简介:Thegrowth,biochemicalcontentandbioaccumulationquantityof2,2’,4,4’-tetrabromodiphenylether(BDE-47)inSkeletonemacostatumwerestudiedunderdifferentN:Pratios(1,4,16,64and128).AllcellularbiochemicalcontentsofS.costatumpresenteddecreasingtrendovercultivationtime.Atearlystageofcultivation,thecellularprotein,carbohydrateandlipidinS.costatumpresentedhighervaluesintreatmentsofN:P=4and16.However,theywerelowerinthesetreatmentsatthelatestage,buthigherintreatmentsN:P=1and128.Similarly,BDE-47levelspercellofS.costatumwerehigherintreatmentsofN:P=4and16atearlystageofcultivation,whichwere3.8and3.7ng(106cells)-1,respectively.Atthemiddlestageofcultivation,theBDE-47levelperS.costatumcelllowered;anditfurtherreducedinthetreatmentsofN:P=4and16atthelatestagewiththevalues0.6and0.5ng(106cells)-1,respectively.However,itroseinN:P=128,reachingupto2.3ng(106cells)-1.ComparedwithBDE-47percell,BDE-47peralgalvolumeunderdifferentN:Pratiosdidnotpresentobviousdifference.ThequantityBDE-47accumulatedpercellofS.costatumwaspositivelycorrelatedwithprotein,carbohydrateandlipidpercell;meanwhile,theBDE-47pervolumehadapositivecorrelationwithbiochemicalcontentpervolume.ThevariationofbioaccumulationabilityofBDE-47inS.costatumcanbeexplainedbybiochemicalchangesduetoN:Pratios.
简介:Thetyphoon,asamaturetropicalcyclonethatdevelopsinthewesternpartoftheNorthPacificOceanwithhighwindspeedandheavyrainfall,isoneofthemostlethalandcostlyofnaturaldisastersforthedenselypopulatedcountriesofEastAsia.Itcanbeeasilydetectedbyspace-bornesensorsoperatedatmicrowave,visibleorinfraredbands(Liuetal.,2014).SyntheticApertureRadar(SAR)is
简介:腐蚀抵抗无电在钠氯化物答案从12%~14%与磷的内容捏存款被学习。存款沉浸于3.5%NaCl答案让29d获得电气化学的参数;分别地为15d在标准盐水花测试被检验。存款的腐蚀抵抗被potentio动态的扫描学习,电气化学的阻抗光谱学(EIS),X光检查衍射(XRD);扫描电子显微镜学(FE-SEM)的冷地的排放与一个精力装备了散X光检查察觉者(EDX)。XRD的模式;FE-SEM的结果证明准备存款是非结晶的。但是在15d标准盐水花测试以后,一些针孔出现在存款的表面上;存款的表面上的磷的重量内容更高(它对钝化电影的形成有益)当因为镍的溶解重量比磷的大,镍内容更低时,比那,在标准盐前,水花测试。从potentio动态的扫描的结果;EIS证明钝化电影在NaCl答案在沉浸以后在Ni-P存款上形成了,它减少了腐蚀率捏样品。这个工作的结果在海洋的腐蚀显示出他们的潜在的应用。
简介:CytochromeP450(CYP)superfamilyisoneofthemembershiplargestandfunctionmostdiverseproteinsuperfamilyrecogniozedamonglivingbeings.Membersofthissuperfamilywerefurtherassignedtodifferentfamiliesandsubfamiliesbasedontheiraminoacidsimilarities.Accordingtotheirphylogeneticrelationships,theCYPgeneswhichlikelydivergedfromcommonancestorgeneandmaysharecommonfunctionsweregroupedintooneclan.Widelydistributingscallopsareagroupofthemostconspicuousbivalve;howeverthestudiesontheirCYPisacarce.Inthisstudy,wesearchedthegenomeandexpressedsequencetagsofZhikongscallop(Chlamysfarreri)forCYPgenes.Intotal,88non-redundantCYPwereidentified,whichwerehomedin13CYPsgenefamilies.Phylogeneticanalysisdividedthesegenesinto4CYPclans.Asindeuterostomes,Clan2wasthelargest,whichcontained33genesbelongingtoCYP1,CYP2,CYP17andCYP356families.Clan3contgained19genesbelongingtoCYP3,CYP5andCYP30families.Clan4contained23genes,allbelongingtoCYP4family.ThemitochondrialCYPclancontained9genesbelongingtoCYP10andCYP24families.Incomparison,protostomes(C.farreri,D.pluex,D.melanogaster)containedmoreCYPgenesthandeuterostomes(S.purpuratusandvertebrates)inClan2butlessgenesinClan3andClan4.OurfindingswillaidtodecipheringCYPfunctionandevolutioninscallopsandbivalves.
简介:Comparedtotowedstreamers,ocean-bottomseismometers(OBS)obtainbothS-wavedataandricherwavefieldinformation.Inthispaper,theinducedpolarizationmethodisusedtoconductwavefieldseparationonOBSdataobtainedfromtheShenhuareaintheSouthChinaSea.AcomparisonofthechangesinP-andS-waves,andacomprehensiveanalysisofgeologicalfactorswithinthearea,enableanalysisanddescriptionoftheoccurrenceofnaturalgashydrateinthestudyarea.ResultsshowanincreaseinP-wavevelocitywhennaturalgashydrateexistsintheformation,whereastheS-wavevelocityremainsalmostconstant,asS-wavescanonlypropagatethroughtherockskeleton.Therefore,thebottom-simulatingreflection(BSR)responseoftheP-waveisbetterthanthatoftheS-waveinthefrequencyanalysisprofile.Inawide-anglesection,therefractivewaveofthehydratelayerisevidentwhenusingP-wavecomponentsbutidentificationisdifficultwithS-wavecomponents.ThisvelocitymodelillustratesthesensitivityofPandS-wavecomponentstogashydrate.TheuseofthispolarizationmethodandresultsofanalysisprovidetechnicalandtheoreticalsupportforresearchonhydratedepositsandothergeologicalfeaturesintheShenhuarea.
简介:Astheexplorationandexploitationofoilandgasproliferatethroughoutdeepwaterarea,therequirementsonthereliabilityofdynamicpositioningsystembecomeincreasinglystringent.Thecontrolobjectiveensuringsafetyoperationatdeepwaterwillnotbemetbyasinglecontrollerfordynamicpositioning.Inordertoincreasetheavailabilityandreliabilityofdynamicpositioningcontrolsystem,thetripleredundancyhardwareandsoftwarecontrolarchitecturesweredesignedanddevelopedaccordingtothesafespecificationsofDP-3classificationnotationfordynamicallypositionedshipsandrigs.Thehardwareredundantconfigurationtakestheformoftriple-redundanthotstandbyconfigurationincludingthreeidenticaloperatorstationsandthreereal-timecontrolcomputerswhichconnecteachotherthroughdualnetworks.Thefunctionofmotioncontrolandredundancymanagementofcontrolcomputerswereimplementedbysoftwareonthereal-timeoperatingsystemVxWorks.Thesoftwarerealizationoftaskloosesynchronization,majorityvotingandfaultdetectionwerepresentedindetails.Ahierarchicalsoftwarearchitecturewasplanedduringthedevelopmentofsoftware,consistingofapplicationlayer,real-timelayerandphysicallayer.ThebehavioroftheDP-3dynamicpositioningcontrolsystemwasmodeledbyaMarkovmodeltoanalyzeitsreliability.Theeffectsofvariationinparametersonthereliabilitymeasureswereinvestigated.Thetimedomaindynamicsimulationwascarriedoutonadeepwaterdrillingrigtoprovethefeasibilityoftheproposedcontrolarchitecture.
简介:源于大气的碳的升起的层次,海洋使发酸成为了一个全球问题。它在海洋的有机体的发展,幸存,生长和生理学有重要影响。因此,一个高精确的传感器着急地被需要测量海水的pH。有0.25公里的一条直径的铱电线被用作底层,和红外/红外(哦)xpH电极被一出一步舞准备在在房间温度的一个LiOH答案的电气化学的方法。扫描电子显微镜(SEM)观察表明它与nanoscale粒子是涂的。在实验室测试,电极展出很有希望的pH回答,与理想的Nernst在测试pH的斜坡(56.14-59.52),快反应,好稳定性和长寿命缓冲答案。为海试用,四个pH电极和电极Ag/AgCl参考电极与一个白手起家的化学药品传感器,和将近70m的侧面察觉是综合的在Newport附近被实现港口,加利福尼亚在2015年8月3日。结果思考pH价值由传感器测量了,这离加CTD的海鸟911给的数据很靠近,与从的差别价值0.000075~0.064719。并且传感器显示出在0-40m水深度匹配度的一个更好的数据。另外,传感器的高精确和精确性使在海洋观察地里使用可能。
简介:Huanghe河三角洲是世界的大河之一,自从最后,Huanghe河里的Huanghe河三角洲paleoenvironmental进化是一个热问题冰川。基于联合AMS14核心DYZK1沉积标明日期在的C在亚底部侧面上Huanghe河三角洲和声学的顺序沉没了,phytolith分析在96件沉积样品上被执行。谷物尺寸参数,磁性的危险性与biostratum的垂直变化被相结合重建在Huanghe河三角洲的paleo沉积、气候的条件。在索引参数有一条重要垂直变化法律的学习结果表演,和Huanghe河三角洲的那沉积环境经历了河的facies-sealand转变的进化进程外形潮汐的完全外形浅海的faciesdelta外形自从26.0kaB。P...phytolith分析结果如下。第一,phytoliths被划分成矛尖状,光滑伸长,多刺伸长,一种特殊格律,长矩形,bulliform和其它类型。在不同sedimental环境,phytolith内容定期变化,显示phytolith在沉积环境有的一样装配稳定性的某个度。第二,矛尖状,phytoliths的光滑伸长和多刺伸长范畴在潮汐的扁平的外形和三角洲免职有更大的内容,当时一在浅海的外形的更小的内容和河的外形环境。通过变化系数的比较分析,第三,在沉积的主要phytolith范畴的内容在sealand转变外形和河的外形免职有一个更大的变化振幅,当在tidalflat外形和三角洲外形免职是更稳定的时。
简介:Outerdensefibersarespecificsubcellularcomponentsofmammalspermflagella,apartfromtheaxonemeanditsassociatedproteins.Asoneoftheouterdensefiberscomponents,ODF3isessentialfortheformationofmammalspermflagella.Inthepresentstudy,weisolatedtheOdf3gene,1033bpinlength,fromZhikongscallopChlamysfarreri,whichwasnamedasCf-Odf3.ItwaspredictedthatCf-Odf3encodesaproteinof240aminoacidswhichcontains5discontinuousPro-Gly-Prorepeats.TheCf-Odf3transcriptsweredetectedinbothtestisandovaryofC.farreriatvariousstagesthroughqRT-PCR.TheCf-ODF3proteinsynthesizedbyprokaryoteswaspurifiedusingNi2+-NTAaffinitychromatographyandusedtoproduceitspolyclonalantibody.TheantibodyproductwaseffectiveandspecificaswasexaminedbyWesternblotting.TheCf-Odf3transcriptandCf-ODF3proteinwerelocatedinintragonadalsomaticcells(ISCs),spermatogoniaandspermatocytesofC.farreritestes,whilenotranscriptionofCf-Odf3andsynthesisofCf-ODF3weredetectedinspermatidsandspermatozoa.ThelocationofOdf3expressionisdifferentfromthatofvertebrateswhereODF3issynthesizedspecificallyinspermatidsandspermflagella.Moreover,theCf-Odf3expressionwasalsodetectedinISCs,oogoniaandoocytesofthescallopovaries.OurfindingrevealedadifferentcharacteristicofOdf3expressionbetweenscallopandvertebrates,whichimpliedthatCf-Odf3playedaroleinthegametogenesisofC.farreri.
简介:EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.
简介:Combinedmulti-bodydynamicswithstructuraldynamics,anewdiscreteelementwithflexibleconnector,whichisapplicablefor3-Dbeamstructures,isdevelopedinthispaper.Boththegeneralizedelasticcoefficientmatrixoftheflexibleconnectorandthemassmatrixofdiscreteelementmaybeoff-diag-onalinageneralcase.Thezero-lengthrigidelementisintroducedtosimulatethenodeatwhichmultipleel-ementsarejointedtogether.Itmayalsobeeffectivewhentheaxesofadjacentelementsarenotinthesameline.Theexamplesforeigenvaluecalculationshowthatthemodelissuccessful.Itcanbeextendedtothegeometricnonlinearresponseanalysis.
简介:TiO2filmswereformedonmetallictitaniumsubstratesbytheanodicoxidationmethodinH2SO4solutionunderthe80VD.C..PhasecomponentandmicrostructurewerecharacterizedbyX-raydiffraction(XRD)andscanningelectronmicroscopy(SEM).Watercontactanglesontitaniumoxidefilmsurfaceweremeasuredunderbothdarkandsunlightilluminationconditions.Corrosiontestswerecarriedoutinseawaterunderdifferentilluminationconditionsbyelectrochemistryimpedancespectrum(EIS)andpolarizationcurves.TheresultshowedthattheTiO2filmpreparedbytheanodicoxidationmethodwasanatasewithauniformstructureandwithoutobviousporesorcracksonitssurface.Theaveragewatercontactangleofthefilmwas116.4?indark,incontrasttoanangleof42.7?undertheUVilluminationfor2hours,whichdemonstratesgoodhydrophobicproperty.Theanti-corrosionbehavioroftheTiO2filmwasdecliningwiththeextendedimmersiontime.Underdarkconditions,however,thehydrophobicTiO2filmretardedthewaterinfiltratingintothesubstrate.Theimpedancechangedslowlyandthecorrosioncurrentdensitywas2ordersofmagnitudelowerthanthatwiththefilmilluminatedbysunlight.AllofthosementionedaboveindicatethattheTiO2filmpossessesmuchbetterperformanceunderdarkcondition,anditcanbeappliedasanengineeringmaterialunderdarkseawaterenvironment.
简介:AbundantevidencesofhighersealevelsfromJiangsuandFujiancoastshaveprovedamarinetransgressioneventduring30–40kaBP,suggestingthattherewasastagewithhighsealevelandawarmclimatewhenicesheetsshrankintheNorthernHemisphere.Thedurationof30–40kaBPspannedaperiodinthelateMarineIsotopeStage3(MIS3)andwasinnatureaninterstadialepochduringtheLastGlacialperiodoftheQuaternary.Differentfromtheglacialperiodwithacoldclimate,thismarinetransgressionconsideredasapenultimatehighersealevelduringtheQuaternaryremainsapuzzlethatwhytheevidenceiscontrarytotheQuaternaryglacialtheory.Itisimportanttounderstandsealevelrisefortheseareassensitivelyrespondingtotheglobalchangesinthefuture.Torecognizethekeyissuesonsealevelchanges,theeustaticsealevel(H_S)wasdefinedastheglaciation-climateforcedsealevels,andtherelativesealevelchange(H_R)wasdefinedasthatasealevelrecordwaspreservedinsedimentthatexperiencedmultiplesecondaryactionsoflandandseaeffects.Onthebasisasdefinedabove,weconstructedmulti-levelmodelsofclimate-drivenglacio-eustaticchangesandland-seasystems.Byintegratingdatasetsfromeightboreholecoresandprescribingtheboundaryconditions,wesimulatedthechangesofHSandHRintheEastChinaSeaandsouthernYellowSeaareasinthelateMIS3.Themarinetransgressionstratafromtheboreholecoredatawasidentifiedatca.30mbelowpresentsealevelasaresultofthecollectiveinfluenceoficemeltingwater,neotectonicsubsidence,sedimentcompactionandterrestrialsedimentfillingsinceca.35kaago,whereasthesimulatedrelativesea-levelsturnedouttobe–26.3––29.9ma.s.l.Thesmallerrorinvolvedinthesimulationresultsof±(2.5–4.5)mdemonstratedthecredibilityoftheresults.OurresultsindicatedthatsealevelchangeinthelateMIS3wasdominatedbyglacialeffects,inwhichtheeustaticsea-levelwasbetween–19.2�
简介:FattyacidcompositionsoftwostrainsofmarinediatomsNitzschiaclosterium(MACCB222)andChaetocerosgracilis(MACCB13)havebeenexamined.ThemicroalgaehavebeengrownatdifferentinitialNaNO3concentrations(75,375,750,1125,1500,1875mg/L)andharvestedatthelateexponentialphase.TheresultsbyonefactoranalysisofvarianceshowthattheNaNO3concentrationshavenosignificantinfluenceontherelativegrowthrateoftwostrainsofmarinediatoms;theinfluenceofNaNO3concentrationsonthefattyacidsdiffersfromspeciestospecies.Themajorfattyacidsoftwodiatomstrainsare14:0,16:0,16:ln-7and20:5n-3(EPA),B13alsohashighpercentageof20:4n-6(6.0~9.1%).
简介:CarboncycleisconnectedwiththemostimportantenvironmentalissueofGlobalChange.Asoneofthemajorcarbonreservoirs,oceansplayanimportantpartinthecarboncycle.Inrecentyears,ironseemstogiveusagoodnewsthatoceanicironfertilizationcouldstimulatebiologicalproduc-tivityasCO2sinkofhuman-producedCO2.Oceanicironfertilizationexperimentshaveverifiedthataddingironintohighnutrientlowchlorophyll(HNLC)seawaterscanincreasephytoplanktonproductionandexportorganiccarbon,andhenceincreasecarbonsinkofanthropogenicCO2,toreduceglobalwarming.Insixtydays,theexportorganiccarboncouldreach10000timesforaddingironbymodelpredictionandinsituexperiment,I.e.TheatmosphericCO2uptakeandinorganiccarbondrawdowninupperseawatersalsohavethesamemagnitude.Therefore,oceanicironfertilizationisoneofthestrate-giesforincreasingcarbonsinkofanthropogenicCO2.Thepaperisfocusedontheironfertilization,es-peciallyinsituoceanironexperimentsinorderthatthefutureresearchismoreefficient.
简介:Astochastictwo-dimensionalFornasini-Marchesini'sModelⅡ(2-DFMMⅡ)withmultiplicativenoiseisgiven,andafilteringalgorithmforthismodel,whichisoptimalinthesenseoflinearminimum-variance,isdeveloped.Thestochastic2-DFMMⅡwithmultiplicativenoisecanbereducedtoa1-Dmodel,andtheproposedoptimalfilteringalgorithmforthestochastic2-DFMMⅡwithmultiplicativenoiseisobtainedbyusingthestateestimationtheoryof1-Dsystems.Anexampleisgiventoillustratethevalidityofthisalgorithm.
简介:Thispaperpresentsthedetaildesignconditions,designcriteriaandmethodsaswellasdesignconclusionsoftheprocess,structureandcorrosionoftheproductionflowlinesandhighpressurewaterinjectionlinesinBZ34-2/4oilfieldinBohaiBay.AtpresentthisdesignrepresentsthedesigncapabilityandlevelinthefieldofthesubmarinepipelineengineeringofChina.