B.1.1.529 (Omicron), the two most prevalent circulating strains. These assays demonstrated that two phenothiazine compounds, trifluoperazine 2HCl and thioridazine HCl, inhibit the infection of Delta and Omicron pseudoviruses. Finally, we discovered that these two compounds were highly effective against authentic SARS-CoV-2 viruses, including the WH01, Delta, and Omicron strains. Our study identified potential broad-spectrum SARS-CoV-2 inhibitors and provided insights into the development of novel therapeutics.

简介：雄激素受体(AR)调停了雄激素行动提供在男开发和功能的不仅一个古典枢轴的角色而且在女繁殖生理学的一个最近证明的角色。拼接AR的变体被报导发生在各种各样的雄激素敏感的癌症和现在，由王等的最近的研究。建议AR拼接变体在polycystic卵巢症候群(PCOS)有一个etiological角色。尽管进一步的调查被要求充分估价他们的发现的意义，这些精液的调查结果为在在PCOS的起源和致病发信号的AR的角色的理解打开新章有令人激动、重要的含意。

简介：Romanticismisanimportantstageintheevolutionofman’sself-consciousness.Itshowsnewinterestinandnewconcernwithmanandnature.Wordsworthiswell-knownasanaturepoet.Thethemeofhumanmindandnatureinhispoemshaslongattractedreaders’attention.However,hedoesnotindulgeinnatureitselfbuttakesitasconsolationandinspirationforhumankind.Inordertohighlightthepoet’sconcernwithhumanmind,thepresentpaperchoosestoexploreWordsworth’sconcernwithchildren’smind,basedontheanalysisofWordsworth’sThePreludeandmanyofhispoemsaboutchildren.

简介：切开的修改Hermitian和skew-Hermitian(MHSS)重复方法被黄雾，Benzi和陈介绍并且学习(计算，87(2010)，93-111)为解决复杂对称的线性系统的一个类。在这份报纸，用Toeplitz矩阵的性质，我们为解决复杂Toeplitz建议结构化的MHSS重复方法的一个班线性系统。理论分析证明结构化的MHSS重复方法对准确答案无条件地会聚。当MHSS重复方法直接被使用到复杂对称的Toeplitz线性系统时，计算费用能被Toeplitz结构的使用体谅地减少。最后，数字实验证明结构化的MHSS重复方法和结构化的MHSSpreconditioner为解决复杂Toeplitz是有效的线性系统。[从作者抽象]

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简介：AIM:ToelucidatethemolecularmechanismsunderlyinghepatitisBvirus(HBV)occultinfectionofgenotypeC.METHODS:Atotalof10typesofhepatitisBsurfaceantigen(HBsAg)variantsfromaKoreanoccultcohortwereused.AfteracompleteHBVgenomeplasmidmutatedsuchthatitdoesnotexpressHBsAgandplasmidencoding,eachHBsAgvariantwastransientlyco-transfectedintoHuH-7cells.ThesecretioncapacityandintracellularexpressionoftheHBVvirionsandHBsAgsintheirrespectivevariantswereanalyzedusingreal-timequantitativepolymerasechainreactionassaysandcommercialHBsAgenzyme-linkedimmunosorbentassays,respectively.RESULTS:AllvariantsexhibitedlowerlevelsofHBsAgsecretionintothemediumcomparedwiththewildtype.Inparticular,ineightofthetenvariants,verylowlevelsofHBsAgsecretionthatweresimilartothenegativecontrolweredetected.Incontrast,mostvariants(9/10)exhibitednormalvirionsecretioncapacitiescomparablewith,orevenhigherthan,thewildtype.ThisprovidednewinsightintotheintrinsicnatureofoccultHBVinfection,whichleadstoHBsAgsero-negativenessbuthashorizontalinfectivity.Furthermore,mostvariantsgeneratedhigherreactiveoxidativespeciesproductionthanthewildtype.ThisfindingprovidespotentiallinksbetweenoccultHBVinfectionandliverdiseaseprogression.CONCLUSION:ThepresentlyobtaineddataindicatethatdeficiencyinthesecretioncapacityofHBsAgvariantsmayhaveapivotalfunctionintheoccultinfectionsofHBVgenotypeC.

