STUDY ON IMMOBILIZED PORCINE PANCREATIC LIPASE CATALYZING TRANSESTERIFICATION BETWEEN METHYL—BUTYRATE AND 1—BUTANOL IN NONAQUEOUS SYSTEMS

(整期优先)网络出版时间:1996-01-11
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Transesterificationbetweenmethyl-butyrateand1-butanolinnonaqueoussystemswascatalyzedbyporcinepancreaticlipasewhichwasimmobilizedoncross-linkedpolystyrene.Organicsolvents,substrateconcentration,contentsofwaterandotherparameterswhichaffecttheimmobilizedenzymeactivitywerestudied.Lipaseimmobilizedonhydrophobiccrosslinkedpolystyrenecanreduceitsdiffusionlimitinthereaction.Itwasfoundthattheactivityofimmobilizedlipaseinorganicsystemswastwotimesashighasthatoffreelipase.