学科分类
/ 2
40 个结果
  • 简介:Thepromoterregionofadroughtandabscisicacid(ABA)induciblegene,osr40c1,wasisolatedfromasalt-tolerantindicaricevarietyPokkali,whichis670bpupstreamoftheputativetranslationstartcodon.Insilicopromoteranalysisofresultedsequenceshowedthatatleast15typesofputativemotifsweredistributedwithinthesequence,includingtwotypesofcommonpromoterelements,TATAandCAATboxes.Additionally,severalputativecis-acingregulatoryelementswhichmaybeinvolvedinregulationofosr40c1expressionunderdifferentconditionswerefoundinthe5′-upstreamregionofosr40c1.TheseareABA-responsiveelement,light-responsiveelements(ATCT-motif,BoxI,G-box,GT1-motif,Gap-boxandSp1),myeloblastosisoncogeneresponseelement(CCAAT-box),auxinresponsiveelement(TGA-element),gibberellin-responsiveelement(GARE-motif)andfungal-elicitorresponsiveelements(BoxEandBox-W1).Aputativeregulatoryelement,requiredforendosperm-specificpatternofgeneexpressiondesignatedasSkn-1motif,wasalsodetectedinthePokkaliosr40c1promoterregion.Inconclusion,thebioinformaticanalysisofosr40c1promoterregionisolatedfromindicaricevarietyPokkaliledtotheidentificationofseveralimportantstress-responsivecisactingregulatoryelements,andtherefore,theisolatedpromotersequencecouldbeemployedinricegenetictransformationtomediateexpressionofabioticstressinducedgenes.

  • 标签: 启动子序列 计算机分析 籼稻品种 分离 顺式调控元件 CAAT盒
  • 简介:有低glutelin内容的瑞斯作为为肾失败影响的病人的功能的食物合适。在米饭的低glutelin内容基因Lgc1有在二高度类似的glutelin基因GluB4和GluB5之间的3.5-kb删除,它在染色体2的短手臂上定位。在低glutelin内容米饭改进选择效率繁殖,指定为InDel-Lgc1-1和InDel-Lgc1-2的二分子的标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二标记的联合使用能容易把Lgc1的遗传型与不同米饭变化区分开来。作为一种简单、便宜的技术,因此,分子的标记能广泛地被用来与Lgc1基因识别不同变化并且在低glutelin内容米饭的帮助标记的选择适用。

  • 标签: 蛋白基因 水稻育种 分子标记 蛋白含量 ORYZA PCR检测
  • 简介:Twostarch-branchingenzyme(SBE)inrice,isknowntobeakeyenzymeinamylopectinbiosynthesis.ThecDNAoftwoSBE(starch-branchingenzyme)genesSheIandShedencodingSBEⅠandSBEⅢ(twomajorisoformsinrice)wereclonedbyanimprovedRT-PCRtechnique,fromatemplatecDNAlibray,derivedfromthetotalmRNAsextractedfromtheimmatureseedsofajaponicariceWuyunjing7.DNAsequenceanalysisshowedthatthesizeoftheclonedSheIandShedcDNAswere2490and2481bplong,respectively,includingtheirentirecodingsequences.ComparisonanalysisindicatedthatthenucleotidesequenceofShe3wasthesameasthatofshed(GenbankAccessionNo.D16201)asreportedpreviously.Therewereonlyfourbase-pairsdifference,whichresultedinchangesoftwodeducedaminoacidsbetweentheclonedShe1cDNAandthereportedshe1(GenbankAccessionNo.D11082).TheclonedSheIandShedcDNAsmakeitpossibletoimprovericestarchqualitythroughgeneticengineering.

  • 标签: 水稻 淀粉分支酶 基因 克隆 序列分析 CDNA
  • 简介:Lipoxygenase3(LOX3)isamajorcomponentoftheLOXisozymesinmaturericeseeds.ToinvestigatetheroleofLOX3geneunderstresses,aplantexpressionvectorcontainingantisensecDNAofLOX3wasconstructed.RicevarietiesWuyunjing7andKasalathweretransformedbytheAgrobacterium-mediatedmethodandtransgenicriceplantsweregenerated.PCRandSouthernblotresultsshowedthattheantisenseLOX3genewasintegratedintothericegenome.AnalysesofembryoLOX3deletionandsemi-quantitativeRT-PCRconfirmedtheantisensesuppressionofLOX3geneintransgenicplants.TheT2antisenseplantsofLOX3weresensitivetodroughtstress,riceblastandbacterialblightcomparedwithnon-transgenicplants.TheseresultssuggestthattheLOX3genemightfunctioninresponsetostresses.

