In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction-中国期刊网

摘要 Micro-electrondiffraction(MicroED)isanemergingtechniquetousecryo-electronmicroscopetostudythecrystalstructuresofmacromoleculefromitsmicro-/nano-crystals,whicharenotsuitableforconventionalX-raycrystallography.However,thistechniquehasbeenpreventedforitswideapplicationbythelimitedavailabilityofproducinggoodmicro-/nano-crystalsandtheinappropriatetransferofcrystals.Here,wedevelopedacompleteworkflowtopreparesuitablecrystalsefficientlyforMicroEDexperiment.Thisworkflowincludesinsituon-gridcrystallization,single-sideblotting,cryo-focusionbeam(cryo-FIB)fabrication,andcryo-electrondiffractionofcrystalcryo-lamella.ThisworkflowenablesustoapplyMicroEDtostudymanysmallmacromolecularcrystalswiththesizeof2-10μm,whichistoolargeforMicroEDbutquitesmallforconventionalX-raycrystallography.Wehaveappliedthismethodtosolve2.5Acrystalstructureoflysozymefromitsmicro-crystalwithinthesizeof10×10×10μm^3.OurworkwillgreatlyexpandtheavailabilityspaceofcrystalssuitableforMicroEDandfillupthegapbetweenMicroEDandX-raycrystallography.

1Shengliu Wang;Shuoguo Li;Gang Ji;Xiaojun Huang;Fei Sun. Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ.生物物理学,2017-01.
2SUN Cheng Chen Haihua Zhang Jizhong Wei Hongmei Gu Zhongze. FABRICATION OF PHOTONIC CRYSTAL WITH SUPERLATTICES.高分子化学,2006-02.
3Jae Sung YOON;Tae-Jin JE;Doo-Sun CHOI;Sung Hwan CHANG;Kyung-Hyun WHANG. Fabrication of curved micro structures on photoresist layer.金属材料,2011-05.
4Li Zheng;Shi-Hong Zhang;Dirk Helm;Hong-Wu Song;Guang-Sheng Song;Neng-Yong Ye. Twinning and detwinning during compression–tension loading measured by quasi in situ electron backscatter diffraction tracing in Mg–3Al–Zn rolled sheet.金属材料,2015-10.
5ZHAOWen-yu ZHANGQing-Jie等. Determination of Space Group Pncm of Prehnite Using Electron Diffraction Method.材料科学与工程,2002-04.
6GaoshengYan;Jianguo Zhu;Yanlong Huang;Wenfen Hao;Yanjie Li. Fabrication of micro-scale gratings for moire method with a femtosecond laser.力学,2016-04.
7Zhi-xiang Cui;Hai-bin Zhao;Yi-yan Peng;Yottha Srithep;Lih-Sheng Turng;Chang-yu Shend. Fabrication of Highly Porous Interconnected Three-dimensional Scaffolds with Micro-channels.高分子化学,2014-07.
8杨尚磊;薛小怀;楼松年;芦凤桂. Investigation on gradient material fabrication with electron beam melting based on scanning track control.焊接,2007-03.
9Qiu-Gang Zong;Hui Zhang. In situ detection of the electron diffusion region of collisionless magnetic reconnection at the high-latitude magnetopause.空间物理学,2018-03.
10郭慧晶;张校亮;李晓春. Fabrication of ordered micro- and nano-scale patterns based on optical discs and nanoimprint.物理电子学,2016-04.

In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction

来源期刊

相关推荐

同分类资源更多

相关关键词

In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction

来源期刊

相关推荐

同分类资源 更多

相关关键词

同分类资源更多