简介：Objective:Tomakeaclinicalanalysisonthebasisof36casesofatypicalgenitalherpes(GH)patients.Methods:Thirty-sixcasesofatypicalGHwerediagnosedclinically,andtheircasehistories,symptomsandsignswererecordedindetailandfollowedup.Polymerasechainreaction(PCR)wasadoptedfortestingHSV2-DNAwithcotton-tippedswabs.Enzyme-linkedimmunosorbentassay(ELISA)forserumanti-HSV2-IgMwasdonetoestablishadefinfiivediagnosis.Otherdiagnoseswereexcludedatthesametimebytestingforrelatedpathogensincludingfungi,Chlamydia,Mycoplasma,Treponemapallidum,gonococci,Trichomonas,etc.Results:ThemainclinicalmanifestationsofatypicalGHwere:(1)smallgenitalulcers;(2)inflammationofurethralmeatus;(3)nonspecificgenitalerythema;(4)papuloidnodulesontheglands;(5)nonspecificvaginitis.Twenty-threecases(64%)testedbyPCRwereHSV2-DNAsera-positive,and36cases(100%)anti-HSV2-IgMsera-positivebyELISA.Conclusion:atypicalHSVisdifficulttobediagnosed.ButthecombinationofPCRandELIASwillbehelpfultothediagnosisofatypicalHSV.

简介：Objective:ThisstudyexaminedHerpesSimplexVirus(HSV)subclinicalsheddinginthegenitaltractofpatientswithgenitalherpes(GH)ornon-gonoccalurethritis(NGU).Method:SwabswerecollectedafterexposuretorashandgenitaltractduringGHrelapseorremissiononaweeklybasisforfourtosixweeks.NGUpatientswithnegativechlamydiaandmycoplasmatestswerealsoswabbedforasimilarduration.AllswabsunderwentHSVDNAdetectionwithquantitativePCR.Result:TherewasasignificantdifferenceintherateofasymptomaticHSVsheddinginurinarytractscomparingGHandthecontrolgroupandcomparingNGUandthecontrolgroup(P<0.05).TherateofHSVsheddingwas22%,9.8%and3.3%forGH,NGUandcontrolgroupsrespectively.TherateofHSVsheddingwas21.7%(20/92)forpatientswithactiveGHand23%forthoseinremission.TheHSVpositiveratewassignificantlyhigherinthegroupwithpatientswhohadmorethansixrelapseswithinoneyearcomparedtothegroupofpatientswithlessthansixGHrelapses.Conclusion:ThereisHSVsubclinicalsheddingintheirgenitaltractduringactiveGHandremission.SubclinicalHSVsheddingismorecommoninpatientswithmorethansixGHrelapsesperyearcomparedtoGHpatientswithfewerrelapses.Approximately9.9%ofNGUpatientswithnegativechlamydia,mycoplasmatestingwasfoundtohavesubclinicalHSVinfection.

简介：ClinicalDataInthisgroup,totally17caseswereobserved,including10malesand7females,withtheagerangingfrom18to67yearsoldandwiththedurationfrom2daysto1year.

简介：Theauthortreated16casesofsequelaeofneuralgiaafterherpeszosterwithacupuncture,moxibustionandpointinjectionintheaffectedregion,andallcaseswerecured.

简介：Objective:Todetectandquantitategenitalherpessimplexvirus(HSV)DNAinspecimensfrom100patientsclinicallydiagnosedwithgenitalherpes.Methods:PolymeraseChainReaction(PCR)andenzyme-linkedimmunosorbentassay(ELISA)wereusedwithastandardcurveofDNAcopiesofHSVasquantitativecontrast.Results:Ninety-threecaseswereconfirmedHSVpositiveand7caseswerefoundtobenegative.Therewere58casesofHSV-2(62.4%)and35casesofHSV-1(37.6%)amongthe93positivecases.ThenumberofDNAplasmidsrangedfrom115to1.1×l0^5per250pLamongthe93positivesamples(mean=7.1×10^4/250μL).ThenumberofHSVDNAplasmidsrangedfrom136to1.1×l0^5copiesper250pL(mean=7.6×10^4)amongthosewithHSV-2,and115to9.4×10^4per250pL(mean=6.3×10^4)amongthosewithHSV-1.Meanwhile10μLofextractedanddissolvedDNArandomlytakenfrom8eachofHSV-2andHSV-1samplesweretested.ThenumberofHSV-2DNAplasmidsrangedfrom35copiesto2.7×10^4(Mean=l.8×10^4)andthenumberofHSV-1DNArangedfrom29to2.5×10^4(Mean=1.6×10^4).Inthe7negativecases,thequantityofHSVplasmidswaszero.Conclusion:ThesensitivityofELISAquantitation(93%)isequaltothatofSouthernblot.ThesensitivityofPCRfordiagnosisis91%,and88%forPCRtyping.

简介：Herpeszosterisanacutedermatosiscausedbychlckenpoxvirusinflammationandmarkedmainlybyviolentpainandcord-likeherpesontheskinsurface.IntraditionalChinesemedicine,itistermedas"SheChuanChuang"(蛇串疮).Theintolerablepainexitsinthewholeprocessofthediseaseevenafewweeksormonthsafterdisappearanceoftheherpes,causingthepatienttosufferalot.FromAprilof1997toJuneof2002,theauthoradoptedacupoint-injectionandacupuncturetherapiestotreat19casesofherpeszosterandachievedsatisfactoryresults,hereisthereport.

简介：Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.

简介：Thetherapeuticeffectofherpessimplexvirusthymidinekinase/ganciclovir(HSV-tk/GCV)systemonhepatocellularcarcinomawasstudiedinthisexperiment.Thetk-containingretroviralrecombinantswereusedtoinfecthepatomacells(BEL-7402)andthecellsweretreatedwithganciclovir(0-100μg/ml).TheresultsshowedthatHSV-tkgenecouldbeefficientlytransferredinvitrointohepatomacellsandstablyexpressed.Thegrowthpotentialofthetk-containingcellswassignificantlyinhibitedbyGCV(P<0.01)ascomparedtothenon-tk-containingcells.TheantitumoreffectofHSV-tk/GCVsystemwasalsoproducedexvivointk-containingtumorofnudemiceascharacterizedbyamarkeddecreaseintumorgrowthafterGCVtreatmentcontrarytoaprogressiveenlargementofnon-tk-containingtumors.Althoughthehistologicalexaminationdemonstratedthattheefficiencyofthegenetransferwaslessthan30%,thekillingeffectofHSV-tk/GCVsystemonhepatocellularcarcinomawasstillsignificantlygenerated.ThegropermechanismofHSV-tkgenetherapyonhepatictumorreferredas“bystandereffect”intherapeuticapproachhasnotbeenfoundinthisstudyandrequiredtobeexploredfurther.

简介：

简介：Objective:ThisstudyanalyzedtheTlymphocytesandThl/Th2typecytokineprofileshiftintheperipheralbloodofpatientswithrecurrentgenitalherpes(RGH).Methods:Immunofluorescentstainingofcellsurfaceantigenandintracellularcytokines(IL-2,IL-4,IL-12,IFN-r)inperipheralbloodfrom20RGHpatientsand10controlswereanalyzedusingflowcytometrictechniques.Results:RGHpatientshadsignificantlylowerlevelsofCD3^+Tcells,CD4^+TcellsandCD4^+T/CD8^+Tcellsratiocomparedtocontrollevels(P<0.001),andIL-2-producing,IFN-r-producingandIL-12-producingTcellswereincreasedinRGHpatients(CD4^+T:P<0.001,CD8^+T:P<0.05respectively),whereasIL-4-producingTcellswereincreasedinRGHpatientscomparedtocontrols(CD4+T:P<0.05;CDS^+T:P<0.001respectively).Conclusions:RGHpatientshaveTlymphocytesubsetvariationsandThl/Th2cytokinechanges.TheincreaseinTh2cellsThl/Th2imbalancemayhaveimportantimplicationsforRGHpathogenesis.