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简介：Objective:RecentevidenceindicatesthatdysregulationofmicroRNA(miRNA)biogenesisisimplicatedincancerdevelopmentandprogression.BasedontheimportantroleofmiRNAbiogenesisgenesincarcinogenesis,wehypothesizedthatgeneticvariationsofthemiRNAbiogenesisgenesmaymodulatesusceptibilitytocervicalcancer.Methods:Weidentifiedthreesinglenucleotidepolymorphisms(SNPs)locatedinthe3’-untranslatedregions(3’-UTR)ofofmiRNAbiogenesiskeygenes(rs1057035inDICER,rs3803012inRANandrs10773771inHIWI)andgenotypedtheseSNPsinacase-controlstudyof1,486cervicalcancercasesand1,549cancer-freecontrolsinChinesewomen.Results:LogisticregressionanalysesshowedthatnosignificantassociationswereobservedbetweenthethreeSNPsandcervicalcancerrisk[rs3803012inRANAG/GGvs.AAadjustedOR=1.104,95%confidenceinterval(CI):0.859-1.419;rs1057035inDICERCT/CCvs.TTadjustedOR=0.962,95%CI:0.805-1.149;rs10773771inHIWICT/CCvs.TTadjustedOR=0.963,95%CI:0.826-1.122].Conclusions:Thefindingsdidnotsuggestthatgeneticvariantsinthe3’-UTRofRAN,DICERandHIWIofmiRNAbiogenesisgeneswereassociatedwiththeriskofcervicalcancerinthisChinesepopulation.

简介：AbstractSince severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified during late 2019, the sustained spread of this pathogen within the human population has caused worldwide disruption with staggering infection rates and death tolls. Due to the accumulation of mutations in SARS-CoV-2, the virus has evolved into many variants, five of which have been listed as variants of concern VOCs by the World Health Organization (WHO). Multiple animal models of SARS-CoV-2 have been developed to evaluate vaccines and drugs and to assess the pathogenicity, transmissibility and antiviral measures of these VOCs. Here, we review the cutting-edge research based on mouse, hamster, ferret and non-human primate models for evaluating SARS-CoV-2 with a focus on the Omicron variant, and highlight the importance of updating vaccines in a timely manner in order to mitigate the negative effects of SARS-CoV-2 infections in the human population.

简介：AbstractIntroduction:The gap junction beta 6 (GJB6) gene encodes connexin 30. This protein plays critical role in tissues and is responsible for the formation of gap junctions, which have a wide variety of physiological functions. Disease-associated variants of GJB6 cause non-syndromic hearing loss (HL) and skin lesions.We herein describe a Turkish girl who was diagnosed with HL and in whom genetic analysis revealed a causal variant of GJB6.Case presentation:The 1-year-old girl patient was diagnosed with bilateral HL when she was 1 month old. Apart from this, the patient’s motor/mental development and physical examination were normal. As a result of the analysis with the multi-gene panel, the causative genomic change, c.175G>A(p.Gly59Arg) in the GJB6 gene was determined as heterozygous. Segregation analysis proved the same genotype in the patient’s mother and grandfather. The patient’s mother and grandfather had bilateral HL and palmoplantar hyperkeratosis phenotype. The patient was diagnosed with Clouston syndrome, and genetic counseling was provided to her family.Discussion:Causal variants of GJB6 cause skin manifestations and signs of HL. Molecular diagnosis of these patients is a valuable tool for clinicians in reaching their optimal treatment and clinical management.Conclusion:In syndromic cases in which many organs are affected, the determination of the causative gene is important in directing the patients to appropriate observation, screening, and treatment strategies.