简介:ToanalyzethegenomicmolecularstructureandgenotypeofhumanastrovirusisolatedfrominfantinGuangzhouofChina,theprimersweredesignedbasedonthegenomicsequenceofastrovirusfromtheGenBankandthetargetsequencewereamplifiedbyRT-PCR.ThenthePCR-productswereclonedtoTvectorandsequenced.ThegenomicnucleotidesequenceswereanalyzedbytheprogramsCLUSTALWandDNASTAR.ItwasfoundthatthefullgenomiclengthofHASTVgz01strainwas6721bpandtheORFswere6558bp.The5'and3'UTRwere82and81nucleotides.Thegenomeincluded3openreadingframes(ORFs):ORF1a,ORF1bandORF2.The5'-terminalORF1astartedatnucleotide83andextendedtonucleotide2845.ORFlb(nt2785tont4332)overlapedORFlaby61nucleotides.The3'-terminalORF2beganatnucleotide4325andterminatedatnucleotide6640.ORF2had2316nucleotides.ComparedwithotherastrovirussequencesinGenBank,thehomologyoftheaminoacidsequenceofORF2ofHASTVgz01strainwiththatofserotype4was93%.Homologywithotherserotypesrangedfrom61%to70%.ThecompletenucleotidesequenceofastrovirusHASTVgz01strainisolatedfromGuangzhouinChinawas6721bpinlength,GenBankaccessionNO.DQ344027.ComparingtheORF2ofastrovirusHASTVgz01withtheknownsequencesoftypes1-8thehighesthomologywasserotype4(93%).ComparativesequenceanalysisoftheHASTVgz01ORF2withthereportedhumanastrovirussequencesrevealedthattheisolatedastrovirusbelongstogenotype(serotype)4.
简介:TheGroupofEnergyMaterials(GEM)inIMPisengagedintheirradiationresponseofmaterialscandidatetoadvancednuclearenergysystems(GenIV,fusionreactors).Themajorprogressofresearchinourgroupin2016isintheirradiationhardening/embrittlementofoxide-dispersion-strengthened(ODS)ferriticsteelsandVanadiumalloys,andinthemechanismsunderlyingdamageproductioninsiliconcarbide(SiC)fibers.Abriefdescriptionisgivenasfollows.1.EffectoftheoxidenanoparticlesontheirradiationhardeningofODSferriticsteelsTheinfluenceofoxidenanoparticlesontheirradiationresistanceofODSferriticsteelsisacrucialissuefortheupgradeofODSsteels.
简介:Asafundamentalpropertyofnuclei,atomicmassesarewidelyusedinmanydomainsofscienceandengineering.Areliableatomicmasstablederivedfromtheexperimentaldata,wheretheatomicmassesandtherelevantexperi-mentalinformationcanbefoundconveniently,isinhighdemandbytheresearchcommunity.Tomeetthedemands,theAtomicMassEvaluation(AME)wasinitiatedin1950'sandaseriesofAMEmasstableshavebeenpublishedeversince.CurrentlytheAMEservestheresearchcommunitybyprovidingthemostreliableandcomprehensiveinformationrelatedtotheatomicmasses.ThenewatomicmassevaluationAME2016waspublishedintheMarchissueofChinesePhysicsCastwocom-plementarypapers[1;2].
简介:Themainworkoftheirradiationtechniquegroupfocusesonthefollowingtwoaspectsin2016:1.TechniquesupportWehavehadabout772.5hbeamtimefor39roundsofsingleeventeffect(SEE)tests,whichiscooperatingwithvariousinstitutes,universities,andcompanies.Thetestsarethebasicsafeguardforthespacecraftandsatellite.InordertoguaranteethetestefficiencyoftheTerminal5,themaintenanceandimprovementforthevacuum,thesampletransferandbeammonitorsystemhavebeenachieved.
简介:1.ThemutationbreedingofplantsIn2016,themutationmechanismofvariousplantmutantsincludingArabidopsisthaliana,Geranium,Trades-cantiauminensis,TaraxacumkoksaghyzRodinandalgaeobtainedbyheavyionbeammutationbreedingtechniqueswereinvestigated.Thegenome-wideprofilingofanArabidopsismutantnamedcivar(CarbonionbeamsInducedVariegated)wasrevealedbyusingre-sequencingandtheroughmap-basedcloningtechniques.AphenotypescreeningofArabidopsisthalianairradiatedbycarbonionbeamsbasedonhigh-throughputimagingtechniquecombiningtheprincipalcomponentanalysisandscattermatrixclusteringanalysiswasbuiltandoptimizedwhichcanscreenthevariationofplantmutantpopulations.Thepigmentcomponentsandquantitiesandexpressionprofilesofkeygenesinvolvedinanthocyaninbiosynthesisofflower-colormutantofGeraniumandleaf-colormutantofTrades-cantiauminensiswereinvestigated.MutationbreedingofTaraxacumkoksaghyzRodininducedbycarbonionbeamirradiationswhichisnotableforitsproductionofhighqualityrubberandinulinwasstarted.Inaddition,thephotosyntheticresponseofthemutantsofScenedesmusquadricaudawithalteredpigmentfeatureswereanalyzed.