  • 标签: RICE LIPOXYGENASE GENE antisense plant transgenic
  • 简介:TounderstandthewildOryzagenomeeffectonphotosynthesisanditsrelationtototaldrymatteraccumulationinanelitericevariety,asetof40stableintrogressionlines(ILs)BC3F8derivedfromacrossofOryzasativa(KMR3)×Oryzarufipogon(WR120)weregrownunderwellwateredconditions.Leafgasexchangemeasurementsandleafchlorophyllestimateswereconductedatthefloweringstage.Theresultsrevealedsignificantvariationsinnetphotosyntheticrate(Pn),transpirationrate(E),transpirationefficiency(Pn/E)andcarboxylationefficiency(Pn/Ci).PnshowedsignificantpositivecorrelationwithE,stomatalconductance(gs),Pn/Ciandtotalcanopydrymatter.SpecificleafareaandleafthicknesswerenotsignificantlycorrelatedwithPn.Thirty-sevenoutof40ILsshowedhigherPnthanKMR3[11.28μmol/(m2·s)],and20ILsshowedhigherPnthanWR120[15.08μmol/(m2·s)].ThelineIL194showedthehighestPn[21.62μmol/(m2·s)]withincreasedtotalcanopydrymatterfollowedbylinesIL381,IL106,IL363-12,IL198,IL86-18andIL50,whichexhibitedPnabove18.0μmol/(m2·s).TheILswithenhancedPnareapotentialsourcefordevelopingricevarietiesandhybridswithhigherbiomassandyield.

  • 标签: PHOTOSYNTHESIS WILD species RICE YIELD biomass
  • 简介:TheinteractionbetweenricehostanditspathogenXanthomonasoryzaepv.oryzae(Xoo)atcellularlevelwasstudiedbyusingaresistantsomaclonalmutantHX-3anditssusceptabledonorMinghui63.AfterinoculationwithXoostrainZhe173(Chinesepathotype|\).theactivityofsuperoxidedismutase(SOD)andperoxidase(POD)inthecallusofMinghui63wasincreaseddramatically,andtheactiveoxygen(O2^-)wasproducedatahigherrate;Meanwhile,thecallusgrewslowlywiththereductionofproteincontent.ComparedtotheactivityofSODandPOD.theproductionrateofO2^-andthefreshweightinHX-3callusvariedlittleaftertheinoculation.ItcouldbeproposedthatthereweregreatdifferencesbetweentheresistanceofHX-3andMighui63atcellularlevel.TherewasnodifferencedetectedconcerningresistancetobacterialleafblightinHX-3betweentheplantandthecallus.

  • 标签: 水稻 白叶枯病 抗病性 体细胞突变种 HX-3品种 细胞水平
  • 简介:到精选精英germplasms,从装饰用的梨树米饭栽培变种Wuyujing3导出的112异种被评估。象圆锥花序那样的收益部件每平方米数,谷物数字每圆锥花序,和谷物,重量被测量。象白垩的谷物(PCG)的百分比那样的优秀特点,稻谷收益(BRY),milled米饭收益(MRY),milling(DM)的度,直链淀粉内容(交流),蛋白质内容(PC),和在特点之中的关系是inverstigated。结果证明谷物产量每在谷物收益变化为94.64%贡献的平方米与6.4t/hm2的一平均数和谷物的数字从2.15~12.49t/hm2。为优秀特点,所有米饭异种有短尺寸(谷物长度≤5.5 ;公里)并且大胆形状(到宽度比率=1.10-2.00的谷物长度)。大多数米饭异种(87.5%)低于20%有PCG价值。有的MRY重视甚于50%的所有异种,低于20%的交流值,和低于10%的PC值。白垩的谷物的百分比否定地显著地与MRY被相关并且断然与DM相关。BRY和MRY否定地显著地与DM被相关。PC显著地并且断然与MRY被相关并且否定地与DM相关,当交流没这些优秀特点地有重要关联时。有25米饭异种,完成了象白垩的谷物的低百分比那样的江苏标准装饰用的梨树米饭的主要要求,这被结束,低直链淀粉满意的、最佳的蛋白质内容,并且它能被用作精英germplasms。因此,识别的异种可以在米饭质量的改进导致重要进步。

