摘要
Awoofer–tweeteradaptiveopticalstructuredilluminationmicroscope(AOSIM)ispresented.Bycombiningalow-spatial-frequencylarge-strokedeformablemirror(woofer)withahigh-spatial-frequencylow-strokedeformablemirror(tweeter),weareabletoremovebothlarge-amplitudeandhigh-orderaberrations.Inaddition,usingthestructuredilluminationmethod,ascomparedtowidefieldmicroscopy,theAOSIMcanaccomplishhighresolutionimagingandpossessesbettersectioningcapability.TheAOSIMwastestedbycorrectingalargeaberrationfromatriallensintheconjugateplaneofthemicroscopeobjectiveaperture.TheexperimentalresultsshowthattheAOSIMhasapointspreadfunctionwithanFWHMthatis140nmwide(usingawaterimmersionobjectivelenswithNA=1.1)aftercorrectingalargeaberration(5.9μmpeak-to-valleywavefronterrorwith2.05μmRMSaberration).Afterstructuredlightilluminationisapplied,theresultsshowthatweareabletoresolvetwobeadsthatareseparatedby145nm,1.62×belowthediffractionlimitof235nm.Furthermore,wedemonstratetheapplicationoftheAOSIMinthefieldofbioimaging.Thesampleunderinvestigationwasagreen-fluorescentprotein-labeledDrosophilaembryo.Theaberrationsfromtherefractiveindexmismatchbetweenthemicroscopeobjective,theimmersionfluid,thecoverslip,andthesampleitselfarewellcorrected.UsingAOSIMwewereabletoincreasetheSNRforourDrosophilaembryosampleby5×.
出版日期
2017年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
