学科分类
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34 个结果
  • 简介:bispyribac钠10%SC和butachlor效果土壤上50%SC移植秋收作物米饭物理化学性质和microflora二个季节(2010和2011)被调查。体积密度,水成立能力,潮湿内容,土壤pH,有机物内容,电传导性,以及全部氮,可得到磷和可得到钾内容上除草剂效果与microflora人口(全部细菌,放射菌类和真菌)起被分析土壤没有重要变化物理化学性质被观察。除草剂处理开始微生物引起计数导致减少。可观时间以内应用除草剂降级,microflora人口甚至除草剂申请以后60d超过了起始计数。

  • 标签: 土壤理化性质 微生物种群 苯甲酸钠 SC 移植 双草醚
  • 简介:有低glutelin内容瑞斯作为肾失败影响病人功能食物合适。米饭低glutelin内容基因Lgc1二高度类似的glutelin基因GluB4和GluB5之间3.5-kb删除,它在染色体2短手臂上定位。低glutelin内容米饭改进选择效率繁殖,指定为InDel-Lgc1-1和InDel-Lgc1-2二个分子标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二个标记联合使用能容易把Lgc1遗传型不同米饭变化区分开来。作为种简单、便宜技术,因此,分子标记能广泛地被用来Lgc1基因识别不同变化并且低glutelin内容米饭帮助标记选择适用。

  • 标签: 蛋白基因 水稻育种 分子标记 蛋白含量 ORYZA PCR检测
  • 简介:Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.

  • 标签: 水稻科学 农业
  • 简介:ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.

  • 标签: RICE OsPIN1a green FLUORESCENT protein TRANSIENT
  • 简介:Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...

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  • 简介:米饭镉(Cd)敏感变异cadB-1用Agrobacteriumtumefaciens被获得调停系统。cadB-1和野类型(WT)暴露以后米饭幼苗有增加外部Cd集中cadB-1和WT10d,根积累高水平Cd,茎和叶子Cd集中个范围,并且cadB-1幼苗生长抑制比WT更严肃。氢过氧化物累积在cadB-1叶子和根是更高。减少谷胱甘肽(GSH)比率/oxidized谷胱甘肽(GSSG),ascorbate(ASC)/dehydroascorbate(DHA)和减少菸碱腺嘌dinucleotide磷酸盐(NADPH)/oxidized菸碱腺嘌dinucleotide磷酸盐(NADP+)高Cd层次下面叶子和根两个都比WTcadB-1是更低。ascorbateperoxidase(APX)活动,谷胱甘肽peroxidase(GR),dehydroascorbatereductase(DHAR)和monodehydroascorbatereductase(MDHAR)Cd高水平处理下面叶子和根两个都比WTcadB-1是也更低。我们结果建议Cd应力下面,ASC-GSH周期更严重比WTcadB-1被禁止,显示变异cadB-1不太能清除反应氧种类并且对Cd敏感。

  • 标签: 脱氢抗坏血酸还原酶 还原型谷胱甘肽 镉(CD) 水稻幼苗 突变体 还原型烟酰胺腺嘌呤二核苷酸
  • 简介:个实验被进行用荧光灯微分显示器(软式磁碟机)方法干旱应激和正常条件下面米饭叶子和根比较信使rna表达式差别。积极碎片被H.A联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因,它有96%身份。cDNA是1423bp,并且包含了345氨基酸残余编码蛋白质1048bp个完全读物框架。而且,基因表示水平正常条件下面比那干旱应激下面是更高干旱反应下面的NADPH氧化还原酶基因可能角色也被讨论。

  • 标签: 水稻 干旱压力 还原型辅酶II类氧化还原酶基因 克隆化 基因表达
  • 简介:米饭germplasm91-1A2米饭胆量小蚊抵抗被识别并且遗传上分析。米饭人口F1s从作为个男父母米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)和Duokang1(Gm6)交叉91-1A2被导出。米饭胆量小蚊所有父母线和F1,BC1F1和F2人口抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。BC1F1和F2易受影响抵抗植物分离比率被X2测试1:3和9:7规则给予,建议中国米饭胆量小蚊遗传因子型IV91-1A2抵抗被是新抵抗基因二主导基因控制,对已知米饭胆量小蚊抵抗基因非突变而产生之遗传因子。

  • 标签: 水稻种质 遗传分析 稻瘿蚊 抗性鉴定 显性基因控制 F2群体
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  • 简介:我们识别了米饭花机关发展异种,称为同样壳和男性无菌1(ohms1),从indicarestorer线子孙,Zhonghui801560Co光线照耀对待。ohms1异种展出了壳并且像词根并且象palea样组织花药和耻辱之间变换,它导致显示出‘tridentatelemma’ohms1异种小穗状花小穗。ohms1异种是完全无菌却有的60%-70%肥沃花粉。印射基因分析和基因证明ohms1被单个后退基因控制,并且变异基因对标记KY2和KY29之间染色体3短手臂上42-kb间隔印射罚款。在这个区域四个读物框架顺序分析表明异种第五intron最后底带了单个核苷酸转变(GA),它多半被通信到ohms1phynotype,种MIKC类型疯盒子基因OsMADS1(LOC_Os03g11614)。进步定序酶消化和cDNA显示可变拼接在MADS领域或氨基酸框架为ohms1,而是没有结构变化第六exon删除负责移动出现。另外,即时荧光灯量PCR分析证明OsMADS1表示水平ohms1异种显著地减少了。发展相关基因也显著地改变了米饭flowering因素和花表示层次。这些结果证明OsMADS1可以米饭起个重要作用花机关开发,特别地颖开发和小花原基区别。

  • 标签: 不育水稻 雄性不育 剪接因子 开颖 MADS-BOX基因 花器官发育
  • 简介:Thepromoterregionofadroughtandabscisicacid(ABA)induciblegene,osr40c1,wasisolatedfromasalt-tolerantindicaricevarietyPokkali,whichis670bpupstreamoftheputativetranslationstartcodon.Insilicopromoteranalysisofresultedsequenceshowedthatatleast15typesofputativemotifsweredistributedwithinthesequence,includingtwotypesofcommonpromoterelements,TATAandCAATboxes.Additionally,severalputativecis-acingregulatoryelementswhichmaybeinvolvedinregulationofosr40c1expressionunderdifferentconditionswerefoundinthe5′-upstreamregionofosr40c1.TheseareABA-responsiveelement,light-responsiveelements(ATCT-motif,BoxI,G-box,GT1-motif,Gap-boxandSp1),myeloblastosisoncogeneresponseelement(CCAAT-box),auxinresponsiveelement(TGA-element),gibberellin-responsiveelement(GARE-motif)andfungal-elicitorresponsiveelements(BoxEandBox-W1).Aputativeregulatoryelement,requiredforendosperm-specificpatternofgeneexpressiondesignatedasSkn-1motif,wasalsodetectedinthePokkaliosr40c1promoterregion.Inconclusion,thebioinformaticanalysisofosr40c1promoterregionisolatedfromindicaricevarietyPokkaliledtotheidentificationofseveralimportantstress-responsivecisactingregulatoryelements,andtherefore,theisolatedpromotersequencecouldbeemployedinricegenetictransformationtomediateexpressionofabioticstressinducedgenes.

  • 标签: 启动子序列 计算机分析 籼稻品种 分离 顺式调控元件 CAAT盒