简介:ToanalyzethegenomicmolecularstructureandgenotypeofhumanastrovirusisolatedfrominfantinGuangzhouofChina,theprimersweredesignedbasedonthegenomicsequenceofastrovirusfromtheGenBankandthetargetsequencewereamplifiedbyRT-PCR.ThenthePCR-productswereclonedtoTvectorandsequenced.ThegenomicnucleotidesequenceswereanalyzedbytheprogramsCLUSTALWandDNASTAR.ItwasfoundthatthefullgenomiclengthofHASTVgz01strainwas6721bpandtheORFswere6558bp.The5'and3'UTRwere82and81nucleotides.Thegenomeincluded3openreadingframes(ORFs):ORF1a,ORF1bandORF2.The5'-terminalORF1astartedatnucleotide83andextendedtonucleotide2845.ORFlb(nt2785tont4332)overlapedORFlaby61nucleotides.The3'-terminalORF2beganatnucleotide4325andterminatedatnucleotide6640.ORF2had2316nucleotides.ComparedwithotherastrovirussequencesinGenBank,thehomologyoftheaminoacidsequenceofORF2ofHASTVgz01strainwiththatofserotype4was93%.Homologywithotherserotypesrangedfrom61%to70%.ThecompletenucleotidesequenceofastrovirusHASTVgz01strainisolatedfromGuangzhouinChinawas6721bpinlength,GenBankaccessionNO.DQ344027.ComparingtheORF2ofastrovirusHASTVgz01withtheknownsequencesoftypes1-8thehighesthomologywasserotype4(93%).ComparativesequenceanalysisoftheHASTVgz01ORF2withthereportedhumanastrovirussequencesrevealedthattheisolatedastrovirusbelongstogenotype(serotype)4.
简介:TheGroupofEnergyMaterials(GEM)inIMPisengagedintheirradiationresponseofmaterialscandidatetoadvancednuclearenergysystems(GenIV,fusionreactors).Themajorprogressofresearchinourgroupin2016isintheirradiationhardening/embrittlementofoxide-dispersion-strengthened(ODS)ferriticsteelsandVanadiumalloys,andinthemechanismsunderlyingdamageproductioninsiliconcarbide(SiC)fibers.Abriefdescriptionisgivenasfollows.1.EffectoftheoxidenanoparticlesontheirradiationhardeningofODSferriticsteelsTheinfluenceofoxidenanoparticlesontheirradiationresistanceofODSferriticsteelsisacrucialissuefortheupgradeofODSsteels.
简介:Asafundamentalpropertyofnuclei,atomicmassesarewidelyusedinmanydomainsofscienceandengineering.Areliableatomicmasstablederivedfromtheexperimentaldata,wheretheatomicmassesandtherelevantexperi-mentalinformationcanbefoundconveniently,isinhighdemandbytheresearchcommunity.Tomeetthedemands,theAtomicMassEvaluation(AME)wasinitiatedin1950'sandaseriesofAMEmasstableshavebeenpublishedeversince.CurrentlytheAMEservestheresearchcommunitybyprovidingthemostreliableandcomprehensiveinformationrelatedtotheatomicmasses.ThenewatomicmassevaluationAME2016waspublishedintheMarchissueofChinesePhysicsCastwocom-plementarypapers[1;2].
简介:Themainworkoftheirradiationtechniquegroupfocusesonthefollowingtwoaspectsin2016:1.TechniquesupportWehavehadabout772.5hbeamtimefor39roundsofsingleeventeffect(SEE)tests,whichiscooperatingwithvariousinstitutes,universities,andcompanies.Thetestsarethebasicsafeguardforthespacecraftandsatellite.InordertoguaranteethetestefficiencyoftheTerminal5,themaintenanceandimprovementforthevacuum,thesampletransferandbeammonitorsystemhavebeenachieved.
简介:1.ThemutationbreedingofplantsIn2016,themutationmechanismofvariousplantmutantsincludingArabidopsisthaliana,Geranium,Trades-cantiauminensis,TaraxacumkoksaghyzRodinandalgaeobtainedbyheavyionbeammutationbreedingtechniqueswereinvestigated.Thegenome-wideprofilingofanArabidopsismutantnamedcivar(CarbonionbeamsInducedVariegated)wasrevealedbyusingre-sequencingandtheroughmap-basedcloningtechniques.AphenotypescreeningofArabidopsisthalianairradiatedbycarbonionbeamsbasedonhigh-throughputimagingtechniquecombiningtheprincipalcomponentanalysisandscattermatrixclusteringanalysiswasbuiltandoptimizedwhichcanscreenthevariationofplantmutantpopulations.Thepigmentcomponentsandquantitiesandexpressionprofilesofkeygenesinvolvedinanthocyaninbiosynthesisofflower-colormutantofGeraniumandleaf-colormutantofTrades-cantiauminensiswereinvestigated.MutationbreedingofTaraxacumkoksaghyzRodininducedbycarbonionbeamirradiationswhichisnotableforitsproductionofhighqualityrubberandinulinwasstarted.Inaddition,thephotosyntheticresponseofthemutantsofScenedesmusquadricaudawithalteredpigmentfeatureswereanalyzed.
简介:由于工程设计在电路板设计方面对使用更小的电子封装方面越来越有兴趣,0201元件对PCB(印刷电路板)装配者已变成了争相采用的元件。这样一来,在0201元件应用到电路板设计以前,一些领域如电路板设计,装配工艺,检测方法,修理工艺,焊点可靠性必须重新评估。这篇文章从一个0201工程的评估工作中提供一些结论和建议。设计一种0201测试板用以研究可制造性设计(DFM)规则,关键组装参数检验方法、修理工艺和焊点可靠性。这篇文章介绍了一些用未评估这方面的方法。为研究模板设计和印刷工艺的关键参数,设计了用于模板印刷工艺的实验(DOE)。对于焊盘研发了评估贴放工艺窗口的方法。更宽的工艺窗口可以通过焊盘优化实现。在进一步对比中,研究了AOI(自动光学检测),X射线和视频显微镜检测。在检测方法的选择上,考虑投资成本,人力要求及筛漏效率。另外,评估了修理工具和方法。最后,用振动和热循环检测了焊点的可靠性。