简介：Thedistributionofrareearthelement(REE)formsinsoilsisgiveninthepresentpaperonthebasisofsequentialfractionationanddeterminationof34representativesurfacesoilscollectedfromallpartsofChina.ResultsobtainedshowthattheextractionrateofwatersolubleandexchangeableREEhadtheodd-evenphenomenonandthatoforganicallyboundREEfromLatoLutendedtoreducegraduallywiththeincreaseofatomicnumber.ThestabilityofScandtheactivityandenrichmentofTminsoilsareproposed.Furthermore,thepapermakesapreliminarydiscussiononthedifferencesintheconcentrationdistributionofvariousformsofindividualREEinsomesoils.

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简介：Chinesefir(Cunninghamialanceolata)andpoplar(Populusspp.)woodmealwerephenolatedinthepresenceofsulfuricacidusedasacatalyst.Theeffectsofreactiontimeandreactiontemperatureonthewoodliquefactionwereinvestigated.Theresultsshowedthatthereactiontemperaturehadthegreaterinfluenceontheresiduecontentthanreactiontime.Additionally,theliquefactioncurvefortheChinesefirandPoplarweresimilaringeneral.

简介：为建立鸭瘟病毒（DPV）快速、敏感的检测方法,本研究利用PCR技术扩增出DPVUL30基因中510bp的保守序列,并克隆到pMD18-T载体中作为标准品制作标准曲线,建立了DPV的SYBRGreenI荧光定量PCR检测方法。该方法检测灵敏度可达5×101拷贝,与鸭细小病毒、鸭圆环病毒、小鹅瘟病毒、鸭肝炎病毒、鸭H9亚型流感病毒和鸭副粘病毒均不发生交叉反应,具有良好的特异性和重复性。结果表明,建立的实时荧光定量PCR具有特异、敏感、快速、定量、重复性好等优点,可用于临床DPV的检测。

简介：Adsorptionanddesorptionofexogenousrareearthelements(REE)insoilswerestudied.ResultsshowedthatsoilshadstrongadsorbabilityforREEandtherateofadsorptionofREEwasover95%oftheaddedREEinthesetests.ThecharacteristicsofadsorptionisothermscorrespondedwellwiththebothFreundlichandTemkinequations,butdeviatedfromtheLangmuirequation.TheadsorptionofREEtendedtoincreasewiththerisingofsoilpH.AsequentialextractionmethodusedforstudingthedesorptionanddistributionofREEsorbedinsoilsarealsodiscussed.

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简介：Endophyticfungiarewidelyfoundinalmostallkindsofplants.Manyendophyticfungicanproducesomephysiologicalactivecompounds,whicharesametooranalogtothoseisolatedfromtheirhosts.Producingphysiologicalactivecompoundsthroughmicrobialfermentationcangiveanewwaytoresolveresourcelimitationandtofindoutalternativesource.Throughthemethodsoforganicsolventextraction,thinlayerchromatography(TLC)andcolumnchromatography,compoundIwasisolated,purifiedfromtheliquidfermentationmetabolitesofthetaxoids-producedendophyticfungi(Alternaria.alternatavar.taxi1011Y.XiangetLUAn-guo)thatwasscreenedfromthebarkofTaxus.cuspidataSieb.etZucc..CompoundIwasidentifiedasonekindoftaxoidstypeIII,basedontheanalyzingresultsbyusingthemethodsofultravioletspectroscopy(UV),infraredspectroscopy(IR),massspectrometry(MS)andnuclearmagneticresonancespectroscopy(NMR).Thisstudyprovidesacompletedmethodforseparationandpurificationoftheendophyticfungiaswellasstructureidentificationofitsfermentationmetabolite

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简介：Ｉ－６９杨插穗分别培养在含有不同浓度Ｃｅ（ＮＯ３）３的营养液中，经快速冷冻、冷冻干燥、无水塑料包埋及干刀薄切片法，用Ｘ－射线能谱微区分析测定Ｃｅ及其它无机离子在茎尖分生组织和叶的不同组织中细胞壁、细胞质、细胞核及叶绿体中的含量及分布。在１０ｐｐｍＣｅ（ＮＯ３）３处理下，茎尖中Ｃｅ的含量比对照组和１００ｐｐｍＣｅ（ＮＯ３）３处理组高出近１倍，叶的亚细胞中的Ｋ、Ｐ、Ｓ、Ｆｅ的含量也呈增加的趋势。特别是Ｍｇ的含量增加显著，在保卫细胞的细胞质中Ｍｇ的含量比对照组高出３．５倍，而在表皮细胞的细胞核和液泡中的含量则比对照组高出近１３倍。但在１００ｐｐｍＣｅ（ＮＯ３）３处理下，无机离子在叶中亚细胞微区间的含量则显著下降