简介：目的：确定黄酮对人角膜内皮细胞（humancornealendothelialcells,HCEC）的影响及其对因氧化损伤所致细胞凋亡的抗氧化保护作用。方法：利用体外培养的HCEC,通过添加不同浓度的黄酮继续培养,或经不同浓度黄酮预处理后再添加过氧化氢（H2O2）继续培养,采用光镜形态观察、吖啶橙/溴化乙锭（AO/EB）双染色和DNA琼脂糖凝胶电泳等技术手段,研究黄酮对HCEC的影响及其抗氧化作用。结果：黄酮浓度高于85μmol/L时可诱导HCEC发生细胞凋亡,而低于70μmol/L时对HCEC没有影响;55和70μmol/L黄酮预处理对600μmol/LH2O2所引起的HCEC细胞凋亡具有显著的抑制作用（60%→22%,8%;P〈0.01）,而85和100μmol/L黄酮预处理对H2O2所引起的HCEC细胞凋亡没有显著的抑制作用。结论：55～70μmol/L黄酮对HCEC具有显著的抗氧化保护作用,而浓度高于85μmol/L的黄酮反而能诱发HCEC发生细胞凋亡。

简介：·AIM:Toexploretheeffectofimmunizationwithcopolymer-1(COP-1)andretinalstemcells(RSCs)transplantationoninterferon-gamma(IFN-γ)levelsinaratexperimentalglaucomamodel.·METHODS:Anexperimentalglaucomawasinducedbyargonlaserphotocoagulationoftheepiscleralveinsandlimbalplexusintherighteyeofrats.Immediatelyfollowingglaucomainduction,ratswereimmunizedwithCOP-1.RSCswereculturedandtransplantedintravitreallyintotheeyesofglaucomamodelanimals1weekpost-lasertreatment.Sixexperimentalgroupswereused:COP-1/RSC,PBS/RSC,COP-1/PBS,PBS/PBS,glaucomamodelgroup,andanormalcontrolgroup.TheconcentrationofIFN-γinaqueoushumor(AH)andserumwasmeasuredbyenzyme-linkedimmunosorbentassay(ELISA)ineachofthesixgroups.Retinalganglioncell(RGC)survivalwasassessedbyquantifyingapoptosisusingHoechststaining.·RESULTS:ConcentrationsofIFN-γinAHandserumofratsthathadundergoneglaucomainductionwerehigherthanthoseofnon-inducedcontrolrats.TheconcentrationsofIFN-γinAHandserumoftheCOP-1/RSCstreatedgroupweredeterminedtobe2371.9ng/Land710.9ng/L,respectively,whichweresignificantlylowerthanthoseintheothertreatedgroups(P<0.05).Infact,IFN-γlevelsinthedualtreatedgroupwerereducedtobackgroundlevels.TheCOP-1/RSCgrouphadlowernumberofapoptoticRGCsthantheotherthreeexperimentalgroups(P<0.05).·CONCLUSION:ThereducedlevelsofIFN-γinAHandserumoftheCOP-1/RSCgroupmayberelatedtosynergisticeffectsbetweenRSCstransplantationandCOP-1immunemodulation.ItislikelythatthelowerlevelsofIFN-γpreventedRGCsglaucomatousapoptosis.·

简介：目的设计整体运动知觉检测方法和研究正常人运动知觉特性.方法设计整体运动知觉测试软件,由微机控制,测定20例(40眼)正常人的运动知觉阈值.结果正常人运动知觉功能与性别及眼别无相关关系,而大于与小于50岁组的运动知觉分数值分别为4.47±0.40、4.85±0.16,两者具有显著性差异.结论建立了整体运动知觉检测方法,为临床应用打下了基础.

简介：后囊膜混浊是白内障囊外摘除术后最主要的并发症之一.后囊膜混浊的发生是多因素参与的结果,手术方式的改进、人工晶状体的改良、术后炎症反应的控制都可以减少后囊膜混浊的发生.本文就人工晶状体的设计对后囊膜混浊的影响作一综述.

简介：目的：评价超声乳化白内障吸除联合人工晶状体植入术后人工晶状体眼的波前像差，讨论不同设计的人工晶状体对术后人工晶状体眼波前像差的影响。方法：选择年龄相关性白内障患者62例（69眼），年龄41～84（平均63．4±4．0）岁。其中男24例（28眼），女38例（41眼），右眼38例，左眼31例。随机平均分为3组，使年龄性别相匹配。其中A组植入三片式人工晶状体（ACRYsof^@MA60BM），B组植入一片式人工晶状体（ACRYsof^@SA60AT），C组植入蓝光滤过型一片式人工晶状体（ACRYsof^@SN60AT）。同一术者，同一超声乳化仪（Alcon^@INFINITIVISIONSYSTEM）和手术显微镜（CarlZeissStativS88），术中均采用角膜曲率最高经线上宽3．2mm长1．75mm的角巩膜缘隧道切口。术后1mo使用客观型波前像差仪（NidekOPD-scanARK-10000）进行波前像差检测，得出总体高阶像差的均方根（RMSh）。结果：A组三片式（ACRYsof^@MA60BM）的RMSh平均达到0．702±0．090μm，B组一片式（ACRYsof^@SA60AT）的RMSh平均达到0．529±0．067μm，C组蓝光滤过型一片式（ACRYsof^@SN60AT）的RMSh平均达到0．566±0．066μm。三组比较采用单向方差分析，5．0mm瞳孔大小时A组的总体像差值最高（P〈0．01），其余两组没有显著性差异（P=0．126）。组间差异有统计学意义。结论：人工晶状体襻的设计对超声乳化白内障吸除联合人工晶状体植入术后人工晶状体眼的像差有明显影响，但光学区染色对单色像差的影响无统计学差异。这一结果对进一步完善白内障手术以及人工晶状体材料和设计的改善提供了有意义的信息。

简介：目的探讨一组特殊设计镜片对周边屈光度、周边清晰视力范围和主观感受的影响。方法3例（4眼）被试者，年龄22-31岁，屈光度-2．0D，按随机顺序分别在单眼配戴普通非球面镜片、成长乐镜片、视特保大、中、小光区镜片的情况下进行周边屈光度、周边清晰视力范围测量和主观评分，主观评分包括远距和近距戴镜舒适度、清晰度和接受度。结果配戴5种镜片时，被试眼相对周边屈光度均为远视状态，远视度数随周边角度增加而增加。配戴普通非球面镜片和视特保大光区镜片时周边远视度数最大，视特保中光区镜片其次，成长乐镜片和视特保小光区镜片最小；配戴普通非球面镜片和视特保大光区镜片时的周边清晰视力范围最大，其次是视特保中光区镜片，最小的是成长乐镜片和视特保小光区镜片；远距评分为普通非球面镜片最高，视特保大光区镜片其次，然后为视特保中光区镜片，成长乐镜片及视特保小光区镜片最差；近距评分视特保大光区镜片接近普通非球面镜片，视特保中光区镜片和成长乐镜片其次，视特保小光区镜片最差。结论不同设计的中周部加光镜片对改变周边屈光度的作用存在差异，其配戴后的顺应性与镜片改变周边屈光度的程度呈负相关。

简介：Thekeratoprosthesis(KPro;artificialcornea)isaspecialrefractivedevicetoreplacehumancorneabyusingheterogeneousformingmaterialsfortheimplantationintothedamagedeyesinordertoobtainacertainvision.Themainproblemsofartificialcorneaarethebiocompatibilityandstabilityofthetissueparticularlyinpenetratingkeratoplasty.Thecurrentstudiesoftissue-engineeredscaffoldmaterialsthroughcomprisingcompositesofnaturalandsyntheticbiopolymerstogetherhavedevelopedanewwaytoartificialcornea.Althoughawideagreementthatthelong-termstabilityofthesedeviceswouldbegreatlyimprovedbythepresenceofcorneacells,modificationofkeratoprosthesistosupportcorneacellsremainselusive.Mostofthestudiesoncornealsubstratematerialsandsurfacemodificationofcompositeshavetriedtoimprovethegrowthandbiocompatibilityofcorneacellswhichcannotonlyreducethestimulusofheterogeneousmaterials,butalsomoreimportantlycontinuousandstablecorneacellscanpreventthedestructionofcollagenase.Thenecrosisofstromaandspontaneousextrusionofthedevice,allowformaintenanceofaprecornealtearlayer,andplaytheroleofensuringagoodopticalsurfaceandresistingbacterialinfection.Asaresult,improvementincornealcellshasbeenthemainaimofseveralrecentinvestigations;someefforthasfocusedonbiomaterialforitswellbiologicalpropertiessuchaspromotingthegrowthofcorneacells.Thepurposeofthisreviewistosummarythegrowthstatusofthecornealcellsaftertheimplantationofseveralartificialcorneas.

简介：AIM:Todescribethedesignandpreliminaryresultsofthehospitalbasedepidemiologicalstudyfordiabeticretinopathy(HBESDR),anongoingepidemiologicalstudytoestimatetheprevalenceofdiabeticretinopathy(DR)andtoelucidatetheclinical,anthropometric,biochemicalandanyotherriskfactorsassociatedwithdiabeticretinopathy.METHODS:Totally2000diabeteswillberecruitedfromtheDiabeteseyeclinicintheFirstAffiliatedHospitalofChinaMedicalUniversity.AllsubjectsunderwentbloodsugarestimationandOralGlucoseToleranceTesttodiagnosediabetes.Alldiabeteswouldundergocompletequestionnaire,acomprehensiveeyeexamination.Bloodandurinewouldbecollectedforbiochemicalinvestigations.AllfundusphotographsforanyDRwillbegraded.Participantswhoneedtreatmentwillbesenttotheophthalmicclinicandfollow-upintervalprogramforallsubjectswillbesuggested.Acomputerizeddatabaseiscreatedfortherecords.RESULTS:Todate,1174diabeteshavebeenrecruited,therewere350(29.81%)DRinalldiabetes,mostofthemwerewithmildnon-proliferativediabeticretinopathy(NPDR)(139,39.71%);71(20.29%)moderateNPDR,66(18.86%)severeNPDR,74(21.14%)proliferativediabeticretinopathy(PDR).Females,longerdurationofdiabetes,familyhistoryofdiabetesandhypertensionhadastatisticallysignificantincreaseinriskofanyDR.CONCLUSION:ThestudyisexpectedtoprovideanestimateoftheoverallprevalenceofDRandtheprevalencewithdifferentdurationofdiabetesandalsoabetterunderstandingoftheriskfactorsassociatedwithDR.

简介：AIM:Todemonstratethemorphologyandstructureofinvitroreconstructedtissue-engineeredhumancornealepithelium(TE-HCEP)withseedercellsfromanuntransfectedHCEPcellline.·METHODS:TheTE-HCEPswerereconstructedinvitrowithseedercellsfromanuntransfectedHCEPcellline,andscaffoldcarriersofdenudedamnioticmembrane(dAM)inair-liquidinterfaceculturefor3,5,7and9days,respectively.Thespecimenswereexaminedwithhematoxylin-eosin(HE)stainingofparaffin-section,immunocytochemicalstaining,scanningandtransmissionelectronmicroscopy.·RESULTS:DuringinvitroreconstructionofTE-HCEP,HCEPcellsformeda3-4,6-7and8-10layersofanHCEP-likestructureondAMsinair-liquidinterfaceculturefor3,5and7days,respectively.Butthecellsdeceasedto5-6layersandthestructureofstraifiedepitheliumbecamelooseatday9.Andthecellsmaintainedpositiveexpressionofmarkerproteins(keratin3andkeratin12),cell-junctionproteins(zonulaoccludens-1,E-cadherin,connexin43andintegrinβ1)andmembranetransportproteinofNa+-K+ATPase.TheHCEPcellsinTE-HCEPwererichinmicrovillionapicalsurfaceandestablishednumerouscell-cellandcell-dAMjunctionsatday5.·CONCLUSION:ThemorphologyandstructureofthereconstructedTE-HCEPweresimilartothoseofHCEPinvivo.TheHCEPcellsinthereconstructedTE-HCEPmaintainedthepropertiesofHCEPcells,includingabilitiesofformingintercellularandcell-extracellularmatrixjunctionsandabilitiesofperformingmembranetransportation.TheuntransfectedHCEPcellsanddAMscouldpromisinglybeusedinreconstructionHCEPequivalentforclinicalcornealepitheliumtransplantation.