Objective:TumorassociatedantigenencodinggeneHCA520(AF146019)wasidentifiedbyscreeningahumanhepatocellularcarcinomaexpressingcDNAlibraryusingSEREXtechnique.InthisexperimentwestudiedtheeffectofHCA520oncellproliferationandapoptosis.Methods:GeneHCA520wasgainedbyPCRandtransfectedinto293cells.ThestableexpressioncellswereobtainedbyG418selection.Thecellproliferationwasmeasuredby[3H]-TdRuptakeandapoptosisassaywasmeasuredbyFACS.Results:EukaryoticexpressionplasmidpcDNA3-HCA520wasconstructedanditsstabletransfectantswereobtained.OverexpressionofHCA520inhibitedthecellproliferationandenhancedcellapoptosisafterserumdeprivation.Conclusion:HCA520isanoveltumorassociatedantigenthatcanaffectcellproliferationandapoptosis.