简介：ToobservetheeffectofGardeniaextractZGontheadsorptionquantityofherpessimplexvirustype1(HSV-1)soastoexplorethemechanismofitsantiviralactivity,fluoresceinisothiocyanate(FITC)wasusedasthefluorescentprobetolabelvirusesandheparinsodiumwasusedascontrol.Meanwhile,theeffectofGardeniaextractZGontheadsorptionquantityonthesurfaceofHep-2cellswasdeterminedbyflowcytometry.ItwasdemonstratedthatadsorptionofHSV-1onthesurfaceofHep-2cellsexhibitedthecharacterofsaturationandspecificityandheparinsodiumcouldpreventattachmentofvirusesonthesecells.Theseresultsareinaccordwiththosereportedpreviously.Itwasalsoprovedthatthemannerofdrug-usepriortoadsorptionorsimultaneoususeofdrugandadsorptionwasbetterthanadsorptionpriortodrug-use,andtheinhibitionratesoftheformerandlattermannerwere84.76%and82.92%respectively.Threemannersofdrug-usewithGardeniaextractZGwerealleffectivetoreducetheadsorptionquantityofviruses,especiallythemannerofsimultaneoususeofdrugandadsorptionwithanadsorptioninhibitionrateof68.46%.Fromtheaboveobservation,itisapparentthatthemechanismofanti-viralactivityofGardeniaextractZGmaybeviaseveralstepsinvolvedintheHSV-1adsorption.

简介：Objective:TostudytheeffectoftraditionalChinesemedicineantiviralcapsulesinthetreatmentofgenitalherpes.Methods:Usingfemaleguineapiggenitalherpesastheanimalmodel,thisstudyusedoraladministrationoftwoformulationsofantiviralcapsules(AC)andobservedtheeffectonvaginalHSV-2titersandvulvarsymptoms.CellcultureswerealsousedtoexaminethedirectinactivationofHSV-2bytheantiviralcapsulesandthesuppressionofHSV-2viathreedrugadministrationmethods.Results:Therewasnosignificantdifferenceofmeanvaginalvirustitersbetweentheantiviralcapsulegroupsandthatofthepositiveacyclovir(ACV)control(P>0.05).Meanvulvarsymptomscoresofthetwoantiviralcapsulegroupswerealsosignificantlylowerthanthatofthesalinenegativecontrolgroupondays2,3,5,7and8(P<0.05)andsimilartothatoftheACVcontrol(P>0.05).CellcultureshowedtheminimuminhibitoryconcentrationsofantiviralcapsulesNo.1andNo.2were0.390625mg/mland1.5625mg/ml,respectively.Conclusion:ThetraditionalChinesemedicineantiviralcapsuleshadsuppressiveeffectsonHSV-2inbothanimalmodelGHandinvitrocellculture.

简介：Sixty-fivecasesofherpeszosterwererandomlydividedintotheHe-Nelasergroup(33cases)andpolyinosinicacidgroup(32cases).TypeJIHe-NelaseruniversaltreatmentapparatuswasusedtoirradiateinjuredcutaneousregionsandtoradiateHegu(LI4)andZusanli(ST36)pointswithphotoconductivefibersonceeverydayintheHe-Nelasergroup;2mlofpolyinosinicacidwasin-tramuscularlyinjectedonceeveryotherdayintheothergroup.Ofthe65cases63werecured,andthetwocaseswithsequelaofneuralgiawereallinthelattergroup.Painwasdisappearedandscabswereformedrespectivelyafter1.48and5.76daysoftreatmentwithHe-Nelasertherapy,andafter10.5and10.4dayswithpolyinosinicacidtreatment.Thedifferencesoftherapeuticeffectsbetweenthetwogroupswerenoticeablysignificant(P<0.01).

简介：TostudytheroleofTh1/Th2cytokinesinthepathogenesisofrecurrentgenitalherpes(RGH),andtobetterunderstandtherelationshipbetweenthem.Methods:Atwo-colorimmunofluorescentstainingofcellsurfaceantigenandintracellularcytokinesforflowcytometricanalysiswasusedforCD3,IL-2,IL-10,IL-12,IFN-yandTNF-ainCD3^+T-lymphocytesinactivatedperipheralbloodmononuclearcellsofpatientswithRGH.Results:Comparedtocontrols,patientswithRGHshowedfewerCD3^+Tcells(P<0.05)andIL-2producingandIFN-7producingTcells(P<0.02andP<0.001,respectively)afterinvitrostimulationwithPMAandionomycininthepresenceofaproteintransportinhibitor.MoreIL-10producingandIL-12producingTcellswerefoundinpatientswithRGH(P<0.01).TherewasnosignificantdifferenceinthenumberofTNY-αproducingcellsbetweenRGHpatientsandcontrols(P<0.05).Conclusion:RGHpatientsshowedrelativelymoreTh2cytokines.TheimbalancebetweenThlandTh2cytokinesresultsininhibitoryeffectsonaseriesofcell-immuneresponses,whichmayplayanimportantroleinthepathogenesisofRGH.

