简介：Inthisresearch,thedegradationofdibenzothiophene(DBT)wasinvestigatedbyusingPseudomonassp.LKY-5isolatedfromoilcontaminatedsoil.Theresponsesurfacemethodology(RSM)basedontheBox-Behnkendesign(BBD)wasappliedforevaluatingtheinteractiveeffectsoffourindependentvariablesincludingsubstrateconcentration,temperature,pHandagitationrateontheDBTremovalresponse.Atotalof29experimentsforfourfactorsatthreelevelswereconductedinpresentstudy.Asecond-orderregressionmodelwasthendeveloped,andtheanalysisofvariance(ANOVA)illustratedthattheproposedquadraticmodelcouldbeutilizedtonavigatethedesignspace.Thevalueofdeterminationcoefficient(R2=0.9534)indicatedasatisfactoryagreementbetweenthequadraticmodelandtheexperimentaldata.ItwasfoundthatDBTremovalwasmoresignificantlyaffected(P<0.0001)bysubstrateconcentrationcomparedwithotherthreeparameters.An100%degradationofDBTcouldbeobtainedbyPseudomonassp.LKY-5atasubstrateconcentrationof100mg/L.