简介:摘要目的探讨光线对血清胆红素测定结果的影响。方法收集30例黄疸患儿的血清标本,每份标本都分成甲组和乙组,即刻离心测定血清总胆红素后,将甲组置于室温下不避光,乙组置于室温下避光,待2h、4h、8h及24h再次测定血清中的胆红素含量。结果置于室温下不避光组2h、4h、8h及24h与采血后即刻所测血清胆红素结果比较,下降率分别为10.54%、19.75%、27.61%和37.78%;室温下避光组2h、4h、8h及24h与采血后即刻所测血清胆红素结果比较,下降率分别为0.17%、0.64%、1.23%和1.50%。结论自然光线照射后各时相所测血清胆红素值水平明显降低,而避光保存后各时相所测得血清胆红素值水平下降不明显。因此,如当天不能及时测定的标本应该避光保存。
简介:摘要:目的:分析改良式温箱罩周期变化的光线对早产儿生长发育的影响。方法:将我院在2019年01月至2022年01月时间段内收治的60例早产儿为样本,按照摸球法的原则分为对照组和研究组,每组30例,前者应用常规护理,后者联合改良式温箱罩,观察对照组和研究组经不同方式护理后的效果,对比两组进奶量及体重。结果:两组早产儿在入院时进奶量及体重比较,数据差异性不符合正态分布,P>0.05,研究组在入院15天后进奶量及体重明显高于对照组,组间数据差异性符合正态分布,P<0.05。结论:早产儿应用改良式温箱罩周期变化的光线,有利于改善其消化能力,提高进奶量,促进其生长发育,建议临床医学推广。
简介:摘要目的探讨一氧化氮(NO)供体和聚多巴胺复合纳米体系的制备方法以及其对肝癌细胞的抑制作用。方法采用化学合成法制备聚多巴胺(PDA)负载一氧化氮供体S-亚硝基半胱胺衍生物(SNO)得到纳米载药复合体系SNO@PDA,通过磁共振成像和质谱分析进行化学结构鉴定,通过动态光散射技术和透射电镜检测其粒径和形貌表征,分别通过电子温度计和Griess试剂盒检测光热稳定性以及NO的释放。最后通过噻唑蓝(MTT)实验检测纳米载药复合体系对肝癌细胞株Huh7的生长抑制作用。两组间均数比较用t检验,多组间均数比较用单因素方差分析。结果磁共振成像和质谱分析确认成功合成SNO,动态光散射技术测定SNO@PDA的粒径在190 nm左右,透射电镜显示为球形颗粒,形态均一,且在生理条件下可稳定存在。SNO@PDA的载药率为19.4%,包封率为71.4%。当浓度为35 mg/L时,近红外(Near-infrared,NIR)照射后温度可升高12 ℃左右。重复4个开/关循环后升温幅度没有变化。3个循环后NO的释放率达到86%。MTT法测定细胞存活率,当浓度为60 mg/L时,SNO@PDA+NIR组的细胞存活率为(37.2±2.2)%,低于SNO+NIR组(60.1±7.2)%,差异有统计学意义(t=6.500,P<0.01);低于空白材料PDA组(73.9±0.5)%,差异有统计学意义(t=5.900,P<0.01);低于PDA+NIR组(57.9±7.1)%,差异有统计学意义(t=6.400,P<0.01)。结论SNO@PDA可以发挥化疗-光热疗法的组合优势,有效抑制肝癌细胞的增殖。
简介:AIM:Toexploretheeffectofsaturatedhydrogensalineonbluelight-inducedretinaldamageinrats.·METHODS:Theretinaldamageofratswasinducedbybluelightexposurefor6hoursandexamined8hours,16hoursand24hoursaftertheexposure.OnehundredfemaleSprague-Dawleyratswererandomlydividedintofourgroups.Group1included30ratsreceivedlightexposurewithoutanyothertreatment.Group2included30ratsreceivedlightexposurewithintraperitonealinjectionofnormalsaline.Group3included30ratsreceivedlightexposurewithintraperitonealinjectionofsaturatedhydrogensaline.AndGroup4includedtheother10ratswhichdidnotreceiveanytreatment.Theamountofintraperitonealinjectionofsaturatedhydrogensalineandnormalsalinewascalculatedintheratioof1ml/100gofratweight.SpecimenswerecollectedandprocessedbyH-Estaining,ultrastructureobservation,biochemicalmeasurement.Morphologicalchangeswereobservedbylightmicroscopeandtransmissionelectronmicroscope(TEM)andtheretinalouternuclearlayer(ONL)thicknesswasmeasuredbyIPP6.0,whilethemalondialdehyde(MDA)wasmeasuredbycolorimetricdeterminationat532nm.·RESULTS:AlthoughthestructureofretinainGroup1andGroup2wasinjuredheavily,theinjuryinGroup3wasmild.ThedifferencesbetweenGroup1andGroup2werenotsignificant.ComparedwiththeratsinGroup1andGroup2,theonesinGroup3hadmoreclearlydemarcatedretinastructureandmoreorderedcellsbylightmicroscopeandTEMobservation.TheONLthicknesses(400times)offourgroupsateachtimepointexceptbetweenGroup1andGroup2weresignificantlydifferent(P<0.05).ThethicknessesoftheONLinGroup1atthreetimepointswere30.41±4.04μm,26.11±2.82μmand20.63±1.06μm,inGroup2were31.62±4.54μm,25.08±3.63μmand19.07±3.86μm,inGroup3were29.75±3.62μm,28.83±1.97μmand27.61±1.83μm.InGroup4themeanofthethicknesswas37.35±1.37μm.Astimewentby,thedamageg
简介:AIM:Anaerobicbacteriacancauseocularinfections.WetestedtheOxyPlateTMAnaerobicSystem(OXY)toisolatepertinentanaerobicbacteriathatcancauseoculardisease.METHODS:OXY,whichdoesnotrequiredirectanaerobicconditions(i.e.bags,jars),wascomparedtoconventionalisolationofincubatingculturemediainanaerobicbags.Standardcoloniescountswereperformedonanaerobicocularbacterialisolatesunderaerobicandanaerobicconditions(anaerobicbags)usingagarmedia:1)OXY(aerobiconly),2)5%sheepblood(SB),3)Chocolate,and4)Schaedler.Thebacteriatestedwerede-identifiedocularisolatesculturedfromendophthalmitisanddacryocystitisthatinclude10Propionibacteriumacnesand3Actinomycesspecies.Thecolonycountsforeachbacteriaisolate,oneachculturingcondition,wererankedfromlargesttosmallest,andnon-parametricallycomparedtodeterminethebestculturingcondition.RESULTS:Allanaerobicconditionswerepositiveforalloftheanaerobicisolates.SBandSchaedler’sagarunderaerobicconditionsdidnotsupportthegrowthofanaerobicbacteria.SparsegrowthwasnotedonchocolateagarwithPropionibacteriumacnes.Asananaerobicsystem,SBinananaerobicbagisolatedhighercolonycountsthanOXY(P=0.0028)andchocolateagar(P=0.0028).CONCLUSION:AlthoughOXYdidnottesttobemoreefficientthanotheranaerobicsystems,itappearstobeareasonablealternativeforisolatinganaerobicbacteriafromocularsites.Theuseofanagarmediuminaspeciallydesignedplate,withouttherequirementofananaerobicbag,renderedOXYasanadvantageoverotheranaerobicsystems.
简介:AIM:TodiscusstheimpactofLyciumBarbarumPolysaccharide(LBP)andDanshensupurifiedfromTraditionalChineseMedicine(TCM)onvascularendothelialgrowthfactor(VEGF)ofrabbitswithretinalneovascularization.METHODS:Fortyrabbitsweredividedintonormalcontrolgroup,modelcontrolgroup,LBPgroupandDanshensugroup.Animalsinthenormalcontrolgroupwerefedinthenormaloxygenenvironment.Animalsintheotherthreegroupswereputintotheenvironmentwith70%oxygenfor5daysinordertobuildthemodelofoxygen-inducedvascularproliferationretinopathy.AndthendifferentTCMextractwasinjectedintotheabdominalcavitiesoftheseannimals.After7days,theVEGFcontentofintheserumofrabbitwasmeasuredbydoubleantibodysandwichmethod.RESULTS:DataanalysisindicatedthatVEGFcontentwasasfollows:Danshensugroupwaslowerthanmodelcontrolgroup(12.92±3.84ng/Lvs19.32±4.15ng/L,P<0.05);LBPgroupandnormalcontrolgroupwerelowerthanmodelcontrolgroup(12.92±3.84ng/L,9.26±1.61ng/Lvs19.32±4.15ng/L,P<0.01);totalbloodviscosity,plasmaviscosity,cholesterolcontent,fibrinogencontentandtriacylglycerolcontentafterperitonealinjectionofLBPandDanshensuwereobviouslylowerthanbeforeinjection.CONCLUSION:TCMextract-LBPandDanshensucanprominentlyreducethecontentofVEGFintheprocessofvascularproliferativeretinopathyofrabbit;canpreventtheoccurrenceofretinalmicrovasculardiseasebyimprovingpartialoxygen-deficientenvironmentoraffectingallkindsofnewgrowthfactor.