简介：Objective:Tostudytheroleofmonocytesinthepathogenesisofgenitalherpes.Methods:TNF-aandIL-6levelsin27casesofgenitalherpesweredetectedbyenzymelinkedimmunosorbantassay(ELISA).HLAclassⅡantigenexpressiononmonocytesweredetectedbyanalkalinephosphataseanti-alkalinephosphatasemethod.Results:Comparedwithnormalcontrols,levelsofTNF-aandIL-6secretedbymonocytesrespondingtoLPSmitogeninvitroweresignificantlydecreased[(3.13±0.44ng/ml)vs(4.68±0.54ng/ml),P<0.05and(3.32±1.06ng/ml)vs(6.46±1.94ng/ml),P<0.05,respectively].HLAclassⅡantigenexpressiononmonocytesinthegenitalherpesgroupwasalsosignificantlydecreased[HLA-DR(67.48%±1.51%)vs(81.03%±1.32%),P<0.01andHLA-DQ(29.54%±1.15%)vs(37.63%±1.79%),P<0.01respectively].Conclusion:Thesefindingssuggestthatthedecreasedmonocytefunctionmaycontributetothepathogenesisofgenitalherpes.Augmentingorinducingmonocytefunctionmaybeimportantintheprevention,treatment,andreductionofgenitalherpescases.

简介：TherearesomeIndicationsthttherpesaimplexvirus(HSV)maybemutagenic.SpecificchromosomalchangeshavealsobeendemonstratedInculturedcellsinfectedwithHSV.TofurtherInvestigatethemutagenicactivityofHSVtype2(HSV-2)weusedmouseskinasamodelsystemforcardnogenetls.Inoculationofthebackskinof4-week-oldSencarmkewithlivevirustwiceperweekforoneweekorwithInactivatedvirustwiceperweekfortwoweekswasusedtoInitiatethemouseakin.AfterInitiationwithHSV-2,12-O-tetradecanoylphorbol-13-acetale(TPA)wasappliedtwiceweeklyfor50weeksasapromoter.Duringaperiodof52weeks,noskincarcinomawasfoundIntheexperimentalgroups,whereas55%ofcontrolmicetreatedwith9,10-dlmethy1-1,2-benzanthracene(DMBA)andthenwithTPA-developedskincarcinoma.TheresultsdemonstratethatHSV-2couldnotsubstituteforDMBAinthisanimalmodeloftwo-stageskincarcinogenesis.

简介：Objective:ToevaluatetheclinicalapplicationofmultiplexPCRinthedetectionofTreponemapallidum,Herpessimplexvirus(HSV),andHaemophilusducreyi.Method:ThreestandardstrainswereusedtosetupamultiplexPCR(MPCR)fordetectingsyphilis,herpesgenitalis,andchancroidsimultaneously.Samplesfrom122patientswithgenitalulcerdisease(GUD)weresubjectedtoMPCRandtheresultswerecomparedwiththeseofdark-fiddmicroscopyandTPserology,HSVanligenELISA,andH.ducreyiculture,Result:Inthe122patientswithGUD,MPCRidentified34casesofT.palliduminfection,40casesofHSVinfection,and2casesofmixedinfectionofT.pallidumandherpes.NopositiveresultsofH.ducreyiwerefound.ThesensitivityofMPCRtoT.pallidumandherpeswas100%and93.3%,respectivdy.Thesensitivitiesofdark-fieldmicroscopyandTPserology,HSVantigenELISA,andH.ducreyiculturewas35.3%,50%and100%,respectively.Conclusion:MPCRshowedarelativelyhighersensitivityforT.pallidumascomparedwiththeroutinetechniques.AlthoughitssensitivityforHSVwasnotasgoodasthatofantigenELISA,italsoyieldedahighdetectionrate.MPCRcandetectmorethanonepathogen.Itissimple,quick,sensitive,andsuitableforclinicaluseorepidemiologicalinvestigation.