  • 标签: 产量构成因素 武育粳3号 粳稻品种 理化品质 产量变异 低直链淀粉含量
  • 简介:为了推进,改进高地大米变化Huhan3和Huhan7,种子样品为约1和5d与二艘可重获的飞船被送到外层空间并且分别地为7和5代被宣传。Phenotypic分析表明词法特点和蛋白质和谷物的直链淀粉内容变化了。由联系基因的简单顺序重复(SSR)和插入删除(InDel)的genomic变化的描述标记显示变化模式是很复杂的。大多数变化在简单顺序重复碎片发生在碎片的3结束或5结束。反向的抄写聚合酶链反应(RT-PCR)试金证明在SSR的那些部分的变化影响了他们的基因表示,显示那基因联系了标记将有用孤立功能的基因。为繁殖的地调查也表明有高产量,高质量和干旱忍耐的更多的线能通过太空繁殖被选择。结果显示太空mutagenesis为米饭繁殖导致了分子的变化,以及生理、词法的变化。

  • 标签: 分子变异 航天诱变 旱稻 简单重复序列 突变基因 应用
  • 简介:一张加倍的haploid(DH)人口由120根线组成了,源于在一indica变化,TN1,和一装饰用的梨树变化之间的一十字,Chunjiang06,被用来识别由使用QTLNetwork软件在胚芽和3-leaf-seedling阶段控制米饭寒冷忍耐的QTL。在4以后的正常胚芽的百分比

  • 标签: 苗期耐冷性 水稻秧苗 鉴定方法 QTL 胚芽 软件
  • 简介:Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.

  • 标签: 水稻科学 农业
  • 简介:ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.

  • 标签: RICE OsPIN1a green FLUORESCENT protein TRANSIENT
  • 简介:Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...

  • 标签:
  • 简介:米饭镉(Cd)敏感变异的cadB-1用Agrobacteriumtumefaciens被获得调停的系统。在cadB-1和野类型(WT)的暴露以后米饭幼苗到为有增加外部Cd集中的cadB-1和WT的10d,在根积累到高水平的Cd,茎和叶子的Cd集中的一范围,并且在cadB-1的幼苗生长的抑制比在WT更严肃。氢过氧化物累积在cadB-1的叶子和根是更高的。减少的谷胱甘肽(GSH)的比率/oxidized谷胱甘肽(GSSG),ascorbate(ASC)/dehydroascorbate(DHA)和减少的菸碱腺嘌dinucleotide磷酸盐(NADPH)/oxidized菸碱腺嘌dinucleotide磷酸盐(NADP+)在高Cd层次下面在叶子和根两都比在WT在cadB-1是更低的。ascorbateperoxidase(APX)的活动,谷胱甘肽peroxidase(GR),dehydroascorbatereductase(DHAR)和monodehydroascorbatereductase(MDHAR)在Cd的高水平的处理下面在叶子和根两都比在WT在cadB-1是也更低的。我们的结果建议在Cd应力下面,ASC-GSH周期更严重比在WT在cadB-1被禁止,显示变异的cadB-1不太能清除反应的氧种类并且对Cd敏感。

  • 标签: 脱氢抗坏血酸还原酶 还原型谷胱甘肽 镉(CD) 水稻幼苗 突变体 还原型烟酰胺腺嘌呤二核苷酸
  • 简介:实验被进行用荧光灯的微分显示器(软式磁碟机)方法在干旱应激和正常条件下面在米饭叶子和根比较信使rna表达式差别。一积极碎片被H.A的联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因,它有96%身份。cDNA是1423bp,并且包含了与345氨基酸残余编码蛋白质的1048bp的一完全的开的读物框架。而且,基因表示水平在正常条件下面比那在干旱应激下面是更高的。在干旱反应下面的NADPH氧化还原酶基因的可能的角色也被讨论。

  • 标签: 水稻 干旱压力 还原型辅酶II类氧化还原酶基因 克隆化 基因表达
  • 简介:到在米饭germplasm91-1A2的米饭胆量小蚊的抵抗被识别并且遗传上分析了。米饭人口的F1s从作为一男父母与米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)和Duokang1(Gm6)交叉的91-1A2被导出。到米饭胆量小蚊的所有父母线和F1,BC1F1和F2人口的抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2的易受影响的抵抗植物的分离比率被X2测试与1:3和9:7规则给予,建议到中国米饭胆量小蚊遗传因子型IV的91-1A2的抵抗被是新抵抗基因的二主导的基因控制,对已知的米饭胆量小蚊抵抗基因非突变而产生之遗传因子。

  • 标签: 水稻种质 遗传分析 稻瘿蚊 抗性鉴定 显性基因控制 F2群体
  • 简介:~~

  • 